Abstract：
:DLBCL is the most common lymphoma subtype occurring in older populations as well as in younger HIV infected patients. The current treatment options for DLBCL are effective for most patients yet the relapse rate is high. While many biomarkers for DLBCL exist, they are not in clinical use due to low sensitivity and specificity. In addition, these biomarkers have not been studied in the HIV context. Therefore, the identification of new biomarkers for HIV negative and HIV positive DLBCL, may lead to a better understanding of the disease pathology and better therapeutic design. Protein biomarkers for DLBCL were determined using MALDI imaging mass spectrometry (IMS) and characterised using LC-MS. The expression of one of the biomarkers, heat shock protein (Hsp) 70, was confirmed on a separate cohort of samples using immunohistochemistry. The biomarkers identified in the study consisted of four protein clusters including glycolytic enzymes, ribosomal proteins, histones and collagen. These proteins could differentiate between control and tumour tissue, and the DLBCL immunohistochemical subtypes in both cohorts. The majority (41/52) of samples in the confirmation cohort were negative for Hsp70 expression. The HIV positive DLBCL cases had a higher percentage of cases expressing Hsp70 than their HIV negative counterparts. The non-GC subtype also frequently overexpressed Hsp70, confirming MALDI IMS data. The expression of Hsp70 did not correlate with survival in both the HIV negative and HIV positive cohort. This study identified potential biomarkers for HIV negative and HIV positive DLBCL from FFPE tissue sections. These may be used as diagnostic and prognostic markers complementary to current clinical management programmes for DLBCL.
journal_name
J Mol Histoljournal_title
Journal of molecular histologyauthors
Magangane P,Sookhayi R,Govender D,Naidoo Rdoi
10.1007/s10735-016-9695-ysubject
Has Abstractpub_date
2016-12-01 00:00:00pages
565-577issue
6eissn
1567-2379issn
1567-2387pii
10.1007/s10735-016-9695-yjournal_volume
47pub_type
杂志文章abstract：:The incidence of chronic renal diseases is increasing worldwide, and there is a great need to identify therapies capable of arresting or reducing disease progression. The current treatment of chronic nephropathies is limited to angiotensin-converting enzyme inhibitors and angiotensin receptor blockers, but increasing ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-011-9336-4
更新日期：2011-08-01 00:00:00
abstract：:Pulp regeneration using human dental pulp stem cells (hDPSCs) maintains tooth vitality compared with conventional root canal therapy. Our previous study demonstrated that preameloblast-conditioned medium (PA-CM) from murine apical bud cells induces the odontogenic differentiation of hDPSCs and promoted dentin formatio...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9513-8
更新日期：2013-12-01 00:00:00
abstract：:Human pluripotent stem cell (hPSC)-derived cardiomyocytes (CMs) hold great promise for the repair of the injured heart, but optimal cell production in a fully chemically defined and cost-effective system is essential for the efficacy and safety of cell transplantation therapies. In this study, we provided a simple and...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09926-0
更新日期：2021-02-01 00:00:00
abstract：:Objective Oligodendroglial tumors with a loss of heterozygosity of 1p (LOH1p) appear to have a better prognosis than oligodendrogliomas without LOH. Previously, we reported glucose uptake in low-grade oligodendroglial tumors to be related to LOH 1p status. Here, we performed an immunohistochemical study of the common ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-008-9191-0
更新日期：2008-10-01 00:00:00
abstract：:The cytoplasmic Cu/Zn-superoxide dismutase (SOD1) represents along with catalase and glutathione peroxidase at the first defense line against reactive oxygen species in all aerobic organisms, but little is known about its distribution in developing embryos. In this study, the expression patterns of SOD1 mRNA in mouse ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9134-1
更新日期：2008-02-01 00:00:00
abstract：:Follistatin like-1 (Fstl1) is a secreted glycoprotein and can be up-regulated by TGF-β1. To better study the function of Fstl1 in lung development, we examined Fstl1 expression in the developing lung, in a cell type specific manner, using a tamoxifen inducible Fstl1-reporter mouse strain. Our results show that Fstl1 i...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-018-9780-5
更新日期：2018-08-01 00:00:00
abstract：:Estrogens and their receptors are important factors involved in periodontal ligament (PDL) tissue health. As a regulator of estrogen receptors (ER), the proline-, glutamic acid-, and leucine-rich protein 1 (PELP1) may play a role in alveolar bone formation and PDL homeostasis. The aim of the present study was to obser...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9490-y
更新日期：2013-06-01 00:00:00
abstract：:MT1-MMP and TIMP-2 are well known for their roles in remodelling of extracellular matrix components. However, reports are emerging on the involvement of these molecules in cell kinetics. In the rat incisor tooth, a shortening treatment increases the eruption and cell proliferation rates. However, the role of MT1-MMP a...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-010-9295-1
更新日期：2010-12-01 00:00:00
abstract：:Wnt signaling plays important roles in skeletal development. However, the activation and function of canonical Wnt signaling in joint development remains unclear. We analyzed the lineage identity and developmental changes of the Wnt-responsive cells during synovial joint formation as well as adulthood in the Wnt signa...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-009-9242-1
更新日期：2009-08-01 00:00:00
abstract：:Elevated expression of CXCL9 has been shown to involve in the infiltration of inflammatory cells and liver damage after Hepatitis B virus (HBV) infection. However, whether and by what underlying mechanism does CXCL9 play a role in HBV infection associated hepatocellular carcinoma (HCC) invasion ability remain unclear....
