Abstract：
:Heparan sulfate proteoglycans (HSPGs) are complex and labile macromolecular moieties on the surfaces of cells that control the activities of a range of extracellular proteins, particularly those driving growth and regeneration. Here, we examine the biosynthesis of heparan sulfate (HS) sugars produced by cultured MC3T3-E1 mouse calvarial pre-osteoblast cells in order to explore the idea that changes in HS activity in turn drive phenotypic development during osteogenesis. Cells grown for 5 days under proliferating conditions were compared to cells grown for 20 days under mineralizing conditions with respect to their phenotype, the forms of HS core protein produced, and their HS sulfotransferase biosynthetic enzyme levels. RQ-PCR data was supported by the results from the purification of day 5 and day 20 HS forms by anionic exchange chromatography. The data show that cells in active growth phases produce more complex forms of sugar than cells that have become relatively quiescent during active mineralization, and that these in turn can differentially influence rates of cell growth when added exogenously back to preosteoblasts.
journal_name
J Mol Histoljournal_title
Journal of molecular histologyauthors
Nurcombe V,Goh FJ,Haupt LM,Murali S,Cool SMdoi
10.1007/s10735-007-9123-4subject
Has Abstractpub_date
2007-10-01 00:00:00pages
469-81issue
5eissn
1567-2379issn
1567-2387journal_volume
38pub_type
杂志文章abstract：:Bone marrow-derived mesenchymal stem cells (BMMSCs) transplantation has shown to be effective in treating chronic kidney disease. However, the effectiveness of this strategy is constrained by low homing and survival rate of transplanted cells in the injured organs. Therefore, developing strategies to improve homing an...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09812-4
更新日期：2019-04-01 00:00:00
abstract：:Pulp regeneration using human dental pulp stem cells (hDPSCs) maintains tooth vitality compared with conventional root canal therapy. Our previous study demonstrated that preameloblast-conditioned medium (PA-CM) from murine apical bud cells induces the odontogenic differentiation of hDPSCs and promoted dentin formatio...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9513-8
更新日期：2013-12-01 00:00:00
abstract：:Tissue microarrays have become an essential tool in translational pathology. They are used to confirm results from other experimental platforms, such as expression microarrays, as well as a primary tool to explore the expression profile of proteins by immunohistochemical analysis. Tissue microarrays are routinely used...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-006-9049-2
更新日期：2007-05-01 00:00:00
abstract：:As one of the most common cancers in female, ovarian cancer (OC) has become a serious public burden now. Mounting researches have indicated long noncoding RNAs (lncRNAs) can affect many biological processes including cancer development. LncRNA LBX2-AS1 was identified to be an oncogene in some cancers, but the role of ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09921-5
更新日期：2021-02-01 00:00:00
abstract：:The aim of this study was to ascertain whether, like many cell types in cartilaginous tissues if type XI collagen was a pericellular component of annulus fibrosus (AF) cells and chondrocytes. Fine fibrillar networks were visualised which were perlecan, HS (MAb 10E4) and type XI collagen positive. Heparitinase-III pre-...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09823-1
更新日期：2019-06-01 00:00:00
abstract：:Low muscle strength is observed during the peri-and postmenopausal periods, when the secretion of ovarian hormones is drastically reduced. It is also a predictive of adverse health events as well as incident mobility limitation and disability. The objective of the present study is to study the biochemical and the hist...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-011-9326-6
更新日期：2011-06-01 00:00:00
abstract：:During human pregnancy the placenta produces a variety of proteins like steroid hormones and their receptors that are responsible for the establishment and ongoing of the feto-placental unit. Inhibins are dimeric glycoproteins, composed of an alpha-subunit and one of two possible beta-subunits (beta (A) or beta (B)). ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-006-9029-6
更新日期：2006-01-01 00:00:00
abstract：:Recent studies have shown that chronic endothelial dysfunction can impair multiple aspects of renal physiology and, in turn, contribute to renal fibrosis. Sphingosine 1-phosphate (S1P) has been highlighted as an endothelial barrier-stabilizing mediator. The aim of our study was to investigate the effect of FTY720, an ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9521-8
更新日期：2013-12-01 00:00:00
abstract：:Multidrug resistance-associated protein 1 (MRP1) belongs to ATP-binding cassette transporters family. The overexpression of MRP1 is predominantly related with the failure of chemo-radiotherapy in various tumors. However, its possible role in hypertrophic scar (HS) is hardly investigated. Here we showed that the mRNA l...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-015-9629-0
