Abstract:
:Incorporation of an 11 amino acid region of the HIV TAT protein transduction domain (TAT PTD) into proteins facilitates rapid, efficient entry into cells. We previously showed that rTAT-PTD-OVA-transduced dendritic cells (DC) stimulated antigen (Ag)-specific CTL and Th cells, and vaccinated against OVA-expressing tumors. Here we studied B16 melanoma in C57BL/6 mice, using murine tyrosinase-related protein 2 (Trp2) as a candidate tumor Ag. We produced a 472-amino acid N-terminal fragment of Trp2 protein (rTrp2Delta) with or without PTD. Although PTD-deficient rTrp2Delta was ineffective, mice given rTAT-PTD-Trp2Delta-transduced DC were efficiently primed for Trp2(180-188) peptide-specific and B16-reactive CTL. In 58% of such mice, growth of melanomas was prevented. Trp2(180-188) peptide-pulsed DC protected 35% of recipients, and irradiated GM-CSF-producing B16 cells protected 75%. rTAT-PTD-Trp2Delta-transduced DC induced a more vigorous memory response to B16 rechallenge than the other regimens, and protected 30% of recipients from progressive tumor development in treatment studies. In this setting, Trp2 peptide-treated DC protected 20% and irradiated GM-CSF-producing cells protected 0%. Both tumor prevention and tumor treatment were CD8(+) T cell dependent. Vaccination with rTAT-PTD-Trp2Delta-transduced DC induced a robust CTL response and durable anti-melanoma immunity. This approach should be clinically applicable, and offers theoretical and practical advantages to those that are in current use.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Shibagaki N,Udey MCdoi
10.1002/eji.200323709keywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
850-60issue
4eissn
0014-2980issn
1521-4141journal_volume
33pub_type
杂志文章abstract::High frequencies of CD5+TcR alpha/beta- T cells were induced in the peritoneal cavity of rats immunized with syngeneic W439 lymphoma cells. These TcR alpha/beta- cells expressed TcR delta mRNA as analyzed by the polymerase chain reaction technique. The delta + (TcR gamma/delta +) T cells were of the CD2+, CD3+, CD4-, ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830211122
更新日期:1991-11-01 00:00:00
abstract::A series of rat monoclonal antibodies (mAb) directed against the Thy-1 molecule have been evaluated for their ability to stimulate immature (CD4-8-) murine thymocytes. CD4-8- thymocytes could be induced to proliferate by several anti-Thy-1 mAb provided that exogenous interleukin 1 or interleukin 2 was provided. Appare...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171127
更新日期:1987-11-01 00:00:00
abstract::A monoclonal antibody (mAb) directed to a human leukocyte 55-kDa cell surface molecule with identical cellular distribution and biochemical properties to the CD4 was able to inhibit T cell proliferation induced either in a mixed lymphocyte culture or by activation with mAb anti-CD3, anti-CD2 or phytohemagglutinin. The...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170205
更新日期:1987-02-01 00:00:00
abstract::We have analyzed the inducibility of protein kinase C (PKC)-dependent expression of CD 69 molecules in T cell receptor (TCR) transgenic thymocytes developing in the presence or absence of selecting, class I major histocompatibility complex (MHC) molecules. Small CD4+8+ thymocytes developing in the absence of selecting...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230326
更新日期:1993-03-01 00:00:00
abstract::HIV controllers are a valuable source for the identification of HIV-neutralizing antibodies, as chronic infection over decades allows extensive affinity maturation of antibodies for improved Ag recognition. We analyzed a small cohort of elite controllers (ECs) for HIV-neutralizing antibodies using a panel of standardi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201242974
更新日期:2013-02-01 00:00:00
abstract::We show that IL-13 in the presence of TNF-alpha effected an equal or greater antiviral activity against a dual-tropic HIV-1 (R5X4) in macrophages. A temporary or continued exposure of macrophages to both cytokines significantly decreased the infection and replication of R5X4 HIV-1(89.6) (median, 128-fold, n = 9, p = 0...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(200005)30:5<1340::AID-IMMU
更新日期:2000-05-01 00:00:00
abstract::Influenza matrix peptide 58-66 is shown to be the optimal nonamer for binding to HLA-A2 and presentation to cytotoxic T lymphocytes (CTL). If titered out to 2 x 10(-10) - 4 x 10(-10) M in CTL-mediated lysis assays and to 3 x 10(-9) M in an HLA-A2 assembly-stabilization assay in cell lysates. The peptide was shown to m...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220404
更新日期:1992-04-01 00:00:00
