One cause for the apparent inability of human T cell clones to function as professional superantigen-presenting cells is autoactivation.


:Human T cell clones (TCC) are antigen-presenting cells (APC) able to present peptides and superantigens (SAg) and to process and present intact proteins. TCC express major histocompatibility complex (MHC) class II antigens and molecules involved in the accessory signal delivery, such as B7.1 and B7.2/B70. Notwithstanding these observations, the role of professional APC has been often denied to T cells because anergy of responder T cells rather than proliferation has been observed following the TCC presentation in the absence of added professional APC. Here, we show that upon stimulation with free SAg, TCC undergo proliferative responses followed, after a 1-week culture, by an SAg-dependent unresponsiveness to T cell receptor (TCR)-mediated stimuli, but not to interleukin-2. The anergy induced by the SAg can not be prevented by the addition of autologous Epstein-Barr virus (EBV)-transformed B cells, indicating that the induction of anergy occurs also in the presence of conventional APC. Conversely, if the TCC are stimulated by SAg-prepulsed irradiated APC, either EBV and TCC, the induction of anergy is not observed. After a 1-week culture, in fact, TCC stimulated with APC-bound SAg responded to TCR-mediated stimuli, irrespective of the APC (EBV or TCC) used for the SAg presentation. Stimulation of TCC with free SAg in a semisolid medium that prevents T-T cell contacts resulted in an activation followed by a state of anergy, suggesting that anergy is the consequence of SAg recognition at the single T cell level. These data indicate that the anergy observed in TCC upon a 1-week culture in the presence of soluble SAg is not the result of an inherent inability of TCC to act as professional APC. Rather the phenomenon depends on the presence of soluble SAg, leading to T cell autostimulation.


Eur J Immunol


Nisini R,Fattorossi A,Ferlini C,D'Amelio R




Has Abstract


1996-04-01 00:00:00












  • Human CD8+ T cell blasts are more sensitive than CD4+ T cell blasts to regulation by APO2L/TRAIL.

    abstract::The mechanisms responsible for the down-modulation of the activation of separated CD4(+) or CD8(+) human T cell blasts were studied using cells obtained from healthy donors. In the presence of IL-2, human CD8(+) T cell blasts were more sensitive than CD4(+) T cell blasts to regulation by APO2 ligand/TNF-related apopto...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Bosque A,Pardo J,Martínez-Lorenzo MJ,Lasierra P,Larrad L,Marzo I,Naval J,Anel A

    更新日期:2005-06-01 00:00:00

  • Th1/Th2 cytokine gene expression after mercuric chloride in susceptible and resistant rat strains.

    abstract::Mercuric chloride (HgCl2) has contrasting effects on different rat strains: susceptible strains, e.g. Brown Norway (BN) develop polyclonal B cell activation, multiple autoantibodies and widespread tissue injury. Lewis (LEW) rats are resistant: no autoimmune response occurs after HgCl2; instead, there is immunosuppress...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Gillespie KM,Saoudi A,Kuhn J,Whittle CJ,Druet P,Bellon B,Mathieson PW

    更新日期:1996-10-01 00:00:00

  • Oligodeoxynucleotide inhibition of Toll-like receptors 3, 7, 8, and 9 suppresses cytokine production in a human rheumatoid arthritis model.

    abstract::Toll-like receptors (TLRs) are innate immune receptors that respond to both exogenous and endogenous stimuli and are suggested to contribute to the perpetuation of chronic inflammation associated with rheumatoid arthritis (RA). In particular, the endosomal TLRs 3, 7, 8, and 9 have more recently been postulated to be o...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Sacre S,Lo A,Gregory B,Stephens M,Chamberlain G,Stott P,Brennan F

    更新日期:2016-03-01 00:00:00

  • Epitope-specific regulation of the T cell repertoire: carrier recognition in association with I-E or I-A does not influence the restriction of hapten-specific T cells.

