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Deficient tumor-specific immunity in old mice: in vivo mediation by suppressor cells, and correction of the defect by interleukin 2 supplementation in vitro but not in vivo.

Abstract：

:The capacity of old (18-24 months) C57BL/6 mice to develop an immune reaction against MC-B6-1 fibrosarcoma cells was studied using in vivo adoptive transfer experiments (Winn assay) and in vitro T cell-mediated cytotoxicity test. Anti-tumor immunity was found to decline with age, as indicated by a decreased anti-tumor growth T cell activity. A suppressive activity was also found present in the splenic T cell population of old mice which can inhibit the in vivo generation of immune T cells in young mice. These suppressors, or their precursors, were resistant to cyclophosphamide treatment and were effective only when administered 3 days before the immunization of young mice. These mice developed immune T cells perfectly when the suppressors were administered 3 days after immunization, indicating that suppressors may act at an early phase of T cell activation. The protective activity of T cells in vivo correlated well with the in vitro T cell cytotoxicity for MC-B6-1 tumor cells, as both were depressed in old mice. Exogenous interleukin 2 (IL 2) addition during the 4-day culture period partially restored the low cytotoxic activity of old immunized lymphocytes, suggesting that specific clones were present but that a lack of IL 2 limited their expansion. However, in vivo supplementation with IL 2 administered after immunization did not increase the protection mediated by old immunized T cells but, rather, increased the suppression. This work demonstrates the presence of a T cell suppressive activity in the spleen of old mice but also indicates that precursors of cytotoxic cells are generated by the immunization. It seems that in vitro IL 2 addition increases cytotoxic cells while in vivo IL 2 administration amplifies the development of suppressor cells generated during immunization of aged mice.

journal_name

Eur J Immunol

journal_title

European journal of immunology

authors

Bruley-Rosset M,Payelle B

doi

10.1002/eji.1830170302

subject

Has Abstract

pub_date

1987-03-01 00:00:00

pages

307-12

issue

eissn

0014-2980

issn

1521-4141

journal_volume

pub_type

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Deficient tumor-specific immunity in old mice: in vivo mediation by suppressor cells, and correction of the defect by interleukin 2 supplementation in vitro but not in vivo.