Abstract:
:Interleukin (IL)-7 and IL-2 are important lymphoproliferative cytokines which both use the gamma c chain as part of their respective receptors. To learn more of their signaling mechanisms a comparison was made of the patterns of intracellular tyrosine phosphorylated proteins induced by these cytokines in the murine T cell line, CT6. Several similarities were revealed in the tyrosine phosphorylated proteins induced. However, a notable subset of proteins of mainly < 60 kDa were only phosphorylated by IL-2. Characterization of the two most prominent bands of this subset, pp54 and pp42, revealed these to contain Shc and p42MAP/Erk kinase, respectively. Further studies confirmed that IL-7 was unable to induce the phosphorylation of either the p44MAP/Erk or p42MAP/Erk or activation of the kinases. Shc is involved in activation of p21ras, a key event in the signaling cascade, via p72raf and MEK, leading to MAP/Erk kinase (MAPK) activation. These data indicate that this pathway is not utilized by IL-7 and may not, therefore, be essential for cytokine-driven T cell proliferation. This possibility was supported by studies with the MEK inhibitor PD098059, which had no selective effect on CT6 proliferation induced by IL-2 as compared with IL-7, although the drug completely inhibited MAP/Erk phosphorylation induced by IL-2.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Crawley JB,Willcocks J,Foxwell BMdoi
10.1002/eji.1830261125subject
Has Abstractpub_date
1996-11-01 00:00:00pages
2717-23issue
11eissn
0014-2980issn
1521-4141journal_volume
26pub_type
杂志文章abstract::The regulation of nuclear factor (NF)-kappa B activation by the T cell receptor (TcR)/CD3 complex in primary human T cells has been studied at various times after activation. Only p50 NF-kappa B protein bound the kappa B element of interleukin-2 receptor (IL-2R) alpha chain promoter on resting T cells. However, immedi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250130
更新日期:1995-01-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181111
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journal_title:European journal of immunology
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更新日期:1993-08-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2001-03-01 00:00:00
abstract::Using sheep erythrocytes (SRBC) as the antigen, two subpopulations of spleen antigen-binding lymphocytes could be distinguished by a marked difference in the susceptibility of their receptors to trypsin. In unimmunized animals, 30% of the antigen-binding cells were trypsin-resistant, whereas at 5 days after immunizati...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050612
更新日期:1976-06-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737519
更新日期:2008-05-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199805)28:05<1727::AID-IMM
更新日期:1998-05-01 00:00:00
abstract::In this study, we demonstrated that antibodies reactive with a common beta 1 subunit (CD29) of the VLA family of antigens could synergize with CD3 but not CD2 in inducing CD4 cell proliferation in a serum-free culture system. The extent of activation was similar to that obtained when CD4 cells were cultured with anti-...
journal_title:European journal of immunology
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doi:10.1002/eji.1830210212
更新日期:1991-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201041266
更新日期:2011-05-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199902)29:02<548::AID-IMMU
更新日期:1999-02-01 00:00:00
abstract::The human type I interferon (IFN) receptor was characterized by ligand blotting. In this method, plasmalemma proteins or detergent-lysed whole-cell extracts from human Burkitt lymphoma cell lines were separated on polyacrylamide gels and subsequently transferred onto nitrocellulose sheets. Probing the blots with 3 x 1...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181221
更新日期:1988-12-01 00:00:00
abstract::Stilbene disulfonates are known to competitively inhibit Cl-/HCO3- flux through Band 3-related anion exchange (AE) proteins. To study the role of AE in lymphocyte activation, stilbene disulfonates were added to cultures of rat splenocytes (SPL). Four different stilbene derivatives were tested and each directly stimula...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220509
更新日期:1992-05-01 00:00:00
abstract::The viral CC chemokine macrophage inhibitory protein-II (vMIP-II) encoded by human herpes virus 8 (HHV-8) binds to multiple chemokine receptors, however, its ability to control the initial recruitment of specific leukocyte subtypes from the peripheral circulation has not been fully clarified. Here we show that vMIP-II...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200108)31:8<2458::aid-immu24
更新日期:2001-08-01 00:00:00
abstract::CD4+ helper T lymphocytes and CD8+ killer T lymphocytes are both generated in the thymus from common precursor cells expressing CD4 and CD8. The development of immature CD4 CD8+ thymocytes into mature 'single-positive' T cells requires T cell antigen-receptor (TCR)-mediated positive selection signals. Although it is k...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271231
更新日期:1997-12-01 00:00:00
abstract::Hypoxia and glucose deprivation are often observed in the microenvironment surrounding solid tumors in vivo. However, how they interfere with MHC class I antigen processing and CD8(+) T-cell responses remains unclear. In this study, we analyzed the production of antigenic peptides presented by classical MHC class I in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545835
更新日期:2016-04-01 00:00:00
abstract::Here we demonstrate that components of the entire complement cascade are fixed on the surface of erythrocytes infected with the human malarial parasite Plasmodium falciparum. Despite the activation of lytic complement factors, no complement-mediated lysis of P. falciparum-infected erythrocytes occurred only in the abs...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271034
更新日期:1997-10-01 00:00:00
abstract::It is widely believed that DC, but not macrophages, prime naïve T cells in vivo. Here, we investigated the ability of CD68-expressing cells (commonly defined as macrophages) in priming autoreactive T cells and initiating collagen-induced arthritis (CIA) in the mouse. For this purpose, a transgenic mouse was developed ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040598
更新日期:2011-02-01 00:00:00
abstract::Mice lacking the tumor suppressor gene p53 spontaneously develop T-cell lymphomas at a high rate, suggesting that in these mice lymphomas arise due to defective apoptosis mechanisms in T cells mediated by p53. However, a role of p53 in regulation of T-cell responses or apoptosis has been poorly defined. TCR-mediated s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939736
更新日期:2010-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1997-09-01 00:00:00
abstract::To understand further the roles that negative regulatory signals may play in B cell immune responses, we compared three inhibitors of B cell proliferation: cross-linking CD19 with monoclonal antibody (mAb), signaling through Fc receptors by intact anti-mu mAb, and transforming growth factor-beta (TGF-beta). Each agent...
