Interleukin-5 mRNA expressed by eosinophils and gamma/delta T cells in parasite-immune sheep.

Abstract:

:Interleukin (IL)-5 is produced by variety of cell types and contributes to both lymphocyte development an eosinophil terminal differentiation in vitro. The coincidence of worm expulsion and eosinophilia in sheep infected with the gastrointestinal nematode Trichostrongylus colubriformis suggest that eosinophils may be involved as effector cells in host immunity against parasite infection. The role of IL-5 in this process was investigated by observing the distribution of IL-5 mRNA+ cells in the small intestine, mesenteric lymph nodes (MLN) and Peyer's patches (PP) by an in situ hybridization technique using a murine IL-5 riboprobe. IL-5 mRNA+ cells were distributed throughout the lamina propria (LP) of the small intestine from the tips of the villi to the muscularis mucosae and in the parafollicular areas of MLN and PP in both naive and immune sheep. The phenotypes of IL-5 mRNA+ cells was explored by simultaneous eosin and immunohistochemical staining using a monoclonal antibody recognizing the T19 marker, which identifies a major subset of gamma/delta TCR+ cells in sheep. In immune sheep, there was about a five-fold increase in the number of eosinophils and IL-5 cell mRNA+ cells in the LP, but there was no significant change in numbers of T19+ cells. Most of the IL-5 mRNA cells in the LP were eosinophils, but many of the T19+ cells also expressed IL-5 mRNA. In contrast, there were fewer eosinophils than T19+ cells in MLN of immune sheep and, compared to controls, a three-fold increase in T19+ cells and a five-fold increase in T19+/IL-5 mRNA+ double positive cells observed in immune sheep. In PP, there were very few eosinophils but substantial numbers of T19+ cells; however, no significant differences in numbers of eosinophils, T19+ or IL-5 mRNA+ cells were observed between control and immune sheep. These results indicate that in sheep, both eosinophils and gamma/delta T cells are capable of IL-5 expression and suggest that IL-5 is an important regulatory factor in autocrine and paracrine activation of effector cells involved in parasite immune expulsion.

journal_name

Eur J Immunol

authors

Bao S,McClure SJ,Emery DL,Husband AJ

doi

10.1002/eji.1830260308

subject

Has Abstract

pub_date

1996-03-01 00:00:00

pages

552-6

issue

3

eissn

0014-2980

issn

1521-4141

journal_volume

26

pub_type

杂志文章
  • Lethal Escherichia coli and Salmonella typhimurium endotoxemia is mediated through different pathways.

    abstract::Despite the differences in the molecular structure between lipopolysaccharides (LPS) isolated from Escherichia coli, Klebsiella pneumoniae or Salmonella typhimurium, the potential differences in their biological effects in vivo have not been investigated. In the present study, TNF and LT double knock-out (TNF-/-LT-/-)...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/1521-4141(200109)31:9<2529::aid-immu25

    authors: Netea MG,Kullberg BJ,Joosten LA,Sprong T,Verschueren I,Boerman OC,Amiot F,van den Berg WB,Van der Meer JW

    更新日期:2001-09-01 00:00:00

  • Omalizumab dampens type 2 inflammation in a group of long-term treated asthma patients and detaches IgE from FcεRI.

    abstract::Even if omalizumab is broadly used in the treatment of severe, allergic asthma, the immunological effects in long-term treated patients have not been fully elucidated. To this aim, a cohort of 15 allergic asthmatic patients treated with omalizumab for at least three years was compared with 12 allergic asthma patients ...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201847668

    authors: Maggi L,Rossettini B,Montaini G,Matucci A,Vultaggio A,Mazzoni A,Palterer B,Parronchi P,Maggi E,Liotta F,Annunziato F,Cosmi L

    更新日期:2018-12-01 00:00:00

  • Differential role of IL-18 and IL-12 in the host defense against disseminated Candida albicans infection.

    abstract::IFN-gamma plays a crucial role in the defense against infection with Candida albicans. Since IL-18 and IL-12 are strong stimuli of IFN-gamma production, we investigated whether endogenous IL-18 and IL-12 are involved in the host defense during disseminated candidiasis. IL-18 knockout (IL-18-/-) mice, but not IL-12-/- ...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200323737

    authors: Netea MG,Vonk AG,van den Hoven M,Verschueren I,Joosten LA,van Krieken JH,van den Berg WB,Van der Meer JW,Kullberg BJ

