Preservation of the helper and suppressor/cytotoxic T-lymphocyte phenotype in continuous culture.

Abstract:

:The objective of the present investigation was to culture helper or suppressor/cytotoxic T cell subsets after extensive purification in interleukin 2-containing medium to determine whether phenotype changes for OKT4/91d6- or OKT8/Leu-2a-defined antigens do occur with prolonged culture. Repeated and sequential analysis of such polyclonally activated cultured T cells documented stability of the OKT4/91d6+ and OKT8/Leu-2a+ phenotype. Both populations could be considerably expanded in culture. However, mixing experiments revealed a growth advantage of the OKT8/Leu-2a+ subset. A significant number of cells expressing both phenotypic markers was not detectable. While cultured T cells promoted on interleukin 2 were stable for expression of these functionally important differentiation markers, additional studies on activation antigens revealed high numbers of Tac+ cells at an early time of culture and decreasing numbers during further incubation. The expression of Tac antigen preceded the exponential expansion of the culture. In contrast, the number of cells bearing Ia determinants steadily increased with prolonged culture.

journal_name

Eur J Immunol

authors

Gramatzki M,Baum W,Burmester GR,Kalden JR

doi

10.1002/eji.1830140819

subject

Has Abstract

pub_date

1984-08-01 00:00:00

pages

762-5

issue

8

eissn

0014-2980

issn

1521-4141

journal_volume

14

pub_type

杂志文章
  • Human monocyte cytotoxin is not identical with lymphoblastoid lymphotoxin.

    abstract::Mononuclear phagocytes and lymphocytes can both synthesize cytotoxins which kill certain tumor cell lines and recent evidence suggests that these cytotoxins may be identical. In this study, human monocyte cytotoxin and lymphoblastoid lymphotoxin have been critically compared in terms of specificity, physicochemical ch...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830150321

    authors: Matthews N

    更新日期:1985-03-01 00:00:00

  • Spontaneous and ligand-induced endocytosis of CD23 (Fc epsilon receptor II) from the surface of B lymphocytes generates a 16-kDa intracellular fragment.

    abstract::It has been reported that the 45-kDa low-affinity Fc epsilon receptor (Fc epsilon RII) on B cells is cleaved spontaneously from the cell surface to release soluble fragments. This study demonstrates an additional fate of the Fc epsilon RII. 125I-labeled CD23+ B cells were cultured for 24 h at 37 degrees C. After lysis...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830220634

    authors: Grenier-Brossette N,Bourget I,Akoundi C,Bonnefoy JY,Cousin JL

    更新日期:1992-06-01 00:00:00

  • Functional maturation of recent thymic emigrants in the periphery: development of alloreactivity correlates with the cyclic expression of CD45RC isoforms.

    abstract::The transition from fully developed CD4+CD8- single-positive (SP) thymocytes into fully mature recirculating peripheral T cells is both poorly understood with regard to the expression of restricted isoforms (CD45R) of the leukocyte common antigen and in terms of T cell function. The present investigation monitored the...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830220913

    authors: Yang CP,Bell EB

    更新日期:1992-09-01 00:00:00

  • Human CD8(+) T cells expressing HLA-DR and CD28 show telomerase activity and are distinct from cytolytic effector T cells.

    abstract::Cycling lymphocytes may express the enzyme telomerase which is involved in maintenance of telomere length and cell proliferation potential. In CD8(+) T cells freshly isolated from peripheral blood, we found that in vivo cycling cells expressed HLA-DR. Furthermore, CD28-positive cells are known to have longer telomeres...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/1521-4141(200102)31:2<459::aid-immu459>3.0

    authors: Speiser DE,Migliaccio M,Pittet MJ,Valmori D,Liénard D,Lejeune F,Reichenbach P,Guillaume P,Lüscher I,Cerottini JC,Romero P

