Abstract:
:The early mechanisms by which DNA-dependent immunization occurs remain poorly understood. We determined whether intradermal injection of a cytomegalovirus (CMV) promoter-driven plasmid encoding hen egg lysozyme (pCMV:HEL) induced sensitization against the encoded protein, and whether cutaneous dendritic cells (DC) were involved in this sensitization. Both humoral and cellular responses to HEL were observed. DC that migrated from skin explant culture 3 days after injection of pCMV:HEL DNA contained mRNA encoding HEL. They induced a 3.5-7-fold increase in [3H]thymidine incorporation by HEL protein-primed CD4+ T cells compared to that induced by DC from mice injected with control plasmid. DC emigrating from skin explants recovered from pCMV:HEL injected mice also sensitized naive mice after adoptive transfer and induced the generation of CTL. Thus following DNA delivery within the dermis, DC can induce primary and secondary immune responses.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Bouloc A,Walker P,Grivel JC,Vogel JC,Katz SIdoi
10.1002/(SICI)1521-4141(199902)29:02<446::AID-IMMUkeywords:
subject
Has Abstractpub_date
1999-02-01 00:00:00pages
446-54issue
2eissn
0014-2980issn
1521-4141journal_volume
29pub_type
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