Abstract:
:The interaction of KB-V1, a multidrug resistant (MDR) variant of the KB-3-1 human oral carcinoma, with human complement was investigated. KB-V1 cells were found to be more sensitive than KB-3-1 cells to complement-mediated lysis. Detailed analysis of the capacity of KB cells to activate human complement demonstrated that both C3b deposition and formation of the membrane attack complex (MAC) are higher on KB-V1 than on KB-3-1 cells. Furthermore, the MAC formed on KB-V1 cells, but not on KB-3-1 cells, was found to be resistant to trypsin treatment, i.e. more stably inserted into the plasma membrane. Immunofluorescence analysis by flow cytometry showed that KB-V1 cells express less decay-accelerating factor (DAF, CD55) than KB-3-1 cells. Two other complement regulatory proteins, membrane cofactor protein (MCP, CD46) and CD59 are expressed to a similar extent on both KB-V1 and KB-3-1 cells. Treatment of KB-V1 cells with neutralizing anti-P-glycoprotein (P-gp) monoclonal antibodies reduced their sensitivity to complement. In addition, KB-V1 revertants which cease to express P-gp become more resistant to complement. These results indicate that multiple factors, such as reduced expression of DAF, enhanced deposition of C3b and increased binding and stability of the MAC may contribute to the increased complement sensitivity of KB-V1 cells. It is suggested that P-gp is responsible for the complement-sensitive phenotype of KB-V1 cells.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Bomstein Y,Fishelson Zdoi
10.1002/eji.1830270913subject
Has Abstractpub_date
1997-09-01 00:00:00pages
2204-11issue
9eissn
0014-2980issn
1521-4141journal_volume
27pub_type
杂志文章abstract::Act1 is a negative regulator of B-cell activation factor of the TNF family (BAFF) and CD40L-induced signaling. BALB/C mice lacking Act1 develop systemic autoimmunity resembling systemic lupus erythematosus (SLE) and Sjögren's syndrome (SjS). SLE and SjS are characterized by anti-nuclear IgG autoantibody (ANA-IgG) prod...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142238
更新日期:2012-07-01 00:00:00
abstract::In this study, we investigated whether the phenotype-related differentiation of human 2H4+ (CD45R) naive cells to 2H4- (CDw29) memory CD4 cells corresponded to modulation of interleukin (IL) 2, IL 4, interferon (IFN)-gamma and granulocyte-monocyte colony-stimulating factor (GM-CSF) gene expression, a phenomenon which ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190908
更新日期:1989-09-01 00:00:00
abstract::The present study tests whether the specific inhibition of helper T (Th) cell (and T hybridomas) by suppressor T (Ts) cells is a phenotypic trait of Th cells correlating with their acquired specificity for antigen/major histocompatibility complex or a genotypic trait not related to selection of the T cell repertoire f...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180415
更新日期:1988-04-01 00:00:00
abstract::Interleukin (IL)-6 transsignaling plays a pivotal role in the shift from neutrophil to monocyte recruitment at the inflammatory site. Release of neutrophil IL-6 receptor-alpha (IL-6Ralpha, CD126) in its soluble form is a key step of IL-6 transsignaling, however, its physiological inducers are poorly known. Here, we ob...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2002010)32:10<2965::AID-IMMU2965
更新日期:2002-10-01 00:00:00
abstract::We generated cell hybrids between mouse T cell lymphoma EL4 cells and mouse fibroblast B82 cells (BELIII and BELIV) to examine the expression of T cell receptor (TcR)/CD3 genes and their lymphoid-specific transcription factor genes, which are normally detected in EL4 cells. In BELIII and BELIV, expression of the TcR a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250947
更新日期:1995-09-01 00:00:00
abstract::The purpose of this study was to obtain additional information on the mechanism by which interferon-gamma (IFN-gamma) is able to regulate gene expression in macrophages. The expression of the genes for class II histocompatibility I-A beta, tumor necrosis factor (TNF) and complement component C3 was assayed after treat...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190621
更新日期:1989-06-01 00:00:00
abstract::The cytotoxic response of cell sorter-purified small Lyt-2+ splenic cytotoxic lymphocyte precursors from 10 individual C57BL/6 mice to mutant class I H-2Kbm1 or H-2Dbm14 allodeterminants was analyzed under limiting dilution conditions. The cytotoxic activity of anti-bm1-specific or anti-bm14-specific cytotoxic T lymph...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830161220
