Abstract:
:Tumor necrosis factor (TNF) is one of the key primary response genes in the immune system that can be activated by a variety of stimuli. Previous analysis of chromatin accessibility to DNaseI demonstrated open chromatin conformation of the TNF proximal promoter in T cells. Here, using chromatin probing with restriction enzyme EcoNI and micrococcal nuclease we show that in contrast to the proximal promoter, the TNF transcription start site remains in a closed chromatin configuration in primary T helper (Th) cells, but acquires an open state after activation or polarization under Th1 and Th17 conditions. We further demonstrate that transcription factor c-Jun plays a pivotal role in the maintenance of open chromatin conformation at the transcription start site of the TNF gene.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Shebzukhov YV,Horn K,Brazhnik KI,Drutskaya MS,Kuchmiy AA,Kuprash DV,Nedospasov SAdoi
10.1002/eji.201243297subject
Has Abstractpub_date
2014-01-01 00:00:00pages
251-64issue
1eissn
0014-2980issn
1521-4141journal_volume
44pub_type
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