Abstract：
:A monoclonal antibody (Mab) developed against a partially purified bursal protein extract was found to bind specifically to a single cell type in the cortico-medullary border region of the chicken bursa of Fabricius. These cells were microscopically similar to the bursal secretory dendritic-like cells. A product with an apparent molecular weight of approximately 56 kDa on SDS-polyacrylamide gel electrophoresis was immunopurified from bursal extracts by utilizing this Mab. This product was subjected to peptide digestion and protein sequencing. The two resulting sequences perfectly matched the known sequence of chicken ovoinhibitor. Gene-specific polymerase chain reaction (PCR) primers were designed for the ovoinhibitor, RNA was purified from chicken bursae, and reverse transcription/PCR was performed. Two amplicons with the expected size for ovoinhibitor mRNA were obtained. These data suggest that the gene for ovoinhibitor is expressed in the bursa of Fabricius, and that the bursal secretory dendritic-like cells may be a previously unreported source of ovoinhibitor.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Moore RW,Hargis BM,Porter TE,Caldwell DY,Oubre CM,Vandesande F,Berghman LRdoi
10.1007/s00441-004-0910-xkeywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
247-51issue
3eissn
0302-766Xissn
1432-0878journal_volume
317pub_type
杂志文章abstract：:Type 1 diabetes is an autoimmune disorder characterized by a lack of insulin production by the beta cells of the pancreas. This lack of insulin causes a variety of systemic effects on whole-body metabolism. Poorly managed type 1 diabetes can lead to cardiovascular disease, diabetic neuropathy, and diabetic retinopathy...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-012-1387-7
更新日期：2012-09-01 00:00:00
abstract：:Stem cells are an attractive cellular source for regenerative medicine and tissue engineering applications due to their multipotency. Although the elasticity of the extracellular matrix (ECM) has been shown to have crucial impacts in directing stem cell differentiation, it is not the only contributing factor. Many res...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-015-2190-z
更新日期：2015-09-01 00:00:00
abstract：:SCO-spondin is a newly identified protein, strongly expressed in the subcommissural organ (SCO), an ependymal differentiation of the brain. When secreted into the cerebrospinal fluid at the entrance to the Sylvian aqueduct, it condenses and forms Reissner's fiber. Several conserved domains have previously been charact...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051132
更新日期：1998-09-01 00:00:00
abstract：:Transformation of the gastrula to the pluteus includes development of the ability of the larva to control the direction of ciliary beat and coordinate activities of the ciliary band with activities of the esophageal muscles (48-60 h, 15 degrees C). Glyoxylic acid-induced fluorescence shows several cells of the animal ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217887
更新日期：1983-01-01 00:00:00
abstract：:Fluorescent (aminergic) fibers were demonstrated by the Falck-Hillarp technique in all parts of the neurohypophysis (NH) in the roach (Leuciscus rutilus). The fibers are very thin and few in number in the rostral (RNH) and proximal neurohypophysis (PNH) but slightly more numerous in the NH adjacent to the pars interme...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219959
更新日期：1975-01-01 00:00:00
abstract：:The surface coat of the electrocyte of the main electric organ of Electrophorus electricus was studied using cytochemical methods (periodic acid-silver methanamine, periodic acid-chromic acid-silver methenamine, periodic acid-thiosemicarbazide-silver proteinate, Concanavalin A - horseradish peroxidase, ruthenium red, ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00226622
更新日期：1977-09-26 00:00:00
abstract：:In the current experiment, the combined regime of resveratrol and a Wnt-3a inhibitor, sulindac, were examined on the angiogenic potential of cancer stem cells from human colon adenocarcinoma cell line HT-29 during 7 days. Cancer stem cells were enriched via a magnetic-activated cell sorter technique and cultured in en...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-02998-9
更新日期：2019-06-01 00:00:00
abstract：:Young rats were injected with 3H-fucose and killed at 10 min, 1, 4 and 24 h thereafter. Samples of the pancreas were processed for light- and electron-microscopic radioautography and then analyzed quantitatively. Fucose label was taken up in the supranuclear region of acinar pancreatic cells that were maximally labele...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222243