    更新日期:2001-02-01 00:00:00

  • Epratuzumab modulates B-cell signaling without affecting B-cell numbers or B-cell functions in a mouse model with humanized CD22.

    abstract::Treatment of systemic lupus erythematosus patients with epratuzumab (Emab), a humanized monoclonal antibody targeting CD22, leads to moderately reduced B-cell numbers but does not completely deplete B cells. Emab appears to induce immunomodulation of B cells, but the exact mode of action has not been defined. In the p...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201646383

    authors: Özgör L,Brandl C,Shock A,Nitschke L

    更新日期:2016-09-01 00:00:00

  • The specificity and efficiency of endogenous peptides in the induction of HLA class I alpha chain refolding.

    abstract::The specificity and efficiency of endogenous peptides in the HLA class I binding have been investigated by the use of a simple procedure that is based on the serological detection of the folding of HLA class I alpha chains that is induced by the binding to specific peptides in the presence of beta 2 microglobulin. HLA...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830220834

    authors: Tanigaki N

    更新日期:1992-08-01 00:00:00

  • Categorical selection of the antibody repertoire in splenic B cells.

    abstract::In the bone marrow, the passage of developing B cells through critical checkpoints of differentiation is associated with a reduction of specific categories of CDR3 of the Ig heavy chain (CDR-H3), particularly those with excessive hydrophobic or charged amino acids and those with a length of eight or fewer residues. To...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200636569

    authors: Schelonka RL,Tanner J,Zhuang Y,Gartland GL,Zemlin M,Schroeder HW Jr

    更新日期:2007-04-01 00:00:00

  • Selective enhancement of B cell antigen receptor-mediated antigen presentation by treatment with transforming growth factor-beta.

    abstract::TGF-beta1 was examined for the ability to regulate Ag-presentation by B cells, using A20-HL B lymphoma cells bearing TNP-specific IgM receptors. Treatment of A20-HL cells with TGF-beta1 at 1 ng/ml, a concentration that inhibited proliferation, enhanced presentation of Ag internalized via surface IgM (sIgM), but not vi...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200324018

    authors: Arai C,Ichijo T,Tanaka Y,Okada Y,Umeda M,Uchida T,Kiniwa M,Kakiuchi T

    更新日期:2003-07-01 00:00:00

  • B cells latently infected with murine gammaherpesvirus 68 (MHV-68) are present in the mouse thymus-A step toward immune evasion?

    abstract::We show that latently gammaherpesvirus-infected B cells are present in the thymus. This could result in a functional T-cell tolerance against certain viral epitopes. It is conceivable that also antigens from other viruses or pathogens may be conveyed to the thymus for their immune evasion. ...

    journal_title:European journal of immunology

    pub_type: 信件

    doi:10.1002/eji.201847886

    authors: Yamano T,Steinert M,Steer B,Klein L,Hammerschmidt W,Adler H

    更新日期:2019-02-01 00:00:00

  • MAL, a novel integral membrane protein of human T lymphocytes, associates with glycosylphosphatidylinositol-anchored proteins and Src-like tyrosine kinases.

    abstract::A large fraction of glycosylphosphatidylinositol (GPI)-anchored proteins and Src-like kinases are confined to glycolipid-enriched membrane (GEM) microdomains. The particular membrane topology of GPI-anchored proteins has led to the postulation of the existence of integral membrane proteins linking extracellular stimul...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1521-4141(199811)28:11<3675::AID-IMM

    authors: Millán J,Alonso MA

    更新日期:1998-11-01 00:00:00

  • Triggering of the alternative pathway of human T cell activation involves members of the T 200 family of glycoproteins.

    abstract::The present report describes functional characteristics of a monoclonal antibody (mAb), TA/211, which is reactive with a common determinant of the human CD45 molecules. When added to purified testing human T cells, TA/211, which is itself not mitogenic, leads to a strong proliferative response in combination with subm...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830190226

    authors: Schraven B,Roux M,Hutmacher B,Meuer SC

    更新日期:1989-02-01 00:00:00

  • Lymphocyte specificity to protein antigens. III. Capacity of low responder mice to beef cytochrome c to respond to a peptide fragment of the molecule.

