Abstract:
:A large panel of T cell clones (TCC) specific for the recombinant form of Poa pratensis allergen (rKBG7.2 or Poa p9) were established from the peripheral blood of grass pollen-sensitive donor in the absence or presence of recombinant interferon-alpha (IFN-alpha) in bulk culture and their pattern of cytokine secretion, peptide reactivity and TCR V beta repertoire was examined. The majority of allergen-specific TCC derived in absence of IFN-alpha produced high amounts of interleukin-4 (IL-4) and IL-5 but not IFN-gamma (Th2 cells), while most of TCC derived in presence of IFN-alpha produced IFN-gamma but not, or limited amounts of, IL-4 and IL-5 (Th1 or Th0 cells). Of 24 TCC established in the presence of IFN-alpha, 22 were able to recognize a single allergen peptide, p26, while none of the clones established in the absence of IFN-alpha showed a similar specificity. The majority of both clones expressed the V beta 2 element regardless of whether they were established in the presence of INF-alpha, but the presence of IFN-alpha favored the expansion of V beta 2+, V beta 17+ and V beta 22+ Poa p9-specific T cells, whereas in the absence of IFN-alpha, other TCR V beta-bearing T cells (V beta 5, and V beta 6.7, and V beta 14) were expanded in addition to V beta 2+ T cells. None of V beta 2+ clones established in the absence of IFN-alpha reacted with p26, whereas all the V beta 2+ clones established in its presence in the absence of interferon-alpha reacted with p26, whereas all the V beta 2+ clones established in its presence reacted to this peptide. IFN-alpha also shifted the TCR V beta repertoire of both Poa p9- and Lolium perenne group 1 (Lol p1)-specific T cell lines generated from the same patient and from a different grass-sensitive individual. These data demonstrate that IFN-alpha modulates the development of allergen-specific T cells in vitro, and suggest that IFN-alpha may represent a useful tool for novel immunotherapeutic approaches in allergic disorders.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Parronchi P,Mohapatra S,Sampognaro S,Giannarini L,Wahn U,Chong P,Mohapatra S,Maggi E,Renz H,Romagnani Sdoi
10.1002/eji.1830260328subject
Has Abstractpub_date
1996-03-01 00:00:00pages
697-703issue
3eissn
0014-2980issn
1521-4141journal_volume
26pub_type
杂志文章abstract::Development of Th1 and Th2 effector lymphocytes is driven primarily by IL-12 or IL-4, but is also influenced by the strength of antigenic stimulation. However, the mechanism by which TCR signaling contributes to T cell polarization remains elusive. We show that in the presence of IL-12 a short TCR stimulation can lead...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199912)29:12<4092::AID-IMM
更新日期:1999-12-01 00:00:00
abstract::Endogenous superantigens (SAg) presented by MHC class II IA molecules induce slow-evolving negative selection of alpha beta T cells. The role of both B and gamma delta T cells on the regulation of these SAg-specific alpha beta T cell responses was addressed in IA(b+)IE(b-) C57BL/6 mice bearing genetically induced B ce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200109)31:9<2811::aid-immu2811>3
更新日期:2001-09-01 00:00:00
abstract::Induction of the glucocorticoid-induced leucine zipper (GILZ) by glucocorticoids plays a role in their antiinflammatory action, whereas GILZ expression is reduced under inflammatory conditions. The mechanisms regulating GILZ expression during inflammation, however, have not yet been characterized. Here, we investigate...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142081
更新日期:2012-05-01 00:00:00
abstract::Members of the innate lymphoid cell (ILC) family have been implicated in the development of thymic microenvironments and the recovery of this architecture after damage. However, a detailed characterization of this family in the thymus is lacking. To better understand the thymic ILC compartment, we have utilized multip...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201847511
更新日期:2018-09-01 00:00:00
abstract::In previous publications it was shown by avidity measurements, cross-reactivity patterns and genetic analyses, that the tetrapeptide T-T-G-G is the immuno-dominant epitope of the synthetic polypeptide (T, G)-A--L. In the present study this close immunological relationship between the random multichain copolymer (T, G)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060905
更新日期:1976-09-01 00:00:00
abstract::The induction of B cell tolerance to 2,4-dinitrophenyl conjugates of polysaccharide antigens (levan or dextran) was studied in mice primed with keyhole limpet hemocyanin (KLH) or 2,4,6-trinitrophenylated KLH. The relationship of the epitope density of the tolerogen with avidity of B cell receptors (as judged indirectl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060910
更新日期:1976-09-01 00:00:00
