Abstract:
:Ly49Q, a type II C-type lectin expressed on mouse plasmacytoid DC (pDC), contains a single carbohydrate recognition domain in its extracellular region and an ITIM in its cytoplasmic domain. We have identified the MHC class I molecule H-2K(b) as a Ly49Q ligand, confirming prior reports. Although H-2K(b) is expressed on essentially all hematopoietic cells, we found that only CpG-stimulated B cells were able to activate Ly49Q. This discovery correlated with our finding that although H-2K(b) forms clusters on CpG-activated B cells, it is diffusely expressed on resting B cells. Furthermore, CpG-stimulated, but not resting, B cells up-regulated co-stimulatory molecules on pDC. This finding was confirmed by the fact that binding by anti-Ly49Q mAb to Ly49Q led to pDC maturation in vitro. Our results suggest that clustered H-2K(b) on activated B cells act as ligands for Ly49Q and induce pDC maturation in vitro.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Toma-Hirano M,Namiki S,Shibata Y,Ishida K,Arase H,Miyatake S,Arai K,Kamogawa-Schifter Ydoi
10.1002/eji.200838363subject
Has Abstractpub_date
2009-05-01 00:00:00pages
1344-52issue
5eissn
0014-2980issn
1521-4141journal_volume
39pub_type
杂志文章abstract::Chemokine receptors are important for recruiting leukocytes to sites of infection and may contribute to immune cell activation. The present study investigated the role of the chemokine receptor CCR2 in polymicrobial septic peritonitis. The results showed that peritoneal production of the CCR2 ligands CCL2 and CCL12 in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425294
更新日期:2004-12-01 00:00:00
abstract::E-selectin is a cytokine-inducible membrane glycoprotein capable of mediating adhesion of leukocytes to endothelial cells. It is highly glycosylated, containing 11 sites for N-linked glycosylation. N-Glycosylation of E-selectin was analyzed by endoglycosidase treatment. Analysis of immunoprecipitated E-selectin from h...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250907
更新日期:1995-09-01 00:00:00
abstract::Polymorphonuclear neutrophils (PMN) modulate the adaptive immune response through interactions with immature dendritic cells (iDC) while spontaneous apoptotic neutrophils PMNapo (PMNapo) may have an inhibitory effect on DC functions. We investigate the effect exerted by PMNapo in DC maturation and the role of Mycobact...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636771
更新日期:2007-06-01 00:00:00
abstract::Vaccinia virus (VV)-encoded MHC class I Kd molecules which differ by a single amino acid change from glutamine (Kdw, wild type) to histidine (Kdm, mutant) at position 114 located in the floor of the peptide binding groove were compared in terms of peptide binding and cytotoxic T (Tc) cell recognition. Most anti-viral ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199904)29:04<1228::AID-IMM
更新日期:1999-04-01 00:00:00
abstract::5'-Nucleotidase/CD73 is a key enzyme in the regulation of purinergic signaling, hydrolyzing extracellular AMP to produce adenosine, which is critical in the blood vascular system and in immunosuppression. CD73 is expressed by both blood endothelial cells and lymphatic endothelial cells. Although the role of CD73 on bl...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201445400
更新日期:2015-02-01 00:00:00
abstract::The molecular mechanisms leading to anti-double-stranded (ds) DNA antibody production in systemic lupus erythematosus (SLE) are poorly understood. We describe here the immunoglobulin variable region genes of six human hybridomas secreting IgG anti-dsDNA antibodies derived from three SLE patients. The monoclonal IgG an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220709
更新日期:1992-07-01 00:00:00
abstract::Phenotypic classification of human CD8(+) T cells using three cell surface markers, CD27, CD28 and CD45RA, was recently suggested to be useful for identification of naive, memory and effector CD8(+) T cells. However, it still remains unclear whether such classification precisely reflects functional classification of C...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324478
更新日期:2004-04-01 00:00:00
abstract::A major feature of the immature immune system in the newborn is its inability to produce significant levels of immunoglobulins other than IgM in response to antigens. It has recently been demonstrated that interaction of the CD40 molecule on B cells with the CD40 ligand (CD40L) on activated T cells is pivotal for immu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240831
更新日期:1994-08-01 00:00:00
abstract::We recently reported that differentiation of CD8(+) T cells from the naïve to the effector state involves the upregulation of glucose-dependent metabolism. Glucose deprivation or inhibition of glycolysis by 2-deoxy-D-glucose (2-DG) selectively inhibited production of IFN-gamma but not of IL-2. To determine a more glob...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838289
更新日期:2008-09-01 00:00:00
abstract::Murine spleen and lymph node L3T4+ T cells were found to spontaneously produce high levels of interleukin 3 (IL3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in cultures containing 10% fetal calf serum (FCS) in the absence of other stimulation. The IL3 and GM-CSF activities in culture supernatants pe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180910
更新日期:1988-09-01 00:00:00
abstract::Similar idiotopes are detected on anti-allotypic antibodies synthesized by all rabbits immunized against the b6 allotype. The expression of similar idiotypes on anti-b6 antibodies has been found in rabbits of all VHa allotypic types thus far studies. For their expression, these idiotypes require the interaction betwee...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110606
更新日期:1981-06-01 00:00:00
abstract::PA28alpha/beta is a regulatory complex of the 20S proteasome which consists of two IFN-gamma inducible subunits. Both subunits, alpha and beta, contribute equally to the formation of hexa- or heptameric rings which can associate with the 20S proteasome. Previously, we have shown that overexpression of the PA28alpha su...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200012)30:12<3672::AID-IMMU3672>
