CD4+CD25+ T cells as immunoregulatory T cells in vitro.

Abstract:

:We have further characterized the in vitro phenotype and function of anergic and suppressive CD4(+)25(+) T cells. Following TCR ligation, DO.11.10 CD4(+)25(+) T cells suppress the activation of OT-1 CD8(+)25(-) T cells in an antigen nonspecific manner. Although suppression was seen when using a mixture of APC from both parental strains, it was very much more marked when using F1 APC. APC pretreated with, and then separated from CD4(+)25(+) T cells did not have diminished T cell costimulatory function, suggesting that APC are not the direct targets of CD4(+)25(+) T cell regulation. CTLA-4 blockade failed to abrogate suppression by CD4(+)25(+) T cells in mixing experiments. Although CD4(+)25(+) T cells failed to respond following cross-linking of TCR, they could be induced to proliferate following the addition of exogenous IL-2, allowing the generation of a T cell line from CD4(+)25(+) T cells. After the first in vitro restimulation, CD4(+)25(+) T cells were still anergic and suppressive following TCR engagement. However, after three rounds of restimulation, their anergic and suppressive status was abrogated.

journal_name

Eur J Immunol

authors

Chai JG,Tsang JY,Lechler R,Simpson E,Dyson J,Scott D

doi

10.1002/1521-4141(200208)32:8<2365::AID-IMMU2365>3

keywords:

subject

Has Abstract

pub_date

2002-08-01 00:00:00

pages

2365-75

issue

8

eissn

0014-2980

issn

1521-4141

journal_volume

32

pub_type

杂志文章
  • A TRAV26-1-encoded recognition motif focuses the biased T cell response in celiac disease.

    abstract::The semi-public T-cell response towards the gluten epitope DQ2.5-glia-α2 uses a prototypic TCR encoded by the germline segments TRAV26-1 and TRBV7-2. Through mutagenesis experiments, we show that a TRAV26-1encoded recognition motif contacts the MHC β-chain and the TCR CDR3β loop underpinning this conserved T-cell resp...

    journal_title:European journal of immunology

    pub_type: 信件

    doi:10.1002/eji.201948235

    authors: Frick R,Gunnarsen KS,Dahal-Koirala S,Risnes LF,Sollid LM,Sandlie I,Høydahl LS,Løset GÅ

    更新日期:2020-01-01 00:00:00

  • The controversial relationship between NLRP3, alum, danger signals and the next-generation adjuvants.

    abstract::Alum has been the only adjuvant licensed for human vaccines for decades and is still widely used, but its mechanism of action remains obscure. Recently, the NLRP3 inflammasome has been linked to the immunostimulatory properties of alum and other particulate adjuvants, although it is disputed to what degree NLRP3 is ge...

    journal_title:European journal of immunology

    pub_type: 杂志文章,评审

    doi:10.1002/eji.200940039

    authors: Spreafico R,Ricciardi-Castagnoli P,Mortellaro A

    更新日期:2010-03-01 00:00:00

  • Membrane immunoglobulins of spontaneous B lymphomas of aged BALB/c mice.

    abstract::Four cell lines derived from spontaneous BALB/c lymphoma tumors were analyzed with regard to the type of their membrane immunoglobulins (Ig). Using lactoperoxidase iodination of membrane proteins combined with immunoprecipitation and electrophoresis on polyacrylamide gel, three of these cell lines (X16c, L10A and K46)...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830110605

    authors: Laskov R,Kim JK,Woods VL,McKeever PE,Asofsky R

    更新日期:1981-06-01 00:00:00

  • Selective enhancement of B cell antigen receptor-mediated antigen presentation by treatment with transforming growth factor-beta.

