Peritoneal exudate lymphocyte and mixed lymphocyte culture hybridomas are cytolytic in the absence of cytotoxic cell proteinases and perforin.

abstract：:An association between T-cell lymphopenia and autoimmunity has long been proposed, but it remains to be elucidated whether T-cell lymphopenia affects B-cell responses to autoantigens. Human neonatal thymectomy (Tx) results in a decrease in T-cell numbers and we used this model to study the development of autoreactivit...

journal_title：European journal of immunology

authors： van den Broek T,Madi A,Delemarre EM,Schadenberg AWL,Tesselaar K,Borghans JAM,Nierkens S,Redegeld FA,Otten HG,Rossetti M,Albani S,Sorek R,Cohen IR,Jansen NJG,van Wijk F

更新日期：2017-11-01 00:00:00

abstract：:T90/44 is a cell surface antigen which is present on human T cells of the helper and cytotoxic subsets and which binds the 9.3 monoclonal antibody (9.3 mAb). It is expressed in the form of 90-kDa disulfide-bonded dimers of a 44-kDa polypeptide and of free 44-kDa subunits. The function of T90/44 was investigated in a s...

journal_title：European journal of immunology

authors： Lesslauer W,Koning F,Ottenhoff T,Giphart M,Goulmy E,van Rood JJ

更新日期：1986-10-01 00:00:00

abstract：:The modulatory activity of dextran sulfate with a relative molecular mass of 8 and 500 kDa and pentosan polysulfate with a relative molecular mass of 6 kDa on human T cell surface molecules CD2, CD3, CD4, CD8 and HLA-DR was investigated by analytical flow cytometry. The 500-kDa dextran sulfate induces a complete disap...

journal_title：European journal of immunology

authors： Thiele B,Braig HR,Ehm I,Kunze R,Ruf B

更新日期：1989-06-01 00:00:00

abstract：:Proliferating cells require iron and, therefore, express the transferrin receptor (CD71) that mediates cellular iron uptake. Cycling thymocytes, which have the CD4-8-3-, CD4-8+3-, or CD4+8+3- phenotypes, also express CD71. The importance of CD71-mediated iron uptake for proliferation and maturation of thymocytes was s...

journal_title：European journal of immunology

authors： Brekelmans P,van Soest P,Leenen PJ,van Ewijk W

更新日期：1994-11-01 00:00:00

abstract：:The specificity and efficiency of endogenous peptides in the HLA class I binding have been investigated by the use of a simple procedure that is based on the serological detection of the folding of HLA class I alpha chains that is induced by the binding to specific peptides in the presence of beta 2 microglobulin. HLA...

journal_title：European journal of immunology

authors： Tanigaki N

更新日期：1992-08-01 00:00:00

abstract：:Several drugs targeting members of the TNF superfamily or TNF receptor superfamily (TNFRSF) are widely used in medicine or are currently being tested in therapeutic trials. However, their mechanism of action remains poorly understood. Here, we explored the effects of TNFRSF co-stimulation on murine Foxp3+ regulatory T...

journal_title：European journal of immunology

authors： Lubrano di Ricco M,Ronin E,Collares D,Divoux J,Grégoire S,Wajant H,Gomes T,Grinberg-Bleyer Y,Baud V,Marodon G,Salomon BL

更新日期：2020-07-01 00:00:00

abstract：:This study reports on the earliest stages of mononuclear phagocyte differentiation. A crucial question in this developmental process is whether mature macrophage (M phi) heterogeneity is already appointed at the precursor cell level. For this purpose, we produced clonal populations of mononuclear phagocytes from bone ...

journal_title：European journal of immunology

authors： Leenen PJ,Slieker WA,Melis M,Van Ewijk W

更新日期：1990-01-01 00:00:00

abstract：:After incorporation of 14C-labeled amino acids and/or of [3H]fucose during in vitro culture of the Thy-1.1-bearing, Fc receptor-positive T lymphoma, L-5187-Y, attempts were made to purify the Fc binding structure(s). Following solubilization of a crude membrane pellet using sodium deoxycholate, the 110 000 x g superna...

journal_title：European journal of immunology

authors： Frade R,Kourilsky FM

更新日期：1977-09-01 00:00:00

abstract：:The immunosuppressor sanglifehrin A (SfA) is a member of a family of immunophilin cyclophilin A-binding molecules and does not inhibit calcineurin activity. Sanglifehrin A inhibits M-CSF-dependent macrophage proliferation by arresting the G1 phase of the cell cycle but does not affect cell viability. This immunosuppre...

journal_title：European journal of immunology

authors： Sànchez-Tilló E,Wojciechowska M,Comalada M,Farrera C,Lloberas J,Celada A

更新日期：2006-09-01 00:00:00

abstract：:The MHC class II molecule H2-A(s), expressed in the SJL mouse strain, is the principle restriction element of autoreactive CD4(+) T cells mediating experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis. We deduced the H2-A(s) ligand motif from the analysis of naturally processed self peptide...

