Abstract:
:Analysis of mice transgenic for immunoglobulin genes should allow definition of the cis-acting DNA sequences required to target somatic mutation to antibody V genes. We have looked for mutations in a chimeric kappa transgene encoding a V region specific for the hapten 2-phenyloxazolone (phOx) linked to a rat C kappa gene. Two independent lines of transgenic mice were hyperimmunized with phOx and splenic hybridomas established. In B cells that had been selected by antigen and which used mouse anti-phOx genes, the endogenous sequences were found to be mutated whereas the transgene remained unchanged. These results suggest either that (a) if the transgene is a "passenger" gene expressed at a low level, transgene mutation is a rare event, or that (b) sequences far from the kappa coding region are necessary to direct somatic mutation.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Sharpe MJ,Neuberger M,Pannell R,Surani MA,Milstein Cdoi
10.1002/eji.1830200625subject
Has Abstractpub_date
1990-06-01 00:00:00pages
1379-85issue
6eissn
0014-2980issn
1521-4141journal_volume
20pub_type
杂志文章abstract::Synthetic peptides encoding protective pathogen-derived epitopes represent--in principle--an ideal approach to T-cell vaccination. Empirically, however, these strategies have not been successful. In the current study, we profiled the early activation of CD8+ T cells by MHC class I-restricted peptide immunization to be...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939493
更新日期:2010-01-01 00:00:00
abstract::The role of B cells and antibody in the pathogenesis of experimental autoimmune encephalomyelitis (EAE) remains controversial. We previously demonstrated that B cells are required for EAE to be induced by the 120-amino acid extracellular domain of myelin oligodendrocyte glycoprotein (MOG). In the present study, the ro...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200207)32:7<1905::AID-IMMU1905>3
更新日期:2002-07-01 00:00:00
abstract::We have characterized the peripheral B cell repertoire in T cell-mediated insulin-dependent diabetes mellitus (IDMM) and in B cell-mediated autoimmune idiopathic thrombocytopenic purpura (AITP). The VH6-containing repertoire in adult patients with IDDM or AITP and healthy control subjects was investigated by PCR ampli...
journal_title:European journal of immunology
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更新日期:1999-09-01 00:00:00
abstract::The role of redox regulation in immune-mediated arthritis has been previously described. However, the relationship between innate immune cells, including innate lymphoid cells (ILCs) and phagocyte-derived ROS, in this process remains unclear. Here, we characterize ILCs and measure the IL-1 family cytokines along with ...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2019-11-01 00:00:00
abstract::IL-33 is a novel multi-functional IL-1 family member that, in contrast to other family members, is associated with Th2 responses. IL-33 signals via a heterodimer composed of its receptor, IL-1 receptor-like-1 (IL-1RL1), more commonly known as ST2L, and the IL-1R accessory protein. ST2L is expressed by endothelial cell...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.200940026
更新日期:2009-12-01 00:00:00
abstract::We have proposed earlier that the poor capacity of the lipocalin allergen Bos d 2 to stimulate highly allergic subjects' peripheral blood mononuclear cells could be ascribed to endogenous lipocalins and could be related to the allergenic potential of the molecule. Here, we have characterized the proliferative and cyto...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200322952
更新日期:2003-06-01 00:00:00
abstract::Previously, we have shown that the intravenous (i.v.) injection of allogeneic lymphocytes across an H-2 class I-mutant disparity leads to specific skin allograft tolerance caused by irradiation-sensitive donor T cells, which function as veto cells. In the present study, we show that the i.v. injection of H-2 class II-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200232
更新日期:1990-02-01 00:00:00
abstract::Regulatory T (Treg) cells are critically important for the maintenance of immunological tolerance. Both centrally arising natural nTreg cells and those emerging in the periphery in response to TGF-β, iTreg cells, play a role in the control of unwanted immune responses. Treg cells adopt multiple mechanisms to inhibit e...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142207
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journal_title:European journal of immunology
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更新日期:2008-05-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230636
更新日期:1993-06-01 00:00:00
abstract::A monoclonal antibody (mAb), 3A35, produced against mouse macrophages (M phi) was found to react against certain activated T cells. This mAb, a rat IgM, resulted from a cell fusion between a mouse plasmacytoma and rat lymphocytes immunized against mouse M phi. It bound more avidly to activated than to resident M phi. ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170305
更新日期:1987-03-01 00:00:00
abstract::Antigen stimulation induces specific CD4 T cells to change from a resting phenotype (CD45RC+) to a "memory" phenotype (CD45RC-), an isoform switch that is reversible and regulated by persisting antigen. We show here that CD4 T cells responsible for mediating allograft rejection undergo a similar CD45RC+ to CD45RC- swi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199907)29:07<2177::AID-IMM
更新日期:1999-07-01 00:00:00
abstract::Because of their plasticity and central role in orchestrating immunity and tolerance, DCs can respond to pregnancy-specific signals, thus promoting the appropriate immune response in order to support pregnancy. Here, we show that pregnancy-specific glycoprotein (PSG1a), the major variant of PSG released into the circu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142140
更新日期:2012-06-01 00:00:00
