Abstract:
:In this investigation we compare the repopulation of the CD8+ T cell compartments of bone marrow (BM) chimeras by either normal nontransgenic or T cell receptor (TcR) alpha beta-transgenic (TG) CD8+ T cells, the fate of TG and non-TG CD8+ T cells in different parabionts and the survival of TG and non-TG peripheral CD8+ T cells after transfer into athymic hosts. We found that cellular competition among CD8 T cells occurs at several steps of T cell differentiation including a) during the DN to DP transition, b) positive selection in the thymus, c) export from the thymus and d) in the periphery. Comparison of the results obtained in the BM chimeras and in the parabionts shows that an important step of T cell selection occurs during seeding of peripheral lymphoid tissues. Once established, peripheral T cells resist replacement by recent thymus migrants, i.e. in the periphery, selection of T cell repertoires follows the rule "first come, first served". Peripheral dominance correlates with T cell activation and division. Cell cycling and CD44 expression are more frequent among non-TG CD8 T cells than TG CD8 T cells and within the latter, more frequent among P14 TG CD8 T cells than anti-HYTG CD8 T cells. Thus, in the absence of intentional immunization, the frequencies of CD8+ T cells follow a hierarchy of selection in which non-TG > or = P14 TG > anti-HY TG. We also show that the equilibrium size and the fate of one CD8 T cell population differs according to the presence or absence of other CD8 T cell populations. Under these circumstances, selection of T cell repertoires and T cell survival and memory rely not only on the interactions of each T cell with their respective ligands, but also on the nature and number of other competing cells.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Freitas AA,Agenes F,Coutinho GCdoi
10.1002/eji.1830261115subject
Has Abstractpub_date
1996-11-01 00:00:00pages
2640-9issue
11eissn
0014-2980issn
1521-4141journal_volume
26pub_type
杂志文章abstract::Human herpesvirus 6B (HHV-6B) is a ubiquitous pathogen with frequent reactivation observed in immunocompromised patients such as BM transplant (BMT) recipients. Adoptive immunotherapy is a promising therapeutic avenue for the treatment of opportunistic infections, including herpesviruses. While T-cell immunotherapy ca...
journal_title:European journal of immunology
pub_type: 临床试验,杂志文章
doi:10.1002/eji.201444931
更新日期:2014-12-01 00:00:00
abstract::This report describes the study of the expression of an idiotype in the human population which is associated with antibodies to N-acetyl-D-glucosamine (GlcNAc) present in most human sera presumably due to streptococcal infections. The idiotype is identified with antisera and monoclonal antibodies prepared against the ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130402
更新日期:1983-04-01 00:00:00
abstract::The murine T cell clone D10.G4.1 can be induced to proliferate by monoclonal antibodies (mAb) to the T cell receptor (TcR) or to Thy-1 molecules. When cells were stimulated by anti-TcR mAb, a group of 4 proteins (19-25 kDa) was specifically phosphorylated. This effect was completely mimicked by the Ca2+ ionophore A231...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180727
更新日期:1988-07-01 00:00:00
abstract::Adult, male Lewis rats received a single injection of 5-bromo-2'-deoxyuridine (BrdUrd) i.v. to label proliferating cells in the S phase of the cell cycle. After 1 and 24 h the thymus, bone marrow, blood, spleen, peripheral, cervical and mesenteric lymph nodes as well as Peyer's patches were removed. In cell suspension...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190619
更新日期:1989-06-01 00:00:00
abstract::In the present study, we examined whether anti-CD40+IL-4-mediated B cell proliferation and immunoglobulin synthesis is affected by vitamin D (VD) and its low-hypercalcemic analogue EB1089 in Bcells from healthy donors. Analysis of vitamin D receptor (VDR) expression showed that only anti-CD40+IL-4-stimulated, but not ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200212)32:12<3395::AID-IMMU3395>
更新日期:2002-12-01 00:00:00
abstract::As discussed in this review, human cytomegalovirus (HCMV) infection in healthy individuals is associated with a variable and persistent increase of NK cells expressing the CD94/NKG2C activating receptor. The expansion of NKG2C(+) NK cells reported in other infectious diseases is systematically associated with HCMV co-...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201243117
更新日期:2013-05-01 00:00:00
abstract::We previously showed that topical flagellin induces profound mucosal innate protection in the cornea against microbial infection, a response involving multiple genes and cell types. In this study, we used a Candida albicans (CA)-C57BL/6 mouse keratitis model to delineate the contribution of CXCL10- and CXCR3-expressin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201444490
