Abstract：
:Previous studies have shown aberrant expression of ubiquitin-specific protease 14 (USP14) in multiple malignancies, suggesting an important role of USP14 in tumorigenesis. However, the functional role of USP14 in pancreatic ductal adenocarcinoma (PDAC) has never been elucidated. In this study, we found that USP14 was remarkably upregulated in PDAC tissues compared with normal pancreatic tissues. Notably, Kaplan-Meier curves showed that high expression of USP14 predicted significantly worse prognosis in PDAC patients than low expression of USP14. To determine whether USP14 could regulate the proliferation, apoptosis and metastasis of PDAC cells, we knocked down endogenous USP14 or overexpressed exogenous USP14 in Panc-1 and BxPC-3 cells. Using MTT assays, colony formation analyses, flow cytometry assays, and cell invasion and migration assays, we found that knockdown of USP14 attenuated proliferation, induced apoptosis and restrained invasion and migration of PDAC cells. Overexpression of USP14 could enhance proliferation, prevent apoptosis and promote invasion and migration of PDAC cells. In addition, USP14 could regulate the expression of cyclin D1, PCNA and E-cadherin, three important carcinogenic factors, in PDAC cells. These findings suggest that USP14 might play an important role in promoting the tumorigenesis of PDAC and thus be a promising therapeutic target to prevent PDAC progression.
journal_name
J Mol Histoljournal_title
Journal of molecular histologyauthors
Hang C,Gong C,Fang Y,Chen L,Zhu Jdoi
10.1007/s10735-020-09934-0subject
Has Abstractpub_date
2021-01-04 00:00:00eissn
1567-2379issn
1567-2387pii
10.1007/s10735-020-09934-0pub_type
杂志文章abstract：:Vascular endothelial growth factor (VEGF) is an important angiogenic factor in the pituitary gland. The objective of this study was to unveil the VEGF subcellular localisation in different pituitary cell types and to evaluate changes in its expression at different time intervals after oestrogen stimulation. A relevant...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-005-9012-7
更新日期：2005-10-01 00:00:00
abstract：:In in vitro and in vivo systems, understanding localization and the functional role of ATP is essential, but effective methods to monitor ATP in cells and tissues are limited. Although quinacrine dihydrochloride is a well-known fluorescent dye used to detect ATP, it is limited in its use because it shows non-specific ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-012-9474-3
更新日期：2013-04-01 00:00:00
abstract：:The first cell lineage differentiation occurs during the development of mouse 8-cell embryo to blastocyst. Akt is a potent kinase whose role during blastocyst formation has not been elucidated. In the present study, immunofluorescence results showed that the Akt protein was specifically localized to the outer cells of...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09833-z
更新日期：2019-08-01 00:00:00
abstract：:Vesicular monoamine transporter 2 (VMAT2) is expressed in pancreatic beta cells and has recently been proposed as a target for measurement of beta cell mass in vivo. We questioned, (1) What proportion of beta cells express VMAT2? (2) Is VMAT2 expressed by other pancreatic endocrine or non-endocrine cells? (3) Is the r...
