Abstract:
:Using limiting dilution analysis, we investigated proliferative responses of UCHL1+ and UCHL1- T cell populations to compare the precursor cell frequencies following recall and alloantigen stimulation, and the complexity of cellular interactions within UCHL1+ and UCHL1- populations. We find high frequencies of recall antigen responses among UCHL1+, but not UCHL1- T cells. In contrast, both populations contain similar frequencies of alloantigen responsive cells. Our results are consistent with single-hit kinetics in recall as well as alloantigen responses and show no complex cellular interactions within the responding populations. In conclusion, the difference between the recall antigen response of UCHL1+ and UCHL1- cells observed in conventional proliferation assays is most probably due to a high frequency of recall antigen-responsive UCHL1+ cells and not to suppressive phenomena in the UCHL1- population. These data suggest that memory T cells are largely of the UCHL1+ phenotype. The relation of post-thymic T cell maturation and differential CD45 expression is briefly discussed.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Merkenschlager M,Terry L,Edwards R,Beverley PCdoi
10.1002/eji.1830181102subject
Has Abstractpub_date
1988-11-01 00:00:00pages
1653-61issue
11eissn
0014-2980issn
1521-4141journal_volume
18pub_type
杂志文章abstract::Mouse B cells cultured with either phorbol myristate acetate (PMA), or with Ca2+ ionophores enter a transitional activated state, between quiescence (G0) and G1, but do not synthesize DNA. It is shown here that the combination of PMA plus the ionophore ionomycin induces resting B cells to synthesize DNA, but not to se...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160118
更新日期:1986-01-01 00:00:00
abstract::A gene-encoding ligand for deletion of T cells bearing TcRV beta 6 and V beta 8.1 cosegregates a new mammary tumor provirus locus, Mtv-50 in NC mice. The sequence of the open reading frame (ORF) in the 3' long terminal repeat (LTR) of Mtv-50 was strikingly similar to those of Mtv-7, Mtv-43 and exogenous mouse mammary ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240235
更新日期:1994-02-01 00:00:00
abstract::The B cell line 721.174 has lost the ability to present intracellular antigens to major histocompatibility complex (MHC) class I-restricted cytotoxic T lymphocytes (CTL). This phenotype results from a homozygous deletion in the MHC that includes the peptide transporter genes TAP1 and TAP2, and the proteasome subunits ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250238
更新日期:1995-02-01 00:00:00
abstract::To investigate the role of interferon-gamma (IFN-gamma) in experimental autoimmune thyroidits (EAT), H-2k mice with a disrupted IFN-gamma receptor (IFN-gamma R) gene were immunized with porcine thyroglobulin (pTg). We observed that EAT occurred on day 19 and remitted on day 35 in IFN-gamma R-deficient (IFN-gamma R(0/0...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199801)28:01<201::AID-IMMU
更新日期:1998-01-01 00:00:00
abstract::Precursor CD4-CD8- (DN) thymocytes rearrange their TCR-beta genes, and only those which succeed in beta-selection subsequently expand and differentiate into immature CD4+CD8+ (DP) thymocytes. The cell subsets corresponding to the successive steps of this transition can be defined in terms of CD44 and CD25 expression. ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2001010)31:10<3038::aid-immu3038
更新日期:2001-10-01 00:00:00
abstract::The objective of the present investigation was to culture helper or suppressor/cytotoxic T cell subsets after extensive purification in interleukin 2-containing medium to determine whether phenotype changes for OKT4/91d6- or OKT8/Leu-2a-defined antigens do occur with prolonged culture. Repeated and sequential analysis...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140819
更新日期:1984-08-01 00:00:00
abstract::DNA vaccines induce CTL attack on target tumor epitopes, but tumor elimination in vivo also requires sufficient effector CTL to enter the site, guided by inflammatory chemokines. Many herpesviruses contain genes for chemokine and chemokine receptor-like proteins to protect infected cells from immune attack. To assess ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200212)32:12<3481::AID-IMMU3481>
更新日期:2002-12-01 00:00:00
abstract::Cellular metabolism is dynamically regulated in NK cells and strongly influences their responses. Metabolic dysfunction is linked to defective NK cell responses in diseases such as obesity and cancer. The transcription factors, sterol regulatory element binding protein (SREBP) and cMyc, are crucial for controlling NK ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048784