    更新日期:2003-12-01 00:00:00

  • Group 1 CD1-restricted T cells take center stage.

    abstract::The recognition of lipid antigens by T cells is a complex and fascinating phenomenon. The MHC-like molecules of the CD1 family have evolved to present a wide variety of both self and foreign lipids for recognition by T-cell receptors. While much progress has been made in our understanding of the NKT cells that recogni...

    journal_title:European journal of immunology

    pub_type: 评论,杂志文章

    doi:10.1002/eji.201141408

    authors: Young MH,Gapin L

    更新日期:2011-03-01 00:00:00

  • Demonstration of T lymphocytotoxic human fetal antibody against maternal T and concanavalin A-inducible adult T cells in cord IgM.

    abstract::To clarify reasons of the constant presence of IgM in human cord blood, some functions of this immunoglobulin class were analyzed. Cord IgM was collected from cord blood, and the cord IgM F(ab')2 fragment was obtained by trypsin cleavage. Maternal T and adult T cells were stained with fluorescein isothiocyanate (FITC)...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830110208

    authors: Miyagawa Y,Komiyama A,Akabane T

    更新日期:1981-02-01 00:00:00

  • Cyclic tetramolecular complexes of monoclonal antibodies: a new type of cross-linking reagent.

    abstract::A simple and efficient procedure for the construction of bifunctional molecules is described and their use in a variety of applications documented. This procedure is based on our observation that mouse IgG1 monoclonal antibodies, when mixed with equimolar amounts of a high-affinity rat monoclonal antibody specific for...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830160615

    authors: Lansdorp PM,Aalberse RC,Bos R,Schutter WG,Van Bruggen EF

    更新日期:1986-06-01 00:00:00

  • Inverse relationship of CA2+ mobilization and cell proliferation in CD8+ memory and virgin T cells.

    abstract::Memory T cells can now be defined with various monoclonal antibodies but little is known about the functional properties of these cells as compared to virgin T cells. We have studied Ca2+ mobilization and proliferation of memory and virgin cells in both the CD4 and CD8 subsets in response to phytohemagglutin-in. Using...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830200913

    authors: Schlunck T,Schraut W,Riethmüller G,Ziegler-Heitbrock HW

    更新日期:1990-09-01 00:00:00

  • IL-10 interrupts memory B cell expansion in the germinal center by inducing differentiation into plasma cells.

    abstract::Germinal center (GC) B cells undergo proliferation, somatic hypermutation and isotype switching in the course of differentiation into plasma cells to produce high-affinity antibodies. To understand the molecular mechanism regulating the expansion of memory B cells and the termination of expansion by differentiation in...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1521-4141(199802)28:02<508::AID-IMMU

    authors: Choe J,Choi YS

    更新日期:1998-02-01 00:00:00

  • Mycobacterium tuberculosis exploits the CD95/CD95 ligand system of gammadelta T cells to cause apoptosis.

    abstract::Vgamma9/Vdelta2+ T cells specifically recognize Mycobacterium tuberculosis in vitro and are precociously recruited in early mycobacterial lesions. Even if gammadelta T cells are only fortuitously detected in granulomas or bronchoalveolar lavages of patients with active pulmonary tuberculosis, a role in shaping the mat...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1521-4141(199806)28:06<1798::AID-IMM

    authors: Manfredi AA,Heltai S,Rovere P,Sciorati C,Paolucci C,Galati G,Rugarli C,Vaiani R,Clementi E,Ferrarini M

    更新日期:1998-06-01 00:00:00

  • Double-positive T cell receptor(high) thymocytes are resistant to peptide/major histocompatibility complex ligand-induced negative selection.

    abstract::To investigate negative selection events during intrathymic ontogeny, we established T cell receptor (TCR)-transgenic mice [N15tg/RAG-2-/- (H-2b)] expressing a single TCR specific for vesicular stomatitis virus nuclear octapeptide N52-59 (VSV8) in the context of the major histocompatibility complex (MHC) class I molec...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830270923

    authors: Ghendler Y,Hussey RE,Witte T,Mizoguchi E,Clayton LK,Bhan AK,Koyasu S,Chang HC,Reinherz EL

