Abstract:
:Developing T cells undergo distinct selection processes that determine the T cell receptor (TcR) repertoire. One of these processes is positive selection. Positive selection involves the differentiation to mature T cells of thymocytes bearing TcR capable of recognizing antigens in the context of self-major histocompatibility complex (MHC) molecules. To study the potential involvement of tyrosine phosphorylation in the mechanism of positive selection, we have analyzed the activities of the tyrosine kinases pp56lck and pp59fyn in thymocytes expressing a unique TcR specific for HY antigen+ H-2 Db. Thymocytes undergoing positive selection displayed higher kinase specific activities of pp56lck and pp59fyn than nonselecting thymocytes. Furthermore, these increases in kinase activities were found only in the CD4+CD8+ subpopulation of thymocytes, where the selection process is believed to occur. These data suggest that tyrosine phosphorylation is part of the intracellular signals involved in MHC class I-driven positive selection from CD4+CD8+ immature thymocytes to CD4-CD8+ mature cells.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Carrera AC,Baker C,Roberts TM,Pardoll DMdoi
10.1002/eji.1830220917keywords:
subject
Has Abstractpub_date
1992-09-01 00:00:00pages
2289-94issue
9eissn
0014-2980issn
1521-4141journal_volume
22pub_type
杂志文章abstract::Current models of lymphocyte traffic suggest that homing specificities of T cells to tissues such as skin are generated outside the thymus as a result of activation of naive T cells by antigen in lymph nodes. Virgin T cells are thought to home to high endothelial venules in lymph nodes, but are thought to be unable to...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250314
更新日期:1995-03-01 00:00:00
abstract::Previously we have reported on the production of two sets of human monoclonal antibodies reacting with mouse mammary tumor virus (MMTV) and human mammary tumor virus (HuMTV) cross-reacting antigens. B11 is a monoclonal IgG generated by the human-human hybridoma technique using axillary lymph node of breast cancer pati...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830181109
更新日期:1988-11-01 00:00:00
abstract::Graft-versus-host disease (GVHD) caused by donor T cells attacking recipient tissues is a major cause of morbidity and mortality following allogeneic hematopoietic stem cell transplantation (alloSCT). Studies have shown that effector memory T (T(EM) ) cells do not cause GVHD but are capable of immune functions post-tr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141678
更新日期:2011-09-01 00:00:00
abstract::The antigen receptor of the T lymphocytes is one of the most complex eukaryotic membrane structures studied to date. The T cell receptor (TcR) consists of two disulfide-linked glycoprotein chains (alpha/beta or gamma/delta) and is noncovalently associated with a group of small and invariable CD 3 proteins. Four CD 3 c...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180508
更新日期:1988-05-01 00:00:00
abstract::Mercury-induced autoimmune glomerulonephritis in the Brown-Norway (BN) rat is characterized by the successive appearance of linear and granular glomerular IgG deposits. Anti-laminin autoantibodies represent the major part of the anti-glomerular basement membrane (GBM) antibodies produced in this model. Fusions were pe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200114
更新日期:1990-01-01 00:00:00
abstract::The pathways of human anti-pig T cell xenorecognition have been investigated. Freshly isolated porcine alveolar lavage (AL) cells induced primary proliferative responses by human peripheral and cord blood mononuclear cells which were inhibited by anti-HLA-DR antibody (indirect xenorecognition). Following over-night cu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260630
更新日期:1996-06-01 00:00:00
abstract::The tight-skin (Tsk/+) mutant mouse, a putative murine model of scleroderma, is characterized primarily by the excessive deposition of collagen and other extracellular matrix molecules in the dermis, and also by a developmentally acquired defect in pulmonary architecture. Passive transfer experiments have suggested an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199809)28:09<2619::AID-IMM
更新日期:1998-09-01 00:00:00
