Abstract:
:T cell receptor (TCR) genes are initially activated in hematopoietic stem cells that have been committed to the T cell lineage during ontogeny. We have established cell lines derived from hematopoietic organs which express truncated TCR delta mRNA (1.3 kbp). In this study, cDNA clones of this mRNA were analyzed to characterize TCR delta gene activation in early T cell development. Transcription started from an intron between D delta 2 and J delta 1, 72 bp upstream from J delta 1, and continued to J delta 1 and C delta exons, which were directly spliced to J delta 1. Thus, the truncated mRNA expressed by the cell lines was determined to be a germ-line transcript. The promoter activity of the DNA fragment between D delta 2 and J delta was assessed by its ability to drive the transcription of a reporter gene linked to it in the cell line expressing the TCR delta germ-line transcript. TCR delta germ-line transcription was found not only in these cell lines but also in fetal liver and thymus cells. These findings indicate that the TCR delta germ-line transcription is a naturally occurring event in developing T cells. The significance of germ-line transcription of TCR delta genes is unknown, but it may be an event that follows T-lineage commitment or, at least, may be closely associated with this commitment.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Shimamura M,Ohta Sdoi
10.1002/eji.1830250611subject
Has Abstractpub_date
1995-06-01 00:00:00pages
1541-6issue
6eissn
0014-2980issn
1521-4141journal_volume
25pub_type
杂志文章abstract::Influenza virus infection is an important cause of severe asthma exacerbations, but it remains unclear how a Th1-mediated antiviral response triggers a prototypical Th2 disease. We investigated CD4+ T cells and group 2 innate lymphoid cells (ILC2s) in influenza virus-infected mice. We found that ILC2s accumulated in t...
journal_title:European journal of immunology
pub_type: 杂志文章
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journal_title:European journal of immunology
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更新日期:2003-01-01 00:00:00
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journal_title:European journal of immunology
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更新日期:2010-05-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1996-04-01 00:00:00
abstract::Autoimmune diseases including multiple sclerosis (MS) are the result of an imbalanced immune tolerance network. Dendritic cells (DCs) are key players in both initiating immunity (immunogenic DCs) and regulating immune responses (tolerogenic DCs = tolDCs) and are potential targets for the treatment of MS. While the imm...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
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更新日期:2012-03-01 00:00:00
abstract::Glomerular crescent formation characterizes severe glomerulonephritis (GN). Evidence suggests that crescent formation results from a delayed-type hypersensitivity-like Th1 response. As IL-12 directs Th1 responses, we tested the hypothesis that IL-12 is important in crescentic GN. Neutralization of IL-12 attenuated cre...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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journal_title:European journal of immunology
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更新日期:1989-06-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章,评审
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更新日期:2011-09-01 00:00:00
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journal_title:European journal of immunology
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更新日期:1989-07-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200002)30:2<356::AID-IMMU356>3.0
更新日期:2000-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180905
更新日期:1988-09-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261008
更新日期:1996-10-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250130
更新日期:1995-01-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636695
更新日期:2007-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2000012)30:12<3422::AID-IMMU3422
更新日期:2000-12-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260817
更新日期:1996-08-01 00:00:00
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更新日期:2001-04-01 00:00:00
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journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1998-08-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230411
更新日期:1993-04-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830201022
更新日期:1990-10-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150214
更新日期:1985-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2003-11-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270219
更新日期:1997-02-01 00:00:00
abstract::KARAP/DAP12 is a broadly distributed transmembrane signaling polypeptide with an immunoreceptor tyrosine-based activation motif, and is non-covalently associated with a variety of activating surface receptors. We report here the characterization of transgenic mice that overexpress KARAP/DAP12 polypeptides in both myel...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200209)32:9<2653::AID-IMMU2653>3
更新日期:2002-09-01 00:00:00
abstract::APO-1/Fas (CD95) is a transmembrane receptor that transduces apoptotic signals within various cells including T and B cells. The APO-1 gene was found to be defective in lpr mice. In these mice insertion of a retrotransposon gives rise to transcription of an abnormal mRNA and only a fraction of wild-type APO-1 mRNA. It...
journal_title:European journal of immunology
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doi:10.1002/eji.1830241231
更新日期:1994-12-01 00:00:00