journal_title：Journal of molecular histology
pub_type： 杂志文章,多中心研究
doi：10.1007/s10735-008-9195-9
更新日期：2008-10-01 00:00:00
abstract：:Some members of aquaporin family (AQP) plays crucial functions in salivary synthesis and secretion. These proteins expression has already been reported during salivary gland formation, however no previous studies in human developing glands have been performed. We evaluated AQP1, 3 and 5 expression through the stages o...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-017-9731-6
更新日期：2017-12-01 00:00:00
abstract：:Objective Oligodendroglial tumors with a loss of heterozygosity of 1p (LOH1p) appear to have a better prognosis than oligodendrogliomas without LOH. Previously, we reported glucose uptake in low-grade oligodendroglial tumors to be related to LOH 1p status. Here, we performed an immunohistochemical study of the common ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-008-9191-0
更新日期：2008-10-01 00:00:00
abstract：:As one of the most common cancers in female, ovarian cancer (OC) has become a serious public burden now. Mounting researches have indicated long noncoding RNAs (lncRNAs) can affect many biological processes including cancer development. LncRNA LBX2-AS1 was identified to be an oncogene in some cancers, but the role of ...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09921-5
更新日期：2021-02-01 00:00:00
abstract：:The neurovascular structures in the cranial dura mater have been studied with various histological techniques in the past years. In order to obtain a proper approach to reveal the detailed structures, different labeling methods for the cranial vessels and nerve fibers were tested in this study. Firstly, the labeling c...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09903-7
更新日期：2020-10-01 00:00:00
abstract：:Pulp regeneration using human dental pulp stem cells (hDPSCs) maintains tooth vitality compared with conventional root canal therapy. Our previous study demonstrated that preameloblast-conditioned medium (PA-CM) from murine apical bud cells induces the odontogenic differentiation of hDPSCs and promoted dentin formatio...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9513-8
更新日期：2013-12-01 00:00:00
abstract：:Inpp5f-v3 is a transcriptional variant of Inpp5f (inositol polyphosphate-5-phosphatase F) and locates in distal mouse chromosome 7. It is a paternally expressed imprinted gene in mouse. In this study, we examined the spatiotemporal patterns of Inpp5f-v3 gene during the mouse development. The northern blotting analysis...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-011-9321-y
更新日期：2011-04-01 00:00:00
abstract：:Adult neurogenesis is a characteristic feature of the olfactory pathways of decapod crustaceans. In crayfish and clawed lobsters, adult-born neurons are the progeny of precursor cells with glial characteristics located in a neurogenic niche on the ventral surface of the brain. The daughters of these precursor cells mi...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9112-7
更新日期：2007-12-01 00:00:00
abstract：:p21-activated Kinase 6 (PAK6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. PAK kinases are well-known regulators of a wide variety of cellular functions, including regulation of cytoskeleton rearrangement, cell survival, apoptosis and the mitogen-activated protein kinase signaling p...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-011-9324-8
更新日期：2011-06-01 00:00:00
abstract：:Despite the efficacy of tamoxifen in preventing disease relapse, a large portion of breast cancer patients show intrinsic or acquired resistance to tamoxifen, leading to treatment failure and unfavorable clinical outcome. MYB proto-oncogene like 2 (MYBL2) is a transcription factor implicated in the initiation and prog...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09920-6
更新日期：2021-02-01 00:00:00
abstract：:The natriuretic peptide (NP) system comprises of three ligands, the Atrial Natriuretic Peptide (ANP), Brain Natriuretic peptide (BNP) and C-type Natriuretic peptide (CNP), and three natriuretic peptide receptors, NPRA, NPRB and NPRC. Here we present a comprehensive study of the natriuretic peptide system in healthy mu...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09849-5
更新日期：2020-02-01 00:00:00
abstract：:Epidermal adult stem cells (EpiASCs) have the potential for unlimited proliferation and differentiation, however, the ability of these stem cells to activate corneal genetic programs in response to corneal stroma stimulation needs to be further validated. Herein, a feasible strategy was developed to reconstruct the da...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-020-09879-4
更新日期：2020-06-01 00:00:00
abstract：:Four and a half LIM domains 2 (FHL2) is a protein of 279 amino acids in length containing four full LIM-domains and a half LIM-domain at the amino terminus. FHL2 is one transcriptional cofactor that can interact with many different proteins, such as AP-1, BRCA1, IGFBP, and integrin, and involved in organ differentiati...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-016-9654-7
更新日期：2016-04-01 00:00:00
abstract：:Wnt signaling plays important roles in skeletal development. However, the activation and function of canonical Wnt signaling in joint development remains unclear. We analyzed the lineage identity and developmental changes of the Wnt-responsive cells during synovial joint formation as well as adulthood in the Wnt signa...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-009-9242-1