更新日期：2015-10-01 00:00:00
abstract：:Dentin is a major component of teeth that protects dental pulp and maintains tooth health. Bone morphogenetic protein (BMP) signaling is required for the formation of dentin. Mice lacking a BMP type I receptor, activin A receptor type 1 (ACVR1), in the neural crest display a deformed mandible. Acvr1 is known to be exp...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-018-9806-z
更新日期：2019-02-01 00:00:00
abstract：AIM:To compare the expression of p57 as indirect marker of genomic imprinting of CDKN1C in a series of infantile hemangiomas (IH) of patients with and without Beckwith-Wiedemann syndrome. MATERIALS AND METHODS:Cases of mammary, salivary gland, liver (one each), and placental (2 cases) capillary hemangiomas all with hi...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-005-2255-5
更新日期：2005-03-01 00:00:00
abstract：:In the last few decades, several growth factors were identified in the testis of various mammalian species. Growth factors are shown to promote cell proliferation, regulate tissue differentiation, and modulate organogenesis. In the present investigation we have studied the localization of EGF and EGFR in the adult bov...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9089-2
更新日期：2007-06-01 00:00:00
abstract：:S100A8 and S100A9 are calcium-binding proteins expressed in myeloid cells and are markers of numerous inflammatory diseases in humans. S100A9 has been associated with dystrophic calcification in human atherosclerosis. Here we demonstrate S100A8 and S100A9 expression in murine and human bone and cartilage cells. Only S...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9117-2
更新日期：2007-10-01 00:00:00
abstract：:Gekko japonicus undergoes dramatic changes in the caudal spinal cord after tail amputation. The amputation induces cell proliferation in the caudal ependymal tube. We performed hematoxylin and eosin staining at different time points in the regeneration process to investigate the morphological characterization of the r...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-012-9466-3
更新日期：2013-06-01 00:00:00
abstract：:Elevated expression of CXCL9 has been shown to involve in the infiltration of inflammatory cells and liver damage after Hepatitis B virus (HBV) infection. However, whether and by what underlying mechanism does CXCL9 play a role in HBV infection associated hepatocellular carcinoma (HCC) invasion ability remain unclear....
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-014-9593-0
更新日期：2014-12-01 00:00:00
abstract：:Neutrotrophins are increasingly appreciated as potential modulators of neuronal function in the adult central nervous system (CNS). To describe the neurotrophin environment within the adult CNS, mRNA and protein expression patterns of neurotrophins-3 and -4 and of brain-derived neurotrophin were investigated in adult ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-004-0778-9
更新日期：2004-11-01 00:00:00
abstract：:Follistatin like-1 (Fstl1) is a secreted glycoprotein and can be up-regulated by TGF-β1. To better study the function of Fstl1 in lung development, we examined Fstl1 expression in the developing lung, in a cell type specific manner, using a tamoxifen inducible Fstl1-reporter mouse strain. Our results show that Fstl1 i...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-018-9780-5
更新日期：2018-08-01 00:00:00
abstract：:Some members of aquaporin family (AQP) plays crucial functions in salivary synthesis and secretion. These proteins expression has already been reported during salivary gland formation, however no previous studies in human developing glands have been performed. We evaluated AQP1, 3 and 5 expression through the stages o...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-017-9731-6
更新日期：2017-12-01 00:00:00
abstract：:p21-activated Kinase 6 (PAK6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. PAK kinases are well-known regulators of a wide variety of cellular functions, including regulation of cytoskeleton rearrangement, cell survival, apoptosis and the mitogen-activated protein kinase signaling p...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-011-9324-8
更新日期：2011-06-01 00:00:00
abstract：:Sphingosine-1-phosphate receptor 1 (S1P(1)), a receptor for sphingosine-1-phosphate, has been shown to play an important role in the migration, proliferation, and survival of several types of cell including endothelial cells. Given that S1P(1) signaling could serve as a therapeutic target, we evaluate the expression o...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-008-9193-y
更新日期：2008-10-01 00:00:00
abstract：:The aim of the present study was to investigate the association between KDM3A expression and the clinicopathological parameters of patients with epithelial ovarian cancer (EOC). KDM3A expression was analyzed using immunohistochemistry in EOC tissue microarray containing 90 paired cases of EOC and matched normal contro...