journal_title：European journal of immunology

authors： Kalbus M,Fleckenstein BT,Offenhäusser M,Blüggel M,Melms A,Meyer HE,Rammensee HG,Martin R,Jung G,Sommer N

更新日期：2001-02-01 00:00:00

abstract：:The structure of immunological synapses formed between murine naive T cells and mature dendritic cells has been subjected to a quantitative analysis. Immunofluorescence images of synapses formed in the absence of antigen show a diffuse synaptic accumulation of CD3 and LFA-1. In electron microscopy, these antigen-free ...

journal_title：European journal of immunology

authors： Brossard C,Feuillet V,Schmitt A,Randriamampita C,Romao M,Raposo G,Trautmann A

更新日期：2005-06-01 00:00:00

abstract：:A major contributing factor to the final magnitude and breadth of CD8(+) T-cell responses to complex antigens is immunodomination, where CD8(+) T cells recognizing their cognate ligand inhibit the proliferation of other CD8(+) T cells engaged with the same APC. In this study, we examined how the half-life of cell s...

journal_title：European journal of immunology

authors： Galea I,Stasakova J,Dunscombe MS,Ottensmeier CH,Elliott T,Thirdborough SM

更新日期：2012-01-01 00:00:00

abstract：:Cholera toxin (CT) can function as a potent adjuvant in the mucosal immune response. However, we have found that treatment of A20-HL murine B lymphoma cells with CT severely inhibits the presentation of ovalbumin (OVA) to cells of the T cell clone 42-6A specific for OVA(323-339)/I-Ad, whereas it does not affect the pr...

journal_title：European journal of immunology

authors： Tanaka Y,Nakano H,Ishikawa F,Yoshida M,Gyotoku Y,Kakiuchi T

更新日期：1999-05-01 00:00:00

abstract：:BALB/c mice were repeatedly immunized with microgram doses of benzylpenicilloylated Ascaris protein(s) (BPO9Asc) in alum. At different stages of the immune response, BPO21 eicosa-L-lysine or two analogs containing one or two hydrophobic p-oxymethylbenzyl-3 beta-cholestanyl succinate (OSuco) groups were injected. When ...

journal_title：European journal of immunology

authors： Lüscher IF,Weber E,de Weck AL

更新日期：1984-01-01 00:00:00

abstract：:The activity of granzyme B, a main effector molecule of cytotoxic T lymphocytes (CTL) and natural killer cells, is regulated by the human intracellular serpin proteinase inhibitor 9 (PI9). This inhibitor is particularly expressed by CTL and dendritic cells, in which it serves to protect these cells against endogenous ...

journal_title：European journal of immunology

authors： Bladergroen BA,Strik MC,Wolbink AM,Wouters D,Broekhuizen R,Kummer JA,Hack CE

更新日期：2005-04-01 00:00:00

abstract：:Activated human T cells via the CD2 or the CD3 pathways exhibited a higher capacity than resting T lymphocytes to incorporate and metabolize [3H]pafacether (paf) at 37 degrees C. Resting T lymphocytes lacked specific binding capacity for paf, yet high-affinity paf receptors (paf-R) were induced on CD3- or CD2-dependen...

journal_title：European journal of immunology

authors： Calabresse C,Nguer MC,Pellegrini O,Benveniste J,Richard Y,Thomas Y

更新日期：1992-06-01 00:00:00

abstract：:During the course of many human autoimmune diseases, antibodies which recognize negatively charged epitopes on self antigens are detected. Trypanosoma cruzi, an intracellular protozoan parasite capable of infecting a wide variety of vertebrates, is the cause of Chagas disease in humans. Infection with the parasite fre...

journal_title：European journal of immunology

authors： Kahn S,Kahn M,Eisen H

更新日期：1992-12-01 00:00:00

abstract：:Fever is associated with improved survival during infection in endothermic and ectothermic species although the protective mechanisms are largely undefined. Previous studies indicate that fever-range thermal stress increases the binding activity of the L-selectin homing receptor in human or mouse leukocytes, thereby p...

journal_title：European journal of immunology

authors： Appenheimer MM,Girard RA,Chen Q,Wang WC,Bankert KC,Hardison J,Bain MD,Ridgley F,Sarcione EJ,Buitrago S,Kothlow S,Kaspers B,Robert J,Rose-John S,Baumann H,Evans SS

更新日期：2007-10-01 00:00:00

abstract：:Two different subpopulations of anti-acetylcholine receptor antibodies were studied during the evolution of experimental autoimmune myasthenia in one rabbit immunized with Torpedo acetylcholine receptor. The results show that the subpopulation of antibodies directed against the toxin-binding site of the receptor might...

journal_title：European journal of immunology

authors： Zurn AD,Fulpius BW

更新日期：1977-08-01 00:00:00

abstract：:The development and the differentiation of CD8(+) T cells are dependent on IL-15. Here, we have studied the source and mechanism of how IL-15 modulates CD8(+) T cell-mediated Th1 immune responses by employing two delayed-type hypersensitivity (DTH) models. IL-15-deficient (IL-15(-/-)) mice or mice treated with soluble...