更新日期：2009-08-01 00:00:00
abstract：:Previous findings have shown that P2X-purinoceptor-mediated signaling pathways regulate the release of ACh in the retina. We previously reported the existence of immunoreactivity for P2X1-, P2X2-, P2X4-, and P2X7-purinoceptors in mouse retina and speculated that P2X2 and P2X7-purinoceptors may modulate the activity of...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-007-9107-4
更新日期：2007-08-01 00:00:00
abstract：:Quantitative immunogold procedure was used to study the distribution of molecular components of interendothelial junctions in blood-brain barrier (BBB) microvessels of scrapie infected SJL/J hyperglycemic mice showing obesity and reduced glucose tolerance. Samples of brain (fronto-parietal cerebral cortex and thalamo-...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-006-9026-9
更新日期：2006-01-01 00:00:00
abstract：:Polycystic ovary syndrome (PCOS) is a major health problem in reproductive-aged women worldwide, but the precise pathogenesis of PCOS remains unclear. Our previous study revealed that hypoxia-inducible factor (HIF)-1a mediated endothelin (ET)-2 signaling plays an important role in ovulation in rats. Therefore, the pre...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-015-9609-4
更新日期：2015-04-01 00:00:00
abstract：:Microscopy is advancing at a rapid pace, enabling high-speed, high-resolution analyses to be conducted in a wide range of cellular contexts. For example, the capacity to quickly capture high-resolution images from multiple optical sections over multiple channels with confocal microscopy has allowed researchers to gain...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09850-y
更新日期：2019-12-01 00:00:00
abstract：:The aim of this study was to ascertain whether, like many cell types in cartilaginous tissues if type XI collagen was a pericellular component of annulus fibrosus (AF) cells and chondrocytes. Fine fibrillar networks were visualised which were perlecan, HS (MAb 10E4) and type XI collagen positive. Heparitinase-III pre-...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09823-1
更新日期：2019-06-01 00:00:00
abstract：:Submandibular gland (SMG) autotransplantation is an effective therapy for treating severe dry eye syndrome. However, epiphora occurs in more than 40% of patients 6 months after operation. We previously found that muscarinic acetylcholine receptor (mAChR) plays a crucial role in regulating SMG secretion partially throu...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-016-9709-9
更新日期：2017-04-01 00:00:00
abstract：:The aim of this study was to investigate oxidative DNA damage during 4-nitroquinoline 1-oxide (4NQO)-induced rat tongue carcinogenesis. For this purpose, male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12, and 20 weeks. Te...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-011-9323-9
更新日期：2011-04-01 00:00:00
abstract：:E3 ubiquitin ligases regulate a variety of biological processes through the ubiquitin-proteasome system, together with ubiquitin activating enzyme E1 and ubiquitin-conjugating enzyme E2. Previous studies have demonstrated that zinc and ring finger 3 (ZNRF3), which belongs to the E3 ubiquitin ligases family is involved...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-013-9504-9
更新日期：2013-10-01 00:00:00
abstract：:Nel-like molecule-1 (NELL-1) is a novel highly specific growth factor that can induce osteoblast differentiation and bone formation as well as odontoblast differentiation. Recent studies have suggested that NELL-1 can synergistically increase bone formation and regeneration with bone morphogenetic protein 2 (BMP2) and...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09822-2
更新日期：2019-06-01 00:00:00
abstract：:Stromal cells in the peripheral zone (PZ) of the prostate from older males (PZ-old) could significantly promote Prostate cancer (PCa) growth compared with stromal cells from young males (PZ-young). But the mechanism is still unknown. In the co-culture system with PZ-old cells, Pc3/Du145 cells showed advanced prolifera...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-016-9679-y
更新日期：2016-06-01 00:00:00
abstract：:Dentin is a major component of teeth that protects dental pulp and maintains tooth health. Bone morphogenetic protein (BMP) signaling is required for the formation of dentin. Mice lacking a BMP type I receptor, activin A receptor type 1 (ACVR1), in the neural crest display a deformed mandible. Acvr1 is known to be exp...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-018-9806-z
更新日期：2019-02-01 00:00:00
abstract：:The accurately establishment of the eruptive pathway is of vital importance. The mechanisms governing tooth eruption pathway remain little known. This study is to elucidate the roles of Semaphorin 3A (Sema 3A) in mouse tooth eruptive pathway. C57BL/6 mice (11-13 and 15-17 days after birth) were chosen to observe erupt...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-019-09838-8
更新日期：2019-10-01 00:00:00
abstract：:Sphingosine-1-phosphate receptor 1 (S1P(1)), a receptor for sphingosine-1-phosphate, has been shown to play an important role in the migration, proliferation, and survival of several types of cell including endothelial cells. Given that S1P(1) signaling could serve as a therapeutic target, we evaluate the expression o...
journal_title：Journal of molecular histology
pub_type： 杂志文章
doi：10.1007/s10735-008-9193-y
更新日期：2008-10-01 00:00:00