abstract::Delayed hypersensitivity (DH) reaction to human Ig, corynebacterium parvum or allogeneic cells at the site of tumor cell injection, suppressed the fibrosarcoma (SCFS) growth in chickens. Spleen cells of SC chickens sensitized to human Ig or C. parvum suppressed SCFS growth when adoptively transferred with tumor cells,...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830080408
更新日期:1978-04-01 00:00:00
abstract::Vaccine efficacy largely depends upon DC targeting and activation. The most potent TLR soluble ligands induce diffuse DC activation, which may be associated with marked pro-inflammatory responses and possibly adverse effects. This raises the concern that effective vaccine adjuvants may similarly rely on widespread DC ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737889
更新日期:2008-05-01 00:00:00
abstract::Several groups have described that a low percentage of in vitro cultured T cell receptor (TcR) gamma/delta cells express CD8. Contrary to TcR alpha/beta cells, however, CD8 on these TcR gamma/delta cells was shown to be a CD8 alpha homodimer. We describe here that addition of interleukin-7 (IL-7) to a short-term in vi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220902
更新日期:1992-09-01 00:00:00
abstract::Peptides associated with HLA-B35 commonly have a proline or occasionally a serine residue in the P2 anchor position of the peptide, with a tyrosine at the C terminus. Based on this motif, we identified an octamer epitope from influenza A matrix protein which is presented by HLA-B35. The requirements for MHC binding an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260210
更新日期:1996-02-01 00:00:00
abstract::Interleukin (IL)-4-transgenic mice were used as a model system to study the consequences of low levels of IL-4 expression for the expression of other cytokines examined by quantitative polymerase chain reaction (PCR). For this purpose, a plasmid was constructed which contains, in tandem array, 5' and 3' primer sequenc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220511
更新日期:1992-05-01 00:00:00
abstract::Sporozoite surface protein 2 has been identified as a target of malaria vaccines designed to produce protective CD8+ cytotoxic T lymphocytes (CTL) because mice immunized with mastocytoma cells expressing a fragment of Plasmodium yoelii sporozoite surface protein 2 (PySSP2) are protected against malaria by an immune re...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240705
更新日期:1994-07-01 00:00:00
abstract::The expression of the Pit-1/GHF-1 transcription factor (hereafter Pit-1), which controls the expression of growth hormone (GH) and prolactin (PRL) in the pituitary gland, has been documented in human and rat hemopoietic and lymphoid tissues and cell lines. Pit-1 mRNA was detected by in situ hybridization in about 1% o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230428
更新日期:1993-04-01 00:00:00
abstract::The ligand for the B cell surface antigen CD40 was recently cloned from a murine thymoma cDNA library and shown to be expressed on activated T cells. In this study, we investigate the biological effects of murine recombinant CD40 ligand. The recombinant CD40 ligand expressed on the CV-1/EBNA monkey fibroblast cell lin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230510
更新日期:1993-05-01 00:00:00
abstract::E-selectin is an endothelium-specific inducible adhesion molecule which binds several inflammatory cell types, including neutrophils, monocytes, natural killer cells and a subset of memory T cells. E-selectin is important in the initial rolling interaction of these cells on inflamed endothelium. The transient kinetics...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261221
更新日期:1996-12-01 00:00:00
abstract::This is the first report on the establishment of the readily identifiable and functioning thymic nurse cell (TNC) clones from the mouse thymus. In the course of the culture of an epithelial cell line using a medium with a low concentration of Ca2+ from the spontaneous BALB/c thymic tumor, lymphocytes as well as thymic...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180525
更新日期:1988-05-01 00:00:00
abstract::The activation requirements for antigen-dependent proliferation of CD4+ T cells are well documented, while the events leading to the inactivation phase are poorly understood. Here, we tested the hypothesis that the lymphocyte-activation gene 3 (LAG-3), a second major histocompatibility complex (MHC) class II ligand, p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830241246
更新日期:1994-12-01 00:00:00
abstract::The capacity of old (18-24 months) C57BL/6 mice to develop an immune reaction against MC-B6-1 fibrosarcoma cells was studied using in vivo adoptive transfer experiments (Winn assay) and in vitro T cell-mediated cytotoxicity test. Anti-tumor immunity was found to decline with age, as indicated by a decreased anti-tumor...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170302
更新日期:1987-03-01 00:00:00
