Effects of interferon-alpha on cytokine profile, T cell receptor repertoire and peptide reactivity of human allergen-specific T cells.


:A large panel of T cell clones (TCC) specific for the recombinant form of Poa pratensis allergen (rKBG7.2 or Poa p9) were established from the peripheral blood of grass pollen-sensitive donor in the absence or presence of recombinant interferon-alpha (IFN-alpha) in bulk culture and their pattern of cytokine secretion, peptide reactivity and TCR V beta repertoire was examined. The majority of allergen-specific TCC derived in absence of IFN-alpha produced high amounts of interleukin-4 (IL-4) and IL-5 but not IFN-gamma (Th2 cells), while most of TCC derived in presence of IFN-alpha produced IFN-gamma but not, or limited amounts of, IL-4 and IL-5 (Th1 or Th0 cells). Of 24 TCC established in the presence of IFN-alpha, 22 were able to recognize a single allergen peptide, p26, while none of the clones established in the absence of IFN-alpha showed a similar specificity. The majority of both clones expressed the V beta 2 element regardless of whether they were established in the presence of INF-alpha, but the presence of IFN-alpha favored the expansion of V beta 2+, V beta 17+ and V beta 22+ Poa p9-specific T cells, whereas in the absence of IFN-alpha, other TCR V beta-bearing T cells (V beta 5, and V beta 6.7, and V beta 14) were expanded in addition to V beta 2+ T cells. None of V beta 2+ clones established in the absence of IFN-alpha reacted with p26, whereas all the V beta 2+ clones established in its presence in the absence of interferon-alpha reacted with p26, whereas all the V beta 2+ clones established in its presence reacted to this peptide. IFN-alpha also shifted the TCR V beta repertoire of both Poa p9- and Lolium perenne group 1 (Lol p1)-specific T cell lines generated from the same patient and from a different grass-sensitive individual. These data demonstrate that IFN-alpha modulates the development of allergen-specific T cells in vitro, and suggest that IFN-alpha may represent a useful tool for novel immunotherapeutic approaches in allergic disorders.


Eur J Immunol


Parronchi P,Mohapatra S,Sampognaro S,Giannarini L,Wahn U,Chong P,Mohapatra S,Maggi E,Renz H,Romagnani S




Has Abstract


1996-03-01 00:00:00












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    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Castro MA,Tavares PA,Almeida MS,Nunes RJ,Wright MD,Mason D,Moreira A,Carmo AM

    更新日期:2002-05-01 00:00:00

  • Implications of genome-wide association studies in novel therapeutics in primary biliary cirrhosis.

    abstract::Genome-wide association studies (GWAS) have revolutionized the search for genetic influences on complex disorders, such as primary biliary cirrhosis (PBC). Recent GWAS have identified many disease-associated genetic variants. These, overall, highlighted the remarkable contribution of key immunological pathways in PBC ...

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    pub_type: 杂志文章,评审


    authors: Carbone M,Lleo A,Sandford RN,Invernizzi P

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    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Jaehn PS,Zaenker KS,Schmitz J,Dzionek A

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    journal_title:European journal of immunology

    pub_type: 杂志文章


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    更新日期:2003-11-01 00:00:00

  • Receptors for immune complexes on cells within a polyoma virus-induced murine sarcoma.

    abstract::Cells present in the polyoma virus-induced murine ascites tumor SEYF-a showed the capacity to fix soluble immune complexes of ovalbumin anti-ovalbumin. The fixation could be inhibited by preincubating the cells with antisera directed against H-2 antigens and with syngeneic anti-tumor antibodies. The latter did not rea...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Braslawsky GR,Serban D,Witz IP

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    journal_title:European journal of immunology

    pub_type: 杂志文章


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    journal_title:European journal of immunology

    pub_type: 杂志文章


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  • Peptides related to the antigenic determinant block T cell recognition of the native protein as processed by antigen-presenting cells.

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    journal_title:European journal of immunology

    pub_type: 杂志文章


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    journal_title:European journal of immunology

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    authors: Chen X,Niyonsaba F,Ushio H,Hara M,Yokoi H,Matsumoto K,Saito H,Nagaoka I,Ikeda S,Okumura K,Ogawa H

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  • Environmental and allele-specific influences on T cell receptor gene rearrangement: skewed alpha, delta, and gamma gene rearrangement patterns in chimeric mice.

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    journal_title:European journal of immunology

    pub_type: 杂志文章


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    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Volkmann A,Neefjes J,Stockinger B

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    journal_title:European journal of immunology

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  • Rapid IgE desensitization is antigen specific and impairs early and late mast cell responses targeting FcεRI internalization.

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    journal_title:European journal of immunology

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    journal_title:European journal of immunology

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    authors: Zaghouani H,Kuzu Y,Kuzu H,Brumeanu TD,Swiggard WJ,Steinman RM,Bona CA

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    journal_title:European journal of immunology

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    authors: Teng YT,Gorczynski RM,Iwasaki S,Williams DB,Hozumi N

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    authors: Kato A,Takaori-Kondo A,Minato N,Hamazaki Y

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    journal_title:European journal of immunology

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    authors: Panzer U,Uguccioni M

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  • Urtica dioica agglutinin, a V beta 8.3-specific superantigen, prevents the development of the systemic lupus erythematosus-like pathology of MRL lpr/lpr mice.

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    journal_title:European journal of immunology

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    authors: Musette P,Galelli A,Chabre H,Callard P,Peumans W,Truffa-Bachi P,Kourilsky P,Gachelin G

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  • A synthetic HIV-1 Tat protein breaches the skin barrier and elicits Tat-neutralizing antibodies and cellular immunity.

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    journal_title:European journal of immunology

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    journal_title:European journal of immunology

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    journal_title:European journal of immunology

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    authors: Tuscano JM,Engel P,Tedder TF,Agarwal A,Kehrl JH

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    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: von Boehmer H,Fathman CG,Haas W

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    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Bluestone JA,Auchincloss H Jr,Sachs DH,Fibi M,Hämmerling GJ

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    journal_title:European journal of immunology

    pub_type: 杂志文章


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    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Flutter B,Nestle FO

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    journal_title:European journal of immunology

    pub_type: 杂志文章


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    journal_title:European journal of immunology

    pub_type: 杂志文章


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    更新日期:2002-04-01 00:00:00

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    journal_title:European journal of immunology

    pub_type: 杂志文章


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    更新日期:1988-01-01 00:00:00

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    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Ruedl C,Hubele S

    更新日期:1997-06-01 00:00:00