journal_title:European journal of immunology
pub_type: 杂志文章
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200109)31:9<2811::aid-immu2811>3
更新日期:2001-09-01 00:00:00
abstract::The inositol phospholipid metabolism and the increase in cytosolic free Ca2+ concentration ([Ca2+]i) into the cell are recognized as two important events in the anti-mu-induced B cell activation. The anti-mu stimulation caused the [3H]inositol incorporation and also a rapid increase in [Ca2+]i from 85 nM to 285 nM. Th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170916
更新日期:1987-09-01 00:00:00
abstract::Activation of murine spleen cells in vitro with soluble anti-CD3 monoclonal antibody and phorbol 12-myristate 13-acetate (PMA) induced an initial production of interleukin 2 (IL2), interferon-gamma (IFN-gamma), IL4 and IL5, followed by a refractory state during which the T cells did not produce lymphokines when stimul...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210816
更新日期:1991-08-01 00:00:00
abstract::Transgenic CBA (H-2k haplotype) mice expressing the H-2 Kb major histocompatibility complex (MHC) class I gene under control of transcriptional promoter elements from a milk protein gene display high-level H-2 Kb transcription in lactating mammary glands and low-level transcription in skin and thymus of male and virgi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221027
更新日期:1992-10-01 00:00:00
abstract::It is now recognized that Th17 cells constitute the third effector arm of Th cells, complementing the Th1 and Th2 lineages. Although secretion of IL-17 is a primary defining characteristic of Th17 cells, the expression profile of specific chemokine receptors, cell surface molecules and cytokines are also part of the T...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
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更新日期:2009-06-01 00:00:00
abstract::We report here the identification and characterization of murine CCX-CKR, a high affinity receptor for the murine beta-chemokines SLC/mCCL21, MIP-3beta/mCCL19 and TECK/mCCL25. Unlike most other chemokine receptors, CCX-CKR is unable to mediate Ca(2+) fluxes upon ligand binding when expressed in HEK293 cells. Murine CC...
journal_title:European journal of immunology
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更新日期:2002-05-01 00:00:00
abstract::In this study the genetic control of major histocompatibility complex (MHC) class II gene expression during the transition from B cell to plasma cell has been analyzed. Class II molecules are not expressed in plasma cells because of an active suppression resulting in the abrogation of class II gene transcription. We s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261028
更新日期:1996-10-01 00:00:00
abstract::Lymphotoxin (LT)alpha in combination with LTbeta forms membrane-bound heterotrimeric complexes with a crucial function in lymph node (LN) organogenesis and correct morphogenesis of secondary lymphoid tissue. To study the role of membrane LT (mLT) in lymphoid tissue organogenesis we generated an LTbeta-deficient mouse ...
journal_title:European journal of immunology
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doi:10.1002/1521-4141(200207)32:7<1993::AID-IMMU1993>3
更新日期:2002-07-01 00:00:00
abstract::Previous work has shown that the primary IgM plaque-forming cell response of inbred mice to xenogeneic red blood cells (RBC) including sheep, horse and chicken RBC is under the control of two polymorphic genes or sets of genes, one linked to the Igh linkage group and the other of unknown linkage but unlinked to H-2 an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150205
更新日期:1985-02-01 00:00:00
abstract::The expression of interferon (IFN) β is highly induced in immune and nonimmune cells in response to virus infection. This upregulation is mediated at both transcriptional and posttranscriptional levels. Whereas the signaling pathways triggered by innate virus receptors leading to transcription factor activation have b...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201545616
更新日期:2015-05-01 00:00:00