Abstract:
:The aim of this study was to investigate oxidative DNA damage during 4-nitroquinoline 1-oxide (4NQO)-induced rat tongue carcinogenesis. For this purpose, male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12, and 20 weeks. Ten animals were used as negative control. The alkaline Comet assay modified with lesion-specific enzymes was used to detect single and double strand breaks, labile sites (SBs), and oxidised purines and pyrimidines. Although no histopathological abnormalities were induced in the epithelium after 4 weeks of carcinogen exposure, oxidative DNA damage was detected in the 'normal' oral epithelium. In pre-neoplastic lesions and squamous cell carcinomas induced after 12 and 20 weeks following carcinogen exposure, respectively, oxidative DNA damage was also increased (P < 0.05) when compared to negative control. In conclusion, our results suggest that oxidative DNA damage is an early event during multistep carcinogenesis assay induced by 4NQO. This kind of approach should be considered to persons with high risk of oral cancer, such as in smokers or alcohol consumers.
journal_name
J Mol Histoljournal_title
Journal of molecular histologyauthors
Miranda SR,Noguti J,Carvalho JG,Oshima CT,Ribeiro DAdoi
10.1007/s10735-011-9323-9subject
Has Abstractpub_date
2011-04-01 00:00:00pages
181-6issue
2eissn
1567-2379issn
1567-2387journal_volume
42pub_type
杂志文章abstract::The distribution of molecular components of interendothelial tight junctions (TJs) was studied in rat blood-brain barrier (BBB) microvessels, using immunogold cytochemistry applied to electron microscopy. Samples of rat brains, both normal (unaffected) and osmotically-affected (1, 5, and 30 min after intracarotid infu...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/10.1007/s10735-004-1318-3
更新日期:2004-06-01 00:00:00
abstract::Previous findings have shown that P2X-purinoceptor-mediated signaling pathways regulate the release of ACh in the retina. We previously reported the existence of immunoreactivity for P2X1-, P2X2-, P2X4-, and P2X7-purinoceptors in mouse retina and speculated that P2X2 and P2X7-purinoceptors may modulate the activity of...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-007-9107-4
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abstract::Gekko japonicus undergoes dramatic changes in the caudal spinal cord after tail amputation. The amputation induces cell proliferation in the caudal ependymal tube. We performed hematoxylin and eosin staining at different time points in the regeneration process to investigate the morphological characterization of the r...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-012-9466-3
更新日期:2013-06-01 00:00:00
abstract::The aim of this study was to ascertain whether, like many cell types in cartilaginous tissues if type XI collagen was a pericellular component of annulus fibrosus (AF) cells and chondrocytes. Fine fibrillar networks were visualised which were perlecan, HS (MAb 10E4) and type XI collagen positive. Heparitinase-III pre-...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-019-09823-1
更新日期:2019-06-01 00:00:00
abstract::Nickel nanoparticles (Ni NPs) have been applied in various fields along with the rapid development of nanotechnology. However, the potential adverse health effects of the Ni NPs are unclear. To investigate the cyto- and genotoxicity and compare the differences between the Ni NPs and the nickel fine particles (Ni FPs),...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-016-9671-6
更新日期:2016-06-01 00:00:00
abstract::DLBCL is the most common lymphoma subtype occurring in older populations as well as in younger HIV infected patients. The current treatment options for DLBCL are effective for most patients yet the relapse rate is high. While many biomarkers for DLBCL exist, they are not in clinical use due to low sensitivity and spec...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-016-9695-y
更新日期:2016-12-01 00:00:00
abstract::S100A8 and S100A9 are calcium-binding proteins expressed in myeloid cells and are markers of numerous inflammatory diseases in humans. S100A9 has been associated with dystrophic calcification in human atherosclerosis. Here we demonstrate S100A8 and S100A9 expression in murine and human bone and cartilage cells. Only S...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-007-9117-2
更新日期:2007-10-01 00:00:00
abstract::Craniofacial autologous bone grafts offer superior outcomes to long bone grafts in the reconstruction of maxillofacial bone defects, but the mechanism responsible for this superiority has not yet been illustrated clearly. Osteoblasts play vital roles in bone development and regeneration. However, presently, only a few...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-019-09810-6
更新日期:2019-04-01 00:00:00
