Abstract:
:Activation of T cells was shown to up-regulate the Fas ligand (FasL) which binds to the CD95 (APO-1/Fas) antigen and mediates activation-induced cell death (AICD) of activated T cells and T lymphoma cells. A recent report showed that mouse B cells express the FasL upon activation with lipopolysaccharide (LPS). We therefore asked whether activation of human B cells induces expression of FasL and whether AICD is mediated, as in T cells, through autocrine production of the FasL. We used human tonsillar B cells and Burkitt lymphoma cell lines which were activated by CD40 ligand, surface (s)IgM cross-linking, or LPS. Northern and Western blot analysis failed to detect FasL during B cell activation or AICD of both normal and malignant B cells. Low-level expression of FasL was detected by reverse transcriptase-polymerase chain reaction. Functional experiments, however, showed that FasL is not functionally expressed upon activation. IgM-mediated AICD in the tonsillar or Burkitt lymphoma B cells could not be inhibited by FasL blocking. Thus, our data show that, in contrast to T cells, activation of normal or malignant human B cells does not lead to functional FasL expression.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Daniel PT,Oettinger U,Mapara MY,Bommert K,Bargou R,Dörken Bdoi
10.1002/eji.1830270433subject
Has Abstractpub_date
1997-04-01 00:00:00pages
1029-34issue
4eissn
0014-2980issn
1521-4141journal_volume
27pub_type
杂志文章abstract::BALB/c mice were immunized intranasally with either soluble ovalbumin (OVA) or OVA entrapped in liposomes. The effect of adding Sigma cholera toxin B subunit (sCT-B), which contained low amounts of cholera holotoxin (CT), or recombinant CT-B (rCT-B) which was free from CT, as mucosal adjuvants was also investigated. T...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250417
更新日期:1995-04-01 00:00:00
abstract::Patients undergoing allogeneic bone marrow transplantation offer a unique system to analyze NK cell development in vivo. We analyzed NK cells from 23 such patients to assess the acquisition of activating receptors. Four patients displayed an immature NK cell surface phenotype at engraftment, as their cells were CD16(-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324668
更新日期:2004-02-01 00:00:00
abstract::Spleen cells from BALB/c mice primed with keyhole limpet hemocyanin (KLH), were stimulated with heat-killed vaccine of rough Pneumococcus pneumoniae R36A (Pn) and/or phosphorylcholine (PC)-coupled KLH to induce an anti-PC response in vitro. The response to PC-KLH was found to be T-dependent while it is T-independent t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060605
更新日期:1976-06-01 00:00:00
abstract::Lipids are key regulators of immune responses. In this study we investigated the direct impact of oxidized phospholipids (ox-PL) on T cell activation and function. We could demonstrate that ox-PL strongly inhibit proliferation of purified human T cells induced with anti-CD3/CD28 or anti-CD3/CD63 mAb, whereas prolifera...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737619
更新日期:2008-03-01 00:00:00
abstract::Major advances in preventing, delaying, or curing individual pathologies are responsible for an increasingly long life span in the developed parts of our planet, and indeed reaching eight to nine decades of life is nowadays extremely frequent. However, medical and sanitary advances have not prevented or delayed the un...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201546178
更新日期:2016-10-01 00:00:00
abstract::Immunologic control of C3 gene expression by tissue macrophages can be accomplished by treatment of spleen fragments with anti-C3 antibody. We now demonstrate that suppression of C3 requires participation of T lymphocytes of both the CD4+ and CD8+ phenotypes. Pretreatment of splenic tissue with anti-Thy-1.2 monoclonal...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221212
更新日期:1992-12-01 00:00:00
abstract::Among 15 anti-DNA antibody-producing hybridomas derived from a single NZB X NZW F1 mouse, an IgM and an IgG were shown to use the same VH gene of the Q52 family. Using a combination of two primers both consisting of a mixture of oligonucleotides (one complementary to the 5' end of VH segment and one to the 3' end of V...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220417
更新日期:1992-04-01 00:00:00
abstract::Interactions between CD8+ T cells and endothelial cells are important in both protective and pathologic immune responses. Endothelial cells regulate the recruitment of CD8+ T cells into tissues, and the activation of CD8+ T cells by antigen presentation and costimulatory signals. PD-L1 and PD-L2 are recently described...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324270
