Abstract:
:This study shows that both recombinant human interleukin (rhIL)3 and rhIL4 induced proliferation in bone marrow (BM) cells of myelogenous leukemia patients in a manner similar to that reported using normal BM cells. However, we additionally found that these cytokines also influenced expression of other cytokines. Namely, using a reproducible dot blot hybridization technique we observed on the one hand that BM cells were capable of constitutively expressing low levels of cytokine mRNA coding for IL3, IL4, granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte (G)-CSF and IL 1 beta, and on the other hand that in normal peripheral blood mononuclear cells rhIL4 inhibited mRNA expression coding for GM-CSF, G-CSF, IL3 and IL 1 beta, while IL4 mRNA and 28S rRNA was not affected. In contrast, rhIL3 marginally enhanced mRNA coding for IL3, GM-CSF, G-CSF and IL 1 beta and counteracted the inhibitory effect of IL4. In long-term cultures rhIL3 and rhIL4 had no significant effect on spontaneous cytokine gene expression of myelogenous leukemia-derived peripheral blood or BM cells, but made these cells more sensitive for subsequent stimulation with different polyclonal stimuli. Thus, IL3 and IL4 already modulate cytokine gene expression during the initiation of cell culture and differentiate BM cells into populations of cells which are capable of responding with an enhanced cytokine gene expression after polyclonal stimulation.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Brantschen S,Gauchat JF,De Weck AL,Stadler BMdoi
10.1002/eji.1830191108subject
Has Abstractpub_date
1989-11-01 00:00:00pages
2017-23issue
11eissn
0014-2980issn
1521-4141journal_volume
19pub_type
杂志文章abstract::From several years, the anticancer effects of Vγ9 T lymphocytes make these cells good candidates for cancer immunotherapies. However, the proved efficacy of γδ Τ cell-based cancer immunotherapies in some clinical trials was minimized due to the inherent toxicity of IL-2, which is essential for the combination therapy ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747093
更新日期:2017-12-01 00:00:00
abstract::Peritoneal macrophages from C57BL/6 mice process antigens from bacteria or coated on polystyrene beads for presentation by major histocompatibility complex (MHC) class I molecules. To investigate this antigen processing pathway, peritoneal macrophages from homozygous TAP1-/- mice, which lack the transporter associated...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261135
更新日期:1996-11-01 00:00:00
abstract::Cellular metabolism is dynamically regulated in NK cells and strongly influences their responses. Metabolic dysfunction is linked to defective NK cell responses in diseases such as obesity and cancer. The transcription factors, sterol regulatory element binding protein (SREBP) and cMyc, are crucial for controlling NK ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048784
更新日期:2020-09-18 00:00:00
abstract::Myeloid leukocytes form actin-based plasma membrane protrusions, called podosomes, that are implicated in myeloid cell recruitment into tissues and cell migration within the interstitium. In this study, we show that tyrosine kinases of the Abl family are present in podosomes formed by murine and human macrophages. Sil...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142270
更新日期:2012-10-01 00:00:00
abstract::The pathogenesis of the decline of CD4 lymphocyte counts accompanying the typical course of HIV-1 infection is not completely defined and might be related to a differential susceptibility of naive and memory cells to HIV-1 exposure. Here, we examined the effects induced by heat-inactivated HIV-1 virions on these lymph...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199906)29:06<1879::AID-IMM
更新日期:1999-06-01 00:00:00
abstract::Gradual depletion of CD4+ T cells is a typical characteristic of pathogenic SIV infection. Intriguingly, we find a spontaneous CD4+ T-cell homeostasis in northern pig-tailed macaques (Macaca leonina) during SIVmac239 infection. ...