    更新日期:1997-09-01 00:00:00

  • Genetic and functional characterization of an antiserum to the lipid A-specific triggering receptor on murine B lymphocytes.

    abstract::The inheritance of responsiveness to lipopolysaccharide (LPS), and of a marker recognized in LPS-reactive cells by a heterologous antiserum, was studied in crosses between C3H/HeJ (nonresponder) and C3H/Tif (high responder) mice. F1 hybrid mice show codominant expression of these traits: (a) LPS-reactive cells are onl...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830080113

    authors: Coutinho A,Forni L,Watanabe T

    更新日期:1978-01-01 00:00:00

  • Oxpentifylline inhibits tumor necrosis factor-alpha mRNA transcription and protects against arthritis in mercuric chloride-treated brown Norway rats.

    abstract::The phosphodiesterase inhibitor oxpentifylline (OXP) has a number of potentially important immunomodulatory actions which include a selective inhibition of the Th1 subset of CD4+ cells in vitro and inhibition of tumor necrosis factor (TNF)-alpha mRNA transcription. In vivo, it has a dramatic protective effect against ...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830251029

    authors: Kiely PD,Gillespie KM,Oliveira DB

    更新日期:1995-10-01 00:00:00

  • Activation of human T lymphocytes. III. Triggering of bystander cytotoxicity in cytotoxic T cell clones by antibodies against the T3 antigen or by a calcium ionophore.

    abstract::The role of T cell differentiation antigens in antigen-specific and nonspecific cytotoxicity by human cytotoxic T lymphocyte (CTL) clones was investigated. In contrast to other reports, several monoclonal antibodies (mAb) against the T3 antigen only marginally blocked antigen-specific cytotoxicity at high concentratio...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830151011

    authors: Schrezenmeier H,Kurrle R,Wagner H,Fleischer B

    更新日期:1985-10-01 00:00:00

  • MMCP-8, the first lineage-specific differentiation marker for mouse basophils. Elevated numbers of potent IL-4-producing and MMCP-8-positive cells in spleens of malaria-infected mice.

    abstract::In mice infected with the non-lethal malaria parasite Plasmodium chabaudi chabaudi AS, a prominent switch from a Th1 to a Th2 type of response occurs in CD4+ T cells at the time of peak parasitemia or shortly thereafter (9-15 days after infection). This is accompanied by a major increase in IL-4, and a similar decreas...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/1521-4141(200009)30:9<2660::AID-IMMU2660>3

    authors: Poorafshar M,Helmby H,Troye-Blomberg M,Hellman L

    更新日期:2000-09-01 00:00:00

  • Activation and activation-induced death of human tonsillar B cells and Burkitt lymphoma cells: lack of CD95 (Fas/APO-1) ligand expression and function.

    abstract::Activation of T cells was shown to up-regulate the Fas ligand (FasL) which binds to the CD95 (APO-1/Fas) antigen and mediates activation-induced cell death (AICD) of activated T cells and T lymphoma cells. A recent report showed that mouse B cells express the FasL upon activation with lipopolysaccharide (LPS). We ther...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830270433

    authors: Daniel PT,Oettinger U,Mapara MY,Bommert K,Bargou R,Dörken B

    更新日期:1997-04-01 00:00:00

  • Transgene-mediated hyper-expression of IL-5 inhibits autoimmune disease but increases the risk of B cell chronic lymphocytic leukemia in a model of murine lupus.

    abstract::IL-5 preferentially activates B1 cells to produce natural antibodies cross-reactive to self antigens. To determine the role of IL-5 in antibody-mediated autoimmune disease, we generated systemic lupus erythematosus (SLE)-prone (NZB x NZW)F1 mice congenic for IL-5 transgene (TG-F1). The transgene unexpectedly reduced t...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200425267

    authors: Wen X,Zhang D,Kikuchi Y,Jiang Y,Nakamura K,Xiu Y,Tsurui H,Takahashi K,Abe M,Ohtsuji M,Nishimura H,Takatsu K,Shirai T,Hirose S