abstract::Myeloid dendritic cells (DC) and macrophages evolve from a common precursor. However, factors controlling monocyte differentiation toward DC or macrophages are poorly defined. We report that the surface density of the GM-CSF receptor (GM-CSFR) alpha subunit in human peripheral blood monocytes varies among donors. Alth...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737395
更新日期:2008-03-01 00:00:00
abstract::Lipid antigens are presented to T cells by the non-polymorphic MHC class I-related CD1 molecules. Microsomal triglyceride transfer protein (MTP) is an endoplasmic reticulum (ER)-resident chaperone that has been shown to lipidate the group 2 CD1 molecule CD1d and thus to regulate its function. We now report that MTP al...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200738102
更新日期:2008-08-01 00:00:00
abstract::Mitogens such as pokeweed mitogen, Staphylococcus aureus Cowan I, concanavalin A and phytohemagglutinin and monoclonal anti-CD antibodies were examined for their capacity to induce IgE synthesis by peripheral blood leukocytes obtained from both atopic and nonatopic individuals. While lectins failed to induce IgE synth...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180322
更新日期:1988-03-01 00:00:00
abstract::Auto-reactive T cells present in healthy subjects remain in a state of unresponsiveness, but may trigger autoimmunity under various situations. Although myelin oligodendrocyte glycoprotein (MOG) is a potential target antigen in multiple sclerosis (MS), MOG-reactive T cell responses are present in the blood of both hea...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2001010)31:10<2942::aid-immu2942
更新日期:2001-10-01 00:00:00
abstract::PA28alpha/beta is a regulatory complex of the 20S proteasome which consists of two IFN-gamma inducible subunits. Both subunits, alpha and beta, contribute equally to the formation of hexa- or heptameric rings which can associate with the 20S proteasome. Previously, we have shown that overexpression of the PA28alpha su...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200012)30:12<3672::AID-IMMU3672>
更新日期:2000-12-01 00:00:00
abstract::Acute P. falciparum malaria is associated with loss of in vitro T cell responsiveness to antigenic stimulation, and with high plasma levels of soluble interleukin 2 receptor (IL 2R). In the present study peripheral T cells from acute P. falciparum malaria patients from a malaria-endemic area of Sudan were analyzed for...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210523
更新日期:1991-05-01 00:00:00
abstract::It is now recognized that Th17 cells constitute the third effector arm of Th cells, complementing the Th1 and Th2 lineages. Although secretion of IL-17 is a primary defining characteristic of Th17 cells, the expression profile of specific chemokine receptors, cell surface molecules and cytokines are also part of the T...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.200939540
更新日期:2009-06-01 00:00:00
abstract::A human IgE-producing myeloma has been cultivated in vitro as a continuous cell line (U-266) since 1968. Analysis of immunoglobulin production during early passages of the cell line demonstrated a high synthesis rate of monoclonal IgE. Analysis of late passages, cultivated after 1980, revealed a 3-6-fold lower rate of...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180611
更新日期:1988-06-01 00:00:00
abstract::Defined tumor-associated antigens (TAA) are attractive targets for anti-tumor immunotherapy. Here, we describe a novel genome-wide approach to identify multiple TAA from any given tumor. A panel of transplantable thymomas was established from an inbred p53-/- mouse strain. The resulting tumors were examined for gene e...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200104)31:4<1239::aid-immu1239>3
更新日期:2001-04-01 00:00:00
abstract::Riboflavin, also known as vitamin B2 , is converted by riboflavin kinase (RFK) into flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which are essential cofactors of dehydrogenases, reductases, and oxidases including the phagocytic NADPH oxidase 2 (Nox2). Riboflavin deficiency is common in young adul...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201343940
更新日期:2014-03-01 00:00:00
abstract::The major role of cells of the dendritic family in immunity and tolerance has been amply documented. Since their discovery in 1973, these cells have gained increasing interest from immunologists, as they are able to detect infectious agents, migrate to secondary lymphoid tissue, and prime naive T lymphocytes, thereby ...
