Abstract：
:Vascular endothelial growth factor (VEGF) is an important angiogenic factor in the pituitary gland. The objective of this study was to unveil the VEGF subcellular localisation in different pituitary cell types and to evaluate changes in its expression at different time intervals after oestrogen stimulation. A relevant feature demonstrated was the identification of this cytokine in the nucleus and cytoplasm of lactotrophs, somatotrophs and gonadotrophs, as well as in follicle-stellate cells of male rats. Oestrogen treatment increased the number of VEGF immunopositive cells and its expression detected differentially by western blot in both nucleus and cytoplasm of pituitary cells when compared to the control. At ultrastructural level VEGF appeared associated with nucleolus and euchromatin involving a possible internal autocrine loop. In lactotrophs, the predominant cell of the tumour, VEGF was immunodetected in RER, Golgi complex, and vesicular organelles, supporting further the association with an auto-paracrine effect exerted by VEGF. The nucleus/cytoplasm ratio of VEGF revealed a prevalent accumulation of VEGF in the cytoplasm. The presence of VEGF in the nucleus may probably be associated with a translocation to this cell compartment. This study demonstrated a cytoplasmic and nuclear immunolocalisation of VEGF in normal and tumoural adenohypophyseal cells. In the course of prolactinoma development, the oestrogen stimulated VEGF expression in tumoural cells, promoting a vascular adaptation which contributes to growth and progression of the tumour.
journal_name
J Mol Histoljournal_title
Journal of molecular histologyauthors
Mukdsi JH,De Paul AL,Gutiérrez S,Roth FD,Aoki A,Torres AIdoi
10.1007/s10735-005-9012-7subject
Has Abstractpub_date
2005-10-01 00:00:00pages
447-54issue
8-9eissn
1567-2379issn
1567-2387journal_volume
36pub_type
杂志文章abstract：:Selective cyclooxygenase-2 non-steroidal anti-inflammatory drugs are known to inhibit bone repair, especially when long-term administration is required due to chronicle inflammatory diseases. In order to evaluate the action of this drug in bone repair during short-term administration, 48 rats underwent surgical bone d...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-008-9176-z
更新日期：2008-08-01 00:00:00
abstract：:Four and a half LIM domains 2 (FHL2) is a protein of 279 amino acids in length containing four full LIM-domains and a half LIM-domain at the amino terminus. FHL2 is one transcriptional cofactor that can interact with many different proteins, such as AP-1, BRCA1, IGFBP, and integrin, and involved in organ differentiati...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-016-9654-7
更新日期：2016-04-01 00:00:00
abstract：:Wnt signaling plays important roles in skeletal development. However, the activation and function of canonical Wnt signaling in joint development remains unclear. We analyzed the lineage identity and developmental changes of the Wnt-responsive cells during synovial joint formation as well as adulthood in the Wnt signa...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-009-9242-1
更新日期：2009-08-01 00:00:00
abstract：:Sphingosine-1-phosphate receptor 1 (S1P(1)), a receptor for sphingosine-1-phosphate, has been shown to play an important role in the migration, proliferation, and survival of several types of cell including endothelial cells. Given that S1P(1) signaling could serve as a therapeutic target, we evaluate the expression o...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-008-9193-y
更新日期：2008-10-01 00:00:00
abstract：:Heparan sulfate proteoglycans (HSPGs) are complex and labile macromolecular moieties on the surfaces of cells that control the activities of a range of extracellular proteins, particularly those driving growth and regeneration. Here, we examine the biosynthesis of heparan sulfate (HS) sugars produced by cultured MC3T3...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9123-4
更新日期：2007-10-01 00:00:00
abstract：:DLBCL is the most common lymphoma subtype occurring in older populations as well as in younger HIV infected patients. The current treatment options for DLBCL are effective for most patients yet the relapse rate is high. While many biomarkers for DLBCL exist, they are not in clinical use due to low sensitivity and spec...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-016-9695-y
更新日期：2016-12-01 00:00:00
abstract：:Odontogenic keratocysts (OKCs) are jaw cystic lesions which are characterized by local invasion and high recurrence rate. The majority of OKCs are exposed to microorganisms and occur along with focal inflammatory infiltrates. Cyst fluids are biological fluids that contain a large content of cytokines and immune globul...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09829-9