更新日期:2014-09-01 00:00:00
abstract::In the bone marrow (BM), memory plasma cells (PCs) survive for long time periods in dedicated microenvironmental survival niches, resting in terms of proliferation. Several cell types, such as eosinophils and reticular stromal cells, have been reported to contribute to the survival niche of memory PCs. However, until ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201344313
更新日期:2014-08-01 00:00:00
abstract::An anti-T cell receptor (TcR) monoclonal antibody (mAb), LC4, directed against a human leukemic T cell line, SUP-T13, caused DNA fragmentation ("apoptosis") and cell death upon binding to this cell line. Cross-linking of receptor molecules was necessary for this effect since F(ab')2, but not Fab', fragments of LC4 cou...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191023
更新日期:1989-10-01 00:00:00
abstract::There are no direct means to study class I MHC presentation in human normal or diseased cells. Using CMV-infected human cells and applying novel mAb that mimic T-cell receptor specificity directed toward the immunogenic epitope of the viral pp65 protein presented on HLA-A2 molecules, we directly imaged the dynamics of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939875
更新日期:2010-06-01 00:00:00
abstract::Protein kinase C (PKC) has been implicated in the signaling of a number of cellular responses including activation of T cells. In the present report we have evaluated the effect of increased cAMP levels on PKC activation after stimulation of two distinct receptor systems on normal human T cells. PKC substrate phosphor...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190622
更新日期:1989-06-01 00:00:00
abstract::Rat CD4+ T cells are divided phenotypically by the anti-CD45RC monoclonal antibody OX22 into subsets with contrasting functions. Stimulation of T cells in vitro is known to induce a change in isoform from CD45RC+ to CD45RC-. We have investigated the in vitro conditions which promote a switch in isoform in the opposite...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230117
更新日期:1993-01-01 00:00:00
abstract::Although anti-inflammatory properties of glucocorticoids (GC) are well documented, their activity in allergic diseases is still controversial. Recently, it has been reported that GC can increase, both in vivo and in vitro, the polyclonal production of total IgE. In this study we investigated the effects of GC on the a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270933
更新日期:1997-09-01 00:00:00
abstract::High frequencies of CD5+TcR alpha/beta- T cells were induced in the peritoneal cavity of rats immunized with syngeneic W439 lymphoma cells. These TcR alpha/beta- cells expressed TcR delta mRNA as analyzed by the polymerase chain reaction technique. The delta + (TcR gamma/delta +) T cells were of the CD2+, CD3+, CD4-, ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830211122
更新日期:1991-11-01 00:00:00
abstract::Although originally described in tumor-bearing hosts, myeloid-derived suppressor cells (MDSC) have been detected under numerous pathological situations that cause enhanced demand of myeloid cells. Thus, MDSC might be part of a conserved response to different endogenous and exogenous stress signals, including inflammat...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.200939892
更新日期:2009-10-01 00:00:00
abstract::The T cell repertoire of BALB/c mice contains clones capable of recognizing p-azobenzenearsonate (ABA)-tyrosine (Tyr) in association with both I-A and I-E-encoded class II molecules. Immunization of BALB/c animals with ABA-GAT (terpolymer of L-Glu60-L-Ala30-L-Tyr10) or ABA-GLT (terpolymer of L-Glu51-L-Lys34-L-Tyr15) i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830151214
更新日期:1985-12-01 00:00:00
abstract::Analysis of mice transgenic for immunoglobulin genes should allow definition of the cis-acting DNA sequences required to target somatic mutation to antibody V genes. We have looked for mutations in a chimeric kappa transgene encoding a V region specific for the hapten 2-phenyloxazolone (phOx) linked to a rat C kappa g...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200625
更新日期:1990-06-01 00:00:00
abstract::Alkaline phosphatases (APases, EC 3.1.3.1) are ecto-enzymes bound to cell membranes by a phosphatidyl-inositol anchor. We have previously shown that APase is present on activated murine B cells and its expression correlates with the process of B cell differentiation into immunoglobulin secretion. Recently, a monoclona...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200436
更新日期:1990-04-01 00:00:00
abstract::Autoantibodies in the form of immune complexes are known to be crucial mediators in initiating inflammation in a variety of autoimmune diseases. This has been well documented in the anti-collagen II antibody-induced arthritis animal model for a long time now. Recently, in the K/B x N mouse model (the F1 of the TCR-tra...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200526167