    abstract::TGF-beta1 was examined for the ability to regulate Ag-presentation by B cells, using A20-HL B lymphoma cells bearing TNP-specific IgM receptors. Treatment of A20-HL cells with TGF-beta1 at 1 ng/ml, a concentration that inhibited proliferation, enhanced presentation of Ag internalized via surface IgM (sIgM), but not vi...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200324018

    authors: Arai C,Ichijo T,Tanaka Y,Okada Y,Umeda M,Uchida T,Kiniwa M,Kakiuchi T

    更新日期:2003-07-01 00:00:00

  • Immune depression in trypanosome-infected mice. III. suppressor cells.

    abstract::Spleen cells from trypanosome-infected mice strongly suppressed lymphocyte stimulation induced in normal spleen cell populations by lipopolysaccharide, concanavalin A or allogeneic (H-2-different) stimulator cells. This suppression was not H-2-restricted, as responses of spleen lymphocytes both allogeneic and syngenei...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830090306

    authors: Pearson TW,Roelants GE,Pinder M,Lundin LB,Mayor-Withey KS

    更新日期:1979-03-01 00:00:00

  • Cationic residues in pathogenic anti-DNA autoantibodies arise by mutations of a germ-line gene that belongs to a large VH gene subfamily.

    abstract::The F1 progeny of autoimmune NZB and normal SWR mice uniformly develop severe and accelerated lupus nephritis. The (SWR x NZB)F1 mice produce an oligoclonally expanded population of nephritogenic anti-DNA autoantibodies that share a recurrent cross-reactive idiotype (Id564), use highly homologous VH genes and surprisi...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830220302

    authors: O'Keefe TL,Datta SK,Imanishi-Kari T

    更新日期:1992-03-01 00:00:00

  • IgG response in vitro. I. The requirement for an intermediate responsive cell type.

    abstract::We have studied a secondary IgG anti-dinitrophenyl response in dissociated mouse spleen cell cultures which is comparable in magnitude to the responses detected after adoptive transfer. The data indicate that the responsive precursor cell is not a recirculating memory cell but is an intermediate B cell type which appe...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830060104

    authors: North JR,Askonas BA

    更新日期:1976-01-01 00:00:00

  • IL-15-IgG2b fusion protein accelerates and enhances a Th2 but not a Th1 immune response in vivo, while IL-2-IgG2b fusion protein inhibits both.

    abstract::We have explored how IL-15 influences Th1 or Th2 type immune response in vivo. Intraperitoneal application of an IL-15-IgG2b fusion protein (FP) to mice did neither significantly affect the footpad swelling nor the production of hemagglutinizing antibodies in a delayed type hypersensitivity reaction to sheep red blood...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1521-4141(199810)28:10<3312::AID-IMM

    authors: Rückert R,Herz U,Paus R,Ungureanu D,Pohl T,Renz H,Bulfone-Paus S

    更新日期:1998-10-01 00:00:00

  • "In vivo" activated splenic T cells are refractory to interleukin 2 growth "in vitro".

    abstract::Naturally activated T lymphocytes present in normal mouse spleen were studied for direct reactivity to interleukin 2 (IL 2) and for binding of anti-IL 2 receptor (IL 2R) antibodies or radiolabeled IL 2. The majority of large-sized splenic T lymphocytes are IL 2R-; thus, at the most one third of large L3T4+ T cells and...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830170702

    authors: Bandeira A,Larsson EL,Forni L,Pereira P,Coutinho A

    更新日期:1987-07-01 00:00:00

  • Some active areas of DC research and their medical potential.

    abstract::This Viewpoint series provides authoritative and detailed outlines of exciting areas of DC research. Some of the subjects that frequently come up include development of DC; distribution of DC in lymphoid and non-lymphoid tissues such as skin, intestine and lung; different forms or subsets of DC; and the role of DC in ...

    journal_title:European journal of immunology

    pub_type:

    doi:10.1002/eji.201040733

    authors: Steinman RM

    更新日期:2010-08-01 00:00:00

  • Major histocompatibility complex class I presentation of ovalbumin peptide 257-264 from exogenous sources: protein context influences the degree of TAP-independent presentation.

