Abstract:
:The major histocompatibility complex (MHC)-encoded transporter associated with antigen processing (TAP) delivers cytosolic peptides to the lumen of the endoplasmic reticulum (ER) for presentation by MHC class I molecules. For the rat, it has been demonstrated that TAP polymorphism results in the selection of different sets of peptides, the nature of the C terminus being of particular importance. Here, we investigated whether TAP polymorphism in mice and humans has functional consequences for transport of peptide sets variable at the C-terminal residues. Using cell lines of H-2d, H-2k, and H-2dxk haplotype and a panel of human lymphoblastoid cell lines expressing eight different TAP alleles, we detected species-specific transport patterns, but no significant influence of TAP polymorphism on peptide selection. In addition, peptides with different core sequences were translocated to the same extent by different TAP. These results suggest that a major contribution of human TAP polymorphism to disease progression and autoimmunity is not very likely.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Obst R,Armandola EA,Nijenhuis M,Momburg F,Hämmerling GJdoi
10.1002/eji.1830250808subject
Has Abstractpub_date
1995-08-01 00:00:00pages
2170-6issue
8eissn
0014-2980issn
1521-4141journal_volume
25pub_type
杂志文章abstract::Gradual depletion of CD4+ T cells is a typical characteristic of pathogenic SIV infection. Intriguingly, we find a spontaneous CD4+ T-cell homeostasis in northern pig-tailed macaques (Macaca leonina) during SIVmac239 infection. ...
journal_title:European journal of immunology
pub_type: 信件
doi:10.1002/eji.201747284
更新日期:2018-02-01 00:00:00
abstract::Synthetic peptides encoding protective pathogen-derived epitopes represent--in principle--an ideal approach to T-cell vaccination. Empirically, however, these strategies have not been successful. In the current study, we profiled the early activation of CD8+ T cells by MHC class I-restricted peptide immunization to be...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939493
更新日期:2010-01-01 00:00:00
abstract::Co-infection of HCV with HIV has been associated with more rapid progression of HCV-related disease. HCV-specific T-cell immune responses, which are essential for disease control, are attenuated in co-infection with HIV. T-cell exhaustion has recently been implicated in the deficient control of chronic viral infection...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040340
更新日期:2010-09-01 00:00:00
abstract::The endoplasmic reticulum (ER) protein tapasin is essential for the loading of high-affinity peptides onto MHC class I molecules. It mediates peptide editing, i.e. the binding of peptides of successively higher affinity until class I molecules pass ER quality control and exit to the cell surface. The molecular mechani...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939342
更新日期:2010-01-01 00:00:00
abstract::Antigen-presenting macrophages (M phi) were derived from day 7 cultures of bone marrow stem cells using L cell conditioned medium. The adherent bone marrow-derived macrophages (BMM phi) were 100% esterase-positive, 95% positive for C3 receptors, 93% positive for Fc receptors, and 95% actively phagocytic. Indirect immu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830121202
更新日期:1982-12-01 00:00:00
abstract::Although the role of interleukin 2 (IL 2) in mature T lymphocyte function is well documented, its effect on hematopoietic progenitor cells is less well characterized. Here we have used recombinant retroviruses to transduce and express a cDNA clone encoding the p55 component of the human IL 2 receptor (h-p55), in a mur...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200317
更新日期:1990-03-01 00:00:00
abstract::X-linked agammaglobulinemia (XLA) is a humoral immunodeficiency disease in man, characterized by an arrest in B lymphocyte differentiation at the precursor B cell stage. The structure of expressed immunoglobulin (Ig) kappa light (L) chain rearrangements of nine B lymphoblastoid cell lines from one XLA patient was inve...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230306
更新日期:1993-03-01 00:00:00
abstract::Cyclopentenone prostaglandins (CP-PG), such as prostaglandin A1 (PGA1) or 15-deoxy-Delta(12,14)-prostaglandin J2 (PGJ2), induce apoptosis in different cell types. PGJ2 is also a potent activator of the peroxisome proliferator-activated receptor-gamma (PPARgamma). We investigated whether PPARgamma regulates CP-PG-induc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324053
更新日期:2004-01-01 00:00:00
