当前位置： SCI文献检索 > JOURNAL OF MOLECULAR HISTOLOGY期刊下所有文献 > A simple method for the production of bacterial controls for immunohistochemistry and fluorescent in situ hybridization.

A simple method for the production of bacterial controls for immunohistochemistry and fluorescent in situ hybridization.

Abstract：

:Immunohistochemical and fluorescent in situ hybridization (FISH) assays are useful diagnostic methods for the identification of bacteria on formalin fixed paraffin embedded histological sections. To validate an anti-bacterial antibody or an oligonucleotide probe and to ensure fidelity during subsequent analyses, suitable positive and negative controls are necessary. Suspensions of fixed bacteria are often used, but ideally, these controls should be fixed, embedded and processed in the same way of tissue samples under analysis. Herein, we describe a simple method for the production of bacterial histological control samples: the sandwich. The sandwich is composed of two external layers of equine lung parenchyma and a central layer of the target bacterium. We prepared sandwiches containing Escherichia coli, Campylobacter jejuni, and Arcanobacterium pyogenes and tested them with appropriate antibodies and Eub338 FISH probe. The sandwich is an effective and simple method to prepare bacterial histological controls fixed and processed in the same way as the diagnostic tissues.

journal_name

J Mol Histol

journal_title

Journal of molecular histology

authors

Recordati C,Radaelli E,Simpson KW,Scanziani E

doi

10.1007/s10735-008-9173-2

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

459-62

issue

4

eissn

1567-2379

issn

1567-2387

journal_volume

39

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR HISTOLOGY文献大全
  • A new approach for examining the neurovascular structure with phalloidin and calcitonin gene-related peptide in the rat cranial dura mater.

    abstract：:The neurovascular structures in the cranial dura mater have been studied with various histological techniques in the past years. In order to obtain a proper approach to reveal the detailed structures, different labeling methods for the cranial vessels and nerve fibers were tested in this study. Firstly, the labeling c...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-020-09903-7

    authors： Wang J,Xu D,Cui J,Wang S,She C,Wang H,Wu S,Zhang J,Zhu B,Bai W

    更新日期：2020-10-01 00:00:00

  • Four and a half LIM domains 2 contributes to the development of human tongue squamous cell carcinoma.

    abstract：:Four and a half LIM domains 2 (FHL2) is a protein of 279 amino acids in length containing four full LIM-domains and a half LIM-domain at the amino terminus. FHL2 is one transcriptional cofactor that can interact with many different proteins, such as AP-1, BRCA1, IGFBP, and integrin, and involved in organ differentiati...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-016-9654-7

    authors： Wang Q,Wang X,Tian X,Tang R,Xu X

    更新日期：2016-04-01 00:00:00

  • Canonical Wnt signaling activity during synovial joint development.

    abstract：:Wnt signaling plays important roles in skeletal development. However, the activation and function of canonical Wnt signaling in joint development remains unclear. We analyzed the lineage identity and developmental changes of the Wnt-responsive cells during synovial joint formation as well as adulthood in the Wnt signa...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-009-9242-1

    authors： Yamagami T,Molotkov A,Zhou CJ

    更新日期：2009-08-01 00:00:00

  • Upregulation of p21-activated Kinase 6 in rat brain cortex after traumatic brain injury.

    abstract：:p21-activated Kinase 6 (PAK6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. PAK kinases are well-known regulators of a wide variety of cellular functions, including regulation of cytoskeleton rearrangement, cell survival, apoptosis and the mitogen-activated protein kinase signaling p...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-011-9324-8

    authors： Zhao W,Yang J,Shi W,Wu X,Shao B,Wu Q,Chen J,Ni L

    更新日期：2011-06-01 00:00:00

  • Expression of cytokeratin 8, vimentin, syndecan-1 and Ki-67 during human tooth development.