更新日期:2003-11-01 00:00:00
abstract::We have established idiotope (Id)-specific T cell lines and clones derived from at least 4 different BALB/c mice immunized with the light chain (lambda 2(315] of the BALB/c myeloma protein M315 (alpha, lambda 2). Independently derived clones were indistinguishable in that they reacted to V lambda 2(315), one or more o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830161110
更新日期:1986-11-01 00:00:00
abstract::Among human peripheral blood B cells we localized the precursors of two interleukin-dependent B cell activation processes: the specific response to a particulate antigen, trinitrophenylated polyacrylamide beads (TNP-PAA) and the polyclonally induced response to pokeweed mitogen. In both cases the precursors belong to ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830161019
更新日期:1986-10-01 00:00:00
abstract::High frequencies of CD5+TcR alpha/beta- T cells were induced in the peritoneal cavity of rats immunized with syngeneic W439 lymphoma cells. These TcR alpha/beta- cells expressed TcR delta mRNA as analyzed by the polymerase chain reaction technique. The delta + (TcR gamma/delta +) T cells were of the CD2+, CD3+, CD4-, ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830211122
更新日期:1991-11-01 00:00:00
abstract::A fraction of activated CD8+ T cells expresses CD40 ligand (CD40L), a molecule that plays a key role in T cell-dependent B cell stimulation. CD8+ T cell clones were examined for CD40L expression and for their capacity to allow the growth and differentiation of B cells, upon activation with immobilized anti-CD3. Accord...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251039
更新日期:1995-10-01 00:00:00
abstract::FTY720 is a novel immunosuppressant that improves the outcomes after solid organ and bone marrow transplantation (BMT) due to the sequestration of T cells into LN. We tested the hypothesis that the sequestration of donor T cells in LN by FTY720 would enhance their interaction with host APC, thus causing a greater degr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636123
更新日期:2007-01-01 00:00:00
abstract::TCR- but not CD2-triggered IL-2 production is p56(lck) dependent. To test the hypothesis that p59(fyn), a second src-family protein tyrosine kinase (PTK) expressed in T lymphocytes, might be an essential upstream component of the CD2 signaling pathway, we generated human (h) CD2 transgenic (tg) fyn(+/+) and fyn(-/-) m...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2000012)30:12<3507::AID-IMMU3507
更新日期:2000-12-01 00:00:00
abstract::T cell recognition of self antigens is a key event in the pathogenesis of autoimmune diseases. To date, the initial events that trigger autoreactive T cells are unknown. The "molecular mimicry" hypothesis predicts that during an infection T cells that recognize both a microbial antigen and a related self peptide becom...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200002)30:2<448::AID-IMMU448>3.0
更新日期:2000-02-01 00:00:00
abstract::Specific members of the Rho family of GTPases exert unique influences on thymocyte proliferation, differentiation and deletion. Dbs is a guanine nucleotide exchange factor which is expressed throughout thymocyte development and is able to activate the Rho family GTPases CDC42, RhoA and RhoG. Transgenic mice expressing...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324400
更新日期:2004-03-01 00:00:00
abstract::At the surface of the murine T lymphoma cell line RMA-S, the expression of "empty" class I molecules can be dramatically enhanced by culture at 26 degrees C. These class I molecules are unstable following transfer to 37 degrees C unless they are loaded with exogenously added peptides. Class I heterodimers that have fa...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220639
更新日期:1992-06-01 00:00:00
abstract::Muramyl peptides are the building blocks of bacterial peptidoglycan, and their biological functions in mammals have been extensively studied. In particular, muramyl peptides trigger inflammation, contribute to host defense against microbial infections, and modulate the adaptive immune response to antigens. These bacte...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040827
更新日期:2011-05-01 00:00:00
abstract::CSF1R (colony stimulating factor 1 receptor) is the main receptor for CSF1 and has crucial roles in regulating myelopoeisis. CSF1R can be proteolytically released from the cell surface by ADAM17 (A disintegrin and metalloprotease 17). Here, we identified CSF1R as a major substrate of ADAM17 in an unbiased degradomics ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201646482
