Abstract:
:Macrophage precursor cells were cultured from the bone marrow of mice in a liquid culture system in the presence of conditioned medium. To separate their different maturation stages, they were passed through a discontinuous Ficoll density gradient, treated with iron plus magnet or passed through glass bead columns. The different maturation stages have been tested for their function in antibody-dependent cellular cytotoxicity (ADCC) against tumor target cells and in lymphokine-induced macrophage-mediated cytotoxicity. It is shown that the promonocyte, a nonadherent, nonphagocytic precursor cell, is a highly potent cytotoxic effector cell against antibody-coated tumor targets but is totally inactive as an effector cell in lymphokine-induced macrophage-mediated cytotoxicity. In contrast, in the lymphokine-induced macrophage-mediated cytotoxicity the cytotoxic effector cell is a mature macrophage. Thus, ADCC seems to be a function of the macrophage precursor promonocytes, whereas lymphokine-induced cytotoxicity is performed by mature macrophages. The relationship of promonocytes and killer (k) cells in ADCC against tumor targets is discussed.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Domzig W,Lohmann-Matthes MLdoi
10.1002/eji.1830090404subject
Has Abstractpub_date
1979-04-01 00:00:00pages
267-72issue
4eissn
0014-2980issn
1521-4141journal_volume
9pub_type
杂志文章abstract::Adenosine triphosphate has previously been shown to induce semi-mature human monocyte-derived dendritic cells (DC). These are characterized by the up-regulation of co-stimulatory molecules, the inhibition of IL-12 and the up-regulation of some genes involved in immune tolerance, such as thrombospondin-1 and indoleamin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737781
更新日期:2008-06-01 00:00:00
abstract::The inbred strain 13 guinea pig expresses at least three isotypes of immunoglobulin (Ig) lambda chain. The proportion of these isotypes in normal Ig can be estimated by isolating a constant (C) region cyanogen bromide peptide from lambda chains and quantitatively analyzing its component tryptic peptides. Anti-phenyltr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830081212
更新日期:1978-12-01 00:00:00
abstract::Plasmid cassette-transfer vectors pBHuC chi and pBHuC gamma l have been designed which enable the construction of recombinant baculoviruses directing the co-expression of complete immunoglobulin in insect cells. We describe the application of these vectors for the expression of a human/mouse chimeric monoclonal antibo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250731
更新日期:1995-07-01 00:00:00
abstract::Cells present in the polyoma virus-induced murine ascites tumor SEYF-a showed the capacity to fix soluble immune complexes of ovalbumin anti-ovalbumin. The fixation could be inhibited by preincubating the cells with antisera directed against H-2 antigens and with syngeneic anti-tumor antibodies. The latter did not rea...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060810
更新日期:1976-08-01 00:00:00
abstract::Co-infection of HCV with HIV has been associated with more rapid progression of HCV-related disease. HCV-specific T-cell immune responses, which are essential for disease control, are attenuated in co-infection with HIV. T-cell exhaustion has recently been implicated in the deficient control of chronic viral infection...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040340
更新日期:2010-09-01 00:00:00
abstract::A hallmark of parasite infection is the accumulation of innate immune cells, notably granulocytes and mast cells, at the site of infection. While this is typically viewed as a transient response, with the tissue returning to steady state once the infection is cleared, we found that mast cells accumulated in the large-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201646738
更新日期:2017-02-01 00:00:00
abstract::Injection of M-1 (Cmu), G-1 (Cgamma) heterozygous chickens on the day of hatch with anti-IgM-1 antiserum induced allotype suppression from which chickens recovered over a period of approximately 4 months. The suppression of the serum IgM-1 levels was matched by a decrease in the number of splenic and peripheral blood ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110407
更新日期:1981-04-01 00:00:00
abstract::Chlamydia pneumoniae stimulates potently maturation of and cytokine secretion by bone marrow-derived dendritic cells (BMDDC). BMDDC responses depend mainly on Toll-like receptor (TLR)2 and to a minor extent on TLR4. We demonstrate here using C. pneumoniae in an infectious model with the replication-permissive epitheli...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200209)32:9<2460::AID-IMMU2460>3
更新日期:2002-09-01 00:00:00
abstract::DT9301-0229737 the TcR are believed to provide the peptide fragments bound to major histocompatibility (MHC) molecules. TcR have an immunoglobulin (Ig)-like structure and, in an analogous manner to antigen recognition by Ig, the third complementarity determining regions (CDR3) of the TcR are believed to provide the pr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240519
更新日期:1994-05-01 00:00:00