    abstract::Lymph node cells derived from A.TH or A.TL mice primed with beef cytochrome c show striking patterns of reactivity when assayed in vitro for antigen-induced T cell proliferation. Whereas cells from A.TH mice respond specifically to beef cytochrome c or peptides composed of amino acids 1-65 and 81-104, cells from A.TL ...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830110210

    authors: Corradin G,Chiller JM

    更新日期:1981-02-01 00:00:00

  • Selective stimulation of murine cytotoxic T cell and antibody responses by particulate or monomeric hepatitis B virus surface (S) antigen.

    abstract::In the murine system, we tested in vivo the immunogenicity of different preparations of the yeast-derived surface antigen (S-antigen or S-protein) of hepatitis B virus (HBV). Native S-protein molecules self-assemble into stable 22-nm particles. BALB/c mice immunized with low doses of native S-particles without adjuvan...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830240512

    authors: Schirmbeck R,Melber K,Mertens T,Reimann J

    更新日期:1994-05-01 00:00:00

  • Commensal microflora and interferon-gamma promote steady-state interleukin-7 production in vivo.

    abstract::IL-7 is a major regulator of lymphocyte homeostasis; however, little is known about the mechanisms that regulate IL-7 production. To study Il7 gene regulation in vivo, we generated a novel IL-7-reporter mouse, which allows the non-invasive quantification of Il7 gene activity in live mice and, additionally, the simulta...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201040441

    authors: Shalapour S,Deiser K,Sercan O,Tuckermann J,Minnich K,Willimsky G,Blankenstein T,Hämmerling GJ,Arnold B,Schüler T

    更新日期:2010-09-01 00:00:00

  • B cells and CD22 are dispensable for the immediate antiinflammatory activity of intravenous immunoglobulins in vivo.

    abstract::Intravenous immunoglobulins (IVIgs) efficiently suppress a variety of autoimmune diseases. Over the past few years several potential mechanisms underlying this antiinflammatory activity have become apparent. Among these, terminal sialic acid residues in the sugar moiety of the immunoglobulin G constant fragment have b...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201242710

    authors: Schwab I,Seeling M,Biburger M,Aschermann S,Nitschke L,Nimmerjahn F

    更新日期:2012-12-01 00:00:00

  • CD2 mediates activation of the IFN-gamma intronic STAT binding region in mucosal T cells.

    abstract::The pathways leading to activation of mucosal lamina propria (LP) T cells differ from those of peripheral T cells. LP T cells exhibit enhanced IFN-gamma secretion when activated through the CD2 pathway. This study demonstrates CD2 signaling is followed by activation of STAT proteins in both peripheral blood mononuclea...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200322875

    authors: Gonsky R,Deem RL,Young HA,Targan SR

    更新日期:2003-05-01 00:00:00

  • Rapid IgE desensitization is antigen specific and impairs early and late mast cell responses targeting FcεRI internalization.

    abstract::Rapid IgE desensitization provides temporary tolerization for patients who have presented severe hypersensitivity reactions to food and drugs, protecting them from anaphylaxis, but the underlying mechanisms are still incompletely understood. Thus, here we develop an effective and reproducible in vitro model of rapid I...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201040810

    authors: Sancho-Serra Mdel C,Simarro M,Castells M

    更新日期:2011-04-01 00:00:00

  • CD2 is involved in regulating cyclic AMP levels in T cells.

    abstract::The human T lymphocyte-specific CD2 (T11) molecule, that regulates T cell activation, is capable of mediating increases of intracellular cAMP. Monoclonal antibodies directed to different epitopes of the CD2 molecule which either induce or inhibit T cell proliferation are capable of triggering an increase of cAMP compa...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830180620

    authors: Carrera AC,Rincón M,De Landázuri MO,López-Botet M

    更新日期:1988-06-01 00:00:00

  • The effect of carrier and carrier priming on the kinetics and pattern of somatic mutation in the V chi Ox1 gene.