更新日期:2016-12-01 00:00:00
abstract::Similarities between the physical structures of avian and mammalian major histocompatibility complex (MHC) class I glycoproteins have been proposed based on comparative alignment of their amino acid sequences. To investigate the physical structure of the chicken class I glycoprotein, we cloned the cDNA representing th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250740
更新日期:1995-07-01 00:00:00
abstract::Antimicrobial peptides human beta-defensins (hBD) are mainly produced by epithelia of several organs including skin, and participate in innate immunity by killing invading pathogens. Besides their microbicidal activities, hBD activate several inflammatory and immune cells. Since hBD are generated by tissues where mast...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636379
更新日期:2007-02-01 00:00:00
abstract::CD22 is a B lymphocyte-specific membrane protein that functions as an adhesion molecule via its interactions with a subset of alpha 2-6-linked sialic acid-containing glycoproteins. Engagement of CD22 with a monoclonal antibody (HB22.23) that blocks the binding of CD22 to its ligands results in rapid CD22 tyrosine phos...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260610
更新日期:1996-06-01 00:00:00
abstract::Human natural killer (NK) cells are divided into two subsets: CD56bright and CD56dim NK cells, which differ in maturation, function and distribution. Mechanisms regulating NK cell functions are not completely understood. Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor, that binds to a variet...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747289
更新日期:2018-05-01 00:00:00
abstract::The fine specificity of murine B 10.A/SgSn (B 10.A) T cells reactive with hen egg-white lysozyme (HEL) has been studied through the use of reduced, carboxymethylated HEL, a set of peptides encompassing the entire molecule, and a set of variant lysozymes from other species. Cells were taken from the lymph nodes drainin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830100705
更新日期:1980-07-01 00:00:00
abstract::To delineate the extent of O-galactosyltransferase deficiency within the lymphoid lineage, monoclonal antibody specific for the Thomsen-Friedenreich (TF) antigen (Gal beta 1----3GalNAc alpha 1-O-Ser/Thr) and its precursor the Tn antigen (GalNAc alpha 1-O-Ser/Thr) were applied to the flow cytometric analysis of periphe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220724
更新日期:1992-07-01 00:00:00
abstract::Cofilin, an actin-depolymerizing protein, is essential for the functional dynamics of the actin cytoskeleton and for cell viability. In unstimulated human peripheral blood T lymphocytes cofilin is phosphorylated and localized in the cytoplasm. Following co-stimulation through accessory receptors (e.g. CD2 or CD28) - h...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2000012)30:12<3422::AID-IMMU3422
更新日期:2000-12-01 00:00:00
abstract::The murine monoclonal antibody F23.1 reacts with an allotypic determinant on the beta chain of the T cell receptor expressed by approximately 20% of T helper and cytotoxic T lymphocytes of most common mouse strains. This IgG2a antibody, either in soluble form or covalently coupled to Sepharose beads, can activate rest...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160310
更新日期:1986-03-01 00:00:00
abstract::An atypical guanine exchange factor, Dock2 is specifically expressed in hematopoietic cells and regulates activation and migration of immune cells through activating Ras-related C3 botulinum toxin substrate (Rac). Dock2 was shown to be critical in the development of various inflammatory diseases, including allergic di...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201747157
更新日期:2018-06-01 00:00:00
abstract::Human herpesvirus 6B (HHV-6B) is a ubiquitous pathogen with frequent reactivation observed in immunocompromised patients such as BM transplant (BMT) recipients. Adoptive immunotherapy is a promising therapeutic avenue for the treatment of opportunistic infections, including herpesviruses. While T-cell immunotherapy ca...
journal_title:European journal of immunology
pub_type: 临床试验,杂志文章
doi:10.1002/eji.201444931
更新日期:2014-12-01 00:00:00
abstract::Hypoxia and glucose deprivation are often observed in the microenvironment surrounding solid tumors in vivo. However, how they interfere with MHC class I antigen processing and CD8(+) T-cell responses remains unclear. In this study, we analyzed the production of antigenic peptides presented by classical MHC class I in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545835
更新日期:2016-04-01 00:00:00