Abstract:
:There are no direct means to study class I MHC presentation in human normal or diseased cells. Using CMV-infected human cells and applying novel mAb that mimic T-cell receptor specificity directed toward the immunogenic epitope of the viral pp65 protein presented on HLA-A2 molecules, we directly imaged the dynamics of Ag presentation in infected cells. We demonstrate that following infection large intracellular pools of HLA-A2/pp65 complexes are localized to the Golgi. These HLA-A2/pp65 pools account for the majority of total HLA-A2 molecules in infected cells. Interestingly, these large pools are sequestered inside infected cells and only a small portion of them are exported to the cell surface. Virus-induced class I MHC down-regulation did not affect the intracellular pool of HLA-A2/pp65 complexes. Our data also suggest that proteasome function influences the release of class I complexes to the membrane. We present herein a new and direct molecular tool to study the dynamics of viral Ag presentation that may further elucidate the balance between immune response versus viral escape.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Makler O,Oved K,Netzer N,Wolf D,Reiter Ydoi
10.1002/eji.200939875subject
Has Abstractpub_date
2010-06-01 00:00:00pages
1552-65issue
6eissn
0014-2980issn
1521-4141journal_volume
40pub_type
杂志文章abstract::LIGHT is a recently identified member of the TNF superfamily that is up-regulated on activated T cells and can cooperate with CD40L to condition DC for the priming of CTL. In this report, we show that an intracellular pool of LIGHT is found selectively in some CD45RA-CD27+CD4+ "central" memory T cells and to a greater...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324410
更新日期:2003-11-01 00:00:00
abstract::An abnormal protein, from a petient with mu-chain disease has been studied: protein BUR. It is devoid of the F (ab'')2 mu fragment; molecular weight determinations and immunological data identify the protein as a F(c) 5 mu fragment. Similarly, carbohydrate determinations and proteolysis experiments relate it to a Fcmu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050306
更新日期:1975-03-01 00:00:00
abstract::A comparison of the clonal nature of the immune response to phosphorylcholine (PC) was made in nine different inbred mouse strains. Quantitative idiotypic analysis showed that anti-PC antibodies from each strain were composed of antibodies bearing binding-site idiotypic determinants indistinguishable from two differen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830061002
更新日期:1976-10-01 00:00:00
abstract::Subcutaneous immunization with hen egg-white lysozyme (HEL) in complete Freund's adjuvant induces, both in antibody responder and nonresponder mice, a classical delayed-type hypersensitivity (DTH) reaction evaluated as footpad swelling. This response can be specifically transferred to naive recipients by Lyt-1+2- T ce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140910
更新日期:1984-09-01 00:00:00
abstract::Regulatory T (Treg) cells are a constitutively immunosuppressive subtype of T cells that contribute to the maintenance of immunological self-tolerance and immune homeostasis. However, the molecular mechanisms involved in the regulation of Treg cells remain unclear. In the present study, we identified ubiquitously expr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343394
更新日期:2014-02-01 00:00:00
abstract::IL-6-specific autoantibodies (aAb-IL-6) have been reported in diseased and healthy individuals. We recently established a model for aAb-IL-6 in different mouse strains, based on vaccination with immunogenic IL-6 analogues, in which titers of aAb-IL-6 above 1,000 resulted in an in vivo IL-6 deficiency. Here, we examine...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425268
更新日期:2004-11-01 00:00:00
abstract::Dendritic cells (DC) play an essential role in the induction of primary immune responses; however, very little information is available on cytokine production by DC. Here we determined the cytokine gene expression profile of two immortalized DC clones, CB1 and D2SC/1, both generated from mouse spleen but differing in ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830241039
更新日期:1994-10-01 00:00:00
abstract::We have previously reported that a subset of T cells in T cell receptor (TCR)-transgenic mice may express two different alpha chains on their surface. The expression of two functional alpha chains has also been demonstrated for human peripheral blood T cells. In this report, we show that a proportion of normal murine ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250622
