Abstract:
:TGF-β1, SNAI1 and MMP-9 are implicated in tumor invasion and metastasis. The purpose of this study was to examine TGF-β1, SNAI1 and MMP-9 expression in papillary thyroid carcinoma (PTC), and to assess association of TGF-β1, SNAI1 and MMP-9 expression with several clinicopathological indicators of PTC. TGF-β1, SNAI1 and MMP-9 protein expression in 83 PTCs and their matched normal thyroid specimens were analyzed using immunohistochemistry. The mRNA expression levels of TGF-β1, SNAI1 and MMP-9 in 12 fresh PTC specimens with lymph node metastasis (LNM), 12 fresh PTC specimens without LNM and their matched normal thyroid specimens were assessed by real-time RT-PCR. The results showed that the mRNA and protein expression levels of TGF-β1, SNAI1 and MMP-9 were significantly higher in PTCs than in their matched normal thyroid tissues. There were not significant differences in TGF-β1, SNAI1 and MMP-9 protein expression relative to age, gender, tumor size and TNM stage, except for MMP-9 whose protein expression correlated with tumor size. However, high mRNA and protein expression levels of TGF-β1, SNAI1 and MMP-9 were significantly correlated with LNM. Furthermore, TGF-β1, SNAI1 and MMP-9 protein expression were significantly correlated with one another. Concomitant expression of any two or all of the three molecules had stronger correlation with LNM than did each alone. Collectively, the present results indicate that immunohistochemical and real-time RT-PCR evaluation of TGF-β1, SNAI1 and MMP-9 expression in PTC may be useful to predict the risk of LNM in PTC patients.
journal_name
J Mol Histoljournal_title
Journal of molecular histologyauthors
Wang N,Jiang R,Yang JY,Tang C,Yang L,Xu M,Jiang QF,Liu ZMdoi
10.1007/s10735-013-9557-9subject
Has Abstractpub_date
2014-08-01 00:00:00pages
391-9issue
4eissn
1567-2379issn
1567-2387journal_volume
45pub_type
杂志文章abstract::Four and a half LIM domains 2 (FHL2) is a protein of 279 amino acids in length containing four full LIM-domains and a half LIM-domain at the amino terminus. FHL2 is one transcriptional cofactor that can interact with many different proteins, such as AP-1, BRCA1, IGFBP, and integrin, and involved in organ differentiati...
journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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更新日期:2014-12-01 00:00:00
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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abstract::Quercetin (3,5,7,3',4'-pentahydroxyflavone) is one of the most abundant natural flavonoids. It is present in various common vegetables and fruits. In this report, we examined the effect of quercetin on melanogenesis using a three-dimensional reconstituted human epidermal culture model, MelanoDerm, which is a new comme...
journal_title:Journal of molecular histology
pub_type: 杂志文章
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
pub_type: 杂志文章
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journal_title:Journal of molecular histology
pub_type: 杂志文章
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journal_title:Journal of molecular histology
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pub_type: 撤回出版物,收录出版
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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