更新日期:1986-12-01 00:00:00
abstract::The CD45RO phenotype of peripheral blood CD4+ and CD8+ lymphocytes were determined in physically active males (n = 171) and females (n = 70) ranging in age from 15-68 years. Direct immunofluorescence and dual color flow cytometry was performed for analysis. Absolute cell numbers (r = 0.473) and percentages of CD4+ CD4...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231049
更新日期:1993-10-01 00:00:00
abstract::Paxillin is an adaptor protein associated with focal adhesion complex, and is activated by tyrosine phosphorylation through focal adhesion kinase (FAK) and Src kinase. Recent studies reveal that serine phosphorylation of paxillin by JNK and p38 MAPK is essential for cell migration or neurite extension, but their cellu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142192
更新日期:2012-08-01 00:00:00
abstract::A majority of immunodominant epitopes of mycobacterial antigens are known to be recognized by murine T cells in the context of several H-2 haplotypes. In this study, we established the frequency of T cells able to recognize these peptides promiscuously, i.e. in the context of allogeneic antigen-presenting cells, using...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251009
更新日期:1995-10-01 00:00:00
abstract::The thymus is a primary lymphoid organ supporting the development of self-tolerant T cells. Key events in T-cell development in the thymus include lineage commitment, selection events, and thymic emigration. This review discusses the proposed role of sphingosine-1-phosphate and its receptors in the emigration of both ...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.200838912
更新日期:2009-04-01 00:00:00
abstract::In this study the genetic control of major histocompatibility complex (MHC) class II gene expression during the transition from B cell to plasma cell has been analyzed. Class II molecules are not expressed in plasma cells because of an active suppression resulting in the abrogation of class II gene transcription. We s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261028
更新日期:1996-10-01 00:00:00
abstract::Interleukin-15 (IL-15) is an inflammatory cytokine whose role in autoimmune diseases has not been fully elucidated. Th17 cells have been shown to play critical roles in experimental autoimmune encephalomyelitis (EAE) models. In this study, we demonstrate that blockade of IL-15 signaling by TMβ-1 mAb treatment aggravat...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201444675
更新日期:2014-11-01 00:00:00
abstract::Maintenance of peripheral tolerance and inactivation of autoreactive T cells is based on a delicate balance between pro-inflammatory and protective cytokines that is poorly understood. We have here addressed how the local expression of the inflammatory cytokine TNF-alpha can impair peripheral tolerance and lead to aut...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323328
更新日期:2003-05-01 00:00:00
abstract::Studies of experimental autoimmune encephalomyelitis (EAE) in rodents have revealed that encephalitogenic T cell lines reactive with myelin basic protein (BP) are frequently dominated by clones expressing a restricted T cell receptor repertoire. Using the rat EAE model, we have begun to examine the basis for clonal do...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250114
更新日期:1995-01-01 00:00:00
abstract::DNA vaccination is an efficient way to induce CD8+ T cell memory, but it is still unclear to what extent such memory responses afford protection in vivo. To study this, we induced CD8+ memory responses directed towards defined viral epitopes, using DNA vaccines encoding immunodominant MHC class I-restricted epitopes o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323928
更新日期:2003-07-01 00:00:00
abstract::We have developed a quantitative assay for the measurement of class I assembly induced by peptide. We have applied this assay to H-2Db, Kb and HLA-A2.1 with a panel of 49 overlapping peptides derived from HIV-1 gag protein. We find that the effects of peptide on assembly form a continuous distribution. By defining pos...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210909
更新日期:1991-09-01 00:00:00
abstract::The problem of the structural relationship between suppressive IgG-binding factor and low-affinity receptors for the Fc portion of IgG (Fc gamma RII) has not yet been solved. In the present work we have isolated a recombinant soluble Fc gamma RII containing only the two external domains of Fc gamma RII, and analyzed i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191213