journal_title:European journal of immunology
pub_type: 杂志文章,评审
doi:10.1002/eji.201242480
更新日期:2012-10-01 00:00:00
abstract::An immunoglobulin epsilon-chain fragment was synthesized in E. coli by cloning and expression of the gene coding for the second, third and fourth constant domains of the human IgE heavy chain. The bacterial CH2-4 polypeptide product was assembled by oxidation into a covalently linked dimeric epsilon-chain molecule pre...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150920
更新日期:1985-09-01 00:00:00
abstract::The murine T cell response to heterologous insulins provides a good model system for studying the mechanism of immune response (Ir)-gene function, since insulin is a small, chemically well-defined molecule. H-2b mice respond predominantly to A chain loop determinants of beef insulin, presented by the I-A epitope Ia. W...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830141208
更新日期:1984-12-01 00:00:00
abstract::CD4+ helper T lymphocytes and CD8+ killer T lymphocytes are both generated in the thymus from common precursor cells expressing CD4 and CD8. The development of immature CD4 CD8+ thymocytes into mature 'single-positive' T cells requires T cell antigen-receptor (TCR)-mediated positive selection signals. Although it is k...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830271231
更新日期:1997-12-01 00:00:00
abstract::Previous studies suggested that trinitrophenyl (TNP)-modified syngeneic red cells induced humoral autoimmune response in mice with defective T cell function but not in normal mice. The ability of modified self antigen to induce autoimmune response in immunodeficient mice was further explored using the delayed-type hyp...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830121112
更新日期:1982-11-01 00:00:00
abstract::This study shows that both recombinant human interleukin (rhIL)3 and rhIL4 induced proliferation in bone marrow (BM) cells of myelogenous leukemia patients in a manner similar to that reported using normal BM cells. However, we additionally found that these cytokines also influenced expression of other cytokines. Name...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830191108
更新日期:1989-11-01 00:00:00
abstract::Activated human T cells via the CD2 or the CD3 pathways exhibited a higher capacity than resting T lymphocytes to incorporate and metabolize [3H]pafacether (paf) at 37 degrees C. Resting T lymphocytes lacked specific binding capacity for paf, yet high-affinity paf receptors (paf-R) were induced on CD3- or CD2-dependen...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220604
更新日期:1992-06-01 00:00:00
abstract::We analyzed the functional outcome of the interaction between tumor-associated macrophages (TAMs) and natural killer (NK) cells. TAMs from ascites of ovarian cancer patients displayed an alternatively activated functional phenotype (M2) characterized by a remarkably high frequency and surface density of membrane-bound...
journal_title:European journal of immunology
pub_type: 临床试验,杂志文章
doi:10.1002/eji.201344130
更新日期:2014-06-01 00:00:00
abstract::Murine cytolytic T cell lines have been analyzed for the expression of two surface glycoproteins called T145 and T130. T145, known to be expressed by activated cytolytic T cells, is also expressed by such lines, but T130, which has been described by a universal T cell marker, is not. Our results suggest a structural r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830101111
更新日期:1980-11-01 00:00:00
abstract::Syntaxin 11 (STX11) controls vesicular trafficking and is a key player in exocytosis. Since Stx11 mutations are causally associated with a familial hemophagocytic lymphohistio-cytosis, we wanted to clarify whether STX11 is functionally important for key immune cell populations. This was studied in primary cells obtain...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142343
更新日期:2013-01-01 00:00:00
abstract::Upon antigen-specific or allogeneic activation, T cells sharply increase their metabolic activity to cope with augmented needs for proliferation and effector functions. Therefore, enzymes involved in energy metabolism constitute attractive targets to modulate the activity of pathogenic effector T cells in the setting ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201546152
更新日期:2016-09-01 00:00:00
abstract::Carbon monoxide (CO) treatment improves pathogenic outcome of autoimmune diseases by promoting tolerance. However, the mechanism behind this protective tolerance is not yet defined. Here, we show in a transgenic mouse model for autoimmune diabetes that ex vivo gaseous CO (gCO)-treated DCs loaded with pancreatic β-cell...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242684
更新日期:2013-01-01 00:00:00
abstract::Tissue distribution and expression on mitogen and virally stimulated lymphocytes render the ACT35 molecule a human lymphocyte activation antigen which as yet could not be clustered. Expression cloning of the ACT35 antigen from a pCDM8 library of the HUT-102 cell line revealed strong homology of the cDNA and its encode...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240329
更新日期:1994-03-01 00:00:00