abstract::Tissue microarrays have become an essential tool in translational pathology. They are used to confirm results from other experimental platforms, such as expression microarrays, as well as a primary tool to explore the expression profile of proteins by immunohistochemical analysis. Tissue microarrays are routinely used...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-006-9049-2
更新日期:2007-05-01 00:00:00
abstract::Low muscle strength is observed during the peri-and postmenopausal periods, when the secretion of ovarian hormones is drastically reduced. It is also a predictive of adverse health events as well as incident mobility limitation and disability. The objective of the present study is to study the biochemical and the hist...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-011-9326-6
更新日期:2011-06-01 00:00:00
abstract::Heparan sulfate proteoglycans (HSPGs) are complex and labile macromolecular moieties on the surfaces of cells that control the activities of a range of extracellular proteins, particularly those driving growth and regeneration. Here, we examine the biosynthesis of heparan sulfate (HS) sugars produced by cultured MC3T3...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-007-9123-4
更新日期:2007-10-01 00:00:00
abstract::This study aimed to explore whether the altered expression of tumor necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF) and apoptotic changes in mid zone (MZ) and rupture zone (RZ) of fetal membranes (FM) are regulatory mechanisms associated with labor at term. Fifteen FM specimens were collected ...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-013-9553-0
更新日期:2014-06-01 00:00:00
abstract::Aquaporins (AQPs), the rapid transition pores for water molecules, play an important role in maintenance of intracellular water balance. Studies showed that AQPs were also involved in occurrence, development, invasion and metastasis of tumors. In this study, we aimed to explore the distribution and expression differen...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-016-9657-4
更新日期:2016-04-01 00:00:00
abstract::TGF-β1, SNAI1 and MMP-9 are implicated in tumor invasion and metastasis. The purpose of this study was to examine TGF-β1, SNAI1 and MMP-9 expression in papillary thyroid carcinoma (PTC), and to assess association of TGF-β1, SNAI1 and MMP-9 expression with several clinicopathological indicators of PTC. TGF-β1, SNAI1 an...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-013-9557-9
更新日期:2014-08-01 00:00:00
abstract::Five subtypes of dopamine receptor exist in two subfamilies: two D(1)-like (D(1) and D(5)) and three D(2)-like (D(2), D(3) and D(4)). We produced novel monoclonal antibodies against all three D(2)-like receptors and used them to localize receptors in Ntera-2 (NT-2) cells, the human neuronal precursor cell line. Most o...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-007-9106-5
更新日期:2007-08-01 00:00:00
abstract::In order to study the presence of sulphated glycoconjugates in the first mineralised layer juxtaposed to the root dentine (the hyaline layer), we have examined the early stages of molar root development by ultrastructural cytochemistry using Cuprolinic Blue combined with enzymatic pretreatment. Upper molars from 10 to...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1023/b:hijo.0000021089.75408.7c
更新日期:2004-01-01 00:00:00
abstract::Osteoprotegerin (OPG) and the receptor activator of nuclear factor (NF)-kB ligand (RANKL) are key regulators of osteoclastogenesis. The present study had the main aim of showing the localization of OPG and RANKL mRNA and protein in serial sections of the rat femurs and tibiae by immunohistochemistry (IHC) and in situ ...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-004-3839-1
更新日期:2005-02-01 00:00:00
abstract::Follistatin like-1 (Fstl1) is a secreted glycoprotein and can be up-regulated by TGF-β1. To better study the function of Fstl1 in lung development, we examined Fstl1 expression in the developing lung, in a cell type specific manner, using a tamoxifen inducible Fstl1-reporter mouse strain. Our results show that Fstl1 i...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-018-9780-5
更新日期:2018-08-01 00:00:00
abstract::Recent studies have shown that chronic endothelial dysfunction can impair multiple aspects of renal physiology and, in turn, contribute to renal fibrosis. Sphingosine 1-phosphate (S1P) has been highlighted as an endothelial barrier-stabilizing mediator. The aim of our study was to investigate the effect of FTY720, an ...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-013-9521-8
更新日期:2013-12-01 00:00:00
abstract::The aim of the present study was to investigate the association between KDM3A expression and the clinicopathological parameters of patients with epithelial ovarian cancer (EOC). KDM3A expression was analyzed using immunohistochemistry in EOC tissue microarray containing 90 paired cases of EOC and matched normal contro...
