Abstract:
:T cell-depleted B cells from a patient with LFA-1 deficiency were tested in costimulation assays for responsiveness to recombinant human IL4 (BSF-1) and purified low molecular weight B cell growth factor (BCGFlow). In both cases the response of LFA-1-deficient B cells was comparable with normal controls. Monoclonal antibodies to LFA-1 alpha (CD11a) and beta (CD18) chains were unable to mimic the action of IL4 on normal B cells in costimulation assays with anti-IgM, and did not inhibit normal B cell proliferation in response to IL4 and anti-IgM. Epstein-Barr virus-transformed lymphoblastoid B cell lines (LCL) from normal and LFA-1-deficient donors both responded in proliferation assays to BCGFlow but not IL4. Similarly, both normal and LFA-1-deficient LCL increased IgM secretion in response to BCDF, BCGFlow and, interestingly, IL4. The normal LCL also increased IgG secretion in response to these factors, but no IgG was detected in supernatants from the LFA-1-deficient LCL. These results show that LFA-1 expression is not essential for B cell responses to B cell growth and differentiation factors.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Shields JG,Smith SH,Strobel S,Levinsky RJ,Defrance T,De Vries J,Banchereau J,Callard REdoi
10.1002/eji.1830180212subject
Has Abstractpub_date
1988-02-01 00:00:00pages
255-9issue
2eissn
0014-2980issn
1521-4141journal_volume
18pub_type
杂志文章abstract::The glycolipid alpha-galactosylceramide (alpha-GalCer) is a potent activator of invariant natural killer T (iNKT) cells and has been shown to be an effective agent against cancer, infections and autoimmune diseases. The effectiveness of alpha-GalCer and its alkyl chain analogues depends on efficient loading and presen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737518
更新日期:2008-03-01 00:00:00
abstract::Human T cell clones (TCC) are antigen-presenting cells (APC) able to present peptides and superantigens (SAg) and to process and present intact proteins. TCC express major histocompatibility complex (MHC) class II antigens and molecules involved in the accessory signal delivery, such as B7.1 and B7.2/B70. Notwithstand...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260411
更新日期:1996-04-01 00:00:00
abstract::The host protective immune response to blood stage malaria infection was studied using Plasmodium chabaudi chabaudi (P.chabaudi) in NIH mice. It has been shown previously that CD4+ cells are critically required for protection against erythrocytic infection. Mice lacking a functional CD4+ cell compartment suffer unremi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240124
更新日期:1994-01-01 00:00:00
abstract::The specificity of the IgM receptor expressed by human T cells cultured in IgM-free media has been investigated. IgM receptors have been detected using a rosette system with ox erythrocytes coated with rabbit antibody (EA(IgM)), and the inhibitory capacity of different IgM fragments in the rosette system has been test...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060714
更新日期:1976-07-01 00:00:00
abstract::Role of CD45 in B cell antigen receptor (BcR)-mediated signaling events in mature B cells was examined using BAL-17 and its CD45-negative clones. In the CD45-negative clones, BcR stimulation induced tyrosine phosphorylation almost identical to the parental cells, with a few exceptions of reduced phosphorylation, espec...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250823
更新日期:1995-08-01 00:00:00
abstract::The cell surface molecule CD44, thought to be a cell surface receptor for hyaluronic acid, is expressed by thymocytes and peripheral T cells. Following T cell activation with mitogens or antigens, surface expression of CD44 increases. This increase in CD44 cell surface expression has been used as a means of identifyin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210632
更新日期:1991-06-01 00:00:00
abstract::Stromal elements are major components of lymphoid tissues contributing to both tissue architecture and function. In this study we report on the phenotype and function of fibroblast-like stromal cells obtained from human spleen. These cells express high levels of CD44 and ICAM-1 and moderate levels of VLA-4, VCAM, CD40...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199812)28:12<3940::AID-IMM
更新日期:1998-12-01 00:00:00
abstract::Anti-CD3 mAb can modulate graft rejection and attenuate autoimmune diseases but their mechanism(s) of action remain unclear. CD8(+) T cells with regulatory function are induced in vitro by Teplizumab, a humanized anti-CD3 antibody and inhibit responses of autologous and allogeneic T cells. They inhibit CD4(+) T-cell p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040485