更新日期:2020-09-18 00:00:00
abstract::The capacity of human peripheral blood lymphocytes (PBL) to bind antigen-IgG antibody (EA(IgG)) and antigen-IgM antibody (EA(IgM)) complexes was investigated using a rosette technique with ox erythrocytes coated with rabbit IgG or IgM antibody. It was found that while EA(IgG)-rosette-forming cells (RFC) were detected ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050812
更新日期:1975-08-01 00:00:00
abstract::The migration of monocytes to sites of inflammation is largely determined by their response to chemokines. Although the chemokine specificities and expression patterns of chemokine receptors are well defined, it is still a matter of debate how cells integrate the messages provided by different chemokines that are conc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838906
更新日期:2009-04-01 00:00:00
abstract::The crucial role of interleukin (IL)-4 in the induction of murine IgG1 and IgE responses, which are coupled through the process of sequential isotype switching, has been well documented. Whereas IL-4 is obligatory for the induction of IgE responses, it enhances IgG1 responses. In this study, using neutralizing antibod...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240624
更新日期:1994-06-01 00:00:00
abstract::The monoclonal antibody W6/32.1 recognizes a public determinant on the HLA-A, B and C antigens of all tested human haplotypes. Though the antibody does not bind to normal mouse cells of any H-2 haplotype, it does show an unexpected specificity for the T cell leukemia line MBL-2 from a C57BL/6 mouse. It is shown that t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130213
更新日期:1983-02-01 00:00:00
abstract::Humanized BALB/c mice (termed trimera mice) conditioned by lethal total body irradiation and bone marrow transplantation from SCID mice have been described to support rapid engraftment of human peripheral blood mononuclear cells (PBMC) and the induction of strong B and T cell responses after immunization in vivo. More...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200107)31:7<2071::aid-immu2071>3
更新日期:2001-07-01 00:00:00
abstract::The cytokine interleukin 1 (IL 1) plays an important role in the induction of IL 2 secretion and high-affinity IL 2 receptor (IL 2R) expression by T cells. The events that follow binding of IL 1 to IL 1R, however, are still unknown. In this study we describe two variants of the murine thymoma EL4 (5D3 and D6/76) that ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190217
更新日期:1989-02-01 00:00:00
abstract::We previously showed that granulocyte-macrophage colony-stimulating factor (GM-CSF) breaks tolerance induction. The objective of this study was to determine whether GM-CSF breaks established inhalation tolerance. To induce tolerance, BALB/c mice were exposed to aerosolized ovalbumin (OVA) for 10 consecutive days. A co...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425012
更新日期:2004-09-01 00:00:00
abstract::In a search for proteins involved in signal transduction through the T cell receptor (TcR/CD3 complex), a recently developed highly efficient method for labeling of nucleotide binding proteins in permeabilized cells was applied. Here, we report that human CD3-gamma could be labeled by periodate-oxidized [alpha-32P] GT...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230224
更新日期:1993-02-01 00:00:00
abstract::B cells isolated from germinal centers (GC) of immune mice 2-5 days after antigen (Ag) challenge migrate in response to chemotactic signals, whereas GC B cells isolated at other times and resting B cells do not. Since B cells are in direct contact with follicular dendritic cells (FDC) in GC we reasoned that FDC might ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250437
更新日期:1995-04-01 00:00:00
abstract::T cell activation and cytokine secretion are important mediators of inflammation in allergic asthma. The costimulatory pathway CD28/CD80/CD86 has been shown to play an important role in T cell activation in allergic asthma, but less is known about the effect of other costimulatory molecules in allergy. The costimulato...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2002010)32:10<2874::AID-IMMU2874
更新日期:2002-10-01 00:00:00
abstract::A series of rat monoclonal antibodies (mAb) directed against the Thy-1 molecule have been evaluated for their ability to stimulate immature (CD4-8-) murine thymocytes. CD4-8- thymocytes could be induced to proliferate by several anti-Thy-1 mAb provided that exogenous interleukin 1 or interleukin 2 was provided. Appare...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171127