abstract::Antigen-specific human T lymphocytes were selected by adsorption of peripheral blood mononuclear cells on monolayers of antigen-treated autologous monocytes. The enriched cell populations were propagated in interleukin 2-containing medium for 60-80 days. Their activity was tested by a proliferative response against th...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830131116
更新日期:1983-11-01 00:00:00
abstract::Dopamine by itself has not up to now been reported to activate T cell function. We show here that dopamine interacts directly with dopaminergic receptors on normal human T cells and triggers beta1 integrin-mediated T cell adhesion to a major extracellular matrix component, fibronectin (FN). Such adhesion is a characte...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200112)31:12<3504::aid-immu3504>
更新日期:2001-12-01 00:00:00
abstract::Two different lines of C57BL/6 mice (IgHb) carrying complete rearranged mu chain genes from BALB/c (IgMa) were analyzed for the expression and secretion of endogenous as well as transgenic immunoglobulins at the level of single cells. Quantitation of B cells expressing endogenous IgMb by cytofluorometry, limiting dilu...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200507
更新日期:1990-05-01 00:00:00
abstract::DNA vaccines induce CTL attack on target tumor epitopes, but tumor elimination in vivo also requires sufficient effector CTL to enter the site, guided by inflammatory chemokines. Many herpesviruses contain genes for chemokine and chemokine receptor-like proteins to protect infected cells from immune attack. To assess ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200212)32:12<3481::AID-IMMU3481>
更新日期:2002-12-01 00:00:00
abstract::Cells in homeostasis metabolize glucose mainly through the tricarboxylic acid cycle and oxidative phosphorylation, while activated cells switch their basal metabolism to aerobic glycolysis. In this study, we examined whether metabolic reprogramming toward aerobic glycolysis is important for the host response to Mycoba...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201546259
更新日期:2016-11-01 00:00:00
abstract::Multinucleated giant cells (MGC), which are a common feature of various pathologic states, were generated in vitro by cytokine-stimulation of human peripheral blood monocytes. As expected, conditioned medium, i.e. the supernatant of concanavalin A-stimulated peripheral blood mononuclear cells, readily caused generatio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200807
更新日期:1990-08-01 00:00:00
abstract::Double-negative (DN) thymocyte subsets were examined in mice deficient in the CD3 zeta chain (zeta-/-). The HSA+CD44-CD25- subset was found to be missing, and DN thymocytes seemed to differentiate directly from HSA+CD25+CD44- cells to double-positive (DP) cells. When fetal thymic ontogeny was examined, we found a mark...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240828
更新日期:1994-08-01 00:00:00
abstract::Based upon the success of using polyclonal, Epstein-Barr virus (EBV)-specific CTL lines for the prophylaxis and treatment of patients with post-transplant lymphoproliferative disease (PTLPD), there is now considerable incentive to develop CTL directed against the sub-dominant EBV antigens EBNA1, LMP1 and LMP2, which a...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200103)31:3<947::aid-immu947>3.0
更新日期:2001-03-01 00:00:00
abstract::In this study we have used cells expressing LFA-3 or T11TS, the human and sheep forms of the ligand of CD2, as well as the purified LFA-3 and T11TS molecules themselves to study their effects on T cell activation via the CD2-mediated "alternative pathway". Sheep red blood cells, which bind to CD2 via T11TS in E-rosett...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171227
更新日期:1987-12-01 00:00:00
abstract::The specificity of the IgM receptor expressed by human T cells cultured in IgM-free media has been investigated. IgM receptors have been detected using a rosette system with ox erythrocytes coated with rabbit antibody (EA(IgM)), and the inhibitory capacity of different IgM fragments in the rosette system has been test...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830060714
更新日期:1976-07-01 00:00:00
abstract::The presence of dominant epitopes suppresses generation of CTL activity toward other non-dominant epitopes found on the same antigen-presenting cell (APC). This phenomenon, termed immunodomination, drastically restricts the diversity of the repertoire of CTL responses. Under various experimental conditions we assessed...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200108)31:8<2284::aid-immu2284>3
更新日期:2001-08-01 00:00:00
abstract::Cell suspensions enriched for N cells, the effector cell for human natural cell-mediated cytotoxicity, were observed to react strongly with a rabbit antiserum directed against an antigen associated with B cells. Treatment of the effector suspension with the antiserum resulted in inhibition of natural cell-mediated cyt...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830071016
