Abstract：
:In order to study the presence of sulphated glycoconjugates in the first mineralised layer juxtaposed to the root dentine (the hyaline layer), we have examined the early stages of molar root development by ultrastructural cytochemistry using Cuprolinic Blue combined with enzymatic pretreatment. Upper molars from 10 to 13 day-old Wistar rats were fixed in 2.5% glutaraldehyde containing 0.05% Cuprolinic Blue in 25 mM sodium acetate, pH 5.6, containing 0.3 M MgCl2. Some specimens were previously treated with heparitinase or chondroitinase ABC. Our results showed sulphated glycoconjugate--Cuprolinic Blue complexes that appeared as electron opaque ribbon-like deposits in the unmineralised hyaline layer. Few complexes were detected adjacent to the dentinal surface. These complexes were removed by heparitinase, indicating that they contained heparan sulphate chains. In contrast, the complexes found in unmineralised cementum and root dentine were removed by chondroitinase, indicating that they contained chondroitin or dermatan sulphate chains. The complexes decreased after the initiation of mineralisation of hyaline layer and root dentine and they were no longer present in stages of fully mineralisation. We conclude that the hyaline layer only contains sulphated glycoconjugates prior to mineralisation, and that they may play a role in the regulation of the mineralisation.
journal_name
J Mol Histoljournal_title
Journal of molecular histologyauthors
Tomazela-Herndl SA,Arana-Chavez VEdoi
10.1023/b:hijo.0000021089.75408.7ckeywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
63-8issue
1eissn
1567-2379issn
1567-2387journal_volume
35pub_type
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pub_type： 撤回出版物,收录出版
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