    更新日期:2004-10-01 00:00:00

  • Regulation of CD69 expression on human natural killer cells: differential involvement of protein kinase C and protein tyrosine kinases.

    abstract::Human peripheral blood natural killer (NK) cells (CD56+, CD16+, CD3 epsilon- lymphocytes) express CD69 after their stimulation by interleukin-2 (IL-2) or interferon-alpha (IFN-alpha). This activation antigen represents a triggering surface molecule in NK cell clones as its stimulation triggers the cytolytic machinery ...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830230509

    authors: Borrego F,Peña J,Solana R

    更新日期:1993-05-01 00:00:00

  • Characterization of the NPa idiotype through the analysis of monoclonal BALB/c anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies.

    abstract::Spleen cells from BALB/c mice were fused with the nonsecreting myeloma line X63.Ag8.6.5.3 seven days after immunization with NP-CG (4-hydroxy-3-nitrophenyl) acetyl-chicken gamma-globulin. The hybrid cell lines obtained were analyzed for heavy and light chain distribution, fine specificity, and idiotype. The majority o...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830111109

    authors: White-Scharf ME,Imanishi-Kari T

    更新日期:1981-11-01 00:00:00

  • Dynamic changes in chromatin conformation at the TNF transcription start site in T helper lymphocyte subsets.

    abstract::Tumor necrosis factor (TNF) is one of the key primary response genes in the immune system that can be activated by a variety of stimuli. Previous analysis of chromatin accessibility to DNaseI demonstrated open chromatin conformation of the TNF proximal promoter in T cells. Here, using chromatin probing with restrictio...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201243297

    authors: Shebzukhov YV,Horn K,Brazhnik KI,Drutskaya MS,Kuchmiy AA,Kuprash DV,Nedospasov SA

    更新日期:2014-01-01 00:00:00

  • Isolation of the intact white pulp. Quantitative and qualitative analysis of the cellular composition of the splenic compartments.

    abstract::The spleen is anatomically and functionally divided into two compartments: the red pulp, where particles are effectively removed from the blood, and the white pulp, where specific immune responses are generated. Here the isolation of white pulp from red pulp is described, allowing a detailed analysis of the cellular c...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/1521-4141(200002)30:2<626::AID-IMMU626>3.0

    authors: Nolte MA,Hoen EN,van Stijn A,Kraal G,Mebius RE

    更新日期:2000-02-01 00:00:00

  • Direct helper T cell-induced B cell differentiation involves interaction between T cell antigen CD28 and B cell activation antigen B7.

    abstract::Cognate interactions between major histocompatibility complex class II antigen (Ag)-reactive CD4+ T helper (Th) and Ag-presenting B cells induce first the activation of B cells and their subsequent differentiation into Ig-secreting cells (IgSC). The Th cell-associated homodimeric glycoprotein CD28 has been implicated ...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830210527

    authors: Damle NK,Linsley PS,Ledbetter JA

    更新日期:1991-05-01 00:00:00

  • Fasciola hepatica tegumental antigens induce anergic-like T cells via dendritic cells in a mannose receptor-dependent manner.

    abstract::FoxP3(+) Treg cells and anergic T cells are the two regulatory phenotypes of T-cell responses associated with helminth infection. Here, we examine the T-cell responses in mice during Fasciola hepatica infection, and to its tegumental coat antigens (FhTeg) that are shed from the fluke every 2-3 h. FhTeg comprises a ric...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201545905

    authors: Aldridge A,O'Neill SM

    更新日期:2016-05-01 00:00:00

  • Blockade of PD-1 or p38 MAP kinase signaling enhances senescent human CD8(+) T-cell proliferation by distinct pathways.

    abstract::Immune enhancement is desirable in situations where decreased immunity results in increased morbidity. We investigated whether blocking the surface inhibitory receptor PD-1 and/or p38 MAP kinase could enhance the proliferation of the effector memory CD8(+) T-cell subset that re-expresses CD45RA (EMRA) and exhibits cha...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201445312

    authors: Henson SM,Macaulay R,Riddell NE,Nunn CJ,Akbar AN

    更新日期:2015-05-01 00:00:00

  • NKG2D regulates production of soluble TRAIL by ex vivo expanded human γδ T cells.

