Abstract:
:Innate immunity, which is unable to discriminate self from allo-antigens, is thought to be important players in the induction of miscarriages. Here, we show that the administration of IL-12 to syngeneic-mated C57BL/6 mice on gestation day 7.5 (Gd 7.5), drives significant miscarriages in pregnant females. Furthermore, the administration on Gd 7.5 of α-galactosylceramide (α-GalCer), which is known to activate invariant natural killer T (iNKT) cells, induced miscarriages in both syngeneic-mated C57BL/6 mice and allogeneic-mated mice (C57BL/6 (♀) × BALB/c (♂)). Surprisingly, the percentages of both DEC-205(+) DCs and CD1d-restricted NK1.1(+) iNKT cells were higher in the myometrium of pregnant mice treated i.p. with α-GalCer than in the decidua. IL-12 secreted from α-GalCer-activated DEC-205(+) DCs stimulated the secretion of cytokines, including IL-2, IL-4, IFN-γ, TNF-α, perforin, and granzyme B, from the NK1.1(+) iNKT cells in the myometrium, leading to fetal loss in pregnant mice. Finally, the i.p. administration of IL-12 and/or α-GalCer in iNKT-deficient Jα18(-/-) (Jα18 KO) mice did not induce miscarriages. This study provides a new perspective on the importance of the myometrium, rather than the decidua, in regulating pregnancy and a mechanism of miscarriage mediated by activated DEC-205(+) DCs and NK1.1(+) iNKT cells in the myometrium of pregnant mice.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Ichikawa T,Negishi Y,Shimizu M,Takeshita T,Takahashi Hdoi
10.1002/eji.201545923subject
Has Abstractpub_date
2016-08-01 00:00:00pages
1867-77issue
8eissn
0014-2980issn
1521-4141journal_volume
46pub_type
杂志文章abstract::CD4+ helper T lymphocytes and CD8+ killer T lymphocytes are both generated in the thymus from common precursor cells expressing CD4 and CD8. The development of immature CD4 CD8+ thymocytes into mature 'single-positive' T cells requires T cell antigen-receptor (TCR)-mediated positive selection signals. Although it is k...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271231
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abstract::It is widely believed that IL-4 exerts its influence by profiling the immune response during priming and expansion of immune cells, and thereby modulates the outcome of chronic inflammation. In the present investigation, collagen antibody-induced arthritis (CAIA) was used to delineate the role of IL-4 in a T cell-inde...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535633
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240950
更新日期:1994-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221219
更新日期:1992-12-01 00:00:00
abstract::Natural killer T (NKT) lymphocytes rapidly produce several cytokines, including IL-4 and IFN-gamma, upon activation, and act as regulatory cells at an early interphase of innate and adaptive immune responses. They have been implicated as important elements in diverse immune responses including the regulation of autoim...
journal_title:European journal of immunology
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abstract::The pathways leading to activation of mucosal lamina propria (LP) T cells differ from those of peripheral T cells. LP T cells exhibit enhanced IFN-gamma secretion when activated through the CD2 pathway. This study demonstrates CD2 signaling is followed by activation of STAT proteins in both peripheral blood mononuclea...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200322875
更新日期:2003-05-01 00:00:00
abstract::It is widely believed that DC, but not macrophages, prime naïve T cells in vivo. Here, we investigated the ability of CD68-expressing cells (commonly defined as macrophages) in priming autoreactive T cells and initiating collagen-induced arthritis (CIA) in the mouse. For this purpose, a transgenic mouse was developed ...
journal_title:European journal of immunology
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abstract::The immune system relies on homeostatic mechanisms in order to adapt to the changing requirements encountered during steady-state existence and activation by antigen. For T cells, this involves maintenance of a diverse repertoire of naïve cells, rapid elimination of effector cells after pathogen clearance, and long-te...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
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更新日期:2009-08-01 00:00:00
abstract::A panel of closely and distantly related lysozymes and lysozyme-peptide fragments were utilized in assessing the specificity repertoire of murine anti-hen egg white lysozyme hybridomas. The 44 monoclonal antibodies could be divided into a minimum of 18 fine specificity groups in tests using the lysozyme panel. Two hyb...
journal_title:European journal of immunology
pub_type: 杂志文章
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abstract::The CD11c(+) cell population in the non-parenchymal cell population of the mouse liver contains dendritic cells (DC), NK cells, B cells and T cells. In the hepatic CD11c(+) DC population from immunocompetent or immunodeficient [recombinase-activating gene-1 (RAG1)(-/-)] C57BL/6 mice (rigorously depleted of T cells, B ...