更新日期:2010-10-01 00:00:00
abstract::The diversity of the human immune repertoire and how it relates to a functional immune response has not yet been studied in detail in humanized NOD.SCID.gammac(-/-) immunodeficient mice. Here, we used a multiplex PCR on genomic DNA to quantify the combinatorial diversity of all possible V-J rearrangements at the TCR-b...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939480
更新日期:2009-08-01 00:00:00
abstract::Human natural killer (NK) cells can be subdivided into two populations based on the density of cell surface CD56 antigen. The great majority (approximately 90%) of NK cells express CD56 at low levels (the CD56dim phenotype), whereas a small NK cell subset (approximately 10%) exhibits approximately fivefold greater den...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220102
更新日期:1992-01-01 00:00:00
abstract::The MOPC460 idiotype is expressed in mice with the IghCa allotypic haplotype after anti-2,4-dinitrophenyl (DNP) immunization. We have previously shown that two monoclonal syngeneic anti-idiotypic antibodies (IDM 92-13 and IDM 41-27) define two distinct idiotopes (the 460.92 and the 460.41) on the M460 idiotype. The cu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830131209
更新日期:1983-12-01 00:00:00
abstract::The induction of cytotoxic T lymphocytes (CTL) specific for alloantigen in a primary mixed lymphocyte culture (MLC) was used as a model system to study the potential usefulness of monoclonal antibodies directed against the murine transferrin receptor in eliminating antigen-activated T cells. Two monoclonal antibodies ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150709
更新日期:1985-07-01 00:00:00
abstract::Transcription of the gene encoding the transcriptional coactivator Oct-binding factor 1 (OBF-1)/OCA-B/Bob.1 is largely restricted to B cells. During B cell development OBF-1 expression shows two peaks, one in immature B cells in the bone marrow and the other in germinal center B cells. Promoter analysis has identified...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323882
更新日期:2003-10-01 00:00:00
abstract::Interferon regulatory factor-1 (IRF-1) is a transcription factor regulating the expression of several cytokines. Using IRF-1 knockout (KO) mice, the role of IRF-1 in the production and activity of IL-18 was evaluated. Administration of IL-12 or concanavalin A significantly increased levels of circulating IL-18 in wild...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200102)31:2<369::aid-immu369
更新日期:2001-02-01 00:00:00
abstract::Lymphocyte infiltration to pancreatic islets is associated to chemoattraction, as are other inflammatory autoimmune processes. We examined whether development of insulitis and diabetes depends on chemoattraction of lymphocytes via the CCR5 chemokine receptor. In non-obese diabetic (NOD) mice, a substantial fraction of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324285
更新日期:2004-02-01 00:00:00
abstract::Lipids are key regulators of immune responses. In this study we investigated the direct impact of oxidized phospholipids (ox-PL) on T cell activation and function. We could demonstrate that ox-PL strongly inhibit proliferation of purified human T cells induced with anti-CD3/CD28 or anti-CD3/CD63 mAb, whereas prolifera...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737619
更新日期:2008-03-01 00:00:00
abstract::The protooncogene HER2/neu encodes a 185-kDa transmembrane protein with extensive homology to the epidermal growth factor receptor. It is overexpressed in several human cancers of epithelial origin, such as pancreatic cancer. Previously, we demonstrated that cytotoxic T lymphocytes (CTL) derived from breast, ovarian, ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270511
更新日期:1997-05-01 00:00:00
abstract::Aging is accompanied by altered immunity, resulting in a variable state of poorly understood immunodeficiency. While both the numbers and the functionality of naïve T cells are decreased by aging, the impact of these changes upon immune defense against bacterial pathogens in vivo remains understudied. Using a model of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201041141
更新日期:2011-05-01 00:00:00
abstract::Cells expressing the chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) and the chemokine C receptor (CCR)4 were consistently detected in the circulation of healthy subjects, whereas numbers of cells expressing CCR3 were much lower. While all CCR4+ cells were T cells, a small proportion of CRT...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200010)30:10<2972::AID-IMMU2972>