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-014-9593-0
更新日期：2014-12-01 00:00:00
abstract：:Vesicular monoamine transporter 2 (VMAT2) is expressed in pancreatic beta cells and has recently been proposed as a target for measurement of beta cell mass in vivo. We questioned, (1) What proportion of beta cells express VMAT2? (2) Is VMAT2 expressed by other pancreatic endocrine or non-endocrine cells? (3) Is the r...
journal_title：Journal of molecular histology
pub_type： 杂志文章,多中心研究
doi：10.1007/s10735-008-9195-9
更新日期：2008-10-01 00:00:00
abstract：:Under normal homeostasis, melanocyte growth and behaviour is tightly controlled by the surrounding keratinocytes. Keratinocytes regulate melanocyte behaviour through a complex system of paracrine growth factors and cell-cell adhesion molecules. Pathological changes, leading to development of malignant melanoma, upset ...
journal_title：Journal of molecular histology
pub_type： 杂志文章,评审
doi：10.1023/b:hijo.0000032362.35354.bb
更新日期：2004-03-01 00:00:00
abstract：:Computational modelling of whole biological systems from cells to organs is gaining momentum in cell biology and disease studies. This pathway is essential for the derivation of explanatory frameworks that will facilitate the development of a predictive capacity for estimating outcomes or risk associated with particul...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-004-2653-0
更新日期：2004-09-01 00:00:00
abstract：:Some members of aquaporin family (AQP) plays crucial functions in salivary synthesis and secretion. These proteins expression has already been reported during salivary gland formation, however no previous studies in human developing glands have been performed. We evaluated AQP1, 3 and 5 expression through the stages o...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-017-9731-6
更新日期：2017-12-01 00:00:00
abstract：:In the last few decades, several growth factors were identified in the testis of various mammalian species. Growth factors are shown to promote cell proliferation, regulate tissue differentiation, and modulate organogenesis. In the present investigation we have studied the localization of EGF and EGFR in the adult bov...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9089-2
更新日期：2007-06-01 00:00:00
abstract：:Dentin is a major component of teeth that protects dental pulp and maintains tooth health. Bone morphogenetic protein (BMP) signaling is required for the formation of dentin. Mice lacking a BMP type I receptor, activin A receptor type 1 (ACVR1), in the neural crest display a deformed mandible. Acvr1 is known to be exp...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-018-9806-z
更新日期：2019-02-01 00:00:00
abstract：:Previous studies have shown aberrant expression of ubiquitin-specific protease 14 (USP14) in multiple malignancies, suggesting an important role of USP14 in tumorigenesis. However, the functional role of USP14 in pancreatic ductal adenocarcinoma (PDAC) has never been elucidated. In this study, we found that USP14 was ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09934-0
更新日期：2021-01-04 00:00:00
abstract：:Satellite glial cells (SGCs), a peripheral neuroglial cell, surround neurons and form a complete envelope around individual sensory neurons in the trigeminal ganglia (TG), which may be involved in modulating neurons in inflammation. The purpose of this study was to determine the effect of dental injury and inflammatio...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-018-9765-4
更新日期：2018-06-01 00:00:00
abstract：:The aim of this study was to ascertain whether, like many cell types in cartilaginous tissues if type XI collagen was a pericellular component of annulus fibrosus (AF) cells and chondrocytes. Fine fibrillar networks were visualised which were perlecan, HS (MAb 10E4) and type XI collagen positive. Heparitinase-III pre-...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09823-1
更新日期：2019-06-01 00:00:00
abstract：:MicroRNAs are small noncoding RNAs involved in various biological processes. We characterized the expression of miR-344-3p during mouse embryonic development. At E9.5-E10.5 and E15.5, in situ hybridization detected strong miR-344-3p signal in the central nervous system, including the cerebral cortex, hindbrain, cerebe...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9555-y