abstract::Fourteen noncytotoxic human helper T cell clones were examined for autocrine proliferative responses and cytotoxicity to tumor cells after stimulation with 12-O-tetradecanoylphorbol 13-acetate (TPA) and ionomycin (Io). Although all clones responded to alloantigen, they could be divided into two groups based on their p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180729
更新日期:1988-07-01 00:00:00
abstract::An association between T-cell lymphopenia and autoimmunity has long been proposed, but it remains to be elucidated whether T-cell lymphopenia affects B-cell responses to autoantigens. Human neonatal thymectomy (Tx) results in a decrease in T-cell numbers and we used this model to study the development of autoreactivit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201746971
更新日期:2017-11-01 00:00:00
abstract::Mononuclear phagocytes and lymphocytes can both synthesize cytotoxins which kill certain tumor cell lines and recent evidence suggests that these cytotoxins may be identical. In this study, human monocyte cytotoxin and lymphoblastoid lymphotoxin have been critically compared in terms of specificity, physicochemical ch...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150321
更新日期:1985-03-01 00:00:00
abstract::Peptide ligands presented by MHC class I molecules are generated in a cascade of proteolytic events starting with the proteasome in the cytosol and frequently terminating with trimming aminopeptidases in the endoplasmic reticulum. Several cytosolic proteases can carry out intermediate proteolytic steps between these s...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.200838181
更新日期:2008-03-01 00:00:00
abstract::The murine monoclonal antibody F23.1 reacts with an allotypic determinant on the beta chain of the T cell receptor expressed by approximately 20% of T helper and cytotoxic T lymphocytes of most common mouse strains. This IgG2a antibody, either in soluble form or covalently coupled to Sepharose beads, can activate rest...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160310
更新日期:1986-03-01 00:00:00
abstract::Human antibodies against HIV-1 have been sought to study neutralization events on the molecular level, and for possible use in passive immune intervention. The development of phage display techniques has opened the possibility of rapidly generating human monoclonal antibodies with desired specificities. We and others ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261231
更新日期:1996-12-01 00:00:00
abstract::In this report, we demonstrate that gp40, a molecule previously shown to be expressed by thymic epithelial cell lines in vitro and by thymic epithelial cells in vivo, is the murine homolog of human Ep-CAM, a calcium-independent homotypic adhesion molecule. gp40 is also expressed at low levels by thymocytes and periphe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260220
更新日期:1996-02-01 00:00:00
abstract::B cell development is dependent on both direct interactions with stromal cells and their secreted cytokines. The precise mechanisms by which these interactions regulate B cell differentiation are currently unknown. We report here that a novel growth factor thymic stromal-derived lymphopoietin (TSLP) can replace the ac...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260103
更新日期:1996-01-01 00:00:00
abstract::Oxidant stress can initiate or enhance inflammatory responses during tissue injury, possibly through activation of redox-sensitive chemokines. Because the transcription factor Nrf2 (NF-E2-related factor 2) is responsive to oxidative stress, and induces expression of cytoprotective and antioxidant genes that attenuate ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200526116
更新日期:2005-11-01 00:00:00
abstract::Maintenance of homeostasis at body barriers that are constantly challenged by microbes, toxins and potentially bioactive (macro)molecules requires complex, highly orchestrated mechanisms of protection. Recent discoveries in respiratory research have shed light on the unprecedented role of airway epithelial cells (AEC)...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201847811
更新日期:2020-05-01 00:00:00
abstract::Human papillomavirus (HPV) infections account for more than 50% of infection-linked cancers in women worldwide. The immune system controls, at least partially, viral infection and around 90% of HPV-infected women clear the virus within two years. However, it remains unclear which immune cells are implicated in this pr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141693
更新日期:2011-11-01 00:00:00
abstract::Act1 is a negative regulator of B-cell activation factor of the TNF family (BAFF) and CD40L-induced signaling. BALB/C mice lacking Act1 develop systemic autoimmunity resembling systemic lupus erythematosus (SLE) and Sjögren's syndrome (SjS). SLE and SjS are characterized by anti-nuclear IgG autoantibody (ANA-IgG) prod...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142238
更新日期:2012-07-01 00:00:00