journal_title:European journal of immunology
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abstract::This Viewpoint series provides authoritative and detailed outlines of exciting areas of DC research. Some of the subjects that frequently come up include development of DC; distribution of DC in lymphoid and non-lymphoid tissues such as skin, intestine and lung; different forms or subsets of DC; and the role of DC in ...
journal_title:European journal of immunology
pub_type:
doi:10.1002/eji.201040733
更新日期:2010-08-01 00:00:00
abstract::The immune system has developed several regulatory mechanisms to maintain homeostasis of adaptive immune responses. T-cell programmed death (PD)-1 recognition of B7-H1 (PD-L1) expressed on APC and non-lymphoid tissue regulates T-cell activation. We show that B7-H1(-/-) mice exhibit exacerbated proteoglycan (PG)-induce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040690
更新日期:2010-11-01 00:00:00
abstract::Wiskott-Aldrich syndrome (WAS) is an X-linked disorder characterized by congenital thrombocytopenia and progressive deterioration of the immune function. Dendritic cells (DC) are key effectors in the induction of specific immunity and are highly specialized in antigen uptake and subsequent migration to draining lymph ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200112)31:12<3413::aid-immu3413>
更新日期:2001-12-01 00:00:00
abstract::A rapidly-growing, HAT-sensitive cell line LICR-LON-HMy2 has been derived from the ARH-77 human plasma cell leukemia-derived line. It lacks the enzyme hypoxanthine phosphoribosyltransferase (EC 2.4.2.8). Hybrids have been reproducibly made for more than a year and in independent laboratories with lymphocytes from tons...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830120804
更新日期:1982-08-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261251
更新日期:1996-12-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201041141
更新日期:2011-05-01 00:00:00
abstract::The influence of Th2 cytokines in tuberculosis has been a matter of dispute. Here we report that IL-4 has a profound regulatory effect on the infection of BALB/c mice with Mycobacterium tuberculosis. Depletion of IL-4 with a neutralizing mAb caused only evanescent reduction of lung infection, but when combined with i....
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636764
更新日期:2007-03-01 00:00:00
abstract::Bcl-3 is an atypical member of the IκB family. Bcl-3 functions as a cofactor of p50/NF-κB1 or p52/NF-κB2 homodimers in nuclei, where it modulates NF-κB-regulated transcription in a context-dependent way. Bcl-3 has tumorigenic potential, is critical in host defense of pathogens, and has been reported to ameliorate or e...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201444994
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abstract::Dendritic cells (DC) are extremely proficient inducers of naïve CD4+ T cell activation due to their high expression level of peptide-MHC and an array of accessory molecules involved in cell migration, adhesion and co-signaling, including PD-1 ligand 1 (PD-L1) and PD-1 ligand 2 (PD-L2). Whether PD-L1 and PD-L2 have a s...
journal_title:European journal of immunology
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doi:10.1002/eji.200635978
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abstract::Protein antigens with both allergenic and immunoprotective properties represent appropriate molecules to study IgE and IgG regulation. We have established a panel of T cell clones specific to bee venom phospholipase A2 (PLA) from human individuals allergic, hyposensitized or immune (protected) to bee sting. All clones...
journal_title:European journal of immunology
pub_type: 杂志文章
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200007)30:7<2007::AID-IMMU2007>3
更新日期:2000-07-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636421
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210721
更新日期:1991-07-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060112
更新日期:1976-01-01 00:00:00
abstract::To investigate negative selection events during intrathymic ontogeny, we established T cell receptor (TCR)-transgenic mice [N15tg/RAG-2-/- (H-2b)] expressing a single TCR specific for vesicular stomatitis virus nuclear octapeptide N52-59 (VSV8) in the context of the major histocompatibility complex (MHC) class I molec...
journal_title:European journal of immunology
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doi:10.1002/eji.1830270923
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journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1991-05-01 00:00:00
abstract::We investigated here the potential role of Toll-like receptors (TLR) and the adaptor protein MyD88 in innate immunity responses to Cryptococcus neoformans, a pathogenic encapsulated yeast. Peritoneal macrophages from MyD88(-/-) or TLR2(-/-) mice released significantly less TNF-alpha, compared with wild-type controls, ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425799
更新日期:2005-03-01 00:00:00
abstract::B14-2-14 is a monoclonal cytotoxic IgM antibody which reacts with thymocytes of all mouse strains tested. The fraction of positive cells (by visual immunofluorescence) varies between strains from about 25-45% in A.CA to 65-85% in C57BL/6, and high levels are dominant in F1 hybrids. In the periphery, the antigen is fou...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130610
更新日期:1983-06-01 00:00:00