Abstract:
:At the surface of the murine T lymphoma cell line RMA-S, the expression of "empty" class I molecules can be dramatically enhanced by culture at 26 degrees C. These class I molecules are unstable following transfer to 37 degrees C unless they are loaded with exogenously added peptides. Class I heterodimers that have failed to bind peptide ("empty" class I molecules) dissociate and the class I heavy chains are degraded. Internalization, if it precedes breakdown, would be the rate-limiting step. Radioiodinated peptides (VSV NP 8-mer or Sendai NP 9-mer) dissociate from the class I molecules in the absence of exogenous peptide or beta 2 microglobulin and appear in the medium. Release of the iodinated peptides does not result in a reduction in the quantity of stable assembled class I molecules. This paradox may be explained by a more rapid off-rate for radioiodinated peptides, when compared with their unlabeled counterparts, which constitute about 99% of the total in our radiolabeled preparations. In the medium the peptide is rapidly modified by serum- and cell-derived proteases. The short half-life of empty class I molecules and of free ligand would effectively preclude sensitization of innocent bystanders for lysis by cytotoxic T lymphocytes.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Neefjes JJ,Smit L,Gehrmann M,Ploegh HLdoi
10.1002/eji.1830220639keywords:
subject
Has Abstractpub_date
1992-06-01 00:00:00pages
1609-14issue
6eissn
0014-2980issn
1521-4141journal_volume
22pub_type
杂志文章abstract::The T cell mitogens, concanavalin A and the monoclonal antibody OKT3, cause a rapid activation of ornithine decarboxylase (ODC) activity in human T lymphocytes, maximal within 10 min of mitogen addition. Nonmitogenic ligands to T cell surface structures do not induce ODC. The enzyme induction is dependent upon an inta...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150808
更新日期:1985-08-01 00:00:00
abstract::A cDNA coding for the human interleukin 1 receptor antagonist protein (IL 1Ra) was used to clone the corresponding murine cDNA. The nucleotide sequence of the open reading frame coding for the processed form of mIL 1Ra predicted a 152-residue protein that was 77% identical to human IL 1Ra. The cellular and tissue dist...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830211119
更新日期:1991-11-01 00:00:00
abstract::Normal human peripheral blood lymphocytes were shown to activate complement in normal human serum (NHS). This activation led to C3 fixation on the cell membrane, which in turn was visualized by fluorescence. The reaction occurred in the absence of detectable amounts of antibodies, since an agammaglobulinemic patient's...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090115
更新日期:1979-01-01 00:00:00
abstract::The immunoglobulin lambda light chain system displays a limited diversity in inbred mice. Indeed, the lambda locus is organized in two recombination units: V lambda 2-V lambda x-J lambda 2-C lambda 2-psi J lambda 4-psi C lambda 4, which can produce either lambda 2(V2) or lambda 2(Vx) chains; and V lambda 1-J lambda 3-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240912
更新日期:1994-09-01 00:00:00
abstract::At least two subsets of CD4+ T helper cell lymphocytes termed Th1 and Th2 exist in the mouse and probably in humans. They are characterized by the secretion of different lymphokines and by their functional behavior. Dysregulated expansion of one or the other subset may be one reason for the development of certain dise...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230805
更新日期:1993-08-01 00:00:00
abstract::Mouse thymus-leukemia antigen (TL), like other major histocompatibility complex (MHC) class I-b antigens, displays signs of a specialized function. It is normally expressed at high levels on immature thymocytes and at moderate levels on gut epithelium and activated mature T cells. A promoter/enhancer region unique amo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250421
更新日期:1995-04-01 00:00:00
abstract::Atopic dermatitis (AD) or eczema is the most common chronic inflammatory skin disease. It is a multifactorial disease with local and systemic immune changes. Current therapies focus on restoring the local skin barrier or inhibiting immune responses. In this issue of the European Journal of Immunology, Sehra et al. [Eu...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
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abstract::Human papillomavirus (HPV) infections account for more than 50% of infection-linked cancers in women worldwide. The immune system controls, at least partially, viral infection and around 90% of HPV-infected women clear the virus within two years. However, it remains unclear which immune cells are implicated in this pr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141693
更新日期:2011-11-01 00:00:00
abstract::gp140 is the Epstein-Barr virus receptor and the C3d receptor (EBVR/C3dR) of human B lymphocytes. Recently, we have shown that cross-linking of EBVR/C3dR on cell surface by polyclonal anti-gp140 induced B cell activation, in presence of T cell factors. Immunoregulatory abnormalities of EBV-induced B cell activation ha...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830161108
更新日期:1986-11-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830081103
更新日期:1978-11-01 00:00:00
abstract::Glycosylphosphatidylinositol-anchored (GPI)-Db molecules are defective in mediating cytotoxic T lymphocytes (CTL) lysis of transfected lymphoma cells, compared to their transmembrane (TM) counterpart. This defect is manifest when antigenic peptide must be processed and presented through the endogenous pathway. These s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260938
更新日期:1996-09-01 00:00:00
abstract::Myeloid dendritic cells (DC) and macrophages evolve from a common precursor. However, factors controlling monocyte differentiation toward DC or macrophages are poorly defined. We report that the surface density of the GM-CSF receptor (GM-CSFR) alpha subunit in human peripheral blood monocytes varies among donors. Alth...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737395
更新日期:2008-03-01 00:00:00
abstract::The immune response against human adenovirus (HAdV) has gained interest because of the application of HAdV-based vectors in gene therapy and the high incidence of infections in pediatric recipients of allogeneic stem cell grafts. Because antiviral medication is frequently ineffective, the option of adoptive transfer o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535786
更新日期:2006-09-01 00:00:00
