Abstract:
:Epidermal adult stem cells (EpiASCs) have the potential for unlimited proliferation and differentiation, however, the ability of these stem cells to activate corneal genetic programs in response to corneal stroma stimulation needs to be further validated. Herein, a feasible strategy was developed to reconstruct the damaged corneal surface in a goat model with total limbal stem cell deficiency (LSCD) by transplanting EpiASCs, which had been explanted and cultured from the skin of an adult ram goat and were then purified by selecting single cell-derived clones and cultivating them on a denuded human amniotic membrane (HAM). These artificial tissues were then successfully transplanted into ewe goats with total LSCD. Binding of EpiASCs to the base membrane of an EpiASCs-HAM-Sheet (EHS) indicated their proliferating status. After transplantation, the EpiASCs could survive in the host tissue and they reconstructed the damaged ocular surface of total LSCD. The crystal reconstructed corneal epithelium expressed CK3 and Pax-6 similar to normal corneal epithelium and expressed the Sry gene after transplantation. These results demonstrated that EpiASCs could be induced to differentiate into corneal epithelial cell types in a corneal microenvironment and had the ability to activate corneal genetic programs. This work offer a foundation for promoting tissue-engineered cornea into clinical application.
journal_name
J Mol Histoljournal_title
Journal of molecular histologyauthors
Zhang P,Ma XY,Huang DT,Yang XYdoi
10.1007/s10735-020-09879-4subject
Has Abstractpub_date
2020-06-01 00:00:00pages
277-286issue
3eissn
1567-2379issn
1567-2387pii
10.1007/s10735-020-09879-4journal_volume
51pub_type
杂志文章abstract::S100A8 and S100A9 are calcium-binding proteins expressed in myeloid cells and are markers of numerous inflammatory diseases in humans. S100A9 has been associated with dystrophic calcification in human atherosclerosis. Here we demonstrate S100A8 and S100A9 expression in murine and human bone and cartilage cells. Only S...
journal_title:Journal of molecular histology
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abstract::Bone marrow-derived mesenchymal stem cells (BMMSCs) transplantation has shown to be effective in treating chronic kidney disease. However, the effectiveness of this strategy is constrained by low homing and survival rate of transplanted cells in the injured organs. Therefore, developing strategies to improve homing an...
journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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doi:10.1007/s10735-016-9679-y
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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doi:10.1007/s10735-014-9592-1
更新日期:2014-12-01 00:00:00
abstract::Adult neurogenesis is a characteristic feature of the olfactory pathways of decapod crustaceans. In crayfish and clawed lobsters, adult-born neurons are the progeny of precursor cells with glial characteristics located in a neurogenic niche on the ventral surface of the brain. The daughters of these precursor cells mi...
journal_title:Journal of molecular histology
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更新日期:2007-12-01 00:00:00
abstract::Gekko japonicus undergoes dramatic changes in the caudal spinal cord after tail amputation. The amputation induces cell proliferation in the caudal ependymal tube. We performed hematoxylin and eosin staining at different time points in the regeneration process to investigate the morphological characterization of the r...
journal_title:Journal of molecular histology
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doi:10.1007/s10735-012-9466-3
更新日期:2013-06-01 00:00:00
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journal_title:Journal of molecular histology
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更新日期:2005-10-01 00:00:00
abstract::Hyperglycemia is associated with impairment of heart function. The current study aimed to investigate the ameliorative effect of polydatin-loaded chitosan nanoparticles (PD-CSNPs), polydatin (PD) and metformin (MET) on diabetic cardiomyopathy in rats. Rats divided into six groups; normal-control, diabetic-control, dia...
journal_title:Journal of molecular histology
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doi:10.1007/s10735-020-09930-4
更新日期:2021-01-03 00:00:00
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journal_title:Journal of molecular histology
pub_type: 杂志文章
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更新日期:2004-05-01 00:00:00
abstract::Odontogenic keratocysts (OKCs) are jaw cystic lesions which are characterized by local invasion and high recurrence rate. The majority of OKCs are exposed to microorganisms and occur along with focal inflammatory infiltrates. Cyst fluids are biological fluids that contain a large content of cytokines and immune globul...
journal_title:Journal of molecular histology
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更新日期:2019-08-01 00:00:00
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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doi:10.1007/s10735-016-9657-4
更新日期:2016-04-01 00:00:00
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journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-008-9193-y
更新日期:2008-10-01 00:00:00
abstract::Microscopy is advancing at a rapid pace, enabling high-speed, high-resolution analyses to be conducted in a wide range of cellular contexts. For example, the capacity to quickly capture high-resolution images from multiple optical sections over multiple channels with confocal microscopy has allowed researchers to gain...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-019-09850-y
更新日期:2019-12-01 00:00:00
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journal_title:Journal of molecular histology
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doi:10.1007/s10735-008-9173-2
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
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journal_title:Journal of molecular histology
pub_type: 撤回出版物,收录出版
doi:10.1007/s10735-015-9642-3
更新日期:2016-04-01 00:00:00
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journal_title:Journal of molecular histology
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doi:10.1007/s10735-013-9489-4
更新日期:2013-08-01 00:00:00
abstract::MT1-MMP and TIMP-2 are well known for their roles in remodelling of extracellular matrix components. However, reports are emerging on the involvement of these molecules in cell kinetics. In the rat incisor tooth, a shortening treatment increases the eruption and cell proliferation rates. However, the role of MT1-MMP a...
journal_title:Journal of molecular histology
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doi:10.1007/s10735-010-9295-1
更新日期:2010-12-01 00:00:00
abstract::Inpp5f-v3 is a transcriptional variant of Inpp5f (inositol polyphosphate-5-phosphatase F) and locates in distal mouse chromosome 7. It is a paternally expressed imprinted gene in mouse. In this study, we examined the spatiotemporal patterns of Inpp5f-v3 gene during the mouse development. The northern blotting analysis...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-011-9321-y
更新日期:2011-04-01 00:00:00
abstract:AIM:To compare the expression of p57 as indirect marker of genomic imprinting of CDKN1C in a series of infantile hemangiomas (IH) of patients with and without Beckwith-Wiedemann syndrome. MATERIALS AND METHODS:Cases of mammary, salivary gland, liver (one each), and placental (2 cases) capillary hemangiomas all with hi...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-005-2255-5
更新日期:2005-03-01 00:00:00
abstract::The neurovascular structures in the cranial dura mater have been studied with various histological techniques in the past years. In order to obtain a proper approach to reveal the detailed structures, different labeling methods for the cranial vessels and nerve fibers were tested in this study. Firstly, the labeling c...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-020-09903-7
更新日期:2020-10-01 00:00:00
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journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-005-9003-8
更新日期:2005-06-01 00:00:00
abstract::This study aimed to explore whether the altered expression of tumor necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF) and apoptotic changes in mid zone (MZ) and rupture zone (RZ) of fetal membranes (FM) are regulatory mechanisms associated with labor at term. Fifteen FM specimens were collected ...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-013-9553-0
更新日期:2014-06-01 00:00:00
abstract::Previous studies demonstrate significant roles for passive water channels (aquaporins, AQPs) in maintaining water homeostasis in cell membranes of endometrial cells during decidualisation and embryo implantation. However, there is little information regarding the role of AQPs in the human fallopian tube, specifically ...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-012-9471-6
更新日期:2013-04-01 00:00:00