Abstract:
:We have analyzed the inducibility of protein kinase C (PKC)-dependent expression of CD 69 molecules in T cell receptor (TCR) transgenic thymocytes developing in the presence or absence of selecting, class I major histocompatibility complex (MHC) molecules. Small CD4+8+ thymocytes developing in the absence of selecting MHC molecules could not be induced to express CD 69 by TCR cross-linking even after spontaneous in vitro up-regulation of their TCR level which resulted in enhanced Ca++ flux. In contrast, a small proportion of CD4+8+TCRlow and most TCRhigh (CD4+8+ and CD4-8+) thymocytes developing in the presence of selecting MHC ligands could be induced to express CD 69 upon TCR cross-linking. Unlike the anti-TCR antibody, phorbol 12-myristate 13-acetate--a direct activator of PKC--induced the expression of CD 69 on all thymocytes. These results suggest that positive selection of CD4+8+ thymocytes results on coupling of TCR-mediated signals to the CD 69 expression pathway. In vitro analysis of thymocytes before and after positive selection suggests that (1) positive selection does not immediately result in resistance to deletion and (2) that sustained TCR ligation is needed to promote maturation of positively selected CD4+8+ thymocytes resulting in gradual loss of the sensitivity to deletion and acquisition of the ability to proliferate in response to TCR-mediated signals.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Swat W,Dessing M,von Boehmer H,Kisielow Pdoi
10.1002/eji.1830230326subject
Has Abstractpub_date
1993-03-01 00:00:00pages
739-46issue
3eissn
0014-2980issn
1521-4141journal_volume
23pub_type
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journal_title:European journal of immunology
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