    abstract::The T cell repertoire of BALB/c mice contains clones capable of recognizing p-azobenzenearsonate (ABA)-tyrosine (Tyr) in association with both I-A and I-E-encoded class II molecules. Immunization of BALB/c animals with ABA-GAT (terpolymer of L-Glu60-L-Ala30-L-Tyr10) or ABA-GLT (terpolymer of L-Glu51-L-Lys34-L-Tyr15) i...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Trannoy E,Regnier D,Campbell H,Seman M

    更新日期:1985-12-01 00:00:00

  • Surface expression by mononuclear phagocytes of an epitope shared with mycobacterial heat shock protein 60.

    abstract::Bone marrow-derived macrophages were stained with a variety of monoclonal antibodies (mAb) with specificity for the mycobacterial heat shock protein (hsp) 60 or with specific rabbit antiserum raised against mycobacterial hsp 60. The mAb ML30 as well as the specific antiserum brightly stained bone marrow-derived macrop...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Wand-Württenberger A,Schoel B,Ivanyi J,Kaufmann SH

    更新日期:1991-04-01 00:00:00

  • Characterization of type II intracellular IL-1 receptor antagonist (IL-1ra3): a depot IL-1ra.

    abstract::Three molecular forms of the IL-1 receptor antagonist (IL-1ra) have been identified and cloned. Secreted IL-1ra (sIL-1ra or IL-1ra1) contains a classical leader peptide giving a released mature protein. Two intracellular isoforms, icIL-1ra type I (IL-1ra2) and icIL-1ra type II (IL-1ra3), have no leader sequence, thus ...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Muzio M,Polentarutti N,Facchetti F,Peri G,Doni A,Sironi M,Transidico P,Salmona M,Introna M,Mantovani A

    更新日期:1999-03-01 00:00:00

  • A synthetic HIV-1 Tat protein breaches the skin barrier and elicits Tat-neutralizing antibodies and cellular immunity.

    abstract::The HIV-1 Tat protein plays a critical role in the pathogenesis of HIV and has been considered as a candidate vaccine antigen. In an effort to design a non-invasive vaccination strategy against HIV-1 that stimulates the induction of systemic and mucosal immune responses, we studied the transcutaneous delivery of a syn...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Partidos CD,Moreau E,Chaloin O,Tunis M,Briand JP,Desgranges C,Muller S

    更新日期:2004-12-01 00:00:00

  • The multifaceted Foxp3fgfp allele enhances spontaneous and therapeutic immune surveillance of cancer in mice.

    abstract::It is well established that therapeutic impairment of Foxp3+ Treg in mice and humans favors immune rejection of solid tumors. Less explored is the impact Foxp3 allelic variants may have on tumor incidence, progression and therapy. In this work, we tested and demonstrate that the Foxp3fgfp reporter allele, found previo...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Almeida-Santos J,Bergman ML,Amendoeira Cabral I,Correia V,Caramalho Í,Demengeot J

    更新日期:2020-03-01 00:00:00

  • Targeting the tumor microenvironment and T cell metabolism for effective cancer immunotherapy.

    abstract::The successful implementation of immunotherapies has provided new impetus in the fight against cancer. Antibody-mediated blockade of immune checkpoint molecules PD-1/PD-L1 and CTLA-4 has had a dramatic impact upon the treatment of previously intractable cancers such as malignant melanoma, while adoptive cell therapies...

    journal_title:European journal of immunology

    pub_type: 杂志文章,评审


    authors: Hope HC,Salmond RJ

    更新日期:2019-08-01 00:00:00

  • Cyclosporin A inhibits T cell receptor-induced interleukin-2 synthesis of human T lymphocytes by selectively preventing a transmembrane signal transduction pathway leading to sustained activation of a protein kinase C isoenzyme, protein kinase C-beta.

    abstract::Stimulation of human peripheral blood lymphocytes via T cell receptor/CD3 complex resulted in a bimodal activation of protein kinase(s) C (PKC). Within 10 min of stimulation PKC-alpha was translocated to, and thus activated in, the plasma membranes of human lymphocytes, followed by a fast dissociation of this isotype ...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Szamel M,Bartels F,Resch K