abstract::A T3 complex-bearing subpopulation was characterized within an in vivo cycling T4-8- early thymocyte compartment which contains cells constitutively expressing interleukin 2 and transferrin receptors. We show differentiation in vitro of both mature subsets of thymocytes (T3+4+8- and T3+4-8+) from the above T4-8- compa...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160611
更新日期:1986-06-01 00:00:00
abstract::Dendritic cells isolated from sheep afferent lymph were examined for their ability to bind soluble protein and peptide antigens labeled with fluorescein both in in vitro assays and following intradermal injection of antigen in vivo. Analysis of dendritic cells by flow cytometry revealed weak direct binding of proteins...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830201102
更新日期:1990-11-01 00:00:00
abstract::We have fractionated human tonsillar B cells on the basis of CD5 expression and determined the nucleotide sequences of immunoglobin light chain variable (V) regions encoded by the single member of the V chi 4 gene family in both CD5+ and CD5- populations. The majority of cDNA from both CD5+ and CD5- B cells population...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230636
更新日期:1993-06-01 00:00:00
abstract::Thapsigargin (TG), which inhibits endoplasmic reticulum-dependent Ca(2 +)-ATPase and thereby increases cytosolic Ca(2 +), has been reported to cause apoptosis in T lymphocytes another cell types. In this study, we investigated the molecular mechanisms that are involved in the apoptosis induced by TG in T cell hybridom...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200106)31:6<1757::aid-immu1757>3
更新日期:2001-06-01 00:00:00
abstract::A recombinant Plasmodium vivax circumsporozoite (CS) protein (rPvCS-1) has been investigated as a possible malaria sporozoite vaccine candidate. Experiments were carried out to determine whether sporozoite-specific memory cells develop in Swiss Webster mice immunized with rPvCS-1. Challenge of rPvCS-1-immunized mice w...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171213
更新日期:1987-12-01 00:00:00
abstract::Phenotypic classification of human CD8(+) T cells using three cell surface markers, CD27, CD28 and CD45RA, was recently suggested to be useful for identification of naive, memory and effector CD8(+) T cells. However, it still remains unclear whether such classification precisely reflects functional classification of C...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324478
更新日期:2004-04-01 00:00:00
abstract::Dendritic cells (DC) are unique in their ability to present antigen to naive T cells, and therefore play a central role in the initiation of immune responses. Characterization of DC-specific genes may help to unravel the mechanism underlying their potent antigen presenting capacity. Here we describe the identification...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200012)30:12<3585::AID-IMMU3585>
更新日期:2000-12-01 00:00:00
abstract::At least two subsets of CD4+ T helper cell lymphocytes termed Th1 and Th2 exist in the mouse and probably in humans. They are characterized by the secretion of different lymphokines and by their functional behavior. Dysregulated expansion of one or the other subset may be one reason for the development of certain dise...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230805
更新日期:1993-08-01 00:00:00
abstract::The induction of cytotoxic T lymphocytes (CTL) specific for alloantigen in a primary mixed lymphocyte culture (MLC) was used as a model system to study the potential usefulness of monoclonal antibodies directed against the murine transferrin receptor in eliminating antigen-activated T cells. Two monoclonal antibodies ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150709
更新日期:1985-07-01 00:00:00
abstract::E-selectin is a cytokine-inducible membrane glycoprotein capable of mediating adhesion of leukocytes to endothelial cells. It is highly glycosylated, containing 11 sites for N-linked glycosylation. N-Glycosylation of E-selectin was analyzed by endoglycosidase treatment. Analysis of immunoprecipitated E-selectin from h...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250907
更新日期:1995-09-01 00:00:00
abstract::Many lymphocytes are produced in the intestinal mucosa, especially in the Peyer's patches. These newly formed lymphoid cells leave the gut wall, undergo further maturation and many reach the lamina propria of the intestinal mucosa where they function as effector and regulator cells of the intestinal immune response. H...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250336
