Abstract：
:p21-activated Kinase 6 (PAK6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. PAK kinases are well-known regulators of a wide variety of cellular functions, including regulation of cytoskeleton rearrangement, cell survival, apoptosis and the mitogen-activated protein kinase signaling pathway. To elucidate the expressions and possible functions of PAK6 in central nervous system (CNS) lesion and repair, we performed a traumatic brain injury (TBI) model in adult rats. Western blot analysis revealed that PAK6 level significantly increased at day 3 after damage, and then declined during the following days. Besides, double immunofluorescence staining showed PAK6 was primarily expressed in the neurons and a few of glial cells in the normal group. While after injury, the expression of PAK6 was increased significantly in the astrocytes and neurons, and the astrocytes had largely proliferated. We also examined the expression of proliferating cell nuclear antigen (PCNA) whose change was correlated with the expression of PAK6. Importantly, double immunofluorescence staining revealed that cell proliferation evaluated by PCNA appeared in many PAK6-expressing cells at day 3 after injury. In addition, injury-induced expression of PAK6 was co-labeled by active caspase-3 during neuronal apoptosis after injury. Collectively, we hypothesized PAK6 may play important roles in CNS pathophysiology after TBI and further research is needed to have a good understanding of its function and mechanism.
journal_name
J Mol Histoljournal_title
Journal of molecular histologyauthors
Zhao W,Yang J,Shi W,Wu X,Shao B,Wu Q,Chen J,Ni Ldoi
10.1007/s10735-011-9324-8subject
Has Abstractpub_date
2011-06-01 00:00:00pages
195-203issue
3eissn
1567-2379issn
1567-2387journal_volume
42pub_type
杂志文章abstract：:Inpp5f-v3 is a transcriptional variant of Inpp5f (inositol polyphosphate-5-phosphatase F) and locates in distal mouse chromosome 7. It is a paternally expressed imprinted gene in mouse. In this study, we examined the spatiotemporal patterns of Inpp5f-v3 gene during the mouse development. The northern blotting analysis...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-011-9321-y
更新日期：2011-04-01 00:00:00
abstract：:Cyclin G1 plays an essential role in the development of human carcinoma. Here, we characterized the clinical significance of Cyclin G1 and investigated its role in cellular proliferation and apoptosis of epithelial ovarian cancer (EOC). Western blot was used to evaluate the expression of Cyclin G1 in nine fresh EOC ti...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-015-9622-7
更新日期：2015-06-01 00:00:00
abstract：:Bone marrow-derived mesenchymal stem cells (BMMSCs) transplantation has shown to be effective in treating chronic kidney disease. However, the effectiveness of this strategy is constrained by low homing and survival rate of transplanted cells in the injured organs. Therefore, developing strategies to improve homing an...
journal_title：Journal of molecular histology
pub_type： 杂志文章
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更新日期：2019-04-01 00:00:00
abstract：:Human pluripotent stem cell (hPSC)-derived cardiomyocytes (CMs) hold great promise for the repair of the injured heart, but optimal cell production in a fully chemically defined and cost-effective system is essential for the efficacy and safety of cell transplantation therapies. In this study, we provided a simple and...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09926-0
更新日期：2021-02-01 00:00:00
abstract：:Gekko japonicus undergoes dramatic changes in the caudal spinal cord after tail amputation. The amputation induces cell proliferation in the caudal ependymal tube. We performed hematoxylin and eosin staining at different time points in the regeneration process to investigate the morphological characterization of the r...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-012-9466-3
更新日期：2013-06-01 00:00:00
abstract：:With the marker of Paneth cells-lysozyme, secretory component (SC) immunoreactivity was demonstrated exclusively in Paneth cells of rat small intestine. The other types of epithelial cells (columnar, goblet, endocrine) were negative. On electron microscopic level, many SC-positive colloidal gold particles were found i...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-005-9003-8
更新日期：2005-06-01 00:00:00
abstract：:Despite the efficacy of tamoxifen in preventing disease relapse, a large portion of breast cancer patients show intrinsic or acquired resistance to tamoxifen, leading to treatment failure and unfavorable clinical outcome. MYB proto-oncogene like 2 (MYBL2) is a transcription factor implicated in the initiation and prog...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09920-6