更新日期：1983-01-01 00:00:00
abstract：:Whether an axon will regenerate after it is cut depends on the balance between the intrinisic ability of the axon to regrow and the permissiveness of the environment surrounding it. The permissiveness of the environment is determined by the glial cells at the site of injury, and in the CNS both oligodendrocytes and as...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s004410050943
更新日期：1997-11-01 00:00:00
abstract：:The present study describes substance P-like immunoreactivity in the ciliary ganglia of monkey (Macaca fascicularis) and cat. About 60% of neurons in the monkey ciliary ganglion and 40% in the cat ciliary ganglion were substance P-like immunoreactive, ranging from faint to moderate staining. Substance P-like immunorea...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00354796
更新日期：1994-04-01 00:00:00
abstract：:The extracellular matrix (ECM) acts as a critical factor during morphogenesis. Because the organization of the ECM directly influences the structure of tissues and organs, a determination of the way that ECM organization is regulated should help to clarify morphogenesis. We have analyzed the assembly of Del1, an ECM p...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0456-9
更新日期：2007-10-01 00:00:00
abstract：:By use of the indirect immunofluorescence technique a small group of large somatostatin-positive neurons is described in the subependymal area of the anterior paraventricular thalamus of the male rat. Retrograde-tracing experiments suggest that they project to areas outside the blood-brain barrier. ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215212
更新日期：1986-01-01 00:00:00
abstract：:Two types of mammosomatotropes (MS), the small-granule and vesicle-granule MS, were detected in mouse adenohypophysis by electron microscopy and immunohistochemistry. Both cell-types were immunoreactive to prolactin (PRL) and growth hormone (GH) antisera. The small-granule MS contained small, round, solid secretory gr...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00225615
更新日期：1989-06-01 00:00:00
abstract：:Immunocytochemical application of the antimuscarinic acetylcholine receptor antibody M35 to pancreas tissue revealed the target areas for the parasympathetic nervous system. Immunoreactivity in the endocrine pancreas was much higher than that in the exocrine part. Moreover, the endocrine cells at the periphery of the ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00384730
更新日期：1992-07-01 00:00:00
abstract：:Vitrification of biological specimens in liquid nitrogen can be achieved under high pressure (2,100 bars). This procedure obviates the use of aldehyde fixation and cryoprotection (glycerol). The present work demonstrates its applicability to the freeze-etching of mammalian brain tissue. Freeze-fracture repicas from ra...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00237626
更新日期：1980-01-01 00:00:00
abstract：:Ependymal cells located above the ventricular zone of the lateral, third, and fourth ventricles and the spinal cord are thought to form part of the adult neurogenic niche. Many studies have focused on ependymal cells as potential adult neural stem/progenitor cells. To investigate the functions of ependymal cells, a si...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-03132-5
更新日期：2020-03-01 00:00:00
abstract：:Larvae of Ascidia malaca, both before and after hatching, were treated with L-thyroxine solutions. The effect of the thyroid hormone was to induce the onset of metamorphosis and then to cause the rate at which body reorganization occurred to increase. In treated larvae the resorption of the tail occurred only few hour...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00249213
更新日期：1981-01-01 00:00:00
abstract：:While brain-derived neurotrophic factor (BDNF) messenger RNA (mRNA) has been localized in the developing gustatory epithelium, little information is available about neurotrophin-3 (NT-3) mRNA expression pattern in the prenatal developing gustatory and lingual epithelium. In the present study, using in situ hybridizati...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051092
更新日期：1998-06-01 00:00:00
abstract：:The haemomonochorial placenta of the guinea-pig undergoes several quantitative changes between the 49th and 64th days of gestation, all of which are in such a direction as to increase the efficiency of transplacental transport. The fetal vessels become larger, the maternal vessels increase in surface area by prolifera...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220974
