Abstract:
:The inheritance of responsiveness to lipopolysaccharide (LPS), and of a marker recognized in LPS-reactive cells by a heterologous antiserum, was studied in crosses between C3H/HeJ (nonresponder) and C3H/Tif (high responder) mice. F1 hybrid mice show codominant expression of these traits: (a) LPS-reactive cells are only hlaf as frequent in the hybrids as in the high responder parent; (b) the serologically defined marker is expressed in half as many cells in the hybrids as in the high responder parent. In backcross generations, both LPS responsiveness and this serological marker segregated into high, intermediate, and nonresponders. LPS or free lipid A, but not two other B cell mitogens (lipoprotein, and purified protein derivative of tuberculin), compete with the antiserum for binding to the B cell surface membrane, and are capable of completely inhibiting such binding without interfering with the binding of a-ti-Ig antibodies or complexes to Fc receptors. The addition of an IgG fraction of the antiserum to B cell cultures results in exponetial growth of the cells and in maturation to antibody secretion. This mitogenic activity is dose-depedent and absorbable on spleen cells from LPS high responder mice. Taken together, these observations suggest that this antiserum contains antibodies to the lipid A-specific triggering receptor on B lymphocytes.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Coutinho A,Forni L,Watanabe Tdoi
10.1002/eji.1830080113subject
Has Abstractpub_date
1978-01-01 00:00:00pages
63-7issue
1eissn
0014-2980issn
1521-4141journal_volume
8pub_type
杂志文章abstract::Fc gamma receptor-bearing U937 cells, when incubated in serum-free buffer, were found to release spontaneously a suppressor material for pokeweed mitogen-drigen IgG synthesis which could be retained on Sepharose 4B-IgG immunosorbents. Immunosorbents coupled with IgM or F (ab')2 fragments of IgG were unable to retain t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160106
更新日期:1986-01-01 00:00:00
abstract::T cell-depleted B cells from a patient with LFA-1 deficiency were tested in costimulation assays for responsiveness to recombinant human IL4 (BSF-1) and purified low molecular weight B cell growth factor (BCGFlow). In both cases the response of LFA-1-deficient B cells was comparable with normal controls. Monoclonal an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180212
更新日期:1988-02-01 00:00:00
abstract::We investigated whether percutaneous sensitization with different allergens through barrier-disrupted skin regulates the balance of Th1/Th2 cytokine expression. When mice were sensitized with the typical hapten picryl chloride (PiCl) by a single topical application to intact skin, there was an up-regulation in the lym...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199803)28:03<769::AID-IMMU
更新日期:1998-03-01 00:00:00
abstract::The expression of human leukocyte function-associated antigens (HLFA, equivalents of murine LFA-1) was studied on early lympho-hemopoietic cells in infant thymus and normal bone marrow by double immunofluorescence methods, cell sorting and colony-forming assays. Monoclonal antibodies MHM-24 recognizing the alpha chain...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160513
更新日期:1986-05-01 00:00:00
abstract::Good responder (C57/Br/cd) and poor responder (C57BL/10) mice were immunized with mouse thyroid extract in Freund's complete adjuvant. The good responder mice first showed antibody to thyroglobulin on the seventh day while the poor responder animals had slightly lower titers and antibody did not appear until day 12. T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830070110
更新日期:1977-01-01 00:00:00
abstract::Retinoic acid (RA), which is the biologically active form of vitamin A, acts through the nuclear hormone receptor RAR (RA receptor) to induce either gene activation or repression. RA production and its effects have been linked to macrophages, dendritic cells, T and B cells, and iNKT cells in the immune system and play...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242705
更新日期:2012-07-01 00:00:00
abstract::Acute inflammatory activation of macrophages by Toll-like and related receptors is characterized by transient activation of MAPK-, NF-κB- and IRF-mediated signaling pathways and expression of pro-inflammatory genes. This activation state is inherently unstable and often transitions into a state of 'tolerance' characte...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201141783
更新日期:2011-09-01 00:00:00