更新日期：2021-02-01 00:00:00
abstract：:MicroRNAs are small noncoding RNAs involved in various biological processes. We characterized the expression of miR-344-3p during mouse embryonic development. At E9.5-E10.5 and E15.5, in situ hybridization detected strong miR-344-3p signal in the central nervous system, including the cerebral cortex, hindbrain, cerebe...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9555-y
更新日期：2014-08-01 00:00:00
abstract：:The accurately establishment of the eruptive pathway is of vital importance. The mechanisms governing tooth eruption pathway remain little known. This study is to elucidate the roles of Semaphorin 3A (Sema 3A) in mouse tooth eruptive pathway. C57BL/6 mice (11-13 and 15-17 days after birth) were chosen to observe erupt...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09838-8
更新日期：2019-10-01 00:00:00
abstract：:Uterine Natural Killer (uNK) cells are the most abundant lymphocyte population recruited in the uteri during murine and human pregnancy. Previous investigation on uNK cells during mouse pregnancy focused more on its accumulation in postimplantation periods, which were believed to play important roles in regulating tro...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-010-9256-8
更新日期：2010-02-01 00:00:00
abstract：:Within the reproductive system, oestrogenic stimulation of uterine and pituitary tissue typically causes a proliferative response accompanied by an angiogenic induction of new blood vessels from existing ones, thereby providing nutrients and oxygen to the growing tissue. The pro-oestrogenic pesticide methoxychlor (MXC...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1023/b:hijo.0000039839.17945.89
更新日期：2004-05-01 00:00:00
abstract：:Nel-like molecule-1 (NELL-1) is a novel highly specific growth factor that can induce osteoblast differentiation and bone formation as well as odontoblast differentiation. Recent studies have suggested that NELL-1 can synergistically increase bone formation and regeneration with bone morphogenetic protein 2 (BMP2) and...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09822-2
更新日期：2019-06-01 00:00:00
abstract：:Low muscle strength is observed during the peri-and postmenopausal periods, when the secretion of ovarian hormones is drastically reduced. It is also a predictive of adverse health events as well as incident mobility limitation and disability. The objective of the present study is to study the biochemical and the hist...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-011-9326-6
更新日期：2011-06-01 00:00:00
abstract：:Spinal cord injury frequently results in permanent loss of neurological function. It includes many complex molecular and biochemical mechanisms. G-protein-coupled receptor kinase 6 (GRK6) is an intracellular kinase that regulates the sensitivity of certain G-protein-coupled receptors. Some studies reported GRK2 and GR...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9486-7
更新日期：2013-06-01 00:00:00
abstract：:As one of the most common cancers in female, ovarian cancer (OC) has become a serious public burden now. Mounting researches have indicated long noncoding RNAs (lncRNAs) can affect many biological processes including cancer development. LncRNA LBX2-AS1 was identified to be an oncogene in some cancers, but the role of ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09921-5
更新日期：2021-02-01 00:00:00
abstract：:Osteoprotegerin (OPG) and the receptor activator of nuclear factor (NF)-kB ligand (RANKL) are key regulators of osteoclastogenesis. The present study had the main aim of showing the localization of OPG and RANKL mRNA and protein in serial sections of the rat femurs and tibiae by immunohistochemistry (IHC) and in situ ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-004-3839-1
更新日期：2005-02-01 00:00:00
abstract：:In in vitro and in vivo systems, understanding localization and the functional role of ATP is essential, but effective methods to monitor ATP in cells and tissues are limited. Although quinacrine dihydrochloride is a well-known fluorescent dye used to detect ATP, it is limited in its use because it shows non-specific ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-012-9474-3
更新日期：2013-04-01 00:00:00
abstract：:The members of high-temperature requirement (HtrA) family are evolutionarily conserved serine proteases that combine a trypsin-like protease domain with at least one PDZ interaction domain. HtrA2, a special one, is mainly located in mitochondria and required for maintaining homeostasis. Once released into cytoplasm, H...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9489-4
更新日期：2013-08-01 00:00:00