    abstract::Peritoneal macrophages from C57BL/6 mice process antigens from bacteria or coated on polystyrene beads for presentation by major histocompatibility complex (MHC) class I molecules. To investigate this antigen processing pathway, peritoneal macrophages from homozygous TAP1-/- mice, which lack the transporter associated...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830261135

    authors: Wick MJ,Pfeifer JD

    更新日期:1996-11-01 00:00:00

  • IL-12 selectively regulates STAT4 via phosphatidylinositol 3-kinase and Ras-independent signal transduction pathways.

    abstract::IL-12 is an important immunomodulatory cytokine that induces tyrosine phosphorylation and activation of the signal transducer and activator of transcription (STAT)4. IL-12 induces sustained activation and nuclear translocation of STAT4 and this regulatory process is coupled to both tyrosine and serine phosphorylation ...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1521-4141(200005)30:5<1425::AID-IMMU

    authors: Athié-M V,Flotow H,Hilyard KL,Cantrell DA

    更新日期:2000-05-01 00:00:00

  • p(⁷⁰S⁶K¹) in the TORC1 pathway is essential for the differentiation of Th17 Cells, but not Th1, Th2, or Treg cells in mice.

    abstract::The TORC1 pathway is necessary for ribosomal biogenesis and initiation of protein translation. Furthermore, the differentiation of Th1 and Th17 cells requires TORC1 activity. To investigate the role of the TORC1 pathway in the differentiation of Th1 and/or Th17 cells in more detail, we compared the differentiation cap...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201445422

    authors: Sasaki CY,Chen G,Munk R,Eitan E,Martindale J,Longo DL,Ghosh P

    更新日期:2016-01-01 00:00:00

  • A novel CD11c.DTR transgenic mouse for depletion of dendritic cells reveals their requirement for homeostatic proliferation of natural killer cells.

    abstract::Dendritic cells (DC) are known to support the activation of natural killer (NK) cells. However, little is known about the role for DC in NK-cell homeostasis. In order to investigate this question, a novel bacterial artificial chromosome transgenic mouse model was generated in which the diphtheria toxin receptor is exp...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200838659

    authors: Hochweller K,Striegler J,Hämmerling GJ,Garbi N

    更新日期:2008-10-01 00:00:00

  • A natural protective function of invariant NKT cells in a mouse model of innate-cell-driven lung inflammation.

    abstract::Activation of invariant natural killer T (iNKT) cells by treatment with their α-galactosyl ceramide ligand provides therapeutic benefits in several immune inflammatory settings. Given the artificial nature of this stimulation, the natural regulatory functions of iNKT remain uncertain. Addressing this issue in a mouse ...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201040647

    authors: Bourgeois EA,Levescot A,Diem S,Chauvineau A,Bergès H,Milpied P,Lehuen A,Damotte D,Gombert JM,Schneider E,Girard JP,Gourdy P,Herbelin A

    更新日期:2011-02-01 00:00:00

  • Distinct and regulated expression of Notch receptors in hematopoietic lineages and during myeloid differentiation.

    abstract::Hematopoietic development is a delicate balance of cell fate decisions in multipotent cells between self-renewal and differentiation. In multiple developmental systems, the Notch receptors are important factors regulating these processes. Hematopoietic progenitor cells have been shown to express Notch1, and studies wi...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/1521-4141(200111)31:11<3240::aid-immu3240>

    authors: Jönsson JI,Xiang Z,Pettersson M,Lardelli M,Nilsson G

    更新日期:2001-11-01 00:00:00

  • LIGHT, a new TNF superfamily member, is essential for memory T helper cell-mediated activation of dendritic cells.

    abstract::LIGHT is a recently identified member of the TNF superfamily that is up-regulated on activated T cells and can cooperate with CD40L to condition DC for the priming of CTL. In this report, we show that an intracellular pool of LIGHT is found selectively in some CD45RA-CD27+CD4+ "central" memory T cells and to a greater...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200324410

    authors: Morel Y,Truneh A,Costello RT,Olive D

    更新日期:2003-11-01 00:00:00

  • Thymic stromal organization is regulated by the specificity of T cell receptor/major histocompatibility complex interactions.