更新日期:2000-10-01 00:00:00
abstract::An effective immune response against the intracellular pathogen Mycobacterium tuberculosis is strictly dependent on T cell activation. Although this protective response mainly depends on local release of pro-inflammatory cytokines by Th1 CD4(+) T cells, contribution of Vgamma9 / Vdelta2 T lymphocytes to immune protect...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(200005)30:5<1512::AID-IMMU
更新日期:2000-05-01 00:00:00
abstract::Epidermal keratinocytes differentiate and form a multilayered epidermis, which is the primary barrier between the body and the outer environment. As the epidermis is constantly exposed to a variety of microbial pathogens, its function of resisting microbial pathogens is vital. This characteristic feature is formed dur...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425827
更新日期:2005-06-01 00:00:00
abstract::The interaction of polymorphonuclear leukocytes (PMN) with the vascular endothelium and their subsequent extravasation to the tissues is a key step during different physiological and pathological processes. In certain of these pathologies the oxygen tension becomes very low, leading to reduced cellular oxidative statu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270818
更新日期:1997-08-01 00:00:00
abstract::Prostein is a prostate tissue-specific protein that is uniquely and abundantly expressed in normal and cancerous prostate tissues. Due to this expression profile, we examined the immunogenicity of prostein as a potential vaccine candidate for prostate cancer. To determine the presence of CD8 T cells specific for natur...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324768
更新日期:2004-04-01 00:00:00
abstract::A cell surface lectin found on activated human lymphoid cells has been identified and characterized using membrane glycoprotein micelles as probes. These micelles, which are large, water-soluble aggregates, are composed of glycoproteins isolated from detergent-solubilized membranes of human B lymphoblastoid cell lines...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830120708
更新日期:1982-07-01 00:00:00
abstract::Tumor necrosis factor (TNF) is one of the key primary response genes in the immune system that can be activated by a variety of stimuli. Previous analysis of chromatin accessibility to DNaseI demonstrated open chromatin conformation of the TNF proximal promoter in T cells. Here, using chromatin probing with restrictio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201243297
更新日期:2014-01-01 00:00:00
abstract::The nonobese diabetic (NOD) mouse is a relevant model for studying human insulin-dependent diabetes mellitus (IDDM). The selective destruction of insulin-secreting cells in this model is subsequent to an autoimmune reaction directed towards the beta cells inside the islets of Langerhans of the pancreas. Given the key ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210511
更新日期:1991-05-01 00:00:00
abstract::The role of CD8(+) T cells in the development of allergic airway disease is controversial. On the one hand, CD8(+) T cells are known to inhibit the development of airway hyperreactivity (AHR) in murine models of asthma. In humans, IL-10-producing CD8(+) T cells were shown to act as regulatory cells, inhibiting both pr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324623
更新日期:2004-07-01 00:00:00
abstract::In this report we investigated the production and role of interleukin (IL)2 and IL4 in the generation of antigen-specific cytotoxic T cells (CTL). We used as our model the ultraviolet-light-induced epithelial tumor 1591, a highly immunogenic regressor tumor which evokes a strong cell-mediated immune response leading t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210813
更新日期:1991-08-01 00:00:00
abstract::CD16 is an IgG Fc receptor that is predominantly expressed on human natural killer (NK) cells and granulocytes. The CD16 antigen expressed on neutrophils is a 50 to 70-kDa glycoprotein attached to the plasma membrane by a phosphatidylinositol-glycan linkage that is susceptible to cleavage by phosphatidylinositol-speci...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190431
更新日期:1989-04-01 00:00:00
abstract::In this study, we examined which cell population contributes to IL-5 production by Peyer's patch (PP) cells. Thy1.2(-) fraction of PP cells, but not those of splenocytes, secreted IL-5 in response to IL-2. We found that CD3epsilon(-)IL-2Ralpha(+) cells purified from the Thy1.2(-)B220(-) fraction of PP cells secreted I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324696
更新日期:2004-07-01 00:00:00