更新日期:1989-12-01 00:00:00
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journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201445400
更新日期:2015-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090306
更新日期:1979-03-01 00:00:00
abstract::A T cell activation antigen, Ly6C, is considered to be involved in the autoimmunity of some autoimmune-prone mice; however, the function of Ly6C remains largely unknown. We prepared a rat anti-mouse Ly6C monoclonal antibody (mAb) (S14) that inhibits the proliferation of peripheral T cells stimulated with anti-CD3 mAb ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199802)28:02<696::AID-IMMU
更新日期:1998-02-01 00:00:00
abstract::The immunocytochemical characteristics of large granular lymphocytes (LGL), isolated from the liver sinusoids of euthymic and athymic (nude) rats, were investigated in electron microscopy by the immunoperoxidase technique. The LGL were found positive for MRC OX-8 (natural killer cells and cytotoxic/suppressor T cells)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171006
更新日期:1987-10-01 00:00:00
abstract::In this study, we examined which cell population contributes to IL-5 production by Peyer's patch (PP) cells. Thy1.2(-) fraction of PP cells, but not those of splenocytes, secreted IL-5 in response to IL-2. We found that CD3epsilon(-)IL-2Ralpha(+) cells purified from the Thy1.2(-)B220(-) fraction of PP cells secreted I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324696
更新日期:2004-07-01 00:00:00
abstract::The host protective immune response to blood stage malaria infection was studied using Plasmodium chabaudi chabaudi (P.chabaudi) in NIH mice. It has been shown previously that CD4+ cells are critically required for protection against erythrocytic infection. Mice lacking a functional CD4+ cell compartment suffer unremi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240124
更新日期:1994-01-01 00:00:00
abstract::Monoclonal antibodies MRC-OX6 and MRC-OX3 were used to define biosynthetically inter-related subsets of I-A-equivalent class II molecules from Lewis rat spleen cells. MRC-OX6 was shown to recognize specifically multiple forms of the class II molecule arising along its maturation pathway from the origin of polypeptide ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830151216
更新日期:1985-12-01 00:00:00
abstract::Previous studies from our laboratory have shown that some in vitro maintained Leishmania major-specific L3T4+ T cells were capable of exacerbating cutaneous leishmaniasis after adoptive transfer to normal syngeneic mice. Results presented in this report show that these cells released substantial amounts of interleukin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180815
更新日期:1988-08-01 00:00:00
abstract::Listeria monocytogenes induces the formation of inflammasomes and subsequent caspase-1 activation, and the adaptor apoptosis-associated speck-like protein containing a CARD (ASC) is crucial for this response. However, the role of ASC in L. monocytogenes infection in vivo is unclear. In this study, we demonstrate that ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201444673
更新日期:2014-12-01 00:00:00
abstract::The development and the differentiation of CD8(+) T cells are dependent on IL-15. Here, we have studied the source and mechanism of how IL-15 modulates CD8(+) T cell-mediated Th1 immune responses by employing two delayed-type hypersensitivity (DTH) models. IL-15-deficient (IL-15(-/-)) mice or mice treated with soluble...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324545
更新日期:2003-12-01 00:00:00
abstract::DC not only activate CD4 T (Th) cell and cytotoxic CD8 T cell (CTL) responses against pathogens, but they also tolerize autoreactive T cells in order to avoid autoimmunity. Previous studies have demonstrated that steady-state DC can tolerize naïve CTL, naïve Th cells and memory CTL. A study in this issue of the Europe...
journal_title:European journal of immunology
pub_type:
doi:10.1002/eji.201040704
更新日期:2010-07-01 00:00:00
abstract::Interleukin-5 (IL-5) plays a critical role in the pathogenesis of eosinophil-associated allergic disorders, such as asthma. IL-5 may also play a major role in the development of eosinophilia-associated lymphoproliferative disorders caused by human T lymphotropic virus type I (HTLV-I). In this study, we have investigat...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200112)31:12<3694::aid-immu3694>
更新日期:2001-12-01 00:00:00