Abstract:
:T lymphocyte subsets expressing either T cell receptor alpha/beta or gamma/delta were selected from human peripheral blood T cells and proliferative responses to molecular mass-fractionated mycobacterial lysates were determined. alpha/beta T cells primarily responded to fractions greater than 30 kDa whereas gamma/delta T cells preferentially reacted to fractions less than 3 kDa. Protease digestion abolished the stimulating activities for alpha/beta T cells, confirming that alpha/beta T cells respond to protein components. In contrast, components recognized by gamma/delta T cells proved resistant to protease digestion. In limiting dilution studies, frequencies of proliferating gamma/delta T cells remained virtually unaltered by protease treatment of stimulating lysates, while those of alpha/beta T cells became almost undetectable. Furthermore, only few gamma/delta T cells responded to the 65-kDa heat-shock protein. Our data indicate that, unlike alpha/beta T cells, gamma/delta T cells respond to mycobacterial components which are resistant to vigorous protease digestion.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Pfeffer K,Schoel B,Gulle H,Kaufmann SH,Wagner Hdoi
10.1002/eji.1830200534subject
Has Abstractpub_date
1990-05-01 00:00:00pages
1175-9issue
5eissn
0014-2980issn
1521-4141journal_volume
20pub_type
杂志文章abstract::A detailed restriction map of a 430-kb contig comprising the single Ckappa, the 5 Jkappa and the adjoining 22 Vkappa gene segments is presented. The first 12 Vkappa genes following the JkappaCkappa region belong to the Vkappa21 family, the subsequent ones to the closely related families Vkappa8 and Vkappal 9/ 28. Prev...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199805)28:05<1458::AID-IMM
更新日期:1998-05-01 00:00:00
abstract::The relationship between immunogenicity of Shigella paradysenteriae, the branched synthetic polypeptide poly-L (Tyr, Glu)-polylpro-polyllys [(T, G)-Pro--L] and human albumin (HSA) interacting with macrophages and kinetics of antigen degradation and degree of binding to the cell surface was studied. Following thioglyco...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830061107
更新日期:1976-11-01 00:00:00
abstract::The problem of the structural relationship between suppressive IgG-binding factor and low-affinity receptors for the Fc portion of IgG (Fc gamma RII) has not yet been solved. In the present work we have isolated a recombinant soluble Fc gamma RII containing only the two external domains of Fc gamma RII, and analyzed i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191213
更新日期:1989-12-01 00:00:00
abstract::T-cell activation and the subsequent transformation of activated T cells into T-cell blasts require profound changes in cell volume. However, the impact of cell volume regulation for T-cell immunology has not been characterized. Here we studied the role of the cell-volume regulating osmolyte transporter Taut for T-cel...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2012-04-01 00:00:00
abstract::Despite the presence of tumor-specific effector cells in the circulation of cancer patients, the immune response of the majority of these patients is not sufficient to prevent the growth and spread of their tumors. That tumor cells can be killed in vitro by tumor-reactive cytotoxic T cells is testimony to the fact tha...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200424938
更新日期:2004-06-01 00:00:00
abstract::Recurrent respiratory tract infections (RRTIs) are the first leading cause of community- and nosocomial-acquired infections. Antibiotics remain the mainstay of treatment, enhancing the potential to develop antibiotic resistances. Therefore, the development of new alternative approaches to prevent and treat RRTIs is hi...
journal_title:European journal of immunology
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doi:10.1002/eji.201747024
更新日期:2018-01-01 00:00:00
abstract::5'-Nucleotidase/CD73 is a key enzyme in the regulation of purinergic signaling, hydrolyzing extracellular AMP to produce adenosine, which is critical in the blood vascular system and in immunosuppression. CD73 is expressed by both blood endothelial cells and lymphatic endothelial cells. Although the role of CD73 on bl...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
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更新日期:2015-02-01 00:00:00
abstract::Only activated and effector memory T cells are thought to access non-lymphoid tissues. In contrast, naive T cells are thought to circulate only between the blood, lymph and secondary lymphoid organs. We examined the phenotype of endogenous T cells in various non-lymphoid organs and showed that a subset of cells exhibi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535539
更新日期:2006-06-01 00:00:00
abstract::The monoclonal antibody OKT3 can induce T suppressor cells of the in vitro antibody response when used at concentrations as low as 10 ng/ml and in cultures containing as few as 4% OKT3-pretreated T cells. These suppressor cells are radioresistant, cyclosporin sensitive and belong to the T4 as well as the T8 subpopulat...