    abstract::Priming mice with a chicken gamma globulin (CGG) carrier protein significantly accelerated the onset of somatic mutation in the V chi Ox1 gene when the mice were subsequently immunized with 2-phenyl-5-oxazolone (phOx) coupled to CGG. The first mutations were already detected 7 days after immunization, while in the tru...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830250834

    authors: Källberg E,Gray D,Leanderson T

    更新日期:1995-08-01 00:00:00

  • Multiple H-2 and non-H-2 genes controlling the antilysozyme response: alternative gene constellations can lead to responsiveness.

    abstract::Mice carrying the H-2b and H-2s haplotypes are genetically nonresponsive to hen egg-white lysozyme (HEL). Analysis of the anti-HEL response patterns of F1, F2 and backcross progeny showed that responsiveness was dominant and H-2 linked. From plaque-forming cell and serum assays in intra-H-2 recombinant mice, it was es...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830100513

    authors: Hill SW,Kipp DE,Melchers I,Frelinger JA,Sercarz EE

    更新日期:1980-05-01 00:00:00

  • Efficient protection against Mycobacterium tuberculosis by vaccination with a single subdominant epitope from the ESAT-6 antigen.

    abstract::We have investigated the vaccine potential of two peptides derived from the 6-kDa early secretory antigenic target (ESAT)-6 antigen in the mouse model of tuberculosis. The peptides were both strongly immunogenic in B6CBAF1 (H-2b,k) mice and primed recall responses of the same intensity after immunization. However, bot...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/1521-4141(200006)30:6<1724::AID-IMMU1724>3

    authors: Olsen AW,Hansen PR,Holm A,Andersen P

    更新日期:2000-06-01 00:00:00

  • A recombinant virus-like particle system derived from parvovirus as an efficient antigen carrier to elicit a polarized Th1 immune response without adjuvant.

    abstract::Hybrid virus-like particles (VLP) were prepared by self-assembly of the modified porcine parvovirus (PPV) VP2 capsid protein carrying a CD8+ or CD4+ T cell epitope. Immunization of mice with a single dose of these hybrid pseudo-particles, without adjuvant, induced strong cytotoxic T lymphocyte and T helper (Th) respon...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1521-4141(199804)28:04<1401::AID-IMM

    authors: Lo-Man R,Rueda P,Sedlik C,Deriaud E,Casal I,Leclerc C

    更新日期:1998-04-01 00:00:00

  • Negative regulation of autoreactive B cells in transgenic mice expressing a human pathogenic cold agglutinin.

    abstract::Cold agglutinins (CA) are autoantibodies that bind to erythrocyte carbohydrates at low temperatures and induce complement-mediated cell lysis, thus causing hemolytic anemia. Tolerance mechanisms towards CA-expressing B cells and the factors inducing pathogenic CA production are unknown. In order to develop an animal m...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/1521-4141(2000)30:8<2290::AID-IMMU2290>3.0

    authors: Havouis S,Dumas G,Avé P,Pritsch O,Huerre M,Dighiero G,Pourcel C

    更新日期:2000-08-01 00:00:00

  • Arginine depletion as a mechanism for the immune privilege of corneal allografts.

    abstract::The cornea is an immune privileged tissue. Since arginase has been found to modulate T-cell function by depleting arginine, we investigated the expression of arginase in the cornea and its possible role in immune privilege using a murine transplant model. We found that both the endothelium and epithelium of murine cor...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201141683

    authors: Fu H,Khan A,Coe D,Zaher S,Chai JG,Kropf P,Müller I,Larkin DF,George AJ

    更新日期:2011-10-01 00:00:00

  • Surface expression by mononuclear phagocytes of an epitope shared with mycobacterial heat shock protein 60.