journal_title：Journal of molecular histology
pub_type： 撤回出版物,收录出版
doi：10.1007/s10735-015-9642-3
更新日期：2016-04-01 00:00:00
abstract：:Vesicular monoamine transporter 2 (VMAT2) is expressed in pancreatic beta cells and has recently been proposed as a target for measurement of beta cell mass in vivo. We questioned, (1) What proportion of beta cells express VMAT2? (2) Is VMAT2 expressed by other pancreatic endocrine or non-endocrine cells? (3) Is the r...
journal_title：Journal of molecular histology
pub_type： 杂志文章,多中心研究
doi：10.1007/s10735-008-9195-9
更新日期：2008-10-01 00:00:00
abstract：:Inpp5f-v3 is a transcriptional variant of Inpp5f (inositol polyphosphate-5-phosphatase F) and locates in distal mouse chromosome 7. It is a paternally expressed imprinted gene in mouse. In this study, we examined the spatiotemporal patterns of Inpp5f-v3 gene during the mouse development. The northern blotting analysis...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-011-9321-y
更新日期：2011-04-01 00:00:00
abstract：:Chronic stress is regarded as a significant factor in the etiology of the many diseases. Numerous methods have been developed through which the effect of chronic stress is examined. The aim of this study is to demonstrate the new experimental model for analysis of immuno-suppression induced by chronic restraint stress...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09886-5
更新日期：2020-08-01 00:00:00
abstract：:Computational modelling of whole biological systems from cells to organs is gaining momentum in cell biology and disease studies. This pathway is essential for the derivation of explanatory frameworks that will facilitate the development of a predictive capacity for estimating outcomes or risk associated with particul...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-004-2653-0
更新日期：2004-09-01 00:00:00
abstract：:TGF-β1, SNAI1 and MMP-9 are implicated in tumor invasion and metastasis. The purpose of this study was to examine TGF-β1, SNAI1 and MMP-9 expression in papillary thyroid carcinoma (PTC), and to assess association of TGF-β1, SNAI1 and MMP-9 expression with several clinicopathological indicators of PTC. TGF-β1, SNAI1 an...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9557-9
更新日期：2014-08-01 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) is an important angiogenic factor in the pituitary gland. The objective of this study was to unveil the VEGF subcellular localisation in different pituitary cell types and to evaluate changes in its expression at different time intervals after oestrogen stimulation. A relevant...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-005-9012-7
更新日期：2005-10-01 00:00:00
abstract：:Spinal cord injury frequently results in permanent loss of neurological function. It includes many complex molecular and biochemical mechanisms. G-protein-coupled receptor kinase 6 (GRK6) is an intracellular kinase that regulates the sensitivity of certain G-protein-coupled receptors. Some studies reported GRK2 and GR...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9486-7
更新日期：2013-06-01 00:00:00
abstract：:Microscopy is advancing at a rapid pace, enabling high-speed, high-resolution analyses to be conducted in a wide range of cellular contexts. For example, the capacity to quickly capture high-resolution images from multiple optical sections over multiple channels with confocal microscopy has allowed researchers to gain...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09850-y
更新日期：2019-12-01 00:00:00
abstract：:The natriuretic peptide (NP) system comprises of three ligands, the Atrial Natriuretic Peptide (ANP), Brain Natriuretic peptide (BNP) and C-type Natriuretic peptide (CNP), and three natriuretic peptide receptors, NPRA, NPRB and NPRC. Here we present a comprehensive study of the natriuretic peptide system in healthy mu...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09849-5
更新日期：2020-02-01 00:00:00
