Abstract:
:The MRC OX-47 monoclonal antibody recognizes a membrane antigen present at low levels on many lymphocytes but whose expression is markedly increased on activation with mitogens. cDNA clones for the OX-47 antigen were isolated from an expression library and the protein sequence deduced. It contains a leader sequence giving a mature protein of 251 amino acids with a single putative transmembrane region, a cytoplasmic domain of 40 amino acids and an extracellular domain of 187 amino acids that contained two immunoglobulin-like domains. The putative transmembrane sequence includes a glutamic acid residue within the hydrophobic sequence. The presence of acidic residues within the hydrophobic sequence of transmembrane sequences usually indicates association with other polypeptides and this is predicted for the OX-47 antigen. A sequence of 37 amino acids that included all the transmembrane region was identical to that of the chicken HT7 antigen present on endothelium in brain and erythroblasts. The level of protein sequence identity in the Ig-like domains was lower but HT7 is almost certainly the chicken homologue of the rat OX-47 antigen. The ligand and function of the molecule are unknown. In addition to lymphoblasts the OX-47 antigen was localized on a variety of other cell types including various immature cells, endothelia and cells with excitable membranes.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Fossum S,Mallett S,Barclay ANdoi
10.1002/eji.1830210320subject
Has Abstractpub_date
1991-03-01 00:00:00pages
671-9issue
3eissn
0014-2980issn
1521-4141journal_volume
21pub_type
杂志文章abstract::The antibody response to the synthetic polypeptide, poly(LTyr, LGlu)-poly(DLAla)--poly(LLys), [(T,G)-A--L], injected entrapped in liposomes which served as adjuvant has been analyzed. The liposomes used were composed of phosphatidylcholine, cholesterol, dicetylphosphate and DL alpha-tocopherol (molar ratios as 4:3:0.1...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110510
更新日期:1981-05-01 00:00:00
abstract::Biopsy specimens were obtained from the bronchial or the nasal mucosa of three patients with grass pollen-induced bronchial asthma or rhinitis 48 h after positive bronchial or nasal provocation test with grass pollen extract. T cell clones (TCC), derived from these and control specimens, were then assessed for their p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230707
更新日期:1993-07-01 00:00:00
abstract::The glycoprotein CD8 is believed to play an important role in the maturation and function of MHC class I-restricted T lymphocytes. CD8 has been proposed to function as a co-receptor of the TcR to participate in signal transduction, possibly through its cytoplasmic domain that binds to protein tyrosine kinase p56lck. A...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231117
更新日期:1993-11-01 00:00:00
abstract::Mouse bone marrow cells differentiate in culture in the presence of L cell-conditioned medium to macrophages (M phi). Proliferation, release of plasminogen activator, expression of transglutaminase, random motility and response to a standard preparation of purified M phi migration inhibitory factor (MIF) was recorded ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830141008
更新日期:1984-10-01 00:00:00
abstract::We have studied a secondary IgG anti-dinitrophenyl response in dissociated mouse spleen cell cultures which is comparable in magnitude to the responses detected after adoptive transfer. The data indicate that the responsive precursor cell is not a recirculating memory cell but is an intermediate B cell type which appe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060104
更新日期:1976-01-01 00:00:00
abstract::In experimental autoimmune encephalomyelitis (EAE), intravenous transfer of activated CD4(+) myelin-specific T cells is sufficient to induce disease. Transferred T cells access the CNS parenchyma by trafficking across the blood brain barrier (BBB) vascular endothelium into the perivascular space, and then across the g...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425864
更新日期:2005-04-01 00:00:00
abstract::We have investigated perforin and granzyme B expression in graft-infiltrating lymphocytes of patients who underwent heart transplantation. Those proteins are commonly present in the cytoplasmic granules of cytotoxic T lymphocytes and are released upon effector-target cell interaction. From 28 patients 103 endomyocardi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240136
更新日期:1994-01-01 00:00:00
abstract::The monoclonal antibody HC1/6 generated against phorbol 12-myristate 13-acetate-treated U-937 cells recognizes a new cell surface antigen with a broad relative molecular mass ranging from 100 to 150 kDa. This antigen is also present on monocytes, platelets and endothelial cells and is weakly expressed by granulocytes....