journal_title:European journal of immunology
pub_type: 信件
doi:10.1002/eji.201747284
更新日期:2018-02-01 00:00:00
abstract::Leishmania-induced macrophage (Mphi) dysfunctions have been correlated with altered signaling events. Recent findings from our laboratory suggest that modulation of host protein tyrosine phosphatase (PTP) following Leishmania infection could lead to these Mphi defects. To address this issue, Mphi PTP activity and IFN-...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199911)29:11<3737::AID-IMM
更新日期:1999-11-01 00:00:00
abstract::The F1 progeny of autoimmune NZB and normal SWR mice uniformly develop severe and accelerated lupus nephritis. The (SWR x NZB)F1 mice produce an oligoclonally expanded population of nephritogenic anti-DNA autoantibodies that share a recurrent cross-reactive idiotype (Id564), use highly homologous VH genes and surprisi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220302
更新日期:1992-03-01 00:00:00
abstract::Tissue macrophages and their precursors-the blood monocytes-respond rapidly to a bacterial infection with the release of inflammatory mediators. These mediators are involved in the recruitment of phagocytic cells, principally neutrophils, from the blood to the site of infection. To initiate this process macrophages an...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250545
更新日期:1995-05-01 00:00:00
abstract::The conditions under which tumor necrosis factor-alpha (TNF) induces apoptosis in primary microvascular endothelial cells (MVEC) were investigated. In the absence of sensitizing agents, TNF induced apoptosis after 3 days of incubation in confluent MVEC. In contrast, upon addition of the transcriptional inhibitor actin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199811)28:11<3577::AID-IMM
更新日期:1998-11-01 00:00:00
abstract::Autoimmune diseases including multiple sclerosis (MS) are the result of an imbalanced immune tolerance network. Dendritic cells (DCs) are key players in both initiating immunity (immunogenic DCs) and regulating immune responses (tolerogenic DCs = tolDCs) and are potential targets for the treatment of MS. While the imm...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201242402
更新日期:2012-03-01 00:00:00
abstract::CD72 has been reported to regulate BCR-mediated signals both positively and negatively. SHP-1 and Grb2 bind, respectively, to ITIM1 and ITIM2 of CD72. We generated transformed B cell lines with an immature phenotype following J2 virus infection of splenocytes from CD72(-/-) and wild-type (Wt) mice. The transformed lin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425775
更新日期:2005-05-01 00:00:00
abstract::The proliferative state of functionally defined populations of size-separated pre-B cells was investigated. The results presented here support the hypothesis that pre-B cells are in the G2 phase when stable membrane IgM is first expressed. Small resting precursor cells significantly adhere to nylon wool columns, unlik...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830120406
更新日期:1982-04-01 00:00:00
abstract::Current concepts on the pathogenesis of chronic asthma emphasize the role of several inflammatory cell populations and their respective mediators that interact in a complex network. However, beside inflammatory cells, lymphocytes are also present in asthmatic airways. Although little is known about their involvement i...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230404
更新日期:1993-04-01 00:00:00
abstract::IL-12 is a potent inducer of IFN-gamma production and drives the development of Th1 cells. Human polarized Th2 cells do not express the signaling beta2-subunit of the IL-12R and, therefore, do not signal in response to IL-12. The question was raised as to what extent the loss of the IL-12Rbeta2 chain in Th2 cells has ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200104)31:4<1055::aid-immu1055>3
更新日期:2001-04-01 00:00:00
abstract::Activated monocyte-macrophages have been implicated in tumor angiogenesis via their capacity to produce many potent angiogenic factors. However, the mechanisms leading to production of these angiogenic factors in macrophages remain to be elucidated. In this study, we demonstrated by use of a mouse Matrigel implantatio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200424957
更新日期:2004-08-01 00:00:00
abstract::The mechanism of synergistic complement-mediated lysis of rat red cells was investigated using rat monoclonal antibodies against class I RT1Aa antigens. The increased lytic activity when using two antibodies simultaneously is due to the increase in the number of activated C1 molecules on the cell surface and this resu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130806
更新日期:1983-08-01 00:00:00
abstract::A major contributing factor to the final magnitude and breadth of CD8(+) T-cell responses to complex antigens is immunodomination, where CD8(+) T cells recognizing their cognate ligand inhibit the proliferation of other CD8(+) T cells engaged with the same APC. In this study, we examined how the half-life of cell s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142010
