Abstract:
:To study the question when and where self peptides become associated with major histocompatibility complex class II molecules for tolerance induction, we recently developed a system in which the intracellular site(s) of antigen expression could be manipulated using gene cloning techniques. We previously constructed a truncated IgGa gene comprising a variable (V) domain and the CH3 domain (not including the membrane exons) from the IgG2ab heavy (H) chain. The secreted form of the V-CH3b protein was expressed at high levels under control of the Ig H chain enhancer in Ia+ B lymphoma cells and was efficiently recognized by class II-restricted IgG2ab-specific T cell hybrids. Here we describe a modified V-CH3b gene construct in which the sequences encoding the signal peptide were deleted. A strong argument can be made that the signal-less V-CH3b protein is predominantly expressed in the cytosol. We show that transfected L cell lines expressing the signal-less form of the V-CH3b protein can stimulate class II-restricted IgG2ab-specific T cells. Cell mixing experiments indicate that this response cannot be due to passive uptake of soluble antigenic peptides released into culture supernatants. These experiments demonstrate that a cytoplasmic protein having no obvious means of reaching the cell surface can be presented to class II-restricted T cells.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Bikoff EKdoi
10.1002/eji.1830210613subject
Has Abstractpub_date
1991-06-01 00:00:00pages
1411-7issue
6eissn
0014-2980issn
1521-4141journal_volume
21pub_type
杂志文章abstract::Similar idiotopes are detected on anti-allotypic antibodies synthesized by all rabbits immunized against the b6 allotype. The expression of similar idiotypes on anti-b6 antibodies has been found in rabbits of all VHa allotypic types thus far studies. For their expression, these idiotypes require the interaction betwee...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110606
更新日期:1981-06-01 00:00:00
abstract::The receptor for the inflammatory peptide C3a has scarcely been examined on human cells. This work demonstrates that human tumor-derived basophilic granulocytes express C3a receptors, and presents parts of the hitherto unknown C3a-signal transduction. When incubated with IL-3, these cells specifically liberated histam...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230239
更新日期:1993-02-01 00:00:00
abstract::Membrane-coated microvesicles (MVs) have been identified as important mediators in intercellular communication. During the process of apoptosis, dying cells dynamically release MVs. Neutrophils are the most abundant type of leukocytes in the circulation. Due to their very short lifespan, it is likely that they are the...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201546203
更新日期:2017-05-01 00:00:00
abstract::Dendritic cells (DC) are known to support the activation of natural killer (NK) cells. However, little is known about the role for DC in NK-cell homeostasis. In order to investigate this question, a novel bacterial artificial chromosome transgenic mouse model was generated in which the diphtheria toxin receptor is exp...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838659
更新日期:2008-10-01 00:00:00
abstract::We previously showed that granulocyte-macrophage colony-stimulating factor (GM-CSF) breaks tolerance induction. The objective of this study was to determine whether GM-CSF breaks established inhalation tolerance. To induce tolerance, BALB/c mice were exposed to aerosolized ovalbumin (OVA) for 10 consecutive days. A co...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425012
更新日期:2004-09-01 00:00:00
abstract::Single-positive thymocytes are the immediate precursors of peripheral recent thymic emigrants (RTE) which develop into mature peripheral T cells. The functional ability of RTE is unclear but their state of differentiation may be relevant to the development of tolerance to peripheral "self" antigens. Since RTE are diff...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240738
更新日期:1994-07-01 00:00:00
abstract::Riboflavin, also known as vitamin B2 , is converted by riboflavin kinase (RFK) into flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which are essential cofactors of dehydrogenases, reductases, and oxidases including the phagocytic NADPH oxidase 2 (Nox2). Riboflavin deficiency is common in young adul...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343940
更新日期:2014-03-01 00:00:00
abstract::Chemokines are chemotactic cytokines with a key role in the control of cell trafficking and positioning under homeostatic and inflammatory conditions. D6 is a promiscuous 7-transmembrane-domain receptor expressed on lymphatic vessels which recognizes most inflammatory, but not homeostatic, CC chemokines. In vitro expe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200526114