    abstract::Soluble TRAIL (sTRAIL) can be produced by myeloid-derived cells to kill cancer cells. Whether this mechanism is used by T cells, and if so, how sTRAIL production is regulated, remains unclear. Our previous studies showed that ex vivo expanded human γδ T cells express TRAIL and NK receptor group 2 (R2), member D (NKG2D...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201243150

    authors: Dokouhaki P,Schuh NW,Joe B,Allen CA,Der SD,Tsao MS,Zhang L

    更新日期:2013-12-01 00:00:00

  • Mincle, the receptor for mycobacterial cord factor, forms a functional receptor complex with MCL and FcεRI-γ.

    abstract::Upon receptor activation, the myeloid C-type lectin receptor Mincle signals via the Syk-CARD9-Bcl10-MALT1 pathway. It does so by recruiting the ITAM-bearing FcεRI-γ. The related receptor macrophage C-type Lectin (MCL) has also been shown to be associated with Syk and to be dependent upon this signaling axis. We have p...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201343752

    authors: Lobato-Pascual A,Saether PC,Fossum S,Dissen E,Daws MR

    更新日期:2013-12-01 00:00:00

  • Four types of Ca2+ signals in naive CD8+ cytotoxic T cells after stimulation with T cell agonists, partial agonists and antagonists.

    abstract::Stimulation of T cells via the T cell receptor (TCR) leads to an increase intracellular in free Ca2+ levels ([Ca2+]i) and the activation of the MAP kinase signaling pathway. This study analyzes for the first time Ca2+ fluxes in naive cytotoxic T cells stimulated with full agonists, partial agonists, or antagonists. Fo...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830271241

    authors: Bachmann MF,Mariathasan S,Bouchard D,Speiser DE,Ohashi PS

    更新日期:1997-12-01 00:00:00

  • Cell death and thymic export during fetal life.

    abstract::In the fetus the peripheral T cell pool expands as the fetus grows, but the mechanisms that regulate T cell homeostasis during fetal life are unknown. Here, we show that the peripheral T cell pool in the sheep fetus is established by the export from the fetal thymus of twice as many CD8+ as CD4+ thymic emigrants every...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200636096

    authors: Holder JE,Washington EA,Cunningham CP,Cahill RN,Kimpton WG

    更新日期:2006-10-01 00:00:00

  • CD11c provides an effective immunotarget for the generation of both CD4 and CD8 T cell responses.

    abstract::The magnitude and quality of T cell responses generated when Ag is targeted to receptors on DC is influenced by both the specific receptor targeted and its distribution among DC subsets. Here we examine the targeting of the model Ag OVA to potential DC targets, including CD11c, CD205, MHC class II, CD40, TLR2 and Fcga...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200838302

    authors: Castro FV,Tutt AL,White AL,Teeling JL,James S,French RR,Glennie MJ

    更新日期:2008-08-01 00:00:00

  • Interleukin-18 induces production of proinflammatory cytokines in mice: no intermediate role for the cytokines of the tumor necrosis factor family and interleukin-1beta.

    abstract::Interleukin-18 (IL-18) is not only a co-stimulus for the induction of interferon-gamma but also has direct proinflammatory effects by inducing tumor necrosis factor-alpha (TNF-alpha), IL-1, IL-8 and IL-6. However, the cascade of events leading to induction of cytokines by IL-18 is unclear. The aim of the present study...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/1521-4141(200010)30:10<3057::AID-IMMU3057>

    authors: Netea MG,Kullberg BJ,Verschueren I,Van Der Meer JW

    更新日期:2000-10-01 00:00:00

  • The selective localization of B lymphocytes in the spleen and the role of complement receptors.

    abstract::The role of complement receptors on the localization of T and B cells in the spleen of mice was studied using short-term homing experiments in cobra venom factor (CoF)-treated animals. The localization ratio of B and T cells in the spleen of CoF-treated mice decreased significantly compared to control recipients. No c...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830150708

    authors: Kraal G,Van Hoogstraten I,Klerx JP,Van Dijk H

    更新日期:1985-07-01 00:00:00