    abstract：:Spatio-temporal immunolocalizations of cytokeratin 8 (CK8), vimentin, syndecan-1 and Ki-67 were analyzed in ten human incisors and canine tooth germs between the 7th and 20th developmental weeks. CK8 expression was mild to moderate in the epithelial tooth parts, while it shifted from absent or mild in its mesenchymal ...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-014-9592-1

    authors： Kero D,Kalibovic Govorko D,Vukojevic K,Cubela M,Soljic V,Saraga-Babic M

    更新日期：2014-12-01 00:00:00

  • Subcellular localisation of VEGF in different pituitary cells. Changes of its expression in oestrogen induced prolactinomas.

    abstract：:Vascular endothelial growth factor (VEGF) is an important angiogenic factor in the pituitary gland. The objective of this study was to unveil the VEGF subcellular localisation in different pituitary cell types and to evaluate changes in its expression at different time intervals after oestrogen stimulation. A relevant...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-005-9012-7

    authors： Mukdsi JH,De Paul AL,Gutiérrez S,Roth FD,Aoki A,Torres AI

    更新日期：2005-10-01 00:00:00

  • Immunogold study of altered expression of some interendothelial junctional molecules in the brain blood microvessels of diabetic scrapie-infected mice.

    abstract：:Quantitative immunogold procedure was used to study the distribution of molecular components of interendothelial junctions in blood-brain barrier (BBB) microvessels of scrapie infected SJL/J hyperglycemic mice showing obesity and reduced glucose tolerance. Samples of brain (fronto-parietal cerebral cortex and thalamo-...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-006-9026-9

    authors： Vorbrodt AW,Dobrogowska DH,Tarnawski M,Meeker HC,Carp RI

    更新日期：2006-01-01 00:00:00

  • Osteogenic and angiogenic characterization of mandible and femur osteoblasts.

    abstract：:Craniofacial autologous bone grafts offer superior outcomes to long bone grafts in the reconstruction of maxillofacial bone defects, but the mechanism responsible for this superiority has not yet been illustrated clearly. Osteoblasts play vital roles in bone development and regeneration. However, presently, only a few...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-019-09810-6

    authors： Yang X,Jiang J,Zhou L,Wang S,He M,Luo K,Chen Y,Xu X

    更新日期：2019-04-01 00:00:00

  • Immunocytochemical localization of secretory component in Paneth cell secretory granules-rat Paneth cells participate in acquired immunity.

    abstract：:With the marker of Paneth cells-lysozyme, secretory component (SC) immunoreactivity was demonstrated exclusively in Paneth cells of rat small intestine. The other types of epithelial cells (columnar, goblet, endocrine) were negative. On electron microscopic level, many SC-positive colloidal gold particles were found i...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-005-9003-8

    authors： Tang QJ,Tao KZ,Yun-Liu,Sun XJ,Geng MY,Jiang CL

    更新日期：2005-06-01 00:00:00

  • Expression of inhibin/activin subunits alpha (-alpha), beta A (-beta (A)) and beta B (-beta (B)) in placental tissue of normal and intrauterine growth restricted (IUGR) pregnancies.

    abstract：:During human pregnancy the placenta produces a variety of proteins like steroid hormones and their receptors that are responsible for the establishment and ongoing of the feto-placental unit. Inhibins are dimeric glycoproteins, composed of an alpha-subunit and one of two possible beta-subunits (beta (A) or beta (B)). ...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-006-9029-6

    authors： Mylonas I,Schiessl B,Jeschke U,Vogl J,Makrigiannakis A,Kuhn C,Kunze S,Schulze S,Kainer F,Friese K

    更新日期：2006-01-01 00:00:00

  • Expression and clinical significance of the HIF-1a/ET-2 signaling pathway during the development and treatment of polycystic ovary syndrome.

