Abstract：
:As one of the most common cancers in female, ovarian cancer (OC) has become a serious public burden now. Mounting researches have indicated long noncoding RNAs (lncRNAs) can affect many biological processes including cancer development. LncRNA LBX2-AS1 was identified to be an oncogene in some cancers, but the role of LBX2-AS1 in OC remains to be elucidated. Bioinformatics analysis and experiments including ChIP, RT-qPCR, RIP, luciferase reporter, western blot and CCK-8 were performed to explore the role of LBX2-AS1 in OC. LBX2-AS1 expression was markedly increased in OC tissues and cell lines. Functionally, LBX2-AS1 silencing inhibited cell proliferation, migration and stemness but facilitated cell apoptosis in OC. Moreover, depletion of LBX2-AS1 suppressed tumor growth of OC in vivo. Mechanically, LBX2-AS1 was activated by transcriptional factor ELK1. ELK1 enhanced the expression of LBX2-AS1 in OC cells. In addition, miR-4784 was confirmed to be sponged by LBX2-AS1. There was a negative expression correlation between LBX2-AS1 and miR-4784 in OC tissues. Subsequently, KDM5C was identified to be a direct target of miR-4784 in OC cells. KDM5C was negatively regulated by miR-4784 and positively regulated by LBX2-AS1 in terms of expression level. Upregulation of KDM5C reversed the inhibitory effect of LBX2-AS1 depletion on the progression of OC. This study proved that ELK1 activated-LBX2-AS1 aggravated the progression of OC by targeting the miR-4784/KDM5C axis, suggesting that LBX2-AS2 may be a promising diagnostic biomarker of OC.
journal_name
J Mol Histoljournal_title
Journal of molecular histologyauthors
Gu H,Lin R,Zheng F,Zhang Qdoi
10.1007/s10735-020-09921-5subject
Has Abstractpub_date
2021-02-01 00:00:00pages
31-44issue
1eissn
1567-2379issn
1567-2387pii
10.1007/s10735-020-09921-5journal_volume
52pub_type
杂志文章abstract：:Multidrug resistance-associated protein 1 (MRP1) belongs to ATP-binding cassette transporters family. The overexpression of MRP1 is predominantly related with the failure of chemo-radiotherapy in various tumors. However, its possible role in hypertrophic scar (HS) is hardly investigated. Here we showed that the mRNA l...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-015-9629-0
更新日期：2015-10-01 00:00:00
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journal_title：Journal of molecular histology
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doi：10.1007/s10735-018-9780-5
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doi：10.1007/s10735-013-9557-9
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journal_title：Journal of molecular histology
pub_type： 杂志文章
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abstract：:MI-ER1 is a novel transcriptional regulator that plays a critical role in embryonic development and is differentially expressed in breast carcinoma. The MI-ER1 protein sequence is highly conserved among species, with 95% identity between mouse and humans and 72% between Xenopus and mouse. There are two major protein i...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9116-3
更新日期：2008-02-01 00:00:00
abstract：:Spatio-temporal immunolocalizations of cytokeratin 8 (CK8), vimentin, syndecan-1 and Ki-67 were analyzed in ten human incisors and canine tooth germs between the 7th and 20th developmental weeks. CK8 expression was mild to moderate in the epithelial tooth parts, while it shifted from absent or mild in its mesenchymal ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-014-9592-1
更新日期：2014-12-01 00:00:00
abstract：:Chronic stress is regarded as a significant factor in the etiology of the many diseases. Numerous methods have been developed through which the effect of chronic stress is examined. The aim of this study is to demonstrate the new experimental model for analysis of immuno-suppression induced by chronic restraint stress...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09886-5
更新日期：2020-08-01 00:00:00
abstract：:The distribution of molecular components of interendothelial tight junctions (TJs) was studied in rat blood-brain barrier (BBB) microvessels, using immunogold cytochemistry applied to electron microscopy. Samples of rat brains, both normal (unaffected) and osmotically-affected (1, 5, and 30 min after intracarotid infu...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/10.1007/s10735-004-1318-3
更新日期：2004-06-01 00:00:00
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journal_title：Journal of molecular histology
pub_type： 杂志文章,已发布勘误
doi：10.1007/s10735-018-9783-2
更新日期：2018-08-01 00:00:00
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journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09850-y
更新日期：2019-12-01 00:00:00
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journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-004-2653-0
更新日期：2004-09-01 00:00:00
abstract：:Prostate cancer is the most common cancer in American men and the second leading cause of cancer deaths in this group. Both growth hormone (GH) and the inflammatory cytokine interleukin 6 (IL-6) have been implicated in prostate cancer progression. Studies in other systems have shown that an increase in GH results in a...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-006-9027-8
更新日期：2006-01-01 00:00:00
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journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9117-2
更新日期：2007-10-01 00:00:00
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journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9123-4
更新日期：2007-10-01 00:00:00
abstract：:The aim of this study was to investigate oxidative DNA damage during 4-nitroquinoline 1-oxide (4NQO)-induced rat tongue carcinogenesis. For this purpose, male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12, and 20 weeks. Te...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-011-9323-9
更新日期：2011-04-01 00:00:00
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journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-017-9731-6
更新日期：2017-12-01 00:00:00
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journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09879-4
更新日期：2020-06-01 00:00:00
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journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-009-9218-1
更新日期：2009-04-01 00:00:00
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journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-008-9173-2
更新日期：2008-08-01 00:00:00
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journal_title：Journal of molecular histology
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doi：10.1007/s10735-016-9709-9
更新日期：2017-04-01 00:00:00
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journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-008-9176-z
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journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09918-0
更新日期：2020-12-01 00:00:00
abstract：:MicroRNAs are small noncoding RNAs involved in various biological processes. We characterized the expression of miR-344-3p during mouse embryonic development. At E9.5-E10.5 and E15.5, in situ hybridization detected strong miR-344-3p signal in the central nervous system, including the cerebral cortex, hindbrain, cerebe...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9555-y
更新日期：2014-08-01 00:00:00
abstract：:With the marker of Paneth cells-lysozyme, secretory component (SC) immunoreactivity was demonstrated exclusively in Paneth cells of rat small intestine. The other types of epithelial cells (columnar, goblet, endocrine) were negative. On electron microscopic level, many SC-positive colloidal gold particles were found i...
journal_title：Journal of molecular histology
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doi：10.1007/s10735-005-9003-8
更新日期：2005-06-01 00:00:00
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journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9134-1
更新日期：2008-02-01 00:00:00
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journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-011-9336-4
更新日期：2011-08-01 00:00:00
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journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-005-2255-5
更新日期：2005-03-01 00:00:00
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journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-006-9050-9
更新日期：2006-05-01 00:00:00
abstract：:The members of high-temperature requirement (HtrA) family are evolutionarily conserved serine proteases that combine a trypsin-like protease domain with at least one PDZ interaction domain. HtrA2, a special one, is mainly located in mitochondria and required for maintaining homeostasis. Once released into cytoplasm, H...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9489-4
更新日期：2013-08-01 00:00:00
abstract：:In the last few decades, several growth factors were identified in the testis of various mammalian species. Growth factors are shown to promote cell proliferation, regulate tissue differentiation, and modulate organogenesis. In the present investigation we have studied the localization of EGF and EGFR in the adult bov...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9089-2
更新日期：2007-06-01 00:00:00