更新日期：2019-08-01 00:00:00
abstract：:The accurately establishment of the eruptive pathway is of vital importance. The mechanisms governing tooth eruption pathway remain little known. This study is to elucidate the roles of Semaphorin 3A (Sema 3A) in mouse tooth eruptive pathway. C57BL/6 mice (11-13 and 15-17 days after birth) were chosen to observe erupt...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09838-8
更新日期：2019-10-01 00:00:00
abstract：:The aim of this study was to investigate oxidative DNA damage during 4-nitroquinoline 1-oxide (4NQO)-induced rat tongue carcinogenesis. For this purpose, male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12, and 20 weeks. Te...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-011-9323-9
更新日期：2011-04-01 00:00:00
abstract：:This study aimed to explore whether the altered expression of tumor necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF) and apoptotic changes in mid zone (MZ) and rupture zone (RZ) of fetal membranes (FM) are regulatory mechanisms associated with labor at term. Fifteen FM specimens were collected ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9553-0
更新日期：2014-06-01 00:00:00
abstract：:Elevated expression of CXCL9 has been shown to involve in the infiltration of inflammatory cells and liver damage after Hepatitis B virus (HBV) infection. However, whether and by what underlying mechanism does CXCL9 play a role in HBV infection associated hepatocellular carcinoma (HCC) invasion ability remain unclear....
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-014-9593-0
更新日期：2014-12-01 00:00:00
abstract：:Immunohistochemical and fluorescent in situ hybridization (FISH) assays are useful diagnostic methods for the identification of bacteria on formalin fixed paraffin embedded histological sections. To validate an anti-bacterial antibody or an oligonucleotide probe and to ensure fidelity during subsequent analyses, suita...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-008-9173-2
更新日期：2008-08-01 00:00:00
abstract：:Nickel nanoparticles (Ni NPs) have been applied in various fields along with the rapid development of nanotechnology. However, the potential adverse health effects of the Ni NPs are unclear. To investigate the cyto- and genotoxicity and compare the differences between the Ni NPs and the nickel fine particles (Ni FPs),...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-016-9671-6
更新日期：2016-06-01 00:00:00
abstract：:S100A8 and S100A9 are calcium-binding proteins expressed in myeloid cells and are markers of numerous inflammatory diseases in humans. S100A9 has been associated with dystrophic calcification in human atherosclerosis. Here we demonstrate S100A8 and S100A9 expression in murine and human bone and cartilage cells. Only S...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9117-2
更新日期：2007-10-01 00:00:00
abstract：:Uterine Natural Killer (uNK) cells are the most abundant lymphocyte population recruited in the uteri during murine and human pregnancy. Previous investigation on uNK cells during mouse pregnancy focused more on its accumulation in postimplantation periods, which were believed to play important roles in regulating tro...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-010-9256-8
更新日期：2010-02-01 00:00:00
abstract：:The cytoplasmic Cu/Zn-superoxide dismutase (SOD1) represents along with catalase and glutathione peroxidase at the first defense line against reactive oxygen species in all aerobic organisms, but little is known about its distribution in developing embryos. In this study, the expression patterns of SOD1 mRNA in mouse ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9134-1
更新日期：2008-02-01 00:00:00
abstract：:p53 plays a pivotal role in the prevention of human tumor formation. p73 and p63 are new members of the p53 tumor suppressor family, which are becoming increasingly recognized as important players in human tumorigenesis. However, the roles of these proteins are not well elucidated in extrahepatic bile duct (EBD) carci...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9084-7
更新日期：2007-06-01 00:00:00
abstract：:MI-ER1 is a novel transcriptional regulator that plays a critical role in embryonic development and is differentially expressed in breast carcinoma. The MI-ER1 protein sequence is highly conserved among species, with 95% identity between mouse and humans and 72% between Xenopus and mouse. There are two major protein i...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9116-3
更新日期：2008-02-01 00:00:00
abstract：:Adipose-derived mesenchymal stem cell (ADSC) with a high capacity of chondrogenic differentiation was a promising candidate for cartilage defect treatment. This study's objective is to study the roles of integrin β1 (ITGB1) in regulating ADSC chondrogenic differentiations as well as the underlying mechanisms. The iden...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09918-0
