Abstract：
:The release of cytokines and chemokines from activated immune-competent cells plays a crucial role in determining the pathology of the atherogenic progress. We investigated the effect of bacterial lipopolysaccharide (LPS) on cytokine/chemokine expression in carotid lesions and normal renal arteries. The lesions or renal arteries were incubated for 6 h at 37 degrees C in serum-free media treated with or without LPS. After LPS treatment, increased protein levels of IL-1beta, IL-6, IL-8, IL-10, TNF-alpha and MCP-1 were observed in the culture medium from the lesions measured with cytometric bead array. We were able to detect the induction of IL-1beta, IL-6, IL-8, IL-10, TNF-alpha and MCP-1 mRNA in the lesions after stimulation with LPS using real-time PCR. In renal arteries, LPS also induces mRNA expression of all chemokines and cytokines investigated with the exception of IL-6. However, LPS induces significantly higher levels of TNF-alpha, IL-1beta and IL-10 mRNA in lesions compared to renal arteries. The results suggest that infectious agents are capable of enhancing the production of cytokines/chemokines in an already ongoing inflammatory process such as in atherosclerosis, and that low levels of circulating LPS may affect the levels of pro-inflammatory cytokines much more in atherosclerotic vessels than in normal vessels and may contribute to the development of the atherosclerotic lesion.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Jatta K,Wågsäter D,Norgren L,Stenberg B,Sirsjö Adoi
10.1159/000085721keywords:
subject
Has Abstractpub_date
2005-05-01 00:00:00pages
266-71issue
3eissn
1018-1172issn
1423-0135pii
85721journal_volume
42pub_type
杂志文章abstract：BACKGROUND:Infusion of exogenous vascular endothelial growth factor (VEGF) into adult brain at doses above 60 ng/day induces dramatic angiogenesis accompanied by vascular leak and inflammation. Blood vessels formed by this treatment are dilated and tortuous, exhibiting a pathological morphology. Pathological VEGF-induc...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000101450
更新日期：2007-01-01 00:00:00
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journal_title：Journal of vascular research
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000158987
更新日期：1993-05-01 00:00:00
abstract：:Fibroblast growth factor-2 (FGF2) and vascular endothelial growth factor (VEGF) exert their angiogenic activity by interacting with endothelial cells in a distinct manner. In this study, we investigated the morphological features of endothelial cells of the chick embryo chorioallantoic membrane (CAM) microvasculature ...
journal_title：Journal of vascular research
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doi：10.1159/000051070
更新日期：2001-07-01 00:00:00
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journal_title：Journal of vascular research
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journal_title：Journal of vascular research
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pub_type： 杂志文章,评审
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更新日期：2021-01-27 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000073902
更新日期：2003-09-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025592
更新日期：1998-07-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159196
更新日期：1997-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159145
更新日期：1996-03-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000090947
更新日期：2006-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000452410
更新日期：2016-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159077
更新日期：1995-01-01 00:00:00
abstract：BACKGROUND/AIMS:Endothelial nitric oxide synthase (eNOS) is associated with caveolin-1 (Cav-1) in plasma membrane. We tested the hypothesis that eNOS activation by shear stress in resistance vessels depends on synchronized phosphorylation, dissociation from Cav-1 and translocation of the membrane-bound enzyme to Golgi ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000355301
更新日期：2013-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000074550
更新日期：2003-09-01 00:00:00
abstract：:We investigated the possible interference of smooth muscle cells with monocyte response to LDL as well as with their adhesion and transmigration in a coculture of porcine endothelial and smooth muscle cells. Lysophosphatidylcholine (LPC), a component of oxidized LDL (oxLDL), stimulated the adhesion of THP-1 cells to e...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000051081
更新日期：2001-09-01 00:00:00
abstract：:Actions of the novel Ca2+ antagonist, Ro 40-5967, which displays unusual efficacy against endothelin (ET)-induced contractions, were studied in isolated vascular muscle cells (VMCs) using the fluorescent protein kinase C (PKC) indicator, BODIPY 12 alpha-phorbol ester 13 beta-acetate (PBA-BODIPY). High-sensitivity (pho...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159134
更新日期：1996-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000449059
更新日期：2016-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000051041
更新日期：2001-03-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000063687
更新日期：2002-05-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000097977
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000110208
更新日期：2008-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025753
更新日期：2000-09-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000135660
更新日期：2009-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章,评审
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更新日期：2011-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000226228
更新日期：2009-01-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159113
更新日期：1995-11-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025679
更新日期：1999-09-01 00:00:00
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journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000318777
更新日期：2011-01-01 00:00:00
