Abstract:
:Cyclosporin A (CSA), an immunosuppressive agent used in organ transplantation and to treat some autoimmune diseases, blocks the Ca2+-dependent steps involved in T cell receptor triggering leading to interleukin (IL)-2 production. Considering that the early steps of T cell activation are insensitive to CSA, we asked whether the initial activation achieved in presence of this immunosuppressor could affect the capacity of the T cell to respond to a mitogenic restimulation. We found that T cells activated by concanavalin A (ConA) for 48 h in the presence of CSA retain the capacity to proliferate in response to ConA once the immunosuppressor is removed. These cells are able to transcribe anew the IL-2 gene, without the requirement of new protein synthesis, and to up-regulate the alpha chain of the IL-2 receptor. Furthermore, we present the first direct evidence that the nuclear factor AP-1 is present in the nucleus of the T cells primed for 48 h in presence of CSA and that withdrawal of the immunosuppressor leads to the translocation of NFATp from the cytoplasm to the nucleus.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Bemer V,Truffa-Bachi Pdoi
10.1002/eji.1830260712subject
Has Abstractpub_date
1996-07-01 00:00:00pages
1481-8issue
7eissn
0014-2980issn
1521-4141journal_volume
26pub_type
杂志文章abstract::Lymphoid follicles of the ileal Peyer's patch (PP) of young sheep function as the major source of B cells and a site of immunoglobulin (Ig) receptor diversification. However, extensive cell death in culture has restricted investigations of ileal PP follicular (iPf)B cell biology. We investigated the possibility that s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240220
更新日期:1994-02-01 00:00:00
abstract::Inflammatory bowel diseases (IBD) are chronic conditions that result from uncontrolled intestinal inflammation. Pathogenic Th17 cells, characterized by production of IL-17A in the absence of IL-10, are thought to contribute to this inflammation, but in humans, antibody-mediated blockade of IL-17A is an ineffective IBD...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201948435
更新日期:2020-09-01 00:00:00
abstract::The murine intestinal epithelium contains a heterogeneous population of intraepithelial leukocytes (IEL) most of which are granulated, Thy-1-CD5-CD8+. In order to assess the lineage relationship of this subgroup of IEL to peripheral T cells, we examined IEL in mice with the severe combined immunodeficiency (scid/scid)...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200327
更新日期:1990-03-01 00:00:00
abstract::In this study, we examined which cell population contributes to IL-5 production by Peyer's patch (PP) cells. Thy1.2(-) fraction of PP cells, but not those of splenocytes, secreted IL-5 in response to IL-2. We found that CD3epsilon(-)IL-2Ralpha(+) cells purified from the Thy1.2(-)B220(-) fraction of PP cells secreted I...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324696
更新日期:2004-07-01 00:00:00
abstract::The active role of chemokines in the central nervous system (CNS) during the pathogenesis of experimental autoimmune encephalomyelitis (EAE) has been clearly established. In this study the expression pattern of several chemokines and cytokines was elucidated using reverse transcription-PCR and immunohistochemistry in ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200007)30:7<1911::AID-IMMU1911>3
更新日期:2000-07-01 00:00:00
abstract::T-cell activation and the subsequent transformation of activated T cells into T-cell blasts require profound changes in cell volume. However, the impact of cell volume regulation for T-cell immunology has not been characterized. Here we studied the role of the cell-volume regulating osmolyte transporter Taut for T-cel...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141690
更新日期:2012-04-01 00:00:00
abstract::In experimental autoimmune encephalomyelitis (EAE), intravenous transfer of activated CD4(+) myelin-specific T cells is sufficient to induce disease. Transferred T cells access the CNS parenchyma by trafficking across the blood brain barrier (BBB) vascular endothelium into the perivascular space, and then across the g...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425864