abstract：:Dentin is a major component of teeth that protects dental pulp and maintains tooth health. Bone morphogenetic protein (BMP) signaling is required for the formation of dentin. Mice lacking a BMP type I receptor, activin A receptor type 1 (ACVR1), in the neural crest display a deformed mandible. Acvr1 is known to be exp...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-018-9806-z
更新日期：2019-02-01 00:00:00
abstract：:During human pregnancy the placenta produces a variety of proteins like steroid hormones and their receptors that are responsible for the establishment and ongoing of the feto-placental unit. Inhibins are dimeric glycoproteins, composed of an alpha-subunit and one of two possible beta-subunits (beta (A) or beta (B)). ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-006-9029-6
更新日期：2006-01-01 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) is an important angiogenic factor in the pituitary gland. The objective of this study was to unveil the VEGF subcellular localisation in different pituitary cell types and to evaluate changes in its expression at different time intervals after oestrogen stimulation. A relevant...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-005-9012-7
更新日期：2005-10-01 00:00:00
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journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9553-0
更新日期：2014-06-01 00:00:00
abstract：:Previous studies demonstrate significant roles for passive water channels (aquaporins, AQPs) in maintaining water homeostasis in cell membranes of endometrial cells during decidualisation and embryo implantation. However, there is little information regarding the role of AQPs in the human fallopian tube, specifically ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-012-9471-6
更新日期：2013-04-01 00:00:00
abstract：:Recent studies have shown that chronic endothelial dysfunction can impair multiple aspects of renal physiology and, in turn, contribute to renal fibrosis. Sphingosine 1-phosphate (S1P) has been highlighted as an endothelial barrier-stabilizing mediator. The aim of our study was to investigate the effect of FTY720, an ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9521-8
更新日期：2013-12-01 00:00:00
abstract：:Under normal homeostasis, melanocyte growth and behaviour is tightly controlled by the surrounding keratinocytes. Keratinocytes regulate melanocyte behaviour through a complex system of paracrine growth factors and cell-cell adhesion molecules. Pathological changes, leading to development of malignant melanoma, upset ...
journal_title：Journal of molecular histology
pub_type： 杂志文章,评审
doi：10.1023/b:hijo.0000032362.35354.bb
更新日期：2004-03-01 00:00:00
abstract：:Heparan sulfate proteoglycans (HSPGs) are complex and labile macromolecular moieties on the surfaces of cells that control the activities of a range of extracellular proteins, particularly those driving growth and regeneration. Here, we examine the biosynthesis of heparan sulfate (HS) sugars produced by cultured MC3T3...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9123-4
更新日期：2007-10-01 00:00:00
abstract：:Neutrotrophins are increasingly appreciated as potential modulators of neuronal function in the adult central nervous system (CNS). To describe the neurotrophin environment within the adult CNS, mRNA and protein expression patterns of neurotrophins-3 and -4 and of brain-derived neurotrophin were investigated in adult ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-004-0778-9
更新日期：2004-11-01 00:00:00
abstract：AIM:To compare the expression of p57 as indirect marker of genomic imprinting of CDKN1C in a series of infantile hemangiomas (IH) of patients with and without Beckwith-Wiedemann syndrome. MATERIALS AND METHODS:Cases of mammary, salivary gland, liver (one each), and placental (2 cases) capillary hemangiomas all with hi...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-005-2255-5
更新日期：2005-03-01 00:00:00
abstract：:Craniofacial autologous bone grafts offer superior outcomes to long bone grafts in the reconstruction of maxillofacial bone defects, but the mechanism responsible for this superiority has not yet been illustrated clearly. Osteoblasts play vital roles in bone development and regeneration. However, presently, only a few...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09810-6
更新日期：2019-04-01 00:00:00
abstract：:Previous studies have shown aberrant expression of ubiquitin-specific protease 14 (USP14) in multiple malignancies, suggesting an important role of USP14 in tumorigenesis. However, the functional role of USP14 in pancreatic ductal adenocarcinoma (PDAC) has never been elucidated. In this study, we found that USP14 was ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09934-0
更新日期：2021-01-04 00:00:00
