Abstract:
:Hyperglycemia is associated with impairment of heart function. The current study aimed to investigate the ameliorative effect of polydatin-loaded chitosan nanoparticles (PD-CSNPs), polydatin (PD) and metformin (MET) on diabetic cardiomyopathy in rats. Rats divided into six groups; normal-control, diabetic-control, diabetic + CSNPs (diabetic rats treated with 50 mg/kg blank chitosan nanoparticles), diabetic + PD-CSNPs (diabetic rats treated with PD-CSNPs equivalent to 50 mg/kg of polydatin), diabetic + PD (diabetic rats given 50 mg/kg polydatin), diabetic + MET (diabetic rats given 100 mg/kg metformin), orally and daily for 4 weeks. Treatment of diabetic rats with PD-CSNPs, PD and MET showed a significant reduction in the values of glucose and glycosylated hemoglobin with improvement in heart function biomarkers through decreasing serum creatine kinase and creatine kinase myocardial band activities compared to diabetic control. The treatment agents also suppressed the elevated lipid peroxidation product, increased values of glutathione content, superoxide dismutase, superoxide peroxidase, and catalase activities in the heart of diabetic treated rats. Furthermore, PD-CSNPs, PD and MET decreased heart tissue levels of a pro-inflammatory cytokine; tumor necrosis factor-alpha and nuclear factor-kappa β, upregulation of heart gene expressions; nuclear factor erythroid 2-related factor 2 and heme oxygenase-1. Histological and ultrastructural examinations revealed the ameliorative effect of PD-CSNPs, PD and MET against the harmful of diabetic cardiomyopathy by reducing the cardiac fibers, necrotic cardiac myocytes, inflammatory cell infiltration, and the arrangement of the myofibrils and intercalated discs. In conclusion, the new formula of PD-CSNPs was more effective than PD and MET in amelioration the diabetic cardiomyopathy through its antioxidant, anti-inflammatory and prolonged-release properties.
journal_name
J Mol Histoljournal_title
Journal of molecular histologyauthors
Mostafa F,Abdel-Moneim A,Abdul-Hamid M,Galaly SR,Mohamed HMdoi
10.1007/s10735-020-09930-4subject
Has Abstractpub_date
2021-01-03 00:00:00eissn
1567-2379issn
1567-2387pii
10.1007/s10735-020-09930-4pub_type
杂志文章abstract::Inpp5f-v3 is a transcriptional variant of Inpp5f (inositol polyphosphate-5-phosphatase F) and locates in distal mouse chromosome 7. It is a paternally expressed imprinted gene in mouse. In this study, we examined the spatiotemporal patterns of Inpp5f-v3 gene during the mouse development. The northern blotting analysis...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-011-9321-y
更新日期:2011-04-01 00:00:00
abstract::Vesicular monoamine transporter 2 (VMAT2) is expressed in pancreatic beta cells and has recently been proposed as a target for measurement of beta cell mass in vivo. We questioned, (1) What proportion of beta cells express VMAT2? (2) Is VMAT2 expressed by other pancreatic endocrine or non-endocrine cells? (3) Is the r...
journal_title:Journal of molecular histology
pub_type: 杂志文章,多中心研究
doi:10.1007/s10735-008-9195-9
更新日期:2008-10-01 00:00:00
abstract::p53 plays a pivotal role in the prevention of human tumor formation. p73 and p63 are new members of the p53 tumor suppressor family, which are becoming increasingly recognized as important players in human tumorigenesis. However, the roles of these proteins are not well elucidated in extrahepatic bile duct (EBD) carci...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-007-9084-7
更新日期:2007-06-01 00:00:00
abstract::DLBCL is the most common lymphoma subtype occurring in older populations as well as in younger HIV infected patients. The current treatment options for DLBCL are effective for most patients yet the relapse rate is high. While many biomarkers for DLBCL exist, they are not in clinical use due to low sensitivity and spec...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-016-9695-y
更新日期:2016-12-01 00:00:00
abstract::Five subtypes of dopamine receptor exist in two subfamilies: two D(1)-like (D(1) and D(5)) and three D(2)-like (D(2), D(3) and D(4)). We produced novel monoclonal antibodies against all three D(2)-like receptors and used them to localize receptors in Ntera-2 (NT-2) cells, the human neuronal precursor cell line. Most o...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-007-9106-5
更新日期:2007-08-01 00:00:00
abstract::Stromal cells in the peripheral zone (PZ) of the prostate from older males (PZ-old) could significantly promote Prostate cancer (PCa) growth compared with stromal cells from young males (PZ-young). But the mechanism is still unknown. In the co-culture system with PZ-old cells, Pc3/Du145 cells showed advanced prolifera...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-016-9679-y
更新日期:2016-06-01 00:00:00
abstract::In order to study the presence of sulphated glycoconjugates in the first mineralised layer juxtaposed to the root dentine (the hyaline layer), we have examined the early stages of molar root development by ultrastructural cytochemistry using Cuprolinic Blue combined with enzymatic pretreatment. Upper molars from 10 to...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1023/b:hijo.0000021089.75408.7c
更新日期:2004-01-01 00:00:00
abstract::In the original publication of the article, two errors were made in describing the equations for Tc. ...
