Abstract:
:Five subtypes of dopamine receptor exist in two subfamilies: two D(1)-like (D(1) and D(5)) and three D(2)-like (D(2), D(3) and D(4)). We produced novel monoclonal antibodies against all three D(2)-like receptors and used them to localize receptors in Ntera-2 (NT-2) cells, the human neuronal precursor cell line. Most of the immunostaining for all three receptors colocalized with mannose-6-phosphate receptor, a marker for late endosomes formed by internalization of the plasma membrane. This result was obtained with antibodies against three different epitopes on the D(3) receptor, to rule out the possibility of cross-reaction with another protein, and controls without primary antibody or in the presence of competitor antigen were completely negative. In rat cerebral cortex and hippocampus, some of the dopamine receptor staining was found in similar structures in neuronal cell cytoplasm. Only some of the neurons were positive for dopamine receptors and the pattern was consistent with previously-reported patterns of innervation by dopamine-producing neurons. Endosomal dopamine receptors may provide a useful method for identifying cell bodies of dopamine-responsive neurons to complement methods that detect only active receptors in the neuronal cell membrane.
journal_name
J Mol Histoljournal_title
Journal of molecular histologyauthors
Wolstencroft EC,Simic G,thi Man N,Holt I,Lam le T,Buckland PR,Morris GEdoi
10.1007/s10735-007-9106-5subject
Has Abstractpub_date
2007-08-01 00:00:00pages
333-40issue
4eissn
1567-2379issn
1567-2387journal_volume
38pub_type
杂志文章abstract::p21-activated Kinase 6 (PAK6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. PAK kinases are well-known regulators of a wide variety of cellular functions, including regulation of cytoskeleton rearrangement, cell survival, apoptosis and the mitogen-activated protein kinase signaling p...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-011-9324-8
更新日期:2011-06-01 00:00:00
abstract::Polycystic ovary syndrome (PCOS) is a major health problem in reproductive-aged women worldwide, but the precise pathogenesis of PCOS remains unclear. Our previous study revealed that hypoxia-inducible factor (HIF)-1a mediated endothelin (ET)-2 signaling plays an important role in ovulation in rats. Therefore, the pre...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-015-9609-4
更新日期:2015-04-01 00:00:00
abstract::Nel-like molecule-1 (NELL-1) is a novel highly specific growth factor that can induce osteoblast differentiation and bone formation as well as odontoblast differentiation. Recent studies have suggested that NELL-1 can synergistically increase bone formation and regeneration with bone morphogenetic protein 2 (BMP2) and...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-019-09822-2
更新日期:2019-06-01 00:00:00
abstract:AIM:To compare the expression of p57 as indirect marker of genomic imprinting of CDKN1C in a series of infantile hemangiomas (IH) of patients with and without Beckwith-Wiedemann syndrome. MATERIALS AND METHODS:Cases of mammary, salivary gland, liver (one each), and placental (2 cases) capillary hemangiomas all with hi...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-005-2255-5
更新日期:2005-03-01 00:00:00
abstract::Histochemical and morphological research increasingly relies upon quantification of complex biological systems. For such investigations to be meaningful, quantification techniques must meet the seemingly conflicting requirements of being theoretically robust, yet sufficiently practical to facilitate widespread applica...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-004-2187-5
更新日期:2004-08-01 00:00:00
abstract::p53 plays a pivotal role in the prevention of human tumor formation. p73 and p63 are new members of the p53 tumor suppressor family, which are becoming increasingly recognized as important players in human tumorigenesis. However, the roles of these proteins are not well elucidated in extrahepatic bile duct (EBD) carci...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-007-9084-7
更新日期:2007-06-01 00:00:00
abstract::Human pluripotent stem cell (hPSC)-derived cardiomyocytes (CMs) hold great promise for the repair of the injured heart, but optimal cell production in a fully chemically defined and cost-effective system is essential for the efficacy and safety of cell transplantation therapies. In this study, we provided a simple and...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-020-09926-0
更新日期:2021-02-01 00:00:00
abstract::Selective cyclooxygenase-2 non-steroidal anti-inflammatory drugs are known to inhibit bone repair, especially when long-term administration is required due to chronicle inflammatory diseases. In order to evaluate the action of this drug in bone repair during short-term administration, 48 rats underwent surgical bone d...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-008-9176-z