abstract::We investigated the effect of recombinant murine interleukin 4 (IL 4) in the absence or presence of recombinant murine interferon-gamma (IFN-gamma) on adherent bone-marrow macrophages (M phi), peritoneal exudate and resident peritoneal M phi from susceptible BALB/c M phi, which were pulse-infected with Leishmania majo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210213
更新日期:1991-02-01 00:00:00
abstract::Natural antibodies (Ab) reacting with self antigens have been shown to be present in all individuals. These autoantibodies (auto-Ab) can be either pathogenic or non-pathogenic. Auto-Ab reacting with the alpha-subunit of the high-affinity receptor for IgE (FcepsilonRIalpha) have been implicated in the pathogenesis of a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199904)29:04<1149::AID-IMM
更新日期:1999-04-01 00:00:00
abstract::The antigen receptor of the T lymphocytes is one of the most complex eukaryotic membrane structures studied to date. The T cell receptor (TcR) consists of two disulfide-linked glycoprotein chains (alpha/beta or gamma/delta) and is noncovalently associated with a group of small and invariable CD 3 proteins. Four CD 3 c...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180508
更新日期:1988-05-01 00:00:00
abstract::Deficiencies in terminal complement components, including the component C7, are uncommon and associated with an increased risk of recurrent systemic neisserial infection. A total of 22 molecular defects have been reported in the C7 gene with both complete (C7Q0) and subtotal (C7SD) C7 deficiencies. In this study we re...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636812
更新日期:2007-05-01 00:00:00
abstract::Activation of invariant natural killer T (iNKT) cells by treatment with their α-galactosyl ceramide ligand provides therapeutic benefits in several immune inflammatory settings. Given the artificial nature of this stimulation, the natural regulatory functions of iNKT remain uncertain. Addressing this issue in a mouse ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040647
更新日期:2011-02-01 00:00:00
abstract::Chronic skin inflammation resulting from a defective epidermal barrier is a hallmark of atopic dermatitis (AD). We previously demonstrated that mice lacking FGF receptors 1 and 2 in keratinocytes (K5-R1/R2 mice) develop an AD-like chronic dermatitis as a result of an impaired epidermal barrier. Here, we show that γδ T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545675
更新日期:2015-09-01 00:00:00
abstract::Vitamin A and its metabolite retinoic acid influence various aspects of immunity. Although the capacity of vitamin A to condition intestinal CD103(+) DCs to imprint tissue-specific homing programs onto activated lymphocytes is well documented, it is unclear whether vitamin A also regulates DC populations in other tiss...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201343631
更新日期:2013-06-01 00:00:00
abstract::Riboflavin, also known as vitamin B2 , is converted by riboflavin kinase (RFK) into flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which are essential cofactors of dehydrogenases, reductases, and oxidases including the phagocytic NADPH oxidase 2 (Nox2). Riboflavin deficiency is common in young adul...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343940
更新日期:2014-03-01 00:00:00
abstract::The structure of immunological synapses formed between murine naive T cells and mature dendritic cells has been subjected to a quantitative analysis. Immunofluorescence images of synapses formed in the absence of antigen show a diffuse synaptic accumulation of CD3 and LFA-1. In electron microscopy, these antigen-free ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425857
更新日期:2005-06-01 00:00:00
abstract::Stem cell factor (SCF) is encoded at the Sl locus of the mouse and is the ligand for the c-kit receptor. Recombinant rat SCF164 (rrSCF164) induces proliferation and promotes maturation of mouse mast cells in vitro and in vivo and can also induce c-kit receptor-dependent mouse mast cell degranulation. We now report tha...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230415
更新日期:1993-04-01 00:00:00
abstract::An experimental model of adenosine deaminase deficiency was established on the human T cell line Jurkat by using 2'-deoxycoformycin, a strong specific inhibitor of the enzyme. When deoxyadenosine was added to the inhibited cells, the nucleotide profile was modified reproducing that found in lymphocytes from adenosine ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200322
更新日期:1990-03-01 00:00:00
abstract::Lymphocyte infiltration to pancreatic islets is associated to chemoattraction, as are other inflammatory autoimmune processes. We examined whether development of insulitis and diabetes depends on chemoattraction of lymphocytes via the CCR5 chemokine receptor. In non-obese diabetic (NOD) mice, a substantial fraction of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324285
更新日期:2004-02-01 00:00:00
abstract::Membrane-bound IgG was found only on old populations of platelets from normal individuals. This IgG could be dissociated from senescent cells by repeatedly heating the cells. Heat-eluted IgG (He-IgG) prepared from senescent red blood cells was capable of binding to either heat-treated old platelets or Vibrio cholerae ...