abstract::To study allelic exclusion of TcR genes we analyzed two types (I and II) of TcR beta transgenic mice. T cells derived from both types of mice contained similar amounts of transgenic RNA transcripts; however, surface expression of the transgenic beta chain was drastically reduced in type II compared to type I. In type ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200227
更新日期:1990-02-01 00:00:00
abstract::Young (less than 3 months of age) and old (greater than 1 year of age) C.B-17 scid/scid mice were tested for the presence of immunoglobulin in serum and CD3+ T cells in spleen and peritoneal cavity. In all old severe combined immune deficiency (scid) mice tested we found detectable, but very variable levels of serum i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210240
更新日期:1991-02-01 00:00:00
abstract::Several drugs targeting members of the TNF superfamily or TNF receptor superfamily (TNFRSF) are widely used in medicine or are currently being tested in therapeutic trials. However, their mechanism of action remains poorly understood. Here, we explored the effects of TNFRSF co-stimulation on murine Foxp3+ regulatory T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201948393
更新日期:2020-07-01 00:00:00
abstract::The infiltration and activation of monocytes is a hallmark of chronic inflammation, including that associated with a variety of disease states such as rheumatoid arthritis, atherosclerosis, and various autoimmune conditions. Recently, a family of small molecular mass proteins has been described which appear to have in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220621
更新日期:1992-06-01 00:00:00
abstract::Leishmaniasis is currently classified as category 1 disease, i.e. emerging and uncontrolled. Since the importance of persistent infection for maintaining an effective long-lasting protective response is controversial, the present study asks whether immunisation with non-persistent parasites leads to protection against...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838423
更新日期:2008-11-01 00:00:00
abstract::Activation pathways inducing the expression of the interferon (IFN)-gamma gene in a cytotoxic T lymphocyte (CTL) clone were studied for their effects on transcription and on mRNA stability. IFN-gamma was secreted by the CTL clone in response to the Ca2+ ionophore ionomycin when used in conjunction with either protein ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250405
更新日期:1995-04-01 00:00:00
abstract::In the fetus the peripheral T cell pool expands as the fetus grows, but the mechanisms that regulate T cell homeostasis during fetal life are unknown. Here, we show that the peripheral T cell pool in the sheep fetus is established by the export from the fetal thymus of twice as many CD8+ as CD4+ thymic emigrants every...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636096
更新日期:2006-10-01 00:00:00
abstract::In the bone marrow (BM), memory plasma cells (PCs) survive for long time periods in dedicated microenvironmental survival niches, resting in terms of proliferation. Several cell types, such as eosinophils and reticular stromal cells, have been reported to contribute to the survival niche of memory PCs. However, until ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201344313
更新日期:2014-08-01 00:00:00
abstract::T cell receptor (TCR) genes are initially activated in hematopoietic stem cells that have been committed to the T cell lineage during ontogeny. We have established cell lines derived from hematopoietic organs which express truncated TCR delta mRNA (1.3 kbp). In this study, cDNA clones of this mRNA were analyzed to cha...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250611
更新日期:1995-06-01 00:00:00
abstract::Immunodominant T cell epitopes from the autoantigen human cartilage glycoprotein 39 have previously been mapped in the context of HLA-DR*0401 and *0402, using mice expressing HLA-DR4 transgenes. We measured the dissociation rates of these epitopes from soluble recombinant DR*0401 and DR*0402 to assess the relationship...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200203)32:3<662::AID-IMMU662>3.0
更新日期:2002-03-01 00:00:00
abstract::Naive T cells do not proliferate but remain alive in vivo. In contrast, naive T cells rapidly die in an in vitro culture, suggesting that some factors that are present at the sites of naive T cell circulation in vivo but missing in the bovine serum-containing culture medium, are necessary for their survival. The prese...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425340
更新日期:2005-01-01 00:00:00
abstract::MS and EAE are T cell-driven autoimmune diseases of the CNS where IL-17-producing Th17 cells promote damage and are pathogenic. Conversely, tolerogenic DCs induce Treg cells and suppress Th17 cells. Chloroquine (CQ) suppresses EAE through the modulation of DCs by unknown mechanisms. Here, we show that STAT 1 is necess...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747362
