Abstract:
:In vivo, T cells expressing low-affinity TCR predominate in primary, but not in secondary responses, a process referred to as T cell affinity maturation. Using CD4 dependence as a measure of the avidity of the interaction between the allospecific TCR and the alloantigen, we show that a similar process occurs in mixed lymphocyte cultures in vitro. Moreover, in coculture experiments high-avidity (CD4-independent) T cell clones inhibited the TCR internalization of low-avidity (CD4-dependent) T cell clones, whereas low-avidity T cell clones had no such effect on high-avidity T cell clones. The extent of inhibition of TCR internalization was dependent on both the avidity of the competing clone and the number of competing cells. Thus, there was a cell dose- and avidity-dependent effect on TCR internalization, an early parameter in T cell activation. These results suggest that low- and high-avidity T cell clones compete for the availability of antigen-presenting cells and that this favors the selective outgrowth of high-avidity T cell clones.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
van Bergen J,Kooy Y,Koning Fdoi
10.1002/1521-4141(200102)31:2<646::aid-immu646>3.0keywords:
subject
Has Abstractpub_date
2001-02-01 00:00:00pages
646-52issue
2eissn
0014-2980issn
1521-4141pii
10.1002/1521-4141(200102)31:2<646::AID-IMMU646>3.0journal_volume
31pub_type
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