更新日期:1987-11-01 00:00:00
abstract::NLRP3 inflammasome is a protein complex crucial to caspase-1 activation and IL-1β and IL-18 maturation. This receptor participates in innate immune responses to different pathogens, including the bacteria of genus Brucella. Our group recently demonstrated that Brucella abortus-induced IL-1β secretion involves NLRP3 in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201848016
更新日期:2019-07-01 00:00:00
abstract::IL-10 is a potent anti-inflammatory molecule, which regulates TNF-alpha at multiple levels. We investigated whether IL-10 also modulated the activity of the TNF-alpha-converting enzyme (TACE). Using an ex vivo fluorogenic assay we observed that LPS rapidly induced TACE activity in monocytes coinciding with release of ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737821
更新日期:2008-04-01 00:00:00
abstract::Tumor necrosis factor (TNF) is a cytokine which, among other properties, is a principle mediator of inflammation and septic shock. It acts upon target cells by binding to specific cell surface receptors. A10G10 is a murine monoclonal antibody which recognizes human TNF and neutralizes its activity. A rabbit polyclonal...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221143
更新日期:1992-11-01 00:00:00
abstract::The antigen receptor of the T lymphocytes is one of the most complex eukaryotic membrane structures studied to date. The T cell receptor (TcR) consists of two disulfide-linked glycoprotein chains (alpha/beta or gamma/delta) and is noncovalently associated with a group of small and invariable CD 3 proteins. Four CD 3 c...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180508
更新日期:1988-05-01 00:00:00
abstract::Natural Killer (NK) cell-based immunotherapy is a promising approach to treat hepatocellular carcinoma (HCC). The mechanisms underlying the regulation of NK cell activity are not completely understood. In this research, we identified the expression of taste receptor type 1 member 1 (T1R1) and taste receptor type 1 mem...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201847688
更新日期:2018-12-01 00:00:00
abstract::Innate immunity, which is unable to discriminate self from allo-antigens, is thought to be important players in the induction of miscarriages. Here, we show that the administration of IL-12 to syngeneic-mated C57BL/6 mice on gestation day 7.5 (Gd 7.5), drives significant miscarriages in pregnant females. Furthermore, ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545923
更新日期:2016-08-01 00:00:00
abstract::Regulatory T (Treg) cells are a constitutively immunosuppressive subtype of T cells that contribute to the maintenance of immunological self-tolerance and immune homeostasis. However, the molecular mechanisms involved in the regulation of Treg cells remain unclear. In the present study, we identified ubiquitously expr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343394
更新日期:2014-02-01 00:00:00
abstract::Orally administrated iron is suspected to increase susceptibility to enteric infections among children in infection endemic regions. Here we investigated the effect of dietary iron on the pathology and local immune responses in intestinal infection models. Mice were held on iron-deficient, normal iron, or high iron di...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545642
更新日期:2015-09-01 00:00:00
abstract::Cytokines are the main agents known to regulate Th1 / Th2 commitment, where they may operate through paracrine activity within clusters of T cells gathered around dendritic cells (DC). An in vitro system is used here to test this possibility, using clusters around DC composed of naive TCR-transgenic ovalbumin peptide ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(200005)30:5<1255::AID-IMMU
更新日期:2000-05-01 00:00:00
abstract::The influence of Th2 cytokines in tuberculosis has been a matter of dispute. Here we report that IL-4 has a profound regulatory effect on the infection of BALB/c mice with Mycobacterium tuberculosis. Depletion of IL-4 with a neutralizing mAb caused only evanescent reduction of lung infection, but when combined with i....
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636764
更新日期:2007-03-01 00:00:00
abstract::Several drugs targeting members of the TNF superfamily or TNF receptor superfamily (TNFRSF) are widely used in medicine or are currently being tested in therapeutic trials. However, their mechanism of action remains poorly understood. Here, we explored the effects of TNFRSF co-stimulation on murine Foxp3+ regulatory T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201948393
更新日期:2020-07-01 00:00:00