    abstract::Bone marrow-derived macrophages were stained with a variety of monoclonal antibodies (mAb) with specificity for the mycobacterial heat shock protein (hsp) 60 or with specific rabbit antiserum raised against mycobacterial hsp 60. The mAb ML30 as well as the specific antiserum brightly stained bone marrow-derived macrop...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830210437

    authors: Wand-Württenberger A,Schoel B,Ivanyi J,Kaufmann SH

    更新日期:1991-04-01 00:00:00

  • The domestic pig as human-relevant large animal model to study adaptive antifungal immune responses against airborne Aspergillus fumigatus.

    abstract::Pulmonary mucosal immune response is critical for preventing opportunistic Aspergillus fumigatus infections. Although fungus-specific CD4+ T cells in blood are described to reflect the actual host-pathogen interaction status, little is known about Aspergillus-specific pulmonary T-cell responses. Here, we exploit the d...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201948524

    authors: Schmidt S,Ebner F,Rosen K,Kniemeyer O,Brakhage AA,Löffler J,Seif M,Springer J,Schlosser J,Scharek-Tedin L,Scheffold A,Bacher P,Kühl AA,Rösler U,Hartmann S

    更新日期:2020-11-01 00:00:00

  • Immunization through dermal delivery of protein-encoding DNA: a role for migratory dendritic cells.

    abstract::The early mechanisms by which DNA-dependent immunization occurs remain poorly understood. We determined whether intradermal injection of a cytomegalovirus (CMV) promoter-driven plasmid encoding hen egg lysozyme (pCMV:HEL) induced sensitization against the encoded protein, and whether cutaneous dendritic cells (DC) wer...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1521-4141(199902)29:02<446::AID-IMMU

    authors: Bouloc A,Walker P,Grivel JC,Vogel JC,Katz SI

    更新日期:1999-02-01 00:00:00

  • Interferon-alpha can synergize with interleukin 2 for human in vitro antibody response.

    abstract::We tested the effect of interferon (IFN)-alpha on the specific anti-2,4,6-trinitrophenyl (TNP) response induced by TNP-conjugated polyacrylamide beads in cultures of purified human B cells. IFN-alpha alone had no effect. Interleukin 2 (IL2) alone had a restorative effect which could be considerably (10X) enhanced by I...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830180912

    authors: Delfraissy JF,Wallon C,Galanaud P

    更新日期:1988-09-01 00:00:00

  • MyD88 and TRIF synergistic interaction is required for TH1-cell polarization with a synthetic TLR4 agonist adjuvant.

    abstract::Glucopyranosyl lipid adjuvant-stable emulsion (GLA-SE) is a synthetic adjuvant TLR4 agonist that promotes potent poly-functional T(H)1 responses. Different TLR4 agonists may preferentially signal via MyD88 or TIR-domain-containing adapter inducing IFN-beta (TRIF) to exert adjuvant effects; however, the contribution of...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201243124

    authors: Orr MT,Duthie MS,Windish HP,Lucas EA,Guderian JA,Hudson TE,Shaverdian N,O'Donnell J,Desbien AL,Reed SG,Coler RN

    更新日期:2013-09-01 00:00:00

  • Heterogeneity in the molecular defect leading to the leukocyte adhesion deficiency.

    abstract::Leukocyte adhesion deficiency (LAD) is a recessive autosomal disease characterized by life-threatening recurrent bacterial infections, by defective functions of leukocytes and by deficient membrane expression of leukocyte adhesion glycoproteins. These proteins, LFA-1, Mac-1 and p150,95, are alpha 1/beta 1 heterodimers...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830181016

    authors: Dimanche-Boitrel MT,Guyot A,De Saint-Basile G,Fischer A,Griscelli C,Lisowska-Grospierre B

    更新日期:1988-10-01 00:00:00