更新日期:2000-12-01 00:00:00
abstract::Rapid IgE desensitization provides temporary tolerization for patients who have presented severe hypersensitivity reactions to food and drugs, protecting them from anaphylaxis, but the underlying mechanisms are still incompletely understood. Thus, here we develop an effective and reproducible in vitro model of rapid I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040810
更新日期:2011-04-01 00:00:00
abstract::The internalization and intracellular processing of monoclonal antibody to immunoglobulin mu heavy chain (Mamu) have been investigated in two human Burkitt lymphoma cell lines (Ramos and Raji), in a human B cell lymphoma and in normal human peripheral blood B cells. In addition to the degradation of 125I-labeled Mamu ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160314
更新日期:1986-03-01 00:00:00
abstract::The distribution of the murine receptor for interleukin (IL) 6 was examined on a variety of cells. Binding sites for IL 6 were found on many cell lines including fibroblasts and bone marrow-derived macrophages. The highest density of binding sites was found on B cell hybridomas and plasmacytomas, irrespective of their...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191121
更新日期:1989-11-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171227
更新日期:1987-12-01 00:00:00
abstract::Members of the innate lymphoid cell (ILC) family have been implicated in the development of thymic microenvironments and the recovery of this architecture after damage. However, a detailed characterization of this family in the thymus is lacking. To better understand the thymic ILC compartment, we have utilized multip...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201847511
更新日期:2018-09-01 00:00:00
abstract::The immunoglobulin lambda light chain system displays a limited diversity in inbred mice. Indeed, the lambda locus is organized in two recombination units: V lambda 2-V lambda x-J lambda 2-C lambda 2-psi J lambda 4-psi C lambda 4, which can produce either lambda 2(V2) or lambda 2(Vx) chains; and V lambda 1-J lambda 3-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240912
更新日期:1994-09-01 00:00:00
abstract::The involvement of the CD2 (T11) molecule, an alternative activation pathway for T lymphocytes, in the regulation of tumor necrosis factor (TNF)-alpha/TNF receptor system in human T lymphocytes has been investigated. It has been found that both TNF-alpha synthesis and secretion were induced after incubation of purifie...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830221219
更新日期:1992-12-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250636
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830161109
更新日期:1986-11-01 00:00:00
abstract::The expression of an X-linked defect in the CBA/N strain of mice has been found to result in a number of immune abnormalities. These include low responsiveness to antigens and a greatly reduced ability to respond to many of the common B cell mitogens. An in vitro manifestation of this condition is the virtual inabilit...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220419
更新日期:1992-04-01 00:00:00
abstract::Infection with parasitic worms (helminths) alters host immune responses and can inhibit pathogenic inflammation. Helminth infection promotes a strong Th2 and T regulatory response while suppressing Th1 and Th17 function. Th2 responses are largely dependent on transcriptional programs directed by Stat6-signaling. We ex...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201848072
更新日期:2020-10-16 00:00:00
abstract::Two major histocompatibility complex (MHC) class I-reactive T cell clones derived from H-2b mice, generated against the allogeneic Ld molecule, were found to recognize the H-2b class II mutant Abm12 molecule as well. In addition, these clones also recognize the class II A(s) molecule, and display a class II-dependent ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230823
更新日期:1993-08-01 00:00:00
abstract::The cloned human gamma 1 heavy chain gene (HIG1) was scarcely expressed in the stable transformants of a mouse fibroblast line, L cell or a T cell line, EL4, and no gamma 1 heavy chain was produced in these cells. Upon treatment with cycloheximide or other kinds of protein synthesis inhibitors, the transcription of HI...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170906
更新日期:1987-09-01 00:00:00
abstract::Between 10 and 20% of the peripheral gammadelta T cells express cytoplasmic TCR-beta proteins, but whether such TCR-beta chains can partake in alphabeta T-cell development has never been systematically investigated. Therefore, we reconstituted the T-cell compartment of CD3epsilon-deficient mice with Pax5-TCR-beta defi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838668
更新日期:2008-12-01 00:00:00
abstract::Because of their plasticity and central role in orchestrating immunity and tolerance, DCs can respond to pregnancy-specific signals, thus promoting the appropriate immune response in order to support pregnancy. Here, we show that pregnancy-specific glycoprotein (PSG1a), the major variant of PSG released into the circu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142140
更新日期:2012-06-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271107
更新日期:1997-11-01 00:00:00
abstract::To understand further the roles that negative regulatory signals may play in B cell immune responses, we compared three inhibitors of B cell proliferation: cross-linking CD19 with monoclonal antibody (mAb), signaling through Fc receptors by intact anti-mu mAb, and transforming growth factor-beta (TGF-beta). Each agent...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200516
更新日期:1990-05-01 00:00:00
abstract::Glycosylphosphatidylinositol-anchored (GPI)-Db molecules are defective in mediating cytotoxic T lymphocytes (CTL) lysis of transfected lymphoma cells, compared to their transmembrane (TM) counterpart. This defect is manifest when antigenic peptide must be processed and presented through the endogenous pathway. These s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260938
更新日期:1996-09-01 00:00:00