更新日期:2005-04-01 00:00:00
abstract::High frequencies of CD5+TcR alpha/beta- T cells were induced in the peritoneal cavity of rats immunized with syngeneic W439 lymphoma cells. These TcR alpha/beta- cells expressed TcR delta mRNA as analyzed by the polymerase chain reaction technique. The delta + (TcR gamma/delta +) T cells were of the CD2+, CD3+, CD4-, ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830211122
更新日期:1991-11-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231117
更新日期:1993-11-01 00:00:00
abstract::The proliferative state of functionally defined populations of size-separated pre-B cells was investigated. The results presented here support the hypothesis that pre-B cells are in the G2 phase when stable membrane IgM is first expressed. Small resting precursor cells significantly adhere to nylon wool columns, unlik...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830120406
更新日期:1982-04-01 00:00:00
abstract::A gene-encoding ligand for deletion of T cells bearing TcRV beta 6 and V beta 8.1 cosegregates a new mammary tumor provirus locus, Mtv-50 in NC mice. The sequence of the open reading frame (ORF) in the 3' long terminal repeat (LTR) of Mtv-50 was strikingly similar to those of Mtv-7, Mtv-43 and exogenous mouse mammary ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240235
更新日期:1994-02-01 00:00:00
abstract::The V beta 8.3-specific superantigenic lectin Urtica dioica agglutinin (UDA) was used to delete the V beta 8.3+ T cells in MRL lpr/lpr mice. In contrast to the systemic lupus erythematosus-like pathology which progresses with age in the phosphate-buffered saline-injected MRL lpr/lpr controls, UDA-treated animals did n...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260807
更新日期:1996-08-01 00:00:00
abstract::Endogenous superantigens (SAg) presented by MHC class II IA molecules induce slow-evolving negative selection of alpha beta T cells. The role of both B and gamma delta T cells on the regulation of these SAg-specific alpha beta T cell responses was addressed in IA(b+)IE(b-) C57BL/6 mice bearing genetically induced B ce...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200109)31:9<2811::aid-immu2811>3
更新日期:2001-09-01 00:00:00
abstract::The expression of HLA class II genes is under the control of a transcriptional activator, CIITA, encoded by the AIR-1 locus. Here we show that CIITA inhibits HIV-1 LTR transactivation mediated by Tat. The inhibition occurred when CIITA and Tat were transiently expressed in cells after transfection and, most importantl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(2002010)32:10<2783::AID-IMMU2783
更新日期:2002-10-01 00:00:00
abstract::Cell suspensions enriched for N cells, the effector cell for human natural cell-mediated cytotoxicity, were observed to react strongly with a rabbit antiserum directed against an antigen associated with B cells. Treatment of the effector suspension with the antiserum resulted in inhibition of natural cell-mediated cyt...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830071016
更新日期:1977-10-01 00:00:00
abstract::IL-5 preferentially activates B1 cells to produce natural antibodies cross-reactive to self antigens. To determine the role of IL-5 in antibody-mediated autoimmune disease, we generated systemic lupus erythematosus (SLE)-prone (NZB x NZW)F1 mice congenic for IL-5 transgene (TG-F1). The transgene unexpectedly reduced t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200425267
更新日期:2004-10-01 00:00:00
abstract::In this study, after immunization with NK cells from a KIR2DS5(+) donor and screening on cell transfectants expressing different members of the killer immunoglobulin-like receptor (KIR) family, we generated a mAb, DF200, reacting with several KIR2D receptors including KIR2DL1/L2/L3, KIR2DS1/S2 and KIR2DS5. By the anal...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838434
更新日期:2008-08-01 00:00:00
abstract::The influence of Th2 cytokines in tuberculosis has been a matter of dispute. Here we report that IL-4 has a profound regulatory effect on the infection of BALB/c mice with Mycobacterium tuberculosis. Depletion of IL-4 with a neutralizing mAb caused only evanescent reduction of lung infection, but when combined with i....