    abstract：:Polycystic ovary syndrome (PCOS) is a major health problem in reproductive-aged women worldwide, but the precise pathogenesis of PCOS remains unclear. Our previous study revealed that hypoxia-inducible factor (HIF)-1a mediated endothelin (ET)-2 signaling plays an important role in ovulation in rats. Therefore, the pre...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-015-9609-4

    authors： Wang F,Zhang Z,Wang Z,Xiao K,Wang Q,Su J,Wang Z

    更新日期：2015-04-01 00:00:00

  • Effects of Nel-like molecule-1 and bone morphogenetic protein 2 combination on rat pulp repair.

    abstract：:Nel-like molecule-1 (NELL-1) is a novel highly specific growth factor that can induce osteoblast differentiation and bone formation as well as odontoblast differentiation. Recent studies have suggested that NELL-1 can synergistically increase bone formation and regeneration with bone morphogenetic protein 2 (BMP2) and...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-019-09822-2

    authors： Wu J,Wang Q,Han Q,Zhu H,Li M,Fang Y,Wang X

    更新日期：2019-06-01 00:00:00

  • FTY720 prevents progression of renal fibrosis by inhibiting renal microvasculature endothelial dysfunction in a rat model of chronic kidney disease.

    abstract：:Recent studies have shown that chronic endothelial dysfunction can impair multiple aspects of renal physiology and, in turn, contribute to renal fibrosis. Sphingosine 1-phosphate (S1P) has been highlighted as an endothelial barrier-stabilizing mediator. The aim of our study was to investigate the effect of FTY720, an ...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-013-9521-8

    authors： Ni H,Chen J,Pan M,Zhang M,Zhang J,Chen P,Liu B

    更新日期：2013-12-01 00:00:00

  • Aging up-regulates ARA55 in stromal cells, inducing androgen-mediated prostate cancer cell proliferation and migration.

    abstract：:Stromal cells in the peripheral zone (PZ) of the prostate from older males (PZ-old) could significantly promote Prostate cancer (PCa) growth compared with stromal cells from young males (PZ-young). But the mechanism is still unknown. In the co-culture system with PZ-old cells, Pc3/Du145 cells showed advanced prolifera...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-016-9679-y

    authors： Zou Q,Cui D,Liang S,Xia S,Jing Y,Han B

    更新日期：2016-06-01 00:00:00

  • Hormonal regulation of uterine natural killer cells in mouse preimplantation uterus.

    abstract：:Uterine Natural Killer (uNK) cells are the most abundant lymphocyte population recruited in the uteri during murine and human pregnancy. Previous investigation on uNK cells during mouse pregnancy focused more on its accumulation in postimplantation periods, which were believed to play important roles in regulating tro...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-010-9256-8

    authors： Kuang H,Peng H,Xu H,Zhang B,Peng J,Tan Y

    更新日期：2010-02-01 00:00:00

  • Co-expression of interleukin-6 and human growth hormone in apparently normal prostate biopsies that ultimately progress to prostate cancer using low pH, high temperature antigen retrieval.

    abstract：:Prostate cancer is the most common cancer in American men and the second leading cause of cancer deaths in this group. Both growth hormone (GH) and the inflammatory cytokine interleukin 6 (IL-6) have been implicated in prostate cancer progression. Studies in other systems have shown that an increase in GH results in a...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-006-9027-8

    authors： Slater MD,Murphy CR

    更新日期：2006-01-01 00:00:00

  • Muscarinic acetylcholine receptor-mediated tight junction opening is involved in epiphora in late phase of submandibular gland transplantation.

    abstract：:Submandibular gland (SMG) autotransplantation is an effective therapy for treating severe dry eye syndrome. However, epiphora occurs in more than 40% of patients 6 months after operation. We previously found that muscarinic acetylcholine receptor (mAChR) plays a crucial role in regulating SMG secretion partially throu...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-016-9709-9

    authors： Yang NY,Ding C,Li J,Zhang Y,Xiang RL,Wu LL,Yu GY,Cong X

    更新日期：2017-04-01 00:00:00

  • Natriuretic peptide system expression in murine and human submandibular salivary glands: a study of the spatial localisation of ANB, BNP, CNP and their receptors.