更新日期:2012-01-01 00:00:00
abstract::The exact identification of B cell subsets is instrumental to understand their dynamics under physiological and pathological conditions. Human memory B cells are currently identified according to the expression of CD27, which is absent on naive B cells. We report here that the ATP-binding cassette (ABC)B1 transporter ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200535364
更新日期:2005-12-01 00:00:00
abstract::Blockade of the immune response, caused by a high dose of Salmonella typhi Vi antigen (200 microgram i.v.) and cyclophosphamide (CY)-induced tolerance to Vi antigen, were analyzed. The results of the study show that blockade of the immune response cannot be attributed to masking of the response resulting from neutrali...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830100812
更新日期:1980-08-01 00:00:00
abstract::Naive T cells do not proliferate but remain alive in vivo. In contrast, naive T cells rapidly die in an in vitro culture, suggesting that some factors that are present at the sites of naive T cell circulation in vivo but missing in the bovine serum-containing culture medium, are necessary for their survival. The prese...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425340
更新日期:2005-01-01 00:00:00
abstract::T-follicular helper (TFH) cells represent the subpopulation of CD4(+) T cells that provides help for antigen-specific B cells in the GC response. They are generated from naïve T cells during an immune response and are imprinted by their master transcription factor Bcl-6. It has been a long-standing question if TFH ce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242540
更新日期:2012-08-01 00:00:00
abstract::Chloride intracellular channel (CLIC) 4 has diverse functions in membrane trafficking, apoptosis, angiogenesis and cell differentiation. CLIC4 is abundantly expressed in macrophages, but its role in innate immune functions is unclear. Here, we show that primary murine macrophages express increased amounts of CLIC4 aft...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201041266
更新日期:2011-05-01 00:00:00
abstract::In this report we investigated the production and role of interleukin (IL)2 and IL4 in the generation of antigen-specific cytotoxic T cells (CTL). We used as our model the ultraviolet-light-induced epithelial tumor 1591, a highly immunogenic regressor tumor which evokes a strong cell-mediated immune response leading t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210813
更新日期:1991-08-01 00:00:00
abstract::Proliferation of BCL1 cells, generally used for the measurement of murine interleukin (IL)5, is shown to be strongly suppressed by interferon (IFN)-gamma. This effect can be abrogated by the addition of anti-IFN-gamma antibodies. Thus, the IL5 activity of culture supernatants derived from T cells simultaneously produc...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830190726
更新日期:1989-07-01 00:00:00
abstract::We recently reported that parenteral injection of malaria palmitoyl-tailed peptides without adjuvant efficiently induces B, Th and CTL responses. We now show that intranasal (IN) or sub-lingual (SL) delivery of such lipopeptides induces strong systemic immune responses, as demonstrated by specific Th cell responses fr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200208)32:8<2274::AID-IMMU2274>3
更新日期:2002-08-01 00:00:00
abstract::Cyclosporin A (CSA), an immunosuppressive agent used in organ transplantation and to treat some autoimmune diseases, blocks the Ca2+-dependent steps involved in T cell receptor triggering leading to interleukin (IL)-2 production. Considering that the early steps of T cell activation are insensitive to CSA, we asked wh...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260712
更新日期:1996-07-01 00:00:00
abstract::The lymph node cells (LNC) activated in vivo by liver regeneration following partial hepatectomy of mice (primed lymph node cells) respond to regenerating liver cells in vitro with typical secondary immune response characteristics (Miyahara, S. et al., Eur. J. Immunol. 1983. 13: 878). These LNC activated in vivo suppr...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830131105
更新日期:1983-11-01 00:00:00
abstract::E-selectin is an endothelium-specific inducible adhesion molecule which binds several inflammatory cell types, including neutrophils, monocytes, natural killer cells and a subset of memory T cells. E-selectin is important in the initial rolling interaction of these cells on inflamed endothelium. The transient kinetics...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830261221
更新日期:1996-12-01 00:00:00
abstract::T helper cell type 2 (Th2) cells when triggered by antibodies to CD3 acquire the capacity to stimulate the polyclonal proliferation of syngeneic, resting B cells. Here, we tested the ability of various monoclonal antibodies (mAb) to block the B cell proliferation-inducing potential of such activated Th2 cells. We demo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220246
更新日期:1992-02-01 00:00:00