abstract::Activation of T cells was shown to up-regulate the Fas ligand (FasL) which binds to the CD95 (APO-1/Fas) antigen and mediates activation-induced cell death (AICD) of activated T cells and T lymphoma cells. A recent report showed that mouse B cells express the FasL upon activation with lipopolysaccharide (LPS). We ther...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270433
更新日期:1997-04-01 00:00:00
abstract::Transcription factors (TFs) regulate cell-type-specific gene expression programs by combinatorial binding to cis-genomic elements, particularly enhancers, subsequently leading to the recruitment of cofactors, and the general transcriptional machinery to target genes. Using data integration of genome-wide TF binding pr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545713
更新日期:2015-11-01 00:00:00
abstract::An important aspect of clinical immunity to malaria is the ability to down-regulate inflammatory responses, once parasitaemia is under control, in order to avoid immune-mediated pathology. The role of classical (CD4(+)CD25(+)CD127(lo/-)FOXP3(+)) Treg in this process, however, remains controversial. Thus, we have chara...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200839112
更新日期:2009-05-01 00:00:00
abstract::Unexpected fetal loss is one of the common complications of pregnancy; however, the pathogenesis of many miscarriages, particularly those not associated with infections, is unknown. We previously found that activated DEC-205+ dendritic cells (DCs) and NK1.1+ invariant natural killer T (iNKT) cells are recruited into t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747162
更新日期:2018-06-01 00:00:00
abstract::Monoclonal gammopathies (MG) are monoclonal proliferative disorders of B cells at the differentiation stage of Ig production. They can be detected in the serum, either as transient or as persistent homogenous immunoglobulin (H-Ig) components. The exact phenotype, localization, and cell lineage origin of the precursor ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270943
更新日期:1997-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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abstract::PA28alpha/beta is a regulatory complex of the 20S proteasome which consists of two IFN-gamma inducible subunits. Both subunits, alpha and beta, contribute equally to the formation of hexa- or heptameric rings which can associate with the 20S proteasome. Previously, we have shown that overexpression of the PA28alpha su...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200012)30:12<3672::AID-IMMU3672>
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230839
更新日期:1993-08-01 00:00:00
abstract::Mercuric chloride (HgCl2) has contrasting effects on different rat strains: susceptible strains, e.g. Brown Norway (BN) develop polyclonal B cell activation, multiple autoantibodies and widespread tissue injury. Lewis (LEW) rats are resistant: no autoimmune response occurs after HgCl2; instead, there is immunosuppress...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261018
更新日期:1996-10-01 00:00:00
abstract::Although most cells of the T cell receptor (TcR) gamma/delta lineage are CD4-CD8-, some gamma/delta cells express CD8. We show here that mouse thymic gamma/delta cells express CD8 following in vitro activation by concanavalin A. Staining with monoclonal and polyclonal antibodies to the CD8 alpha (Ly-2) and CD8 beta (L...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200431
更新日期:1990-04-01 00:00:00
abstract::Physical exercise, mental stress, or infusion of beta-adrenergic agonists result in an increase in the number of natural killer (NK) cells in the peripheral circulation. In view of the specific migration pattern of NK cells in vivo, it has been suggested that these cells may be released from the marginating pool in bl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231230
更新日期:1993-12-01 00:00:00
abstract::Induction of cytolytic activity of peripheral blood mononuclear cells towards target cells was studied by preparing bispecific F(ab')2 which was composed of two Fab' fragments, one of which was derived from anti-CD3 monoclonal antibody and the other from anti-tumor monoclonal antibody. After reduction of the interchan...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190814
更新日期:1989-08-01 00:00:00
abstract::The generation of effective type 1 T helper (Th1)-cell responses is required for immunity against intracellular bacteria. However, some intracellular bacteria require interleukin (IL)-17 to drive Th1-cell immunity and subsequent protective host immunity. Here, in a model of Mycobacterium bovis Bacille Calmette-Guerin ...
journal_title:European journal of immunology
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doi:10.1002/eji.201141569
更新日期:2012-02-01 00:00:00
abstract::Pre-graft priming of heart allograft recipients with donor strain blood induces tolerance in 100% of adult rats in the congenic LEW.1W to LEW.1A combination. This tolerant state is specific for donor MHC antigens as third-party blood transfusions fail to induce tolerance, and third-party skin grafts are promptly rejec...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200105)31:5<1333::AID-IMMU1333>3
更新日期:2001-05-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323431
更新日期:2003-03-01 00:00:00
abstract::The inflammatory process that underlies allergic diseases such as asthma is characterized by tissue infiltration of eosinophils and T cells. We have used the Stamper-Woodruff frozen-section assay to characterize the receptors involved in adhesion of human peripheral blood T cells to nasal polyp endothelium (NPE) as a ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199904)29:04<1324::AID-IMM
更新日期:1999-04-01 00:00:00
abstract::Chlamydia pneumoniae stimulates potently maturation of and cytokine secretion by bone marrow-derived dendritic cells (BMDDC). BMDDC responses depend mainly on Toll-like receptor (TLR)2 and to a minor extent on TLR4. We demonstrate here using C. pneumoniae in an infectious model with the replication-permissive epitheli...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2002-09-01 00:00:00