Abstract:
:The capacity of bone marrow-derived surface immunoglobulin-positive (sIg+) human and mouse immature B cells, generated either in vitro or in vivo, to change their light (L) chain expression, has been assayed by the number of cells which change in vitro from one type of L chain to the other type, or to no sIg at all. Immature sIg+ B cells were generated in vitro from sIg- precursor cells from human or mouse bone marrow. The immature sIg+ cells expressed RAG-1. Human sIg+ cells expressed kappa and lambda L chains in ratios between 1:1 and 3:1, whereas in mouse cells, this ratio ranged from 10:1 to 20:1. Upon reculture of the human and mouse kappa+ sIg+ cells, about half of them remained kappa+, a quarter became lambda+, and another quarter became sIg-. Between 1 and 3% expressed both kappa and lambda chains. Of the human lambda+ cells, about two-thirds remained lambda+, only 1 to 2% became kappa+, while the other third became sIg-. Again, between 1 and 3% expressed both kappa and lambda L chains. These results indicate that expression of sIgM in the B cell membrane does not terminate L chain gene rearrangement, and that some order exists in kappa versus lambda gene rearrangements. Hence, human and mouse kappa+ immature B cells can become lambda+, but very few of the lambda+ cells can become kappa+, and both can become sIg-. Further, human CD10+/sIg+ kappa+ and lambda+ cells and mouse B220low/sIglow kappa+ cells enriched from bone marrow, i.e. immature B cells differentiated in vivo, changed their Ig phenotype upon in vitro culture, but in lower frequencies. By contrast, human and mouse mature B cells did not change their L chain or Ig phenotype. Hence, at least a part of the sIg+ immature B cells in bone marrow retain the capacity to change their L chain and Ig phenotype, and this capacity is lost when they become mature, peripheral B cells.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Ghia P,Gratwohl A,Signer E,Winkler TH,Melchers F,Rolink AGdoi
10.1002/eji.1830251118subject
Has Abstractpub_date
1995-11-01 00:00:00pages
3108-14issue
11eissn
0014-2980issn
1521-4141journal_volume
25pub_type
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