    abstract：:The natriuretic peptide (NP) system comprises of three ligands, the Atrial Natriuretic Peptide (ANP), Brain Natriuretic peptide (BNP) and C-type Natriuretic peptide (CNP), and three natriuretic peptide receptors, NPRA, NPRB and NPRC. Here we present a comprehensive study of the natriuretic peptide system in healthy mu...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-019-09849-5

    authors： Ahmed A,Gulino A,Amayo S,Arancio W,Florena AM,Belmonte B,Jurjus A,Leone A,Miletich I

    更新日期：2020-02-01 00:00:00

  • The expression of water channel proteins during human salivary gland development: a topographic study of aquaporins 1, 3 and 5.

    abstract：:Some members of aquaporin family (AQP) plays crucial functions in salivary synthesis and secretion. These proteins expression has already been reported during salivary gland formation, however no previous studies in human developing glands have been performed. We evaluated AQP1, 3 and 5 expression through the stages o...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-017-9731-6

    authors： de Paula F,Teshima THN,Hsieh R,Souza MM,Coutinho-Camillo CM,Nico MMS,Lourenco SV

    更新日期：2017-12-01 00:00:00

  • Type XI collagen-perlecan-HS interactions stabilise the pericellular matrix of annulus fibrosus cells and chondrocytes providing matrix stabilisation and homeostasis.

    abstract：:The aim of this study was to ascertain whether, like many cell types in cartilaginous tissues if type XI collagen was a pericellular component of annulus fibrosus (AF) cells and chondrocytes. Fine fibrillar networks were visualised which were perlecan, HS (MAb 10E4) and type XI collagen positive. Heparitinase-III pre-...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-019-09823-1

    authors： Smith SM,Melrose J

    更新日期：2019-06-01 00:00:00

  • Relationship between pancreatic vesicular monoamine transporter 2 (VMAT2) and insulin expression in human pancreas.

    abstract：:Vesicular monoamine transporter 2 (VMAT2) is expressed in pancreatic beta cells and has recently been proposed as a target for measurement of beta cell mass in vivo. We questioned, (1) What proportion of beta cells express VMAT2? (2) Is VMAT2 expressed by other pancreatic endocrine or non-endocrine cells? (3) Is the r...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章,多中心研究

    doi：10.1007/s10735-008-9195-9

    authors： Saisho Y,Harris PE,Butler AE,Galasso R,Gurlo T,Rizza RA,Butler PC

    更新日期：2008-10-01 00:00:00

  • Activation of satellite glial cells in trigeminal ganglion following dental injury and inflammation.

    abstract：:Satellite glial cells (SGCs), a peripheral neuroglial cell, surround neurons and form a complete envelope around individual sensory neurons in the trigeminal ganglia (TG), which may be involved in modulating neurons in inflammation. The purpose of this study was to determine the effect of dental injury and inflammatio...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-018-9765-4

    authors： Liu H,Zhao L,Gu W,Liu Q,Gao Z,Zhu X,Wu Z,He H,Huang F,Fan W

    更新日期：2018-06-01 00:00:00

  • Functional expression of ERG1 potassium channels in rat alveolar macrophages.

    abstract：:Alveolar macrophages (AMs) play a vital role in lung immunity. The recent studies demonstrated that potassium channels were associated with macrophage functions, such as activation, migration and cytokines secretion. However, less is known regarding the expression and function of ERG channels in AMs. Our study showed ...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-012-9458-3

    authors： Dong H,Ji Z,Liu M,Wang Y,Bai X,Wang T,Liu Z,Wu Y,Zhang B,Luo Y,Li Z,Dong M

    更新日期：2013-02-01 00:00:00

  • The member of high temperature requirement family HtrA2 participates in neuronal apoptosis after intracerebral hemorrhage in adult rats.