更新日期:1995-03-01 00:00:00
abstract::Caspases are essential mediators of cytokine release and apoptosis. Additionally, caspase activity is required for the proliferation of naive T lymphocytes. It remained unclear how proliferating cells are able to cope with the pro-apoptotic activity especially of effector caspases-3 and -7. Possible reasons might incl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838211
更新日期:2008-07-01 00:00:00
abstract::Naive T cells do not proliferate but remain alive in vivo. In contrast, naive T cells rapidly die in an in vitro culture, suggesting that some factors that are present at the sites of naive T cell circulation in vivo but missing in the bovine serum-containing culture medium, are necessary for their survival. The prese...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425340
更新日期:2005-01-01 00:00:00
abstract::To detect possible differences in immunogenicity between tumors induced in T cell-deficient mice and phenotypically normal congenic mice, 16 sarcomas, 8 having developed in nude BALB/c mice and 8 having developed in congenic normal (nu/+) mice, were transplanted to normal BALB/c recipients and the rates of rejection o...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260827
更新日期:1996-08-01 00:00:00
abstract::The contribution of direct and indirect alloresponses by CD4(+) Th1 and Th2 cells in acute and chronic rejection of allogeneic transplants remains unclear. In the present study, we addressed this question using a transplant model in a single MHC class I-disparate donor-recipient mouse combination. BALB/c-dm2 (dm2) mut...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838980
更新日期:2009-11-01 00:00:00
abstract::We show that IL-13 in the presence of TNF-alpha effected an equal or greater antiviral activity against a dual-tropic HIV-1 (R5X4) in macrophages. A temporary or continued exposure of macrophages to both cytokines significantly decreased the infection and replication of R5X4 HIV-1(89.6) (median, 128-fold, n = 9, p = 0...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(200005)30:5<1340::AID-IMMU
更新日期:2000-05-01 00:00:00
abstract::Mutations of forkhead box p3 (FOXP3), the master gene for naturally occurring regulatory T cells (nTregs), are responsible for the impaired function of nTregs, resulting in an autoimmune disease known as the immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX) syndrome. The relevance of other periphe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040909
更新日期:2011-04-01 00:00:00
abstract::Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays an important role in regulating the local inflammatory immune response, but regulatory mechanisms of this cytokine have not been fully elucidated. Here, we demonstrate that IL-10 deficiency renders LPS treatment ineffective in regulating the expression...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141733
更新日期:2012-06-01 00:00:00
abstract::Nonspecific T cell factors produced by lectin-activated human peripheral blood lymphocytes (PBL) were used to restore the T-dependent B cell response to trinitrophenyl-polyacrylamide (TNP-PAA). Preincubation experiments with the particulate antigen TNP-PAA and/or a soluble TNP-protein conjugate show that a first speci...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160714
更新日期:1986-07-01 00:00:00
abstract::Anti-peroxidase antibody (Ab)-secreting hybrids have been produced by fusion of peroxidase (PO)-immunized mouse lymph node cells and immunoglobulin (Ig)-secreting P3-X63-Ag8 (X63) myeloma cells. Identification of Ab-secreting hybrids can be performed as early as day 5 after cell fusion by the hemolytic plaque assay. I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830090102
更新日期:1979-01-01 00:00:00
abstract::Pairs of monoclonal antibodies (mAb) defining epitopes T 11.1 and T 11.2 on the CD 2 molecule are mitogenic for purified human T cells in the presence of a submitogenic dose of 12-O-tetradecanoylphorbol 13-acetate (TPA). Anti-CD 28 mAb can substitute for the action of TPA in the anti-CD 2-induced proliferative respons...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180505
更新日期:1988-05-01 00:00:00
abstract::T-cell homeostasis preserves the numbers, the diversity and functional competence of different T-cell subsets that are required for adaptive immunity. Naïve CD4(+) T (TN ) cells are maintained in the periphery via the common γ-chain family cytokine IL-7 and weak antigenic signals. However, it is not clear how memory C...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201444701
更新日期:2014-10-01 00:00:00