journal_title:European journal of immunology
pub_type: 杂志文章,收录出版
doi:10.1002/eji.1830131207
更新日期:1983-12-01 00:00:00
abstract::Using limiting dilution analysis, we investigated proliferative responses of UCHL1+ and UCHL1- T cell populations to compare the precursor cell frequencies following recall and alloantigen stimulation, and the complexity of cellular interactions within UCHL1+ and UCHL1- populations. We find high frequencies of recall ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181102
更新日期:1988-11-01 00:00:00
abstract::Twelve continuous rat tissue cultured mast cell (MC) lines were established by prolonged culture of rat peritoneal MC in the absence of added factors or feeder layers. Two of these lines, RCMC1 and RCMC2, have been briefly described previously, seven others are now also described. Both RCMC1 and RCMC2 lack a marker ch...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200127
更新日期:1990-01-01 00:00:00
abstract::Collagen type II-induced arthritis (CIA) is an experimental model of arthritis that has been successfully used to dissect the pathogenesis of human rheumatoid arthritis and to identify potential therapeutic targets. We have used this model to evaluate the role of T cell co-stimulation in both disease development and p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261008
更新日期:1996-10-01 00:00:00
abstract::T lymphocytes can be activated and induced to proliferate through stimulation of the CD2 glycoprotein with functional combinations of CD2 antibodies. However, this mechanism of signal transduction via CD2 is still not fully understood. We have investigated which molecules on the T cell surface preferentially associate...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200205)32:5<1509::AID-IMMU1509>3
更新日期:2002-05-01 00:00:00
abstract::Activated human monocytes are a source of numerous beta-chemokines. The present study was conducted to determine whether these cells produce the human beta-chemokine I-309 and to compare the induction requirements of I-309 to those of other beta-chemokines. We demonstrate that appropriately stimulated adherence-purifi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270317
更新日期:1997-03-01 00:00:00
abstract::Cross-linking of surface immunoglobulin (sIg) by antibodies against IgM, IgG and IgD activates B cells and in some circumstances can induce cell proliferation. We studied the potential link between anti-Ig-induced changes in the cytosolic free Ca2+ concentration ([Ca2+]i), inositol phosphate production and the ability...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171209
更新日期:1987-12-01 00:00:00
abstract::The purpose of this study was to obtain additional information on the mechanism by which interferon-gamma (IFN-gamma) is able to regulate gene expression in macrophages. The expression of the genes for class II histocompatibility I-A beta, tumor necrosis factor (TNF) and complement component C3 was assayed after treat...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190621
更新日期:1989-06-01 00:00:00
abstract::We have fractionated human tonsillar B cells on the basis of CD5 expression and determined the nucleotide sequences of immunoglobin light chain variable (V) regions encoded by the single member of the V chi 4 gene family in both CD5+ and CD5- populations. The majority of cDNA from both CD5+ and CD5- B cells population...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230636
更新日期:1993-06-01 00:00:00