更新日期:2005-05-01 00:00:00
abstract::Interleukin (IL)-5 is produced by variety of cell types and contributes to both lymphocyte development an eosinophil terminal differentiation in vitro. The coincidence of worm expulsion and eosinophilia in sheep infected with the gastrointestinal nematode Trichostrongylus colubriformis suggest that eosinophils may be ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260308
更新日期:1996-03-01 00:00:00
abstract::The crucial role of interleukin (IL)-4 in the induction of murine IgG1 and IgE responses, which are coupled through the process of sequential isotype switching, has been well documented. Whereas IL-4 is obligatory for the induction of IgE responses, it enhances IgG1 responses. In this study, using neutralizing antibod...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240624
更新日期:1994-06-01 00:00:00
abstract::Responsiveness to IL-13 involves at least two chains, IL-4Ralpha and IL-13Ralpha1. Although mouse B cells express IL-4Ralpha, little is known about their expression of IL-13Ralpha chains. To investigate this topic further, we have generated a monoclonal antibody (C41) specific for murine IL-13Ralpha1. Using C41, IL-13...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200011)30:11<3157::AID-IMMU3157>
更新日期:2000-11-01 00:00:00
abstract::Alum has been the only adjuvant licensed for human vaccines for decades and is still widely used, but its mechanism of action remains obscure. Recently, the NLRP3 inflammasome has been linked to the immunostimulatory properties of alum and other particulate adjuvants, although it is disputed to what degree NLRP3 is ge...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.200940039
更新日期:2010-03-01 00:00:00
abstract::The responses of a single cloned T cell line to three different class II major histocompatibility complex (MHC) ligands have been compared for avidity, determined by inhibition with anti-T cell receptor Fab fragments directed at two different receptor epitopes, and for ease of inhibition with anti-CD4 antibody. It has...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190114
更新日期:1989-01-01 00:00:00
abstract::We have identified a novel subset of early B lineage cells in the mouse bone marrow (BM) by GL7 expression on cell surface. GL7(+)B220(low) BM cells have a large cell size and are CD43(-to low), CD95(-), Sca-1(-), I-A(low), IgM(-) and IgD(-), suggesting that they are large pre-B cells. These BM cells express lambda5 a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200201)32:1<291::AID-IMMU291>3.0
更新日期:2002-01-01 00:00:00
abstract::The extent of emigration of cells from the bursa of Fabricius to the periphery was estimated. Per anum application of fluorescein isothiocyanate to label bursal cells in situ was used. Migrant cells can be visualized on frozen sections or cell suspensions of peripheral organs by their fluorescence. The data show that ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190527
更新日期:1989-05-01 00:00:00
abstract::A number of applications of antibodies in diagnosis and therapy require multivalent reagents either because of the polymeric nature of the antigens or because biological activity depends on an effect on the formation of homodimeric species. Here, we report a procedure for mass screening of phage-derived monomeric anti...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270307
更新日期:1997-03-01 00:00:00
abstract::Staphylococcus aureus is the main pathogen associated with septic arthritis. Upon infection, neutrophils are quickly recruited to the joint by different chemoattractants, especially CXCR1/2 binding chemokines. Although their excessive accumulation is associated with intense pain and permanent articular damage, neutrop...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747198
更新日期:2018-03-01 00:00:00
abstract::By comparing the restriction patterns of the DNA from 23 unrelated individuals 16 polymorphisms were defined which allowed us to differentiate between the duplicated copies Op, Ap, Lp and Od, Ad, Ld of the kappa locus (p for the C kappa proximal, d for the distal copy). Some of these duplication-differentiating polymo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210808
更新日期:1991-08-01 00:00:00
abstract::CD4+ CD25+ Foxp3+ regulatory T cells (Treg) are natural suppressors of autoimmunity but they can also dampen the effective clearance of infectious organisms. These cells have the potential to be exploited to prevent transplant rejection and to treat autoimmune disease. A paper in this issue of the European Journal of ...