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190804
更新日期:1989-08-01 00:00:00
abstract::Mice deficient for the adapter protein SLP65 (BLNK) show a partial block in early B cell development, reduced numbers of mature B cells in the periphery, an absence of B1 cells and a reduction of IgM and IgG3 serum immunoglobulin levels. A strikingly similar phenotype is observed in Btk-deficient mice. To investigate ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200107)31:7<2164::aid-immu2164>3
更新日期:2001-07-01 00:00:00
abstract::Recent studies have shown that in humans the germinal center reactions produce three types of V(D)J mutated B cells in similar proportions, i.e. Ig-switched, IgD-IgM+ (IgM-only) and IgD+IgM+ cells, and that together they form the CD27+ compartment of recirculating B cells. We investigated the Ig isotype switch capacit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200101)31:1<243::AID-IMMU243>3.0
更新日期:2001-01-01 00:00:00
abstract::Changes in the epigenetic landscape of immune cells are a crucial component of gene activation during the induction of inflammatory responses, therefore it has been hypothesized that epigenetic modulation could be employed to restore homeostasis in inflammatory scenarios. Fungal pathogens cause a large burden of morbi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201848081
更新日期:2019-11-01 00:00:00
abstract::To elucidate the rapid destruction of allogeneic bone marrow (BM) grafts in nonsensitized rats we have assessed: (a) the 21-h tissue localization of 51Cr-labeled allogeneic vs. syngeneic BM cells, (b) the ability of allogeneic BM cells to proliferate in the BM or irradiated recipients, (c) the survival of allogeneic v...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140906
更新日期:1984-09-01 00:00:00
abstract::Beside diabetes, non-obese diabetic (NOD) mice develop sporadic lymphoid infiltration of the thyroid gland, mimicking Hashimoto's thyroiditis. We have examined the prevalence of those manifestations in NOD mice, the influence of the major histocompatibility complex (MHC) and the association with autoantibodies. The in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271117
更新日期:1997-11-01 00:00:00
abstract::After incorporation of 14C-labeled amino acids and/or of [3H]fucose during in vitro culture of the Thy-1.1-bearing, Fc receptor-positive T lymphoma, L-5187-Y, attempts were made to purify the Fc binding structure(s). Following solubilization of a crude membrane pellet using sodium deoxycholate, the 110 000 x g superna...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830070916
更新日期:1977-09-01 00:00:00
abstract::Vaccinia virus (VV)-encoded MHC class I Kd molecules which differ by a single amino acid change from glutamine (Kdw, wild type) to histidine (Kdm, mutant) at position 114 located in the floor of the peptide binding groove were compared in terms of peptide binding and cytotoxic T (Tc) cell recognition. Most anti-viral ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199904)29:04<1228::AID-IMM
更新日期:1999-04-01 00:00:00
abstract::Restriction fragment length polymorphism has been compared between the Igh loci of C57BL/6 and MOLF/EI (Mus musculus molossinus) mice utilizing probes which detect the C gamma 2b gene and genes from nine VH-gene families. Distinct restriction site patterns were found for the CH genes and for VH families PC7183, Q52 an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170920
更新日期:1987-09-01 00:00:00
abstract::Continuous antigen stimulation of CD4(+)CD25(-) T cells leads to generation of adaptive CD4(+)CD25(+)FOXP3(+) regulatory T (T(R)) cells. Here, we show that highly suppressive adaptive CD8(+)CD25(+)FOXP3(+) T cells can be generated in the same manner by continuous antigen stimulation in the presence of CD14(+) monocyte...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737529
更新日期:2008-03-01 00:00:00
abstract::Monoclonal antibodies (mAb) derived from BALB/c mice immunized with human anti-HLA-A2 cloned cytotoxic T lymphocytes (CTL) were screened for their ability to block, in the absence of complement, the cytolytic activity of the immunizing CTL clone. Eight cytolysis-inhibiting mAb have been derived. One of these was direc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830120908
更新日期:1982-09-01 00:00:00
abstract::Mercuric chloride (HgCl2) has contrasting effects on different rat strains: susceptible strains, e.g. Brown Norway (BN) develop polyclonal B cell activation, multiple autoantibodies and widespread tissue injury. Lewis (LEW) rats are resistant: no autoimmune response occurs after HgCl2; instead, there is immunosuppress...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261018