更新日期:2005-10-01 00:00:00
abstract::The exact identification of B cell subsets is instrumental to understand their dynamics under physiological and pathological conditions. Human memory B cells are currently identified according to the expression of CD27, which is absent on naive B cells. We report here that the ATP-binding cassette (ABC)B1 transporter ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535364
更新日期:2005-12-01 00:00:00
abstract::A survey of KIR2DL4 polymorphism revealed seven common sequences in the Australian population. The seven sequences encode three different amino acid sequences of the immunoglobulin domains. Two of the sequences encoding different amino acid sequences in the immunoglobulin domains also occur on some chromosomes with a ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200201)32:1<18::AID-IMMU18>3.0.C
更新日期:2002-01-01 00:00:00
abstract::Th1 and Th2 cells, which produce distinct sets of cytokines, differentially express several chemokine receptors that may regulate their tissue-specific localization. Although the expression pattern and regulation of chemokines are likely to play a critical role in many immunopathological processes, they remain largely...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(200004)30:4<1030::AID-IMMU
更新日期:2000-04-01 00:00:00
abstract::The present experiments address functional antibody diversity and clonal distribution in murine available repertoires. IgM-containing supernatants were prepared by unbiased, polyclonal stimulation of resting splenic B cells from C57BL/6 mice, to ensure similar numbers of responding clones/culture and equivalent growth...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199804)28:04<1204::AID-IMM
更新日期:1998-04-01 00:00:00
abstract::Immune enhancement is desirable in situations where decreased immunity results in increased morbidity. We investigated whether blocking the surface inhibitory receptor PD-1 and/or p38 MAP kinase could enhance the proliferation of the effector memory CD8(+) T-cell subset that re-expresses CD45RA (EMRA) and exhibits cha...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201445312
更新日期:2015-05-01 00:00:00
abstract::The interaction of CD40 ligand (CD40L) on activated T cells with CD40 on B cells, monocytes and dendritic cells is essential for humoral immunity and for up-regulation of antigen-presenting cell (APC) functions, as a result of signaling through CD40. There are also some indications that after interaction with CD40, CD...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260732
更新日期:1996-07-01 00:00:00
abstract::Dendritic cells (DC) were cultured from mouse bone marrow (BM) progenitors in low concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF) (GM(lo) DC) by two different protocols. The phenotype and functional properties of these GM(lo) DC were compared to those of standard BM-DC cultures generated in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200007)30:7<1813::AID-IMMU1813>3
更新日期:2000-07-01 00:00:00
abstract::The functional half-life, a measure of the persistence of lymphocytes in an antigen-free environment, has been estimated and found to be about 7 days for virgin 2,4,6-trinitrophenyl (TNP)-reactive mouse B cells, and 2--3 times longer for TNP-primed B cells. Using allotype-congenic mice, lymph node cells were transferr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060908
更新日期:1976-09-01 00:00:00
abstract::Mice carrying the lpr gene, SCG and MRL-lpr/lpr mice, were used to characterize the phenotype and lpr gene of abnormally proliferating T cells in these mice. A major population which expanded in these mice were T cells expressing intermediate (int) levels of T cell receptor (TCR) (and CD3) and the phenotype of interle...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260702
更新日期:1996-07-01 00:00:00
abstract::cDNA of the T cell receptor beta (TCRB) have been isolated from the anuran amphibian Xenopus and they show strong structural homology to TCRB sequences of other vertebrates. Ten BV families, two D segments, ten J segments, and a single C region have been defined so far. Each V family consists of one to two members per...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270327
更新日期:1997-03-01 00:00:00
abstract::IL-10 plays an important role in the regulation of immune responses. We and others have demonstrated recently that cyclic adenosine monophosphate (cAMP)-elevating substances up-regulate monocytic IL-10 expression in vitro and in vivo. Computer analysis of the IL-10 promoter/enhancer region localized four putative cAMP...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199910)29:10<3098::AID-IMM
更新日期:1999-10-01 00:00:00