journal_title:European journal of immunology
pub_type: 评论,杂志文章,评审
doi:10.1002/eji.200635967
更新日期:2006-04-01 00:00:00
abstract::The expression and action of Fas/Fas ligand (FasL) in multiple sclerosis has been postulated as a major pathway leading to inflammatory demyelination. To formally test this hypothesis, C57BL/6-lpr and -gld mice, which due to gene mutation express Fas and FasL in an inactive form, were immunized with myelin oligodendro...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271211
更新日期:1997-12-01 00:00:00
abstract::The MRC OX-47 monoclonal antibody recognizes a membrane antigen present at low levels on many lymphocytes but whose expression is markedly increased on activation with mitogens. cDNA clones for the OX-47 antigen were isolated from an expression library and the protein sequence deduced. It contains a leader sequence gi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210320
更新日期:1991-03-01 00:00:00
abstract::MHC class I molecules are important components of immune surveillance. There are no available methods to directly visualize and determine the quantity and distribution of MHC/peptide complexes on individual cells or to detect such complexes on antigen-presenting cells in tissues. MHC-restricted recombinant antibodies ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737524
更新日期:2008-06-01 00:00:00
abstract::Amount and affinity of anti-beta-D-galactosidase-activating antibodies (AAb) were studied in two lines of mice obtained by selective breeding for antibody response to heterologous erythrocytes. These lines, referred to as high (H) and low (L) lines, differ also in their responses to many antigens. Concerning AAb amoun...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090312
更新日期:1979-03-01 00:00:00
abstract::The effect of cyclosporin A (CsA) on the delayed-type hypersensitivity (DTH) elicited in mice by sheep red blood cells was investigated. Evidence is presented that a single injection of CsA adversely affects the inflammatory reaction. Sensitized T lymphocytes initiate the DTH reaction by their recruiting activity on p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140407
更新日期:1984-04-01 00:00:00
abstract::Pathogens have developed a plethora of strategies to undermine host immune defenses in order to guarantee their survival. For large DNA viruses, these immune evasion mechanisms frequently rely on the expression of genes acquired from host genomes. Horizontally transferred genes include members of the immunoglobulin su...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201746984
更新日期:2017-05-01 00:00:00
abstract::Delayed hypersensitivity (DH) reaction to human Ig, corynebacterium parvum or allogeneic cells at the site of tumor cell injection, suppressed the fibrosarcoma (SCFS) growth in chickens. Spleen cells of SC chickens sensitized to human Ig or C. parvum suppressed SCFS growth when adoptively transferred with tumor cells,...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830080408
更新日期:1978-04-01 00:00:00
abstract::gp140 is the Epstein-Barr virus receptor and the C3d receptor (EBVR/C3dR) of human B lymphocytes. Recently, we have shown that cross-linking of EBVR/C3dR on cell surface by polyclonal anti-gp140 induced B cell activation, in presence of T cell factors. Immunoregulatory abnormalities of EBV-induced B cell activation ha...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830161108
更新日期:1986-11-01 00:00:00
abstract::The immune and endocrine systems ensure two vital functions in the body. The immune system protects us from lethal pathogens, whereas the endocrine system ensures proper metabolic function of peripheral organs by regulating systemic homeostasis. These two systems were long thought to operate independently. The immune ...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201847895
更新日期:2019-07-01 00:00:00
abstract::IL-33 is a novel multi-functional IL-1 family member that, in contrast to other family members, is associated with Th2 responses. IL-33 signals via a heterodimer composed of its receptor, IL-1 receptor-like-1 (IL-1RL1), more commonly known as ST2L, and the IL-1R accessory protein. ST2L is expressed by endothelial cell...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.200940026
更新日期:2009-12-01 00:00:00
abstract::Antigen stimulation induces specific CD4 T cells to change from a resting phenotype (CD45RC+) to a "memory" phenotype (CD45RC-), an isoform switch that is reversible and regulated by persisting antigen. We show here that CD4 T cells responsible for mediating allograft rejection undergo a similar CD45RC+ to CD45RC- swi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199907)29:07<2177::AID-IMM
更新日期:1999-07-01 00:00:00