更新日期:1995-06-01 00:00:00
abstract::The human promyelocytic U937 cells express detectable levels of MHC class II (MHC-II) molecules. Treatment with 12-o--tetradecanoyl phorbol 13-acetate (TPA), inducing macrophage-like differentiation, produces a dramatic decrease of MHC-II expression as result of down-modulation of the activation of immune response gen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425378
更新日期:2005-02-01 00:00:00
abstract::The pool of hematopoietic stem cells (HSCs) in the bone marrow is a mixture of resting, proliferating, and differentiating cells. Long-term repopulating HSCs (LT-HSC) are routinely enriched as Lin- Sca1+ c-Kit+ CD34- Flt3- CD150+ CD48- cells. The Flt3 ligand (Flt3L) and its receptor Flt3 are important regulators of HS...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201646730
更新日期:2017-09-01 00:00:00
abstract::The viral CC chemokine macrophage inhibitory protein-II (vMIP-II) encoded by human herpes virus 8 (HHV-8) binds to multiple chemokine receptors, however, its ability to control the initial recruitment of specific leukocyte subtypes from the peripheral circulation has not been fully clarified. Here we show that vMIP-II...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200108)31:8<2458::aid-immu24
更新日期:2001-08-01 00:00:00
abstract::This Viewpoint series provides authoritative and detailed outlines of exciting areas of DC research. Some of the subjects that frequently come up include development of DC; distribution of DC in lymphoid and non-lymphoid tissues such as skin, intestine and lung; different forms or subsets of DC; and the role of DC in ...
journal_title:European journal of immunology
pub_type:
doi:10.1002/eji.201040733
更新日期:2010-08-01 00:00:00
abstract::The monoclonal antibody W6/32.1 recognizes a public determinant on the HLA-A, B and C antigens of all tested human haplotypes. Though the antibody does not bind to normal mouse cells of any H-2 haplotype, it does show an unexpected specificity for the T cell leukemia line MBL-2 from a C57BL/6 mouse. It is shown that t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130213
更新日期:1983-02-01 00:00:00
abstract::The deposition of immune complexes, followed by activation of complement and/or Fc receptors and generation of chemoattractants, is the most common feature of human glomerulonephritis. Recently we have shown that primary cultured human glomerular mesangial cells (HMC), which are normally negative for IgG Fc receptors,...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199809)28:09<2928::AID-IMM
更新日期:1998-09-01 00:00:00
abstract::Human T cell clones expressing the T cell receptor (TcR) gamma/delta were isolated from peripheral blood lymphocytes of two unrelated donors. The TcR gamma/delta+ clones derived from one of these donors were all of the Ti gamma A+, delta-TCS1-, BB3+ phenotype indicating the exclusive use of the V gamma 9 and V delta 3...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191120
更新日期:1989-11-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230530
更新日期:1993-05-01 00:00:00
abstract::A monoclonal antibody (mAb), 2D1(IgM), was identified for its anti-proliferative effect on human T leukemia cell line, SUP-T13. The cells bound with 2D1 showed DNA ladder patterns of oligonucleosomes, demonstrating apoptosis. Peripheral mononuclear cells activated by phytohemagglutinin or OKT3 induced expression of 2D...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230831
更新日期:1993-08-01 00:00:00
abstract::Antibodies to framework determinants of the VH and V lambda fragments of MOPC 315 and antisera to the VH idiotype determinants of the A 5 A antibody were used to analyze the antigen receptors of mouse T (and B) cells. This was done by using the antibodies as inhibitors in (a) an assay in which the binding of radiolabe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830100207
更新日期:1980-02-01 00:00:00
abstract::A monoclonal antibody (mAb) directed to a human leukocyte 55-kDa cell surface molecule with identical cellular distribution and biochemical properties to the CD4 was able to inhibit T cell proliferation induced either in a mixed lymphocyte culture or by activation with mAb anti-CD3, anti-CD2 or phytohemagglutinin. The...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170205
更新日期:1987-02-01 00:00:00