    abstract::The thymic architecture is normally compartmentalized into a central medulla surrounded by a peripheral cortical region. We investigated how compartmentalization of the thymic stroma is regulated using T cell receptor (TCR)-transgenic mouse models. Our studies show that the signals generated by TCR/peptide/major histo...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830270121

    authors: Brabb T,Huseby ES,Morgan TM,Sant'Angelo DB,Kirchner J,Farr AG,Goverman J

    更新日期:1997-01-01 00:00:00

  • Multiple subsets of HIV-specific cytotoxic T lymphocytes in humans and in mice.

    abstract::The human immunodeficiency virus type 1 (HIV-1) induces a strong cytotoxic T lymphocyte (CTL) response in humans following infection. HIV-specific CTL can be detected directly in the blood and lungs of infected patients, and can be expanded in vitro by stimulation with autologous HIV-infected lymphoblasts. Furthermore...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830190904

    authors: Chenciner N,Michel F,Dadaglio G,Langlade-Demoyen P,Hoffenbach A,Leroux A,Garcia-Pons F,Rautmann G,Guy B,Guillon JM

    更新日期:1989-09-01 00:00:00

  • Transfer of immune complexes from lymphocytes to follicular dendritic cells.

    abstract::Antigens in the form of immune complexes are retained on the membranes of follicular dendritic cells (FDC) for long periods of time. To examine how immune complexes reach germinal centers, where FDC are located, we injected mice with anti-2,4-dinitrophenyl (DNP) antibodies complexed to DNP-myoglobin-coated gold partic...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830160211

    authors: Heinen E,Braun M,Coulie PG,Van Snick J,Moeremans M,Cormann N,Kinet-Denoël C,Simar LJ

    更新日期:1986-02-01 00:00:00

  • Human interdigitating dendritic cells directly stimulate CD40-activated naive B cells.

    abstract::Human interdigitating dendritic cells (IDC) were isolated from tonsils based on their CD40+ lineage-negative expression in situ. Isolated IDC displayed a phenotypic profile similar to that of IDC in tonsils and spleen in situ, characterized by high-level expression of major histocompatibility complex class II, the co-...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830270531

    authors: Björck P,Flores-Romo L,Liu YJ

    更新日期:1997-05-01 00:00:00

  • Cyclopentenone prostaglandins induce endothelial cell apoptosis independent of the peroxisome proliferator-activated receptor-gamma.

    abstract::Cyclopentenone prostaglandins (CP-PG), such as prostaglandin A1 (PGA1) or 15-deoxy-Delta(12,14)-prostaglandin J2 (PGJ2), induce apoptosis in different cell types. PGJ2 is also a potent activator of the peroxisome proliferator-activated receptor-gamma (PPARgamma). We investigated whether PPARgamma regulates CP-PG-induc...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200324053

    authors: Erl W,Weber C,Zernecke A,Neuzil J,Vosseler CA,Kim HJ,Weber PC

    更新日期:2004-01-01 00:00:00

  • Frequency, heterogeneity and encephalitogenicity of T cells specific for myelin oligodendrocyte glycoprotein in naive outbred primates.

    abstract::Auto-reactive T cells present in healthy subjects remain in a state of unresponsiveness, but may trigger autoimmunity under various situations. Although myelin oligodendrocyte glycoprotein (MOG) is a potential target antigen in multiple sclerosis (MS), MOG-reactive T cell responses are present in the blood of both hea...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/1521-4141(2001010)31:10<2942::aid-immu2942

    authors: Villoslada P,Abel K,Heald N,Goertsches R,Hauser SL,Genain CP

    更新日期:2001-10-01 00:00:00

  • Short peptides sensitize target cells to CTL specific for the MHC class Ib molecule, H2-M3.