journal_title:Journal of molecular histology
pub_type: 杂志文章,已发布勘误
doi:10.1007/s10735-018-9783-2
更新日期:2018-08-01 00:00:00
abstract::Polycystic ovary syndrome (PCOS) is a major health problem in reproductive-aged women worldwide, but the precise pathogenesis of PCOS remains unclear. Our previous study revealed that hypoxia-inducible factor (HIF)-1a mediated endothelin (ET)-2 signaling plays an important role in ovulation in rats. Therefore, the pre...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-015-9609-4
更新日期:2015-04-01 00:00:00
abstract::During human pregnancy the placenta produces a variety of proteins like steroid hormones and their receptors that are responsible for the establishment and ongoing of the feto-placental unit. Inhibins are dimeric glycoproteins, composed of an alpha-subunit and one of two possible beta-subunits (beta (A) or beta (B)). ...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-006-9029-6
更新日期:2006-01-01 00:00:00
abstract::Nickel nanoparticles (Ni NPs) have been applied in various fields along with the rapid development of nanotechnology. However, the potential adverse health effects of the Ni NPs are unclear. To investigate the cyto- and genotoxicity and compare the differences between the Ni NPs and the nickel fine particles (Ni FPs),...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-016-9671-6
更新日期:2016-06-01 00:00:00
abstract::Follistatin like-1 (Fstl1) is a secreted glycoprotein and can be up-regulated by TGF-β1. To better study the function of Fstl1 in lung development, we examined Fstl1 expression in the developing lung, in a cell type specific manner, using a tamoxifen inducible Fstl1-reporter mouse strain. Our results show that Fstl1 i...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-018-9780-5
更新日期:2018-08-01 00:00:00
abstract::Selective cyclooxygenase-2 non-steroidal anti-inflammatory drugs are known to inhibit bone repair, especially when long-term administration is required due to chronicle inflammatory diseases. In order to evaluate the action of this drug in bone repair during short-term administration, 48 rats underwent surgical bone d...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-008-9176-z
更新日期:2008-08-01 00:00:00
abstract::Histochemical and morphological research increasingly relies upon quantification of complex biological systems. For such investigations to be meaningful, quantification techniques must meet the seemingly conflicting requirements of being theoretically robust, yet sufficiently practical to facilitate widespread applica...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-004-2187-5
更新日期:2004-08-01 00:00:00
abstract::Odontogenic keratocysts (OKCs) are jaw cystic lesions which are characterized by local invasion and high recurrence rate. The majority of OKCs are exposed to microorganisms and occur along with focal inflammatory infiltrates. Cyst fluids are biological fluids that contain a large content of cytokines and immune globul...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-019-09829-9
更新日期:2019-08-01 00:00:00
abstract::Immunohistochemical and fluorescent in situ hybridization (FISH) assays are useful diagnostic methods for the identification of bacteria on formalin fixed paraffin embedded histological sections. To validate an anti-bacterial antibody or an oligonucleotide probe and to ensure fidelity during subsequent analyses, suita...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-008-9173-2
更新日期:2008-08-01 00:00:00
abstract::Dentin is a major component of teeth that protects dental pulp and maintains tooth health. Bone morphogenetic protein (BMP) signaling is required for the formation of dentin. Mice lacking a BMP type I receptor, activin A receptor type 1 (ACVR1), in the neural crest display a deformed mandible. Acvr1 is known to be exp...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-018-9806-z
更新日期:2019-02-01 00:00:00
abstract::Satellite glial cells (SGCs), a peripheral neuroglial cell, surround neurons and form a complete envelope around individual sensory neurons in the trigeminal ganglia (TG), which may be involved in modulating neurons in inflammation. The purpose of this study was to determine the effect of dental injury and inflammatio...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-018-9765-4
更新日期:2018-06-01 00:00:00
abstract::Under normal homeostasis, melanocyte growth and behaviour is tightly controlled by the surrounding keratinocytes. Keratinocytes regulate melanocyte behaviour through a complex system of paracrine growth factors and cell-cell adhesion molecules. Pathological changes, leading to development of malignant melanoma, upset ...