    更新日期:1993-12-01 00:00:00

  • Transcript profiling of CD16-positive monocytes reveals a unique molecular fingerprint.

    abstract::CD16-positive (CD14(++) CD16(+) and CD14(+) CD16(++) ) monocytes have unique features with respect to phenotype and function. We have used transcriptional profiling for comparison of CD16-positive monocytes and classical monocytes. We show herein that 187 genes are greater than fivefold differentially expressed, incl...

    journal_title:European journal of immunology

    pub_type: 临床试验,杂志文章


    authors: Frankenberger M,Hofer TP,Marei A,Dayyani F,Schewe S,Strasser C,Aldraihim A,Stanzel F,Lang R,Hoffmann R,Prazeres da Costa O,Buch T,Ziegler-Heitbrock L

    更新日期:2012-04-01 00:00:00

  • Defective interleukin 2 receptor expression is associated with the T cell disfunction subsequent to bone marrow transplantation.

    abstract::In the present work we have used monoclonal antibodies (mAb) as probes to attempt a dissection of the mechanisms underlying the immunodeficiency subsequent to bone marrow transplantation (BMT). To this end we have studied 19 allogeneic BMT recipients, analyzing the proliferative response of peripheral blood mononuclea...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: López-Botet M,De Landazuri MO,Izquierdo M,Ramirez A,Cámara R,Fernández-Rañada J

    更新日期:1987-08-01 00:00:00

  • Intranasal immunization with liposomes induces strong mucosal immune responses in mice.

    abstract::BALB/c mice were immunized intranasally with either soluble ovalbumin (OVA) or OVA entrapped in liposomes. The effect of adding Sigma cholera toxin B subunit (sCT-B), which contained low amounts of cholera holotoxin (CT), or recombinant CT-B (rCT-B) which was free from CT, as mucosal adjuvants was also investigated. T...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Vadolas J,Davies JK,Wright PJ,Strugnell RA

    更新日期:1995-04-01 00:00:00

  • N-terminal amino acid sequence of a human delta-chain myeloma protein.

    abstract::The N-terminal amino acid sequence (26 residues) of a delta-chain of a human myeloma (Er I) is homologous with the prototype sequence of the human VHIII subgroup. This indicates that the delta-chain is using for its V region the same pool of V genes as the rest of the immunoglobulin classes. Implications of this findi...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Milstein CP,Northrop F

    更新日期:1976-03-01 00:00:00

  • Preservation of the helper and suppressor/cytotoxic T-lymphocyte phenotype in continuous culture.

    abstract::The objective of the present investigation was to culture helper or suppressor/cytotoxic T cell subsets after extensive purification in interleukin 2-containing medium to determine whether phenotype changes for OKT4/91d6- or OKT8/Leu-2a-defined antigens do occur with prolonged culture. Repeated and sequential analysis...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Gramatzki M,Baum W,Burmester GR,Kalden JR

    更新日期:1984-08-01 00:00:00

  • Critical role of antigen-specific antibody in experimental autoimmune encephalomyelitis induced by recombinant myelin oligodendrocyte glycoprotein.

    abstract::The role of B cells and antibody in the pathogenesis of experimental autoimmune encephalomyelitis (EAE) remains controversial. We previously demonstrated that B cells are required for EAE to be induced by the 120-amino acid extracellular domain of myelin oligodendrocyte glycoprotein (MOG). In the present study, the ro...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Lyons JA,Ramsbottom MJ,Cross AH

    更新日期:2002-07-01 00:00:00

  • FK506 binding protein 12 mediates sensitivity to both FK506 and rapamycin in murine mast cells.

    abstract::The immunosuppressive drugs FK506 and rapamycin bind to a family of intracellular proteins termed FK506-binding proteins (FKBP). FK506 and rapamycin inhibit lymphocyte-activation pathways by forming complexes with an FKBP; subsequently, the drug/FKBP complexes interact with target molecules involved in signal transduc...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Fruman DA,Wood MA,Gjertson CK,Katz HR,Burakoff SJ,Bierer BE