journal_title：European journal of immunology

authors： Rückert R,Brandt K,Bulanova E,Mirghomizadeh F,Paus R,Bulfone-Paus S

更新日期：2003-12-01 00:00:00

abstract：:The transferrin receptor (TfR) was recently proposed as putative natural killer (NK) cell target structure. Here data are presented against this hypothesis and it is shown that low TfR expression and high NK sensitivity can occur concommitantly . K562 cells were studied at various stages of cell proliferation. No chan...

journal_title：European journal of immunology

authors： Dokhélar MC,Garson D,Testa U,Tursz T

更新日期：1984-04-01 00:00:00

abstract：:The recently discovered B6.C-H-2bm12 (bm12) histocompatibility mutant bears a mutation of the I-A subregion of the H-2b haplotype. We have studied cytotoxic T lymphocytes (CTL) generated in secondary mixed lymphocyte culture (MLC) between the bm12 mutant and the strain of origin C57BL/6 (B6). In both directions, stron...

journal_title：European journal of immunology

authors： de Waal LP,Melief CJ,Melvold RW

更新日期：1981-03-01 00:00:00

abstract：:The 4-1BB is a costimulatory molecule similar to the receptor activator of NF-kappaB ligand (RANKL), both of which are key factors for the differentiation of osteoclasts and are expressed mainly by activated T cells. The 4-1BB shares common signaling pathways with RANK, suggesting a potential role in osteoclastogenesi...

journal_title：European journal of immunology

authors： Yang J,Park OJ,Lee YJ,Jung HM,Woo KM,Choi Y

更新日期：2008-06-01 00:00:00

abstract：:APO-1/Fas (CD95) is a transmembrane receptor that transduces apoptotic signals within various cells including T and B cells. The APO-1 gene was found to be defective in lpr mice. In these mice insertion of a retrotransposon gives rise to transcription of an abnormal mRNA and only a fraction of wild-type APO-1 mRNA. It...

journal_title：European journal of immunology

authors： Mariani SM,Matiba B,Armandola EA,Krammer PH

更新日期：1994-12-01 00:00:00

abstract：:As we approach the centenary of Elie Metchnikoff's Nobel Prize (1908), it is opportune to reflect upon the history of macrophage immunobiology, take stock of current knowledge and anticipate questions for the future. Starting from his appreciation of phagocytosis as an important determinant of host defence against inf...

journal_title：European journal of immunology

authors： Gordon S

更新日期：2007-11-01 00:00:00

abstract：:A 2-kDa synthetic derivative of the macrophage-activating lipopeptide (MALP-2) from Mycoplasma fermentans is a potent inducer of monocytes/macrophages and improves the immunogenicity of antigens co-administered by systemic and mucosal routes. Dendritic cells (DC) are the most potent antigen-presenting cells, which are...

journal_title：European journal of immunology

authors： Link C,Gavioli R,Ebensen T,Canella A,Reinhard E,Guzmán CA

更新日期：2004-03-01 00:00:00

abstract：:RNA from 170 different Ig-secreting hybridomas was hybridized with VH gene probes 7183, QUPC52, S107, J558, J606, 36-60, V31, X-24 and V-GAM 3.8, with the aim of comparing xid to non-xid mice, neonatal NZB to adult NZB, and the peritoneal cavity with the splenic compartments for VH gene family expression. Our results ...

journal_title：European journal of immunology

authors： Dighiero G,Lim A,Lembezat MP,Kaushik A,Andrade L,Freitas A

更新日期：1988-12-01 00:00:00

abstract：:The fine specificity of murine B 10.A/SgSn (B 10.A) T cells reactive with hen egg-white lysozyme (HEL) has been studied through the use of reduced, carboxymethylated HEL, a set of peptides encompassing the entire molecule, and a set of variant lysozymes from other species. Cells were taken from the lymph nodes drainin...

journal_title：European journal of immunology

authors： Maizels RM,Clarke JA,Harvey MA,Miller A,Sercarz EE

更新日期：1980-07-01 00:00:00

abstract：:Macrophage-chemotactic factor (MChF)-producing T cell hybridomas were established. These hybridomas produced MChF upon the specific antigenic stimulation with phosphorylcholine (PC)-conjugated protein in the presence of I-Ed-positive antigen-presenting cells. The hybridomas also secreted MChF after co-culture with con...

journal_title：European journal of immunology

authors： Harita S,Sugimura K,Watanabe T

更新日期：1984-08-01 00:00:00

abstract：:It has long been recognized that T cells in the cerebrospinal fluid (CSF) and other inflammatory compartments cannot be stimulated by mitogen and the reason for this has remained unknown. This question was investigated using mononuclear cells (MNC) isolated from the CSF of subjects with multiple sclerosis and other in...

journal_title：European journal of immunology

authors： Chofflon M,González V,Weiner HL,Hafler DA

更新日期：1989-10-01 00:00:00