    abstract::The MHC class Ib molecule H2-M3 presents N-formylated peptides from the N terminus of proteins encoded by the mitochondrial genome to CTL. A panel of CTL specific for a peptide derived from a mitochondrial protein, either COI or ND1, was used to determine the optimal peptide length for sensitizing antigen-deficient ta...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1521-4141(199811)28:11<3773::AID-IMM

    authors: Dabhi VM,Lindahl KF

    更新日期:1998-11-01 00:00:00

  • Identification of a new epitope of the 4F2/44D7 molecular complex present on sarcolemma and isolated cardiac fibers.

    abstract::The murine monoclonal antibody (mAb) CB43, raised against the K-562 erythroleukemia line, reacts with monocytes, tissue macrophages, thymocytes and with all the human lines tested but not with resting lymphocytes, large granular lymphocytes, granulocytes and erythrocytes. However, activated lymphocytes and natural kil...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.1830190102

    authors: Bellone G,Alloatti G,Levi R,Geuna M,Tetta C,Peruzzi L,Letarte M,Malavasi F

    更新日期:1989-01-01 00:00:00

  • The tyrosine kinase Abl is a component of macrophage podosomes and is required for podosome formation and function.

    abstract::Myeloid leukocytes form actin-based plasma membrane protrusions, called podosomes, that are implicated in myeloid cell recruitment into tissues and cell migration within the interstitium. In this study, we show that tyrosine kinases of the Abl family are present in podosomes formed by murine and human macrophages. Sil...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201142270

    authors: Baruzzi A,Berton G

    更新日期:2012-10-01 00:00:00

  • NKG2D engagement of colorectal cancer-specific T cells strengthens TCR-mediated antigen stimulation and elicits TCR independent anti-tumor activity.

    abstract::The NKG2D receptor is expressed by human NK, gammadelta T and alpha/beta T lymphocytes and its engagement results in the stimulation of effector cells. We evaluated the role of NKG2D receptor in anti-colorectal cancer (CRC) immune response. The cell surface expression of stress-inducible NKG2D ligands MICA/B (MHC clas...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200323909

    authors: Maccalli C,Pende D,Castelli C,Mingari MC,Robbins PF,Parmiani G

    更新日期:2003-07-01 00:00:00

  • IL-33 causes selective mast cell tolerance to bacterial cell wall products by inducing IRAK1 degradation.

    abstract::Mast cells are important cellular constituents of epithelial-mesenchymal interactions, densely located at sites of microbial entry into the host where they are continuously exposed to products from commensals. In order to avoid excessive activation and the associated pathology, mast cell responses to TLR agonists must...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.201242786

    authors: Sandig H,Jobbings CE,Roldan NG,Whittingham-Dowd JK,Orinska Z,Takeuchi O,Akira S,Bulfone-Paus S

    更新日期:2013-04-01 00:00:00

  • Loss of FOXP3 expression in natural human CD4+CD25+ regulatory T cells upon repetitive in vitro stimulation.

    abstract::The adoptive transfer of CD4(+)CD25(+) natural regulatory T cells (Treg) is a promising strategy for the treatment of autoimmune diseases and the prevention of alloresponses after transplantation. Clinical trials exploring this strategy require efficient in vitro expansion of this rare cell population. Protocols devel...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200838904

    authors: Hoffmann P,Boeld TJ,Eder R,Huehn J,Floess S,Wieczorek G,Olek S,Dietmaier W,Andreesen R,Edinger M

    更新日期:2009-04-01 00:00:00

  • Categorical selection of the antibody repertoire in splenic B cells.

    abstract::In the bone marrow, the passage of developing B cells through critical checkpoints of differentiation is associated with a reduction of specific categories of CDR3 of the Ig heavy chain (CDR-H3), particularly those with excessive hydrophobic or charged amino acids and those with a length of eight or fewer residues. To...

    journal_title:European journal of immunology

    pub_type: 杂志文章

    doi:10.1002/eji.200636569

    authors: Schelonka RL,Tanner J,Zhuang Y,Gartland GL,Zemlin M,Schroeder HW Jr

    更新日期:2007-04-01 00:00:00