journal_title:Journal of molecular histology
pub_type: 撤回出版物,收录出版
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更新日期:2016-04-01 00:00:00
abstract::Soluble epoxide hydrolase (sEH) is a bifunctional enzyme with a C-terminal epoxide hydrolase activity and an N-terminal phosphatase activity. Arachidonic acid epoxides, previously suggested to be involved in apoptosis, oncogenesis and cell proliferation, are generated by cytochrome P450 epoxygenases and are good subst...
journal_title:Journal of molecular histology
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doi:10.1007/s10735-006-9050-9
更新日期:2006-05-01 00:00:00
abstract::MT1-MMP and TIMP-2 are well known for their roles in remodelling of extracellular matrix components. However, reports are emerging on the involvement of these molecules in cell kinetics. In the rat incisor tooth, a shortening treatment increases the eruption and cell proliferation rates. However, the role of MT1-MMP a...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-010-9295-1
更新日期:2010-12-01 00:00:00
abstract::Spinal cord injury frequently results in permanent loss of neurological function. It includes many complex molecular and biochemical mechanisms. G-protein-coupled receptor kinase 6 (GRK6) is an intracellular kinase that regulates the sensitivity of certain G-protein-coupled receptors. Some studies reported GRK2 and GR...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-013-9486-7
更新日期:2013-06-01 00:00:00
abstract::During human pregnancy the placenta produces a variety of proteins like steroid hormones and their receptors that are responsible for the establishment and ongoing of the feto-placental unit. Inhibins are dimeric glycoproteins, composed of an alpha-subunit and one of two possible beta-subunits (beta (A) or beta (B)). ...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-006-9029-6
更新日期:2006-01-01 00:00:00
abstract::The goal of this study was to investigate whether sub-chronic anti-oxidant treatment with ascorbic acid (Vit C) is able to protect the heart against myocardial infarction. The effects of Vit C treatment on the histopatological changes and immunohistochemistry for p53, COX-2 and iNOS were evaluated in rats submitted to...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-009-9218-1
更新日期:2009-04-01 00:00:00
abstract::Dentin is a major component of teeth that protects dental pulp and maintains tooth health. Bone morphogenetic protein (BMP) signaling is required for the formation of dentin. Mice lacking a BMP type I receptor, activin A receptor type 1 (ACVR1), in the neural crest display a deformed mandible. Acvr1 is known to be exp...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-018-9806-z
更新日期:2019-02-01 00:00:00
abstract::In the last few decades, several growth factors were identified in the testis of various mammalian species. Growth factors are shown to promote cell proliferation, regulate tissue differentiation, and modulate organogenesis. In the present investigation we have studied the localization of EGF and EGFR in the adult bov...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-007-9089-2
更新日期:2007-06-01 00:00:00
abstract::Elevated expression of CXCL9 has been shown to involve in the infiltration of inflammatory cells and liver damage after Hepatitis B virus (HBV) infection. However, whether and by what underlying mechanism does CXCL9 play a role in HBV infection associated hepatocellular carcinoma (HCC) invasion ability remain unclear....
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-014-9593-0
更新日期:2014-12-01 00:00:00
abstract::Polycystic ovary syndrome (PCOS) is a major health problem in reproductive-aged women worldwide, but the precise pathogenesis of PCOS remains unclear. Our previous study revealed that hypoxia-inducible factor (HIF)-1a mediated endothelin (ET)-2 signaling plays an important role in ovulation in rats. Therefore, the pre...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-015-9609-4
更新日期:2015-04-01 00:00:00
abstract::Multidrug resistance-associated protein 1 (MRP1) belongs to ATP-binding cassette transporters family. The overexpression of MRP1 is predominantly related with the failure of chemo-radiotherapy in various tumors. However, its possible role in hypertrophic scar (HS) is hardly investigated. Here we showed that the mRNA l...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-015-9629-0
更新日期:2015-10-01 00:00:00