    更新日期:1995-02-01 00:00:00

  • Target structure for natural killer cells: evidence against a unique role for transferrin receptor.

    abstract::The transferrin receptor (TfR) was recently proposed as putative natural killer (NK) cell target structure. Here data are presented against this hypothesis and it is shown that low TfR expression and high NK sensitivity can occur concommitantly . K562 cells were studied at various stages of cell proliferation. No chan...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Dokhélar MC,Garson D,Testa U,Tursz T

    更新日期:1984-04-01 00:00:00

  • The in vitro immune response to a T-independent antigen. I. The effect of macrophages and 2-mercaptoethanol.

    abstract::The in vitro immune response of murine spleen cells to the T cell-independent antigen, 2,4-dinitrophenyl-beta-alanyl-glycyl-glycyl-N(2-aminoethyl)carbamyl-methylated Ficoll (DAGG-Ficoll), requires the presence of macrophages, or 24-hr culture supernates from peritoneal cells. Complete reconstitution of the response by...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Nordin AA

    更新日期:1978-11-01 00:00:00

  • The invariant chain induces compact forms of class II molecules localized in late endosomal compartments.

    abstract::The invariant chain (Ii) binds to newly synthesized major histocompatibility complex (MHC) class II molecules and is targeted to an acidic compartment where it is degraded. To evaluate its role on the conformation and the subcellular distribution of murine MHC class II molecules we have established stable L cell trans...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Humbert M,Raposo G,Cosson P,Reggio H,Davoust J,Salamero J

    更新日期:1993-12-01 00:00:00

  • Treatment and management of primary antibody deficiency: German interdisciplinary evidence-based consensus guideline.

    abstract::This evidence-based clinical guideline provides consensus-recommendations for the treatment and care of patients with primary antibody deficiencies (PADs). The guideline group comprised 20 clinical and scientific expert associations of the German, Swiss, and Austrian healthcare system and representatives of patients. ...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Hanitsch L,Baumann U,Boztug K,Burkhard-Meier U,Fasshauer M,Habermehl P,Hauck F,Klock G,Liese J,Meyer O,Müller R,Pachlopnik-Schmid J,Pfeiffer-Kascha D,Warnatz K,Wehr C,Wittke K,Niehues T,von Bernuth H

    更新日期:2020-10-01 00:00:00

  • Differential NKG2D binding to highly related human NKG2D ligands ULBP2 and RAET1G is determined by a single amino acid in the alpha2 domain.

    abstract::The activating NK cell receptor NKG2D binds to numerous stress-induced cell surface glycoproteins with MHC class I-related ectodomains. In humans, NKG2D ligands (NKG2DL) are members of the MHC-encoded MIC and non-MHC-encoded ULBP families of proteins. The redundancy of NKG2DL raises questions about unique features ass...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Wittenbrink M,Spreu J,Steinle A

    更新日期:2009-06-01 00:00:00

  • Somatically mutated IgG anti-DNA antibody clonally related to germ-line encoded IgM anti-DNA antibody.

    abstract::Among 15 anti-DNA antibody-producing hybridomas derived from a single NZB X NZW F1 mouse, an IgM and an IgG were shown to use the same VH gene of the Q52 family. Using a combination of two primers both consisting of a mixture of oligonucleotides (one complementary to the 5' end of VH segment and one to the 3' end of V...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Taki S,Hirose S,Kinoshita K,Nishimura H,Shimamura T,Hamuro J,Shirai T

    更新日期:1992-04-01 00:00:00

  • Analysis of the T cell repertoire for myelin basic protein in thymus-grafted and other types of chimera: evidence that major histocompatibility complex molecules on accessory cells rather than T cell specificity mainly regulate susceptibility to autoimmun

    abstract::Experimental autoimmune encephalomyelitis (EAE) is a T cell-mediated autoimmune disease inducible in rodents by immunization of brain-specific antigens such as myelin basic protein (MBP). It is also well known that various strains of rats differ in their susceptibility to EAE upon active immunization. To elucidate the...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Matsumoto Y,Kawai K,Fujiwara M