更新日期:2018-07-01 00:00:00
abstract::We have analyzed the inducibility of protein kinase C (PKC)-dependent expression of CD 69 molecules in T cell receptor (TCR) transgenic thymocytes developing in the presence or absence of selecting, class I major histocompatibility complex (MHC) molecules. Small CD4+8+ thymocytes developing in the absence of selecting...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230326
更新日期:1993-03-01 00:00:00
abstract::IFN-gamma plays a critical role in protection against Bordetella pertussis, but Th1 cells are only detectable after the infection has started to resolve, suggesting a protective role for innate IFN-gamma early in infection. Here, we demonstrate significant recruitment of NK cells and NKT cells into the lungs following...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425092
更新日期:2004-09-01 00:00:00
abstract::Stimulation of T cells via the T cell receptor (TCR) leads to an increase intracellular in free Ca2+ levels ([Ca2+]i) and the activation of the MAP kinase signaling pathway. This study analyzes for the first time Ca2+ fluxes in naive cytotoxic T cells stimulated with full agonists, partial agonists, or antagonists. Fo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271241
更新日期:1997-12-01 00:00:00
abstract::Sand fly saliva contains maxadilan, a peptide that causes vasodilation and modifies the secretion of pro-inflammatory cytokines by macrophages. We show that 1 to 10 microg maxadilan protected BALB/c mice against a lethal dose of LPS. Maxadilan reduced serum levels of TNF-alpha by approximately tenfold, while it caused...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199810)28:10<3120::AID-IMM
更新日期:1998-10-01 00:00:00
abstract::Th cells can adopt a number of different phenotypes. We performed microarray-assisted mRNA profiling on antigen-stimulated, TCR transgenic murine splenocytes that were cultured in the presence of cytokines. Transcriptome snapshots of Th cells differentiating into Th1 and Th2 phenotypes were obtained at various time po...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242979
更新日期:2013-04-01 00:00:00
abstract::Transcription of the gene encoding the transcriptional coactivator Oct-binding factor 1 (OBF-1)/OCA-B/Bob.1 is largely restricted to B cells. During B cell development OBF-1 expression shows two peaks, one in immature B cells in the bone marrow and the other in germinal center B cells. Promoter analysis has identified...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323882
更新日期:2003-10-01 00:00:00
abstract::To study the question when and where self peptides become associated with major histocompatibility complex class II molecules for tolerance induction, we recently developed a system in which the intracellular site(s) of antigen expression could be manipulated using gene cloning techniques. We previously constructed a ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210613
更新日期:1991-06-01 00:00:00
abstract::Platelet-activating factor (PAF) is a phospholipid mediator of anaphylaxis, released from basophils of several mammalian species, that aggregates platelets and releases their vasoactive amines. The ionophore A23 187 induced the release of PAF from rat and mouse peritoneal cells, a mixed cell population that was fracti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090512
更新日期:1979-05-01 00:00:00
abstract::The proliferative state of functionally defined populations of size-separated pre-B cells was investigated. The results presented here support the hypothesis that pre-B cells are in the G2 phase when stable membrane IgM is first expressed. Small resting precursor cells significantly adhere to nylon wool columns, unlik...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830120406
更新日期:1982-04-01 00:00:00
abstract::The liver and activation-regulated chemokine (LARC), also termed MIP-3alpha and Exodus, is a novel human CC chemokine with a selective chemotactic activity for lymphocytes and dendritic cells. Here we describe genomic and cDNA clones encoding the murine orthologue of LARC (mLARC). The gene consists of four exons and t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199902)29:02<633::AID-IMMU
更新日期:1999-02-01 00:00:00
abstract::The molecular mechanisms leading to anti-double-stranded (ds) DNA antibody production in systemic lupus erythematosus (SLE) are poorly understood. We describe here the immunoglobulin variable region genes of six human hybridomas secreting IgG anti-dsDNA antibodies derived from three SLE patients. The monoclonal IgG an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220709
更新日期:1992-07-01 00:00:00