更新日期:1977-10-01 00:00:00
abstract::The invariant chain (Ii) binds to newly synthesized major histocompatibility complex (MHC) class II molecules and is targeted to an acidic compartment where it is degraded. To evaluate its role on the conformation and the subcellular distribution of murine MHC class II molecules we have established stable L cell trans...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830231218
更新日期:1993-12-01 00:00:00
abstract::The adoptive transfer of CD4(+)CD25(+) natural regulatory T cells (Treg) is a promising strategy for the treatment of autoimmune diseases and the prevention of alloresponses after transplantation. Clinical trials exploring this strategy require efficient in vitro expansion of this rare cell population. Protocols devel...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838904
更新日期:2009-04-01 00:00:00
abstract::The NKG2D receptor is expressed by human NK, gammadelta T and alpha/beta T lymphocytes and its engagement results in the stimulation of effector cells. We evaluated the role of NKG2D receptor in anti-colorectal cancer (CRC) immune response. The cell surface expression of stress-inducible NKG2D ligands MICA/B (MHC clas...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200323909
更新日期:2003-07-01 00:00:00
abstract::Recent studies have suggested that T cell memory for recall antigens resides in clones of primed T cells with a short inter-mitotic half-life. In humans such cells express an isoform of the leukocyte common antigen termed CD45RO. Nevertheless, little is known of the fate of these primed T cells after initial activatio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240417
更新日期:1994-04-01 00:00:00
abstract::Several groups have described that a low percentage of in vitro cultured T cell receptor (TcR) gamma/delta cells express CD8. Contrary to TcR alpha/beta cells, however, CD8 on these TcR gamma/delta cells was shown to be a CD8 alpha homodimer. We describe here that addition of interleukin-7 (IL-7) to a short-term in vi...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220902
更新日期:1992-09-01 00:00:00
abstract::Both interleukin-7 (IL-7) and IL-7 receptor (R) gene knockout (IL-7-/- and IL-7R-/-) mice were employed in order to directly investigate the importance of the IL-7 and IL-7R signaling pathway for the development of intestinal intraepithelial lymphocytes (IEL). Loss of the IL-7R-specific gene resulted in complete defic...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270903
更新日期:1997-09-01 00:00:00
abstract::GTPase immune-associated proteins (Gimap) genes encode evolutionarily conserved GTP-binding proteins that are preferentially expressed in immune cells. Specific members have been shown to be involved in lymphocyte development, or are associated with inflammatory and autoimmune diseases. However, the function of these ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201344375
更新日期:2014-02-01 00:00:00
abstract::The cytokines interleukin (IL)-6, IL-11, ciliary neurotrophic factor (CNTF), leukemia inhibitor factor (LIF), oncostatin M (OSM) and probably the recently cloned cytokine cardiotrophin-1, signal, in combination with their specific receptors, through the common signal transducer gp130. Here, we report that the signalin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251240
更新日期:1995-12-01 00:00:00
abstract::There is limited insight into the mechanisms involved in the counterregulation of TLR. Given the important role of TLR3/TIR domain-containing adaptor-inducing IFN-β (TRIF)-dependent signalling in innate immunity, novel insights into its modulation is of significance in the context of many physiological and pathologica...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201040547
更新日期:2010-11-01 00:00:00
abstract::Using sheep erythrocytes (SRBC) as the antigen, two subpopulations of spleen antigen-binding lymphocytes could be distinguished by a marked difference in the susceptibility of their receptors to trypsin. In unimmunized animals, 30% of the antigen-binding cells were trypsin-resistant, whereas at 5 days after immunizati...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050612
更新日期:1976-06-01 00:00:00
abstract::Leishmaniasis is currently classified as category 1 disease, i.e. emerging and uncontrolled. Since the importance of persistent infection for maintaining an effective long-lasting protective response is controversial, the present study asks whether immunisation with non-persistent parasites leads to protection against...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838423
更新日期:2008-11-01 00:00:00