    更新日期:1990-09-01 00:00:00

  • Scheduled kinetics of cell proliferation and phenotypic changes during immature thymocyte generation.

    abstract::Precursor CD4-CD8- (DN) thymocytes rearrange their TCR-beta genes, and only those which succeed in beta-selection subsequently expand and differentiate into immature CD4+CD8+ (DP) thymocytes. The cell subsets corresponding to the successive steps of this transition can be defined in terms of CD44 and CD25 expression. ...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Vasseur F,Le Campion A,Pénit C

    更新日期:2001-10-01 00:00:00

  • FcγRI (CD64): an identity card for intestinal macrophages.

    abstract::Macrophages are becoming increasingly recognized as key cellular players in intestinal immune homeostasis. However, differentiating between macrophages and dendritic cells (DCs) is often difficult, and finding a specific phenotypic signature for intestinal macrophage identification has remained elusive. In this issue ...

    journal_title:European journal of immunology

    pub_type: 评论,杂志文章


    authors: De Calisto J,Villablanca EJ,Mora JR

    更新日期:2012-12-01 00:00:00

  • Rewiring cellular metabolism via the AKT/mTOR pathway contributes to host defence against Mycobacterium tuberculosis in human and murine cells.

    abstract::Cells in homeostasis metabolize glucose mainly through the tricarboxylic acid cycle and oxidative phosphorylation, while activated cells switch their basal metabolism to aerobic glycolysis. In this study, we examined whether metabolic reprogramming toward aerobic glycolysis is important for the host response to Mycoba...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Lachmandas E,Beigier-Bompadre M,Cheng SC,Kumar V,van Laarhoven A,Wang X,Ammerdorffer A,Boutens L,de Jong D,Kanneganti TD,Gresnigt MS,Ottenhoff TH,Joosten LA,Stienstra R,Wijmenga C,Kaufmann SH,van Crevel R,Netea MG

    更新日期:2016-11-01 00:00:00

  • Glycosylphosphatidylinositol-anchored H-2Db molecules are defective in antigen processing and presentation to cytotoxic T lymphocytes.

    abstract::Glycosylphosphatidylinositol-anchored (GPI)-Db molecules are defective in mediating cytotoxic T lymphocytes (CTL) lysis of transfected lymphoma cells, compared to their transmembrane (TM) counterpart. This defect is manifest when antigenic peptide must be processed and presented through the endogenous pathway. These s...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Cariappa A,Flyer DC,Rollins CT,Roopenian DC,Flavell RA,Brown D,Waneck GL

    更新日期:1996-09-01 00:00:00

  • Mast cell heterogeneity: two-dimensional gel electrophoretic analyses of rat peritoneal and intestinal mucosal mast cells.

    abstract::Peritoneal mast cells (PMC) and intestinal mucosal mast cells (IMMC) were purified from rats infected with the nematode Nippostrongylus brasiliensis. Overall protein constituents of both mast cell subtypes were analyzed by two-dimensional gel electrophoresis using either nonequilibrium pH gradient electrophoresis (NEP...

    journal_title:European journal of immunology

    pub_type: 杂志文章


    authors: Abe T,Swieter M,Imai T,Hollander ND,Befus AD

    更新日期:1990-09-01 00:00:00

  • Thymocyte death by neglect: contribution of engulfing macrophages.

    abstract::The thymus provides the microenvironment in which thymocytes develop into mature T cells, and interactions with thymic stromal cells are thought to provide the necessary signals for thymocyte maturation. Recognition of self-MHC by T cells is a basic requirement for mature T-cell functions, and those thymocytes that do...

    journal_title:European journal of immunology

    pub_type: 杂志文章,评审


    authors: Szondy Z,Garabuczi É,Tóth K,Kiss B,Köröskényi K

    更新日期:2012-07-01 00:00:00