journal_title:Journal of molecular histology
pub_type: 杂志文章,评审
doi:10.1023/b:hijo.0000032362.35354.bb
更新日期:2004-03-01 00:00:00
abstract::Tissue microarrays have become an essential tool in translational pathology. They are used to confirm results from other experimental platforms, such as expression microarrays, as well as a primary tool to explore the expression profile of proteins by immunohistochemical analysis. Tissue microarrays are routinely used...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-006-9049-2
更新日期:2007-05-01 00:00:00
abstract::Prostate cancer is the most common cancer in American men and the second leading cause of cancer deaths in this group. Both growth hormone (GH) and the inflammatory cytokine interleukin 6 (IL-6) have been implicated in prostate cancer progression. Studies in other systems have shown that an increase in GH results in a...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-006-9027-8
更新日期:2006-01-01 00:00:00
abstract::Cyclin G1 plays an essential role in the development of human carcinoma. Here, we characterized the clinical significance of Cyclin G1 and investigated its role in cellular proliferation and apoptosis of epithelial ovarian cancer (EOC). Western blot was used to evaluate the expression of Cyclin G1 in nine fresh EOC ti...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-015-9622-7
更新日期:2015-06-01 00:00:00
abstract::Objective Oligodendroglial tumors with a loss of heterozygosity of 1p (LOH1p) appear to have a better prognosis than oligodendrogliomas without LOH. Previously, we reported glucose uptake in low-grade oligodendroglial tumors to be related to LOH 1p status. Here, we performed an immunohistochemical study of the common ...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-008-9191-0
更新日期:2008-10-01 00:00:00
abstract::In the last few decades, several growth factors were identified in the testis of various mammalian species. Growth factors are shown to promote cell proliferation, regulate tissue differentiation, and modulate organogenesis. In the present investigation we have studied the localization of EGF and EGFR in the adult bov...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-007-9089-2
更新日期:2007-06-01 00:00:00
abstract::The distribution of molecular components of interendothelial tight junctions (TJs) was studied in rat blood-brain barrier (BBB) microvessels, using immunogold cytochemistry applied to electron microscopy. Samples of rat brains, both normal (unaffected) and osmotically-affected (1, 5, and 30 min after intracarotid infu...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/10.1007/s10735-004-1318-3
更新日期:2004-06-01 00:00:00
abstract::S100A8 and S100A9 are calcium-binding proteins expressed in myeloid cells and are markers of numerous inflammatory diseases in humans. S100A9 has been associated with dystrophic calcification in human atherosclerosis. Here we demonstrate S100A8 and S100A9 expression in murine and human bone and cartilage cells. Only S...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-007-9117-2
更新日期:2007-10-01 00:00:00
abstract::Despite the efficacy of tamoxifen in preventing disease relapse, a large portion of breast cancer patients show intrinsic or acquired resistance to tamoxifen, leading to treatment failure and unfavorable clinical outcome. MYB proto-oncogene like 2 (MYBL2) is a transcription factor implicated in the initiation and prog...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-020-09920-6
更新日期:2021-02-01 00:00:00
abstract:AIM:To compare the expression of p57 as indirect marker of genomic imprinting of CDKN1C in a series of infantile hemangiomas (IH) of patients with and without Beckwith-Wiedemann syndrome. MATERIALS AND METHODS:Cases of mammary, salivary gland, liver (one each), and placental (2 cases) capillary hemangiomas all with hi...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-005-2255-5
更新日期:2005-03-01 00:00:00
abstract::During transmyocardial revascularization, cellular destruction of cardiomyocytes occurs as a result of the high-energy laser. However, the features of myocardial cellular destruction are unclear. The present study was undertaken to examine the structural characteristics of cell death in the myocardium following transm...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-005-5343-7
更新日期:2005-05-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is an important angiogenic factor in the pituitary gland. The objective of this study was to unveil the VEGF subcellular localisation in different pituitary cell types and to evaluate changes in its expression at different time intervals after oestrogen stimulation. A relevant...
journal_title:Journal of molecular histology
pub_type: 杂志文章
doi:10.1007/s10735-005-9012-7
更新日期:2005-10-01 00:00:00