更新日期：2020-12-01 00:00:00
abstract：:The members of high-temperature requirement (HtrA) family are evolutionarily conserved serine proteases that combine a trypsin-like protease domain with at least one PDZ interaction domain. HtrA2, a special one, is mainly located in mitochondria and required for maintaining homeostasis. Once released into cytoplasm, H...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9489-4
更新日期：2013-08-01 00:00:00
abstract：:Human pluripotent stem cell (hPSC)-derived cardiomyocytes (CMs) hold great promise for the repair of the injured heart, but optimal cell production in a fully chemically defined and cost-effective system is essential for the efficacy and safety of cell transplantation therapies. In this study, we provided a simple and...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09926-0
更新日期：2021-02-01 00:00:00
abstract：:Submandibular gland (SMG) autotransplantation is an effective therapy for treating severe dry eye syndrome. However, epiphora occurs in more than 40% of patients 6 months after operation. We previously found that muscarinic acetylcholine receptor (mAChR) plays a crucial role in regulating SMG secretion partially throu...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-016-9709-9
更新日期：2017-04-01 00:00:00
abstract：:Spinal cord injury frequently results in permanent loss of neurological function. It includes many complex molecular and biochemical mechanisms. G-protein-coupled receptor kinase 6 (GRK6) is an intracellular kinase that regulates the sensitivity of certain G-protein-coupled receptors. Some studies reported GRK2 and GR...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9486-7
更新日期：2013-06-01 00:00:00
abstract：:Estrogens and their receptors are important factors involved in periodontal ligament (PDL) tissue health. As a regulator of estrogen receptors (ER), the proline-, glutamic acid-, and leucine-rich protein 1 (PELP1) may play a role in alveolar bone formation and PDL homeostasis. The aim of the present study was to obser...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9490-y
更新日期：2013-06-01 00:00:00
abstract：:During transmyocardial revascularization, cellular destruction of cardiomyocytes occurs as a result of the high-energy laser. However, the features of myocardial cellular destruction are unclear. The present study was undertaken to examine the structural characteristics of cell death in the myocardium following transm...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-005-5343-7
更新日期：2005-05-01 00:00:00
abstract：:The aim of this study was to ascertain whether, like many cell types in cartilaginous tissues if type XI collagen was a pericellular component of annulus fibrosus (AF) cells and chondrocytes. Fine fibrillar networks were visualised which were perlecan, HS (MAb 10E4) and type XI collagen positive. Heparitinase-III pre-...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09823-1
更新日期：2019-06-01 00:00:00
abstract：:p21-activated Kinase 6 (PAK6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. PAK kinases are well-known regulators of a wide variety of cellular functions, including regulation of cytoskeleton rearrangement, cell survival, apoptosis and the mitogen-activated protein kinase signaling p...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-011-9324-8
更新日期：2011-06-01 00:00:00
abstract：:Low muscle strength is observed during the peri-and postmenopausal periods, when the secretion of ovarian hormones is drastically reduced. It is also a predictive of adverse health events as well as incident mobility limitation and disability. The objective of the present study is to study the biochemical and the hist...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-011-9326-6
更新日期：2011-06-01 00:00:00
abstract：:Bone marrow-derived mesenchymal stem cells (BMMSCs) transplantation has shown to be effective in treating chronic kidney disease. However, the effectiveness of this strategy is constrained by low homing and survival rate of transplanted cells in the injured organs. Therefore, developing strategies to improve homing an...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09812-4
更新日期：2019-04-01 00:00:00
abstract：:Pulp regeneration using human dental pulp stem cells (hDPSCs) maintains tooth vitality compared with conventional root canal therapy. Our previous study demonstrated that preameloblast-conditioned medium (PA-CM) from murine apical bud cells induces the odontogenic differentiation of hDPSCs and promoted dentin formatio...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9513-8
更新日期：2013-12-01 00:00:00