abstract::The recent discovery of IL-17-producing CD4(+) Th subset significantly revised the Th1/Th2 dichotomy model proposed by Mosmann and Coffman almost two decades ago. Th17 cells are involved in the pathogenesis of many human autoimmune diseases. Th17 cells, their developmental pathways and their effector functions, theref...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200839105
更新日期:2009-03-01 00:00:00
abstract::Mononuclear phagocytes and lymphocytes can both synthesize cytotoxins which kill certain tumor cell lines and recent evidence suggests that these cytotoxins may be identical. In this study, human monocyte cytotoxin and lymphoblastoid lymphotoxin have been critically compared in terms of specificity, physicochemical ch...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150321
更新日期:1985-03-01 00:00:00
abstract::The killer cell lectin-like receptor G1 (KLRG1) is a cadherin-binding inhibitory receptor expressed by NK cells and differentiated T cells. Here, we surprisingly found that a fraction of KLRG1 molecules expressed in the murine A5 T-cell line and in IL-2-activated NK cells forms disulfide-linked heteromers with the tra...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201344234
更新日期:2014-06-01 00:00:00
abstract::One of the clear paradoxes in tumor immunology is the fact that cross-presentation of cell-associated tumor antigens to CD8(+) T cells is efficient, yet CTL generation is weak, and tumors continue to grow. We examined, for the first time whether this may be due to alterations in the phenotype or function of cross-pres...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200940153
更新日期:2010-06-01 00:00:00
abstract::The death of individual cells is a frequent and physiological event in the mammalian immune system and most often occurs by apoptosis. It is becoming increasingly clear that cell death is also induced during bacterial infections. Here we report that, in addition to the apoptotic form already established, a necrosis-li...
journal_title:European journal of immunology
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doi:10.1002/eji.200324582
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abstract::Intravenous immunoglobulin G (IVIg) therapy is widely used to treat autoimmune and inflammatory diseases. Recent evidence suggests that in mice, splenic resident cells might be important for the anti-inflammatory activity of IVIg in a model of serum transfer arthritis. Splenectomized human immunothrombocytopenia (ITP)...
journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/immu.200310017
更新日期:2003-02-01 00:00:00
abstract::The capacity of bone marrow-derived surface immunoglobulin-positive (sIg+) human and mouse immature B cells, generated either in vitro or in vivo, to change their light (L) chain expression, has been assayed by the number of cells which change in vitro from one type of L chain to the other type, or to no sIg at all. I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251118
更新日期:1995-11-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830071104
更新日期:1977-11-01 00:00:00
abstract::Ligand binding to beta1-integrins exerts multiple effects on cells of the immune system including adhesion, spreading, haptotaxis and costimulation of T cells activated by anti-CD3. Here we show that a high-affinity ligand for beta1-integrins, the invasin (Inv) protein of Yersinia pseudotuberculosis, can induce cell d...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200204)32:4<1129::AID-IMMU1129>3
更新日期:2002-04-01 00:00:00
abstract::Activation of invariant natural killer T (iNKT) cells by treatment with their α-galactosyl ceramide ligand provides therapeutic benefits in several immune inflammatory settings. Given the artificial nature of this stimulation, the natural regulatory functions of iNKT remain uncertain. Addressing this issue in a mouse ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040647
更新日期:2011-02-01 00:00:00
abstract::LeIF, a gene homologue of the eukaryotic initiation factor 4A was first described as a leishmanial antigen that induced a Th1-type T cell response in peripheral blood mononuclear cells (PBMC) from leishmaniasis patients. Moreover, the interferon (IFN)-gamma production by PBMC was found to be interleukin (IL)-12 depend...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271024
更新日期:1997-10-01 00:00:00
abstract::Heterologous adenovirus-based vectors hold promise as preventative HIV vaccines but their capacity to induce effective T-cell immunity in established infection has not been explored. We vaccinated rhesus macaques chronically infected with SIVmac251 and undergoing antiretroviral therapy (ART) with human adenovirus sero...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200839130
更新日期:2009-09-01 00:00:00
abstract::The interaction of KB-V1, a multidrug resistant (MDR) variant of the KB-3-1 human oral carcinoma, with human complement was investigated. KB-V1 cells were found to be more sensitive than KB-3-1 cells to complement-mediated lysis. Detailed analysis of the capacity of KB cells to activate human complement demonstrated t...
journal_title:European journal of immunology
pub_type: 杂志文章
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abstract::The analysis of an Ia specificity was performed in several H-2 haplotypes by evaluating the affinity of the binding to mouse spleen cells of a monoclonal anti-Ia alloantibody. We used the monoclonal antibody 10-2.16 (Oi et al., Curr. Top. Microbiol. Immunol. 1978. 81: 115.) which recognizes on I-A gene products a publ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110912
更新日期:1981-09-01 00:00:00
abstract::Major advances in preventing, delaying, or curing individual pathologies are responsible for an increasingly long life span in the developed parts of our planet, and indeed reaching eight to nine decades of life is nowadays extremely frequent. However, medical and sanitary advances have not prevented or delayed the un...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
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更新日期:2016-10-01 00:00:00
abstract::The presentation of islet antigens in the pancreatic LNs (PLNs) of mice is a developmentally regulated process. It has been hypothesized that, during physiological tissue remodeling, a wave of neonatal β-cell apoptosis may initiate diabetes in autoimmune-prone strains of mice. If true, increasing or decreasing physiol...
journal_title:European journal of immunology
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doi:10.1002/eji.201141476
更新日期:2011-08-01 00:00:00