abstract::Expression of activation markers and proliferative status were measured in peripheral CD4+ and CD8+ T cells of various T cell receptor (TCR)-transgenic mice either before or after intentional antigenic stimulation. In the absence of intentional immunization, CD4+ T cells persisted as resting or partially activated and...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261251
更新日期:1996-12-01 00:00:00
abstract::In mouse Peyer's patches (PP), dendritic cells (DC) are localized in T cell areas as NLDC145+ CD11c+ cells, and in the dome and corona region of the follicle as NLDC145- CD11c+ cells, respectively, suggesting the presence of two different DC populations with distinct roles in antigen uptake, processing, and presentati...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270605
更新日期:1997-06-01 00:00:00
abstract::Naive and central memory CD8 T cells use CCR7 to recirculate through T cell zones of secondary lymphoid organs where they can encounter antigen. Here we describe a subset of human CD8 T cells expressing CXCR5 which enables homing in response to CXCL13 produced within B cell follicles. CXCR5+ CD8 T cells were found in ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636746
更新日期:2007-12-01 00:00:00
abstract::Deleted in malignant brain tumors 1 (DMBT1) is a secreted glycoprotein displaying a broad bacterial-binding spectrum. Recent functional and genetic studies linked DMBT1 to the suppression of LPS-induced TLR4-mediated NF-kappaB activation and to the pathogenesis of Crohn's disease. Here, we aimed at unraveling the mole...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838689
更新日期:2009-03-01 00:00:00
abstract::The present experiments address functional antibody diversity and clonal distribution in murine available repertoires. IgM-containing supernatants were prepared by unbiased, polyclonal stimulation of resting splenic B cells from C57BL/6 mice, to ensure similar numbers of responding clones/culture and equivalent growth...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199804)28:04<1204::AID-IMM
更新日期:1998-04-01 00:00:00
abstract::Evidence is presented that the phenotype of the rat T helper cells is stable after priming and boosting with a hapten-carrier conjugate and that the phenotype is W3/25+, MRC OX-22-, MRC OX-8-. Data are also presented that demonstrate the presence of doubly marked, W3/25+, MRC OX-8+, cells in the spleens of rats primed...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130916
更新日期:1983-09-01 00:00:00
abstract::The magnitude and quality of T cell responses generated when Ag is targeted to receptors on DC is influenced by both the specific receptor targeted and its distribution among DC subsets. Here we examine the targeting of the model Ag OVA to potential DC targets, including CD11c, CD205, MHC class II, CD40, TLR2 and Fcga...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838302
更新日期:2008-08-01 00:00:00
abstract::Two cloned murine helper T lymphocyte (HTL) lines were used to investigate the immunoregulatory properties of highly purified interleukin 2 (IL2). The clone, designated J6.19, secretes lymphokines, including IL2, and proliferates when stimulated with ovalbumin in the presence of I-Ak-bearing spleen cells, while the al...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160302
更新日期:1986-03-01 00:00:00
abstract::Patients undergoing allogeneic bone marrow transplantation offer a unique system to analyze NK cell development in vivo. We analyzed NK cells from 23 such patients to assess the acquisition of activating receptors. Four patients displayed an immature NK cell surface phenotype at engraftment, as their cells were CD16(-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324668
更新日期:2004-02-01 00:00:00
abstract::The TNF member LIGHT also known as TL4 or TNFSF14) can play a major role in cancer control via its two receptors; it induces tumor cell death through lymphotoxin-beta receptor (LT-betaR) and ligation to the herpes virus entry mediator (HVEM) amplifies the immune response. By studying the effect of LIGHT in the transcr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939069
更新日期:2009-09-01 00:00:00
abstract::In order to analyze the effect of a high local concentration of macrophage colony-stimulating factor (M-CSF; CSF-1) on tumor growth, the plasmacytoma cell line J558L was transfected with the human M-CSF gene and injected into syngeneic BALB/c mice. In contrast to the parental tumors, M-CSF transfectants were heavily i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230129
更新日期:1993-01-01 00:00:00