journal_title:European journal of immunology
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doi:10.1002/eji.1830150504
更新日期:1985-05-01 00:00:00
abstract::We recently reported that differentiation of CD8(+) T cells from the naïve to the effector state involves the upregulation of glucose-dependent metabolism. Glucose deprivation or inhibition of glycolysis by 2-deoxy-D-glucose (2-DG) selectively inhibited production of IFN-gamma but not of IL-2. To determine a more glob...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838289
更新日期:2008-09-01 00:00:00
abstract::Protein kinase C (PKC) has been implicated in the signaling of a number of cellular responses including activation of T cells. In the present report we have evaluated the effect of increased cAMP levels on PKC activation after stimulation of two distinct receptor systems on normal human T cells. PKC substrate phosphor...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190622
更新日期:1989-06-01 00:00:00
abstract::Mice carrying the H-2b haplotype (e.g., inbred strains C57BL/6 and C57BL/10) are low responders to sperm whale myoglobin when tested in the T cell proliferation assay. Their response is improved by the removal of the Ly-2+ cells from the lymph node population, but it still remains significantly lower than that of cell...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180905
更新日期:1988-09-01 00:00:00
abstract::Biopsy specimens were obtained from the bronchial or the nasal mucosa of three patients with grass pollen-induced bronchial asthma or rhinitis 48 h after positive bronchial or nasal provocation test with grass pollen extract. T cell clones (TCC), derived from these and control specimens, were then assessed for their p...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230707
更新日期:1993-07-01 00:00:00
abstract::A mouse T cell hybrid specific for pigeon cytochrome c in the context of I-Ek responds by secreting interleukin 2 when co-cultured with the native antigen and the B cell lymphoma, LK-35.2, or naive splenic B cells as antigen-presenting cells (APC). Cytochromes c and their corresponding C-terminal fragments which are n...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160702
更新日期:1986-07-01 00:00:00
abstract::Dendritic cells (DC) are unique in their ability to present antigen to naive T cells, and therefore play a central role in the initiation of immune responses. Characterization of DC-specific genes may help to unravel the mechanism underlying their potent antigen presenting capacity. Here we describe the identification...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200012)30:12<3585::AID-IMMU3585>
更新日期:2000-12-01 00:00:00
abstract::NK cells play important roles in immune surveillance against malignancy and virus-infected cells. NK cell functions are affected in patients infected with HIV-1; however, whether there is direct interaction between NK cells and HIV-1 remains controversial. In this study the expression of CD4, an important receptor for...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737217
更新日期:2007-08-01 00:00:00
abstract::The crucial role of interleukin (IL)-4 in the induction of murine IgG1 and IgE responses, which are coupled through the process of sequential isotype switching, has been well documented. Whereas IL-4 is obligatory for the induction of IgE responses, it enhances IgG1 responses. In this study, using neutralizing antibod...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240624
更新日期:1994-06-01 00:00:00
abstract::We have characterized the peripheral B cell repertoire in T cell-mediated insulin-dependent diabetes mellitus (IDMM) and in B cell-mediated autoimmune idiopathic thrombocytopenic purpura (AITP). The VH6-containing repertoire in adult patients with IDDM or AITP and healthy control subjects was investigated by PCR ampli...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199909)29:09<2853::AID-IMM
更新日期:1999-09-01 00:00:00
abstract::Mucosal boosting of BCG-immunised individuals with a subunit tuberculosis (TB) vaccine would be highly desirable, considering that the lungs are the principal port of entry for Mycobacterium tuberculosis (MTB) and the site of the primary infection and reactivation. However, the main roadblock for subunit TB vaccine de...
journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535325
更新日期:2006-01-01 00:00:00
abstract::Stimulation of T cells via the T cell receptor (TCR) leads to an increase intracellular in free Ca2+ levels ([Ca2+]i) and the activation of the MAP kinase signaling pathway. This study analyzes for the first time Ca2+ fluxes in naive cytotoxic T cells stimulated with full agonists, partial agonists, or antagonists. Fo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271241
更新日期:1997-12-01 00:00:00
abstract::Stem cell factor (SCF) is encoded at the Sl locus of the mouse and is the ligand for the c-kit receptor. Recombinant rat SCF164 (rrSCF164) induces proliferation and promotes maturation of mouse mast cells in vitro and in vivo and can also induce c-kit receptor-dependent mouse mast cell degranulation. We now report tha...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1993-04-01 00:00:00
abstract::Human NK cells can be subdivided into CD56(dim) and CD56(bright) NK cells, which exhibit different phenotypical and functional characteristics. As murine NK cells lack CD56 or a distinct correlate, direct comparative studies of NK cells in mice and humans are limited. Although CD27 is currently proposed as a feasible ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200940056
更新日期:2010-05-01 00:00:00
abstract::On the premise that an individual with an intact immune system has the capability to develop both cellular and antibody immune responses supported by the balance between the lymphokines secreted by T helper (Th) cells, we studied the interaction between different types of Th cell clones in vivo and the parameters that...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250312
更新日期:1995-03-01 00:00:00
abstract::We have previously identified a molecule on the T cell surface, which, in addition to CD2 is involved in the rosette phenomenon. This is a 32-kDa single polypeptide chain which we have termed E2. The studies reported here show striking patterns on the glycosylation status of E2. It is a heavily sialylated and glycosyl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190813
更新日期:1989-08-01 00:00:00
abstract::To investigate the role of membrane lymphotoxin (LT)alpha1 / beta2 and its LTbeta receptor (LTbetaR) in the protective immune response to Mycobacterium bovis bacillus Calmette-Guérin (BCG) infection, we have used a soluble fusion molecule (LTbetaR-IgG1). LTbetaR-Ig treatment interferes with granuloma formation mainly ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199912)29:12<4002::AID-IMM
更新日期:1999-12-01 00:00:00
abstract::Helicobacter pylori is a Gram-negative bacterium that causes a variety of gastrointestinal diseases, such as duodenal ulcer and gastric carcinoma. The T cell response against H. pylori is thought to contribute to the pathogenesis of these diseases. Here, we show that mouse-adapted H. pylori is able to polyclonally act...
journal_title:European journal of immunology
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830241145
更新日期:1994-11-01 00:00:00
abstract::Human cytomegalovirus (HCMV) employs multiple mechanisms to evade the immune system and succeeds to persist lifelong in the host. Human dendritic cells (DC) are the main antigen-presenting cells and play the key role in inducing and maintaining immune responses. Here, we studied the interaction of HCMV with DC. We fou...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323612
更新日期:2003-06-01 00:00:00