    abstract：:The members of high-temperature requirement (HtrA) family are evolutionarily conserved serine proteases that combine a trypsin-like protease domain with at least one PDZ interaction domain. HtrA2, a special one, is mainly located in mitochondria and required for maintaining homeostasis. Once released into cytoplasm, H...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-013-9489-4

    authors： Sun H,Li L,Zhou F,Zhu L,Ke K,Tan X,Xu W,Rui Y,Zheng H,Zhou Z,Yang H

    更新日期：2013-08-01 00:00:00

  • Down-regulation of SETD6 protects podocyte against high glucose and palmitic acid-induced apoptosis, and mitochondrial dysfunction via activating Nrf2-Keap1 signaling pathway in diabetic nephropathy.

    abstract：:Diabetic nephropathy (DN), a serious complication of hyperglycemia, is one of the most common causes of end-stage renal disease (ESRD). Glomerular podocyte injury is a major mechanism that leads to DN. However, the mechanisms underlying podocyte injury are ambiguous. In this study, we sought to investigate the contrib...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-020-09904-6

    authors： Wang X,Liu Q,Kong D,Long Z,Guo Y,Wang S,Liu R,Hai C

    更新日期：2020-10-01 00:00:00

  • Expression of glucose transporter 1 is associated with loss of heterozygosity of chromosome 1p in oligodendroglial tumors WHO grade II.

    abstract：:Objective Oligodendroglial tumors with a loss of heterozygosity of 1p (LOH1p) appear to have a better prognosis than oligodendrogliomas without LOH. Previously, we reported glucose uptake in low-grade oligodendroglial tumors to be related to LOH 1p status. Here, we performed an immunohistochemical study of the common ...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-008-9191-0

    authors： Stockhammer F,von Deimling A,Synowitz M,Blechschmidt C,van Landeghem FK

    更新日期：2008-10-01 00:00:00

  • CNS neurotrophins are biologically active and expressed by multiple cell types.

    abstract：:Neutrotrophins are increasingly appreciated as potential modulators of neuronal function in the adult central nervous system (CNS). To describe the neurotrophin environment within the adult CNS, mRNA and protein expression patterns of neurotrophins-3 and -4 and of brain-derived neurotrophin were investigated in adult ...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-004-0778-9

    authors： Riley CP,Cope TC,Buck CR

    更新日期：2004-11-01 00:00:00

  • ELK1 activated-long noncoding RNA LBX2-AS1 aggravates the progression of ovarian cancer through targeting miR-4784/KDM5C axis.

    abstract：:As one of the most common cancers in female, ovarian cancer (OC) has become a serious public burden now. Mounting researches have indicated long noncoding RNAs (lncRNAs) can affect many biological processes including cancer development. LncRNA LBX2-AS1 was identified to be an oncogene in some cancers, but the role of ...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-020-09921-5

    authors： Gu H,Lin R,Zheng F,Zhang Q

    更新日期：2021-02-01 00:00:00

  • The role of MYB proto-oncogene like 2 in tamoxifen resistance in breast cancer.

    abstract：:Despite the efficacy of tamoxifen in preventing disease relapse, a large portion of breast cancer patients show intrinsic or acquired resistance to tamoxifen, leading to treatment failure and unfavorable clinical outcome. MYB proto-oncogene like 2 (MYBL2) is a transcription factor implicated in the initiation and prog...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-020-09920-6

    authors： Li X,Zhang X,Wu CC,Li PP,Fu YM,Xie LH,Sun SS,Zhou YY,Zhu BL

    更新日期：2021-02-01 00:00:00

  • Localization of the epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in the bovine testis.

    abstract：:In the last few decades, several growth factors were identified in the testis of various mammalian species. Growth factors are shown to promote cell proliferation, regulate tissue differentiation, and modulate organogenesis. In the present investigation we have studied the localization of EGF and EGFR in the adult bov...

    journal_title：Journal of molecular histology

    pub_type： 杂志文章

    doi：10.1007/s10735-007-9089-2

    authors： Kassab M,Abd-Elmaksoud A,Ali MA

    更新日期：2007-06-01 00:00:00