更新日期:2008-08-01 00:00:00
abstract::Low muscle strength is observed during the peri-and postmenopausal periods, when the secretion of ovarian hormones is drastically reduced. It is also a predictive of adverse health events as well as incident mobility limitation and disability. The objective of the present study is to study the biochemical and the hist...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-011-9326-6
更新日期:2011-06-01 00:00:00
abstract::Odontogenic keratocysts (OKCs) are jaw cystic lesions which are characterized by local invasion and high recurrence rate. The majority of OKCs are exposed to microorganisms and occur along with focal inflammatory infiltrates. Cyst fluids are biological fluids that contain a large content of cytokines and immune globul...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-019-09829-9
更新日期:2019-08-01 00:00:00
abstract::Sphingosine-1-phosphate receptor 1 (S1P(1)), a receptor for sphingosine-1-phosphate, has been shown to play an important role in the migration, proliferation, and survival of several types of cell including endothelial cells. Given that S1P(1) signaling could serve as a therapeutic target, we evaluate the expression o...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-008-9193-y
更新日期:2008-10-01 00:00:00
abstract::Adipose-derived mesenchymal stem cell (ADSC) with a high capacity of chondrogenic differentiation was a promising candidate for cartilage defect treatment. This study's objective is to study the roles of integrin β1 (ITGB1) in regulating ADSC chondrogenic differentiations as well as the underlying mechanisms. The iden...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-020-09918-0
更新日期:2020-12-01 00:00:00
abstract::Cyclin G1 plays an essential role in the development of human carcinoma. Here, we characterized the clinical significance of Cyclin G1 and investigated its role in cellular proliferation and apoptosis of epithelial ovarian cancer (EOC). Western blot was used to evaluate the expression of Cyclin G1 in nine fresh EOC ti...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-015-9622-7
更新日期:2015-06-01 00:00:00
abstract::Chronic stress is regarded as a significant factor in the etiology of the many diseases. Numerous methods have been developed through which the effect of chronic stress is examined. The aim of this study is to demonstrate the new experimental model for analysis of immuno-suppression induced by chronic restraint stress...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-020-09886-5
更新日期:2020-08-01 00:00:00
abstract::In in vitro and in vivo systems, understanding localization and the functional role of ATP is essential, but effective methods to monitor ATP in cells and tissues are limited. Although quinacrine dihydrochloride is a well-known fluorescent dye used to detect ATP, it is limited in its use because it shows non-specific ...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-012-9474-3
更新日期:2013-04-01 00:00:00
abstract::Wnt signaling plays important roles in skeletal development. However, the activation and function of canonical Wnt signaling in joint development remains unclear. We analyzed the lineage identity and developmental changes of the Wnt-responsive cells during synovial joint formation as well as adulthood in the Wnt signa...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-009-9242-1
更新日期:2009-08-01 00:00:00
abstract::Submandibular gland (SMG) autotransplantation is an effective therapy for treating severe dry eye syndrome. However, epiphora occurs in more than 40% of patients 6 months after operation. We previously found that muscarinic acetylcholine receptor (mAChR) plays a crucial role in regulating SMG secretion partially throu...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-016-9709-9
更新日期:2017-04-01 00:00:00
abstract::Satellite glial cells (SGCs), a peripheral neuroglial cell, surround neurons and form a complete envelope around individual sensory neurons in the trigeminal ganglia (TG), which may be involved in modulating neurons in inflammation. The purpose of this study was to determine the effect of dental injury and inflammatio...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-018-9765-4
更新日期:2018-06-01 00:00:00
abstract::The aim of this study was to ascertain whether, like many cell types in cartilaginous tissues if type XI collagen was a pericellular component of annulus fibrosus (AF) cells and chondrocytes. Fine fibrillar networks were visualised which were perlecan, HS (MAb 10E4) and type XI collagen positive. Heparitinase-III pre-...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-019-09823-1
