Abstract:
:Self-glycosphingolipids bind to surface CD1 molecules and are readily displaced by other CD1 ligands. This capacity to exchange antigens at the cell surface is not common to other antigen-presenting molecules and its physiological importance is unclear. Here we show that a large pool of cell-surface CD1a, but not CD1b molecules, is stabilized by exogenous lipids present in serum. Under serum deprivation CD1a molecules are altered and functionally inactive, as they are unable to present lipid antigens to T cells. Glycosphingolipids and phospholipids bind to, and restore functionality to CD1a without the contribution of newly synthesized and recycling CD1a molecules. The dependence of CD1a stability on exogenous lipids is not related to its intracellular traffic and rather to its antigen-binding pockets. These results indicate a functional dichotomy between CD1a and CD1b molecules and provide new information on how the lipid antigenic repertoire is immunologically sampled.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Manolova V,Kistowska M,Paoletti S,Baltariu GM,Bausinger H,Hanau D,Mori L,De Libero Gdoi
10.1002/eji.200535544subject
Has Abstractpub_date
2006-05-01 00:00:00pages
1083-92issue
5eissn
0014-2980issn
1521-4141journal_volume
36pub_type
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