更新日期:1996-10-01 00:00:00
abstract::By comparing the restriction patterns of the DNA from 23 unrelated individuals 16 polymorphisms were defined which allowed us to differentiate between the duplicated copies Op, Ap, Lp and Od, Ad, Ld of the kappa locus (p for the C kappa proximal, d for the distal copy). Some of these duplication-differentiating polymo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210808
更新日期:1991-08-01 00:00:00
abstract::Activated monocyte-macrophages have been implicated in tumor angiogenesis via their capacity to produce many potent angiogenic factors. However, the mechanisms leading to production of these angiogenic factors in macrophages remain to be elucidated. In this study, we demonstrated by use of a mouse Matrigel implantatio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200424957
更新日期:2004-08-01 00:00:00
abstract::Collagen type II-induced arthritis (CIA) is an experimental model of arthritis that has been successfully used to dissect the pathogenesis of human rheumatoid arthritis and to identify potential therapeutic targets. We have used this model to evaluate the role of T cell co-stimulation in both disease development and p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261008
更新日期:1996-10-01 00:00:00
abstract::High-dose infusion of IgG (IVIG) is used to treat autoimmune and inflammatory diseases, including Kawasaki disease (KD). Although the immunomodulatory effects of IVIG on blood cells such as macrophages have been well studied, its effects on tissue cells remain unclear. Here, we show that high-dose IgG specifically and...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242398
更新日期:2012-08-01 00:00:00
abstract::Two cloned murine helper T lymphocyte (HTL) lines were used to investigate the immunoregulatory properties of highly purified interleukin 2 (IL2). The clone, designated J6.19, secretes lymphokines, including IL2, and proliferates when stimulated with ovalbumin in the presence of I-Ak-bearing spleen cells, while the al...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160302
更新日期:1986-03-01 00:00:00
abstract::Using a specific antibody, we found that expression of the viral restriction factor IFITM3 differs across cell types within the immune compartment with higher expression in myeloid rather than lymphoid cells. IFITM3 expression was increased following IFN stimulation, mostly type I, in immune cells, with the exception ...
journal_title:European journal of immunology
pub_type: 信件
doi:10.1002/eji.202048706
更新日期:2020-10-30 00:00:00
abstract::In previous publications it was shown by avidity measurements, cross-reactivity patterns and genetic analyses, that the tetrapeptide T-T-G-G is the immuno-dominant epitope of the synthetic polypeptide (T, G)-A--L. In the present study this close immunological relationship between the random multichain copolymer (T, G)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060905
更新日期:1976-09-01 00:00:00
abstract::Self-glycosphingolipids bind to surface CD1 molecules and are readily displaced by other CD1 ligands. This capacity to exchange antigens at the cell surface is not common to other antigen-presenting molecules and its physiological importance is unclear. Here we show that a large pool of cell-surface CD1a, but not CD1b...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535544
更新日期:2006-05-01 00:00:00
abstract::Glycosylphosphatidylinositol-anchored (GPI)-Db molecules are defective in mediating cytotoxic T lymphocytes (CTL) lysis of transfected lymphoma cells, compared to their transmembrane (TM) counterpart. This defect is manifest when antigenic peptide must be processed and presented through the endogenous pathway. These s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260938
更新日期:1996-09-01 00:00:00
abstract::Anti-idiotypes produced against monoclonal anti-H-2 antibodies have been used to examine idiotope sharing among a panel of anti-major histocompatibility complex (MHC) antibodies detecting the same and different specificities. Both xenogeneic anti-100-30 and anti-3-83, which did not react with predominant idiotypes in ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130611
更新日期:1983-06-01 00:00:00
abstract::Dermal and mucosal lymphocyte infiltrates in patients with lichen ruber planus and chronic graft-vs.-host disease exhibited reactivity with anti-fibronectin antibodies. This reactivity was associated with the lymphocytes and localized pericellularly. In contrast, biopsy areas outside lymphocyte accumulations were rela...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210209
更新日期:1991-02-01 00:00:00