更新日期:2019-06-01 00:00:00
abstract::Heparan sulfate proteoglycans (HSPGs) are complex and labile macromolecular moieties on the surfaces of cells that control the activities of a range of extracellular proteins, particularly those driving growth and regeneration. Here, we examine the biosynthesis of heparan sulfate (HS) sugars produced by cultured MC3T3...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-007-9123-4
更新日期:2007-10-01 00:00:00
abstract::Spinal cord injury frequently results in permanent loss of neurological function. It includes many complex molecular and biochemical mechanisms. G-protein-coupled receptor kinase 6 (GRK6) is an intracellular kinase that regulates the sensitivity of certain G-protein-coupled receptors. Some studies reported GRK2 and GR...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-013-9486-7
更新日期:2013-06-01 00:00:00
abstract::Microscopy is advancing at a rapid pace, enabling high-speed, high-resolution analyses to be conducted in a wide range of cellular contexts. For example, the capacity to quickly capture high-resolution images from multiple optical sections over multiple channels with confocal microscopy has allowed researchers to gain...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-019-09850-y
更新日期:2019-12-01 00:00:00
abstract::During transmyocardial revascularization, cellular destruction of cardiomyocytes occurs as a result of the high-energy laser. However, the features of myocardial cellular destruction are unclear. The present study was undertaken to examine the structural characteristics of cell death in the myocardium following transm...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-005-5343-7
更新日期:2005-05-01 00:00:00
abstract::Epidermal adult stem cells (EpiASCs) have the potential for unlimited proliferation and differentiation, however, the ability of these stem cells to activate corneal genetic programs in response to corneal stroma stimulation needs to be further validated. Herein, a feasible strategy was developed to reconstruct the da...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-020-09879-4
更新日期:2020-06-01 00:00:00
abstract::The accurately establishment of the eruptive pathway is of vital importance. The mechanisms governing tooth eruption pathway remain little known. This study is to elucidate the roles of Semaphorin 3A (Sema 3A) in mouse tooth eruptive pathway. C57BL/6 mice (11-13 and 15-17 days after birth) were chosen to observe erupt...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-019-09838-8
更新日期:2019-10-01 00:00:00
abstract::In order to study the presence of sulphated glycoconjugates in the first mineralised layer juxtaposed to the root dentine (the hyaline layer), we have examined the early stages of molar root development by ultrastructural cytochemistry using Cuprolinic Blue combined with enzymatic pretreatment. Upper molars from 10 to...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1023/b:hijo.0000021089.75408.7c
更新日期:2004-01-01 00:00:00
abstract::MI-ER1 is a novel transcriptional regulator that plays a critical role in embryonic development and is differentially expressed in breast carcinoma. The MI-ER1 protein sequence is highly conserved among species, with 95% identity between mouse and humans and 72% between Xenopus and mouse. There are two major protein i...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-007-9116-3
更新日期:2008-02-01 00:00:00
abstract::Under normal homeostasis, melanocyte growth and behaviour is tightly controlled by the surrounding keratinocytes. Keratinocytes regulate melanocyte behaviour through a complex system of paracrine growth factors and cell-cell adhesion molecules. Pathological changes, leading to development of malignant melanoma, upset ...
journal_title:Journal of molecular histology
pub_type: 杂志文章,评审
doi:10.1023/b:hijo.0000032362.35354.bb
更新日期:2004-03-01 00:00:00
abstract::Nickel nanoparticles (Ni NPs) have been applied in various fields along with the rapid development of nanotechnology. However, the potential adverse health effects of the Ni NPs are unclear. To investigate the cyto- and genotoxicity and compare the differences between the Ni NPs and the nickel fine particles (Ni FPs),...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-016-9671-6
更新日期:2016-06-01 00:00:00
abstract::Elevated expression of CXCL9 has been shown to involve in the infiltration of inflammatory cells and liver damage after Hepatitis B virus (HBV) infection. However, whether and by what underlying mechanism does CXCL9 play a role in HBV infection associated hepatocellular carcinoma (HCC) invasion ability remain unclear....
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-014-9593-0
更新日期:2014-12-01 00:00:00