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200636764
更新日期:2007-03-01 00:00:00
abstract::The existence of a functional receptor for secretory component (SC) on the eosinophil membrane might explain the preferential degranulation induced by secretory IgA (sIgA) when compared to serum IgA. Indeed, flow cytometry analysis revealed that purified human SC could bind to a subpopulation (4-59%) of blood eosinoph...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830250121
更新日期:1995-01-01 00:00:00
abstract::Murine cytolytic T cell lines have been analyzed for the expression of two surface glycoproteins called T145 and T130. T145, known to be expressed by activated cytolytic T cells, is also expressed by such lines, but T130, which has been described by a universal T cell marker, is not. Our results suggest a structural r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830101111
更新日期:1980-11-01 00:00:00
abstract::We examined the ability of oral or parenteral immunization with immune stimulating complexes containing ovalbumin (ISCOMS-OVA) to prime T cell proliferative and cytokine responses. A single subcutaneous immunization with ISCOMS-OVA primed potent antigen-specific proliferative responses in the draining popliteal lymph ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251019
更新日期:1995-10-01 00:00:00
abstract::Antigen-presenting macrophages (M phi) were derived from day 7 cultures of bone marrow stem cells using L cell conditioned medium. The adherent bone marrow-derived macrophages (BMM phi) were 100% esterase-positive, 95% positive for C3 receptors, 93% positive for Fc receptors, and 95% actively phagocytic. Indirect immu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830121202
更新日期:1982-12-01 00:00:00
abstract::Longitudinal studies on the kinetics of viral antigen specific CD8 T cell responses have led to a model whereby a relatively small subset of the primary effector CD8 T cells expanding after the first week of acute viral infection initiate a program of cell survival and differentiation into long lived memory T cells. T...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.200535516
更新日期:2005-11-01 00:00:00
abstract::Age-associated thymic involution results in a diminished capacity to regenerate T cell populations, although the magnitude of this effect is unknown. In this report, thymic function was studied in aged vs. young adult mice after lethal irradiation and administration of T cell-depleted bone marrow (BM) from young mice....
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199806)28:06<1886::AID-IMM
更新日期:1998-06-01 00:00:00
abstract::The proteins p40 (Mr = 40 000), p32 (Mr = 32 000), p28 (Mr = 28 000), p20 (Mr = 20 000) and p10 (Mr = 10 000) are described which occur in noncovalent association with the polymorphic alpha, beta heterodimer of class II antigens. They were investigated with respect to their molecular characteristics and their mutual s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140606
更新日期:1984-06-01 00:00:00
abstract::Activated leukocyte cell adhesion molecule (ALCAM; CD166) is a member of the immunoglobulin gene superfamily (IgSF) which is expressed by activated leukocytes and thymic epithelial cells and is a ligand for the lymphocyte antigen CD6. Herein, we report on the isolation and characterization of cDNA clones encoding mous...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270625
更新日期:1997-06-01 00:00:00
abstract::We show that latently gammaherpesvirus-infected B cells are present in the thymus. This could result in a functional T-cell tolerance against certain viral epitopes. It is conceivable that also antigens from other viruses or pathogens may be conveyed to the thymus for their immune evasion. ...
journal_title:European journal of immunology
pub_type: 信件
doi:10.1002/eji.201847886
更新日期:2019-02-01 00:00:00
abstract::The cornea is an immune privileged tissue. Since arginase has been found to modulate T-cell function by depleting arginine, we investigated the expression of arginase in the cornea and its possible role in immune privilege using a murine transplant model. We found that both the endothelium and epithelium of murine cor...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141683
更新日期:2011-10-01 00:00:00
abstract::The F1 progeny of autoimmune NZB and normal SWR mice uniformly develop severe and accelerated lupus nephritis. The (SWR x NZB)F1 mice produce an oligoclonally expanded population of nephritogenic anti-DNA autoantibodies that share a recurrent cross-reactive idiotype (Id564), use highly homologous VH genes and surprisi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220302
更新日期:1992-03-01 00:00:00
abstract::Cycling lymphocytes may express the enzyme telomerase which is involved in maintenance of telomere length and cell proliferation potential. In CD8(+) T cells freshly isolated from peripheral blood, we found that in vivo cycling cells expressed HLA-DR. Furthermore, CD28-positive cells are known to have longer telomeres...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200102)31:2<459::aid-immu459>3.0
更新日期:2001-02-01 00:00:00