更新日期：2014-08-01 00:00:00
abstract：:Nel-like molecule-1 (NELL-1) is a novel highly specific growth factor that can induce osteoblast differentiation and bone formation as well as odontoblast differentiation. Recent studies have suggested that NELL-1 can synergistically increase bone formation and regeneration with bone morphogenetic protein 2 (BMP2) and...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09822-2
更新日期：2019-06-01 00:00:00
abstract：:Sphingosine-1-phosphate receptor 1 (S1P(1)), a receptor for sphingosine-1-phosphate, has been shown to play an important role in the migration, proliferation, and survival of several types of cell including endothelial cells. Given that S1P(1) signaling could serve as a therapeutic target, we evaluate the expression o...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-008-9193-y
更新日期：2008-10-01 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) is an important angiogenic factor in the pituitary gland. The objective of this study was to unveil the VEGF subcellular localisation in different pituitary cell types and to evaluate changes in its expression at different time intervals after oestrogen stimulation. A relevant...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-005-9012-7
更新日期：2005-10-01 00:00:00
abstract：:Cyclin G1 plays an essential role in the development of human carcinoma. Here, we characterized the clinical significance of Cyclin G1 and investigated its role in cellular proliferation and apoptosis of epithelial ovarian cancer (EOC). Western blot was used to evaluate the expression of Cyclin G1 in nine fresh EOC ti...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-015-9622-7
更新日期：2015-06-01 00:00:00
abstract：:Nickel nanoparticles (Ni NPs) have been applied in various fields along with the rapid development of nanotechnology. However, the potential adverse health effects of the Ni NPs are unclear. To investigate the cyto- and genotoxicity and compare the differences between the Ni NPs and the nickel fine particles (Ni FPs),...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-016-9671-6
更新日期：2016-06-01 00:00:00
abstract：:Angiotensin II (Ang II) has been reported to cause podocyte apoptosis in rats both in vivo and in vitro studies. However, the underlying mechanisms are poorly understood. In the present study, we investigated the role of the nonreceptor tyrosine kinase c-Abl in Ang II-induced podocyte apoptosis. Male Sprague-Dawley ra...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9505-8
更新日期：2013-10-01 00:00:00
abstract：:E3 ubiquitin ligases regulate a variety of biological processes through the ubiquitin-proteasome system, together with ubiquitin activating enzyme E1 and ubiquitin-conjugating enzyme E2. Previous studies have demonstrated that zinc and ring finger 3 (ZNRF3), which belongs to the E3 ubiquitin ligases family is involved...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9504-9
更新日期：2013-10-01 00:00:00
abstract：:Recent studies have shown that chronic endothelial dysfunction can impair multiple aspects of renal physiology and, in turn, contribute to renal fibrosis. Sphingosine 1-phosphate (S1P) has been highlighted as an endothelial barrier-stabilizing mediator. The aim of our study was to investigate the effect of FTY720, an ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9521-8
更新日期：2013-12-01 00:00:00
abstract：:Five subtypes of dopamine receptor exist in two subfamilies: two D(1)-like (D(1) and D(5)) and three D(2)-like (D(2), D(3) and D(4)). We produced novel monoclonal antibodies against all three D(2)-like receptors and used them to localize receptors in Ntera-2 (NT-2) cells, the human neuronal precursor cell line. Most o...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9106-5
更新日期：2007-08-01 00:00:00
abstract：:The first cell lineage differentiation occurs during the development of mouse 8-cell embryo to blastocyst. Akt is a potent kinase whose role during blastocyst formation has not been elucidated. In the present study, immunofluorescence results showed that the Akt protein was specifically localized to the outer cells of...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09833-z
更新日期：2019-08-01 00:00:00