更新日期：1977-11-23 00:00:00
abstract：:The present electron microscopic autoradiographic study includes a quantitative analysis of osteoclasts in vitro using tritiated leucin as a protein tracer. A significant increase in the grain density over the ruffled border and the underlying resportion zone was demonstrated two hours post pulse whereas the grain den...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219726
更新日期：1976-04-28 00:00:00
abstract：:The size of various tubes within tubular organs such as the lung, vascular system and kidney must be finely tuned for the optimal delivery of gases, nutrients, waste and cells within the entire organism. Aberrant tube sizes lead to devastating human illnesses, such as polycystic kidney disease, fibrocystic breast dise...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-013-1673-z
更新日期：2013-11-01 00:00:00
abstract：:The ultrastructure of the dermal papulae of a starfish (Asterias rubens) is consistent with a respiratory function. The present study has shown no regions specialized for excretory mechanisms. The papulae consist of an outer ectodermal epithelium of sensory, support and gland cells and a small basiepithelial nerve ple...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00229616
更新日期：1978-03-13 00:00:00
abstract：:Within seminiferous tubules, Sertoli cells form three types of actin-filament-containing cell junctions, viz., tight junctions, ectoplasmic specializations, and tubulobulbar complexes. These Sertoli cell junctions are involved in the formation of the blood-testis barrier, germ cell translocation, and the release of sp...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-004-0864-z
更新日期：2004-05-01 00:00:00
abstract：:Using anti-carp-gonadotropic-gamma-globulin, the indirect immunofluorescence technique was applied to sections of the pituitary of 12 teleost species. From the investigated species, the Poeciliinae, and especially the black molly, showed a distinct localization of fluorescent cells in the mesoadenohypophysis. Strong f...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224328
更新日期：1976-03-16 00:00:00
abstract：:A technique has been developed to obtain viable, isolated and enriched populations of gastrin cells (G-cells) from the rat stomach. Restricted tissue samples from a small area of the pyloric antrum known to be particularly rich in G-cells, were sequentially digested with pronase followed by mechanical agitation, to re...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236409
更新日期：1979-08-01 00:00:00
abstract：:The regression of atretic oocytes in Perca fluviatilis was studied by histochemical, light and electron microscopic methods. The course of regression can be divided into three stages, the first two comprising the dissolution of the atretic oocyte and its phagocytosis by the granulosa cells of the follicular epithelium...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00209978
更新日期：1981-01-01 00:00:00
abstract：:The cellular localization of the enzymes tyrosine hydroxylase (TH), aromatic amino-acid decarboxylase (or dopa decarboxylase, DDC), dopamine beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) in the adrenal medulla of adult rats and rat fetuses (14th, 17th, 18th, 19th and 21st day) was examined. ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00236882
更新日期：1979-08-03 00:00:00
abstract：:N-CAM180, the molecular form of the three neural cell adhesion molecules (N-CAM) with the largest cytoplasmic domain, is accumulated at sites of cell-cell contact (cell bodies, neurites, growth cones) in cultures of neuroblastoma and cerebellum. At these sites the cytoskeleton-membrane linker protein brain spectrin an...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214676
更新日期：1987-10-01 00:00:00
abstract：:It is known that human and animal fibroblasts are able to induce the retraction of a fibrin clot. In the present study the correlation between (i) fibrin-fibroblasts during growth, (ii) the number of actin stress-lines in mouse fibroblasts during growth in culture, and (iii) the sensitivity of actin stress-lines to a ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218485
更新日期：1982-01-01 00:00:00
abstract：:The present lectin histochemical comparison of cauda epididymal and ampullary bovine sperm was conducted to investigate whether ampullary secretions are involved in altering the plasma-membrane glycoconjugates of sperm. A marked redistribution of glycoconjugates between sperm from